Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,980,959 A→G T25A (ACC→GCC)  yifK → putative transporter YifK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,980,9590AG97.4% 1017.0 / 10.4 267T25A (ACC→GCC) yifKputative transporter YifK
Reads supporting (aligned to +/- strand):  ref base A (4/3);  new base G (141/119);  total (145/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

Only 100 of 267 total aligned reads displayed.

AGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTT  >  NC_000913/3980861‑3981056
                                                                                                  |                                                                                                 
 gCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCa                                                                                                >  2:6145144/1‑101 (MQ=255)
  cATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCAt                                                                                               <  1:6144764/101‑1 (MQ=255)
  cATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCAt                                                                                               >  2:6145146/1‑101 (MQ=255)
   aTGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCAtt                                                                                              <  1:6144595/101‑1 (MQ=255)
    tGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTg                                                                                             >  2:6145151/1‑101 (MQ=255)
       aaaCACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCg                                                                                          >  2:6145157/1‑101 (MQ=255)
       aaaCACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCg                                                                                          >  1:6145158/1‑101 (MQ=255)
       aaaCACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCg                                                                                          >  1:6145160/1‑101 (MQ=255)
         acacaacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTc                                                                                        <  1:6144778/101‑1 (MQ=255)
            caacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGc                                                                                     >  1:6145166/1‑101 (MQ=255)
             aacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGcc                                                                                    >  2:6145168/1‑101 (MQ=255)
               cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCgg                                                                                      <  1:6144646/97‑1 (MQ=255)
               cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTg                                                                                  >  2:6145171/1‑101 (MQ=255)
               cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTg                                                                                  <  1:6144687/101‑1 (MQ=255)
                acGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGt                                                                                 >  1:6145173/1‑101 (MQ=255)
                    ggATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTAt                                                                             <  1:6144718/101‑1 (MQ=255)
                    ggATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTAt                                                                             <  2:6144516/101‑1 (MQ=255)
                    ggATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTAt                                                                             >  2:6145178/1‑101 (MQ=255)
                     gATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATg                                                                            >  1:6145180/1‑101 (MQ=255)
                       tttATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATggg                                                                          >  2:6145182/1‑101 (MQ=255)
                         tATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATggggg                                                                        <  1:6144819/101‑1 (MQ=255)
                         tATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATggggg                                                                        <  1:6144637/101‑1 (MQ=255)
                          aTGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGgc                                                                       >  2:6145187/1‑101 (MQ=255)
                          aTGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGgc                                                                       <  2:6144628/101‑1 (MQ=255)
                          aTGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGgc                                                                       >  1:6145186/1‑101 (MQ=255)
                              cAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGgccgcc                                                                   >  2:6145191/1‑101 (MQ=255)
                               aGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa                                                                  <  1:6144575/101‑1 (MQ=255)
                                 aTAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGt                                                                >  2:6145194/1‑101 (MQ=255)
                                  tAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTa                                                               >  1:6145196/1‑101 (MQ=255)
                                   aaCAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAc                                                              >  2:6145200/1‑101 (MQ=255)
                                   aaCAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAc                                                              <  1:6144813/101‑1 (MQ=255)
                                   aaCAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAc                                                              >  1:6145199/1‑101 (MQ=255)
                                      aaaCCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCt                                                           <  1:6144858/101‑1 (MQ=255)
                                      aaaCCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCt                                                           >  1:6145204/1‑101 (MQ=255)
                                        aCCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGa                                                         >  1:6145207/1‑101 (MQ=255)
                                         ccAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaa                                                        <  1:6144522/101‑1 (MQ=255)
                                          cAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACACATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaaa                                                       >  1:6145209/1‑101 (MQ=255)
                                           agagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAAt                                                      >  2:6145212/1‑101 (MQ=255)
                                           agagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAAt                                                      <  2:6144685/101‑1 (MQ=255)
                                            gagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATg                                                     >  1:6145214/1‑101 (MQ=255)
                                             agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgg                                                    >  2:6145217/1‑101 (MQ=255)
                                             agCTACAGCGTGGGCTGGAAGCTAGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgg                                                    <  1:6144841/101‑1 (MQ=255)
                                               cTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggc                                                  >  2:6145220/1‑101 (MQ=255)
                                                tACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggcc                                                 >  1:6145222/1‑101 (MQ=255)
                                                 aCAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccg                                                <  1:6144936/101‑1 (MQ=255)
                                                 aCAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccg                                                >  1:6145225/1‑101 (MQ=255)
                                                   aGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccggg                                              <  2:6144762/101‑1 (MQ=255)
                                                     cGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc                                                             <  2:6144658/84‑1 (MQ=255)
                                                      gTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggcc                                            <  1:6144664/100‑1 (MQ=255)
                                                      gTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccg                                                <  2:6144726/96‑1 (MQ=255)
                                                      gTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCa                                           >  1:6145230/1‑101 (MQ=255)
                                                      gTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCa                                           <  2:6144773/101‑1 (MQ=255)
                                                        gggCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATc                                         <  2:6144756/101‑1 (MQ=255)
                                                        gggCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATc                                         >  2:6145232/1‑101 (MQ=255)
                                                          gCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCg                                       >  2:6145235/1‑101 (MQ=255)
                                                           cTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGt                                      >  1:6145239/1‑101 (MQ=255)
                                                           cTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGt                                      >  1:6145237/1‑101 (MQ=255)
                                                            tGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTa                                     >  2:6145241/1‑101 (MQ=255)
                                                            tGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTa                                     <  1:6144730/101‑1 (MQ=255)
                                                             ggAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAt                                    >  2:6145247/1‑101 (MQ=255)
                                                             ggAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAt                                    <  1:6144871/101‑1 (MQ=255)
                                                                 gCTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgtt                                >  1:6145251/1‑101 (MQ=255)
                                                                  cTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgttg                               >  2:6145253/1‑101 (MQ=255)
                                                                  cTCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgttg                               >  1:6145254/1‑101 (MQ=255)
                                                                   tCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGt                                                                <  1:6144706/67‑1 (MQ=255)
                                                                   tCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTgg                              >  1:6145257/1‑101 (MQ=255)
                                                                   tCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTgg                              <  1:6144642/101‑1 (MQ=255)
                                                                   tCGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTgg                              <  1:6144794/101‑1 (MQ=255)
                                                                    cGACATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGc                             <  1:6144768/101‑1 (MQ=255)
                                                                      aCATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCt                           <  2:6144877/101‑1 (MQ=255)
                                                                       cATATCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCta                          >  1:6145262/1‑101 (MQ=255)
                                                                         tatCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCtata                        >  1:6145265/1‑101 (MQ=255)
                                                                         tatCGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCtata                        >  2:6145264/1‑101 (MQ=255)
                                                                            cGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATatca                     >  2:6145268/1‑101 (MQ=255)
                                                                            cGAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATatca                     <  2:6144634/101‑1 (MQ=255)
                                                                             gAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATatcat                    <  1:6145049/101‑1 (MQ=255)
                                                                             gAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATatcat                    <  2:6144941/101‑1 (MQ=255)
                                                                             gAACTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATatcat                    <  2:6144705/101‑1 (MQ=255)
                                                                              aaCTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATatcatc                   >  1:6145271/1‑101 (MQ=255)
                                                                              aaCTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATatcatc                   <  2:6144821/101‑1 (MQ=255)
                                                                              aaCTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATatcatc                   <  2:6144800/101‑1 (MQ=255)
                                                                               aCTCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGCCCATCCGTATTGTTGGCCTATATCATCg                  <  1:6144742/101‑1 (MQ=255)
                                                                                 tCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGcc                >  1:6145275/1‑101 (MQ=255)
                                                                                 tCATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGcc                <  1:6144802/101‑1 (MQ=255)
                                                                                  cATCGCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCg               >  2:6145277/1‑101 (MQ=255)
                                                                                    tCGCCCTGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGAACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCcat                         >  1:6145279/1‑89 (MQ=255)
                                                                                      gCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCt           <  2:6145028/101‑1 (MQ=255)
                                                                                      gCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCt           >  2:6145282/1‑101 (MQ=255)
                                                                                      gCCCTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCt           <  1:6145145/101‑1 (MQ=255)
                                                                                        ccTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCgg              <  2:6144681/96‑1 (MQ=255)
                                                                                         cTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGtt        <  2:6144832/101‑1 (MQ=255)
                                                                                         cTGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGtt        >  1:6145286/1‑101 (MQ=255)
                                                                                          tGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTc       >  2:6145288/1‑101 (MQ=255)
                                                                                          tGGGGGGCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTc       <  1:6145247/101‑1 (MQ=255)
                                                                                           ggggggCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTTCCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCg      <  2:6144743/101‑1 (MQ=255)
                                                                                           ggggggCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCgg              <  2:6144919/93‑1 (MQ=255)
                                                                                            gggggCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGt     >  1:6145291/1‑101 (MQ=255)
                                                                                              gggCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCt   <  1:6144996/101‑1 (MQ=255)
                                                                                              gggCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCt   <  1:6144835/101‑1 (MQ=255)
                                                                                               ggCGCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCtt  >  2:3210414/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
AGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTT  >  NC_000913/3980861‑3981056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑