BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	371,924	C→T	A229V (GCG→GTG)	mhpC →	2‑hydroxy‑6‑ketonona‑2,4‑dienedioate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	371,924	0	C	T	100.0%	1244.7 / NA	296	A229V (GCG→GTG)	mhpC	2‑hydroxy‑6‑ketonona‑2,4‑dienedioate hydrolase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (179/117); total (179/117)

Only 100 of 297 total aligned reads displayed.

GCTGTCGCGCCGCGATCACCTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGCGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATC  >  NC_000913/371826‑372021

 cTGTCGCGCCGCGATCACCTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCa                                                                                                >  2:583039/1‑101 (MQ=255)

     cgcgCCGCGATCACCTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAAcc                                                                                            >  2:583044/1‑101 (MQ=255)

      gcgcCGCGATCACCTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAAccc                                                                                           >  1:583046/1‑101 (MQ=255)

        gccgcGATCACCTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTg                                                                                         >  2:583049/1‑101 (MQ=255)

          cgcgATCACCTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGAt                                                                                       >  1:583055/1‑101 (MQ=255)

          cgcgATCACCTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGAt                                                                                       >  2:583054/1‑101 (MQ=255)

           gcgATCACCTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAAccc                                                                                           <  1:582641/96‑1 (MQ=255)

           gcgATCACCTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGAtt                                                                                      >  1:583062/1‑101 (MQ=255)

           gcgATCACCTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGAtt                                                                                      >  2:583060/1‑101 (MQ=255)

            cgATCACCTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTg                                                                                     <  2:582704/101‑1 (MQ=255)

             gATCACCTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGt                                                                                    >  2:583065/1‑101 (MQ=255)

              aTCACCTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTc                                                                                   >  1:583068/1‑101 (MQ=255)

                cACCTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTg                                                                                 <  1:582653/101‑1 (MQ=255)

                cACCTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTg                                                                                 <  2:582969/101‑1 (MQ=255)

                cACCTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTg                                                                                 >  1:583072/1‑101 (MQ=255)

                  ccTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTggg                                                                               <  1:582731/101‑1 (MQ=255)

                  ccTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTggg                                                                               <  2:582591/101‑1 (MQ=255)

                   cTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTgggg                                                                              <  2:582522/101‑1 (MQ=255)

                   cTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTgggg                                                                              >  2:583077/1‑101 (MQ=255)

                   cTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTgggg                                                                              >  1:583076/1‑101 (MQ=255)

                      gAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGgcg                                                                           >  1:583083/1‑101 (MQ=255)

                       aaaaCTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGgcgc                                                                          >  1:583088/1‑101 (MQ=255)

                       aaaaCTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGgcgc                                                                          >  2:583085/1‑101 (MQ=255)

                         aaCTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCaa                                                                        >  1:583092/1‑101 (MQ=255)

                         aaCTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCaa                                                                        >  2:583094/1‑101 (MQ=255)

                          aCTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAAc                                                                       <  1:582553/101‑1 (MQ=255)

                           cTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACg                                                                      <  1:582847/101‑1 (MQ=255)

                            ttCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGa                                                                     >  2:583097/1‑101 (MQ=255)

                             tCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGAc                                                                    <  2:582500/101‑1 (MQ=255)

                              cGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGAcc                                                                   >  1:583101/1‑101 (MQ=255)

                               gTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCg                                                                  >  2:583103/1‑101 (MQ=255)

                                 tAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTgggg                                                                              >  2:583109/1‑87 (MQ=255)

                                 tAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCt                                                                >  1:583106/1‑101 (MQ=255)

                                  aaGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCtt                                                               <  1:582576/101‑1 (MQ=255)

                                    gagCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTtg                                                             >  1:583113/1‑101 (MQ=255)

                                     agCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTtgt                                                            >  2:583115/1‑101 (MQ=255)

                                      gCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTtgtg                                                           >  1:583118/1‑101 (MQ=255)

                                      gCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTtgtg                                                           <  2:582760/101‑1 (MQ=255)

                                        cTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGcc                                                         >  2:583121/1‑101 (MQ=255)

                                        cTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGcc                                                         <  2:582783/101‑1 (MQ=255)

                                         tGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCg                                                        >  1:583123/1‑101 (MQ=255)

                                           gAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCgat                                                      <  2:582804/101‑1 (MQ=255)

                                           gAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCgat                                                      <  2:582832/101‑1 (MQ=255)

                                            aaGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCgatg                                                     >  2:583128/1‑101 (MQ=255)

                                             aGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCgatgg                                                    >  1:583131/1‑101 (MQ=255)

                                              gCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCgatgga                                                   <  2:582911/101‑1 (MQ=255)

                                              gCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCgatgga                                                   >  1:583134/1‑101 (MQ=255)

                                                tAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCgatggatg                                                 <  2:582844/101‑1 (MQ=255)

                                                 aaTCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGc                                                >  1:583139/1‑101 (MQ=255)

                                                  aTCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCg                                               <  1:582443/101‑1 (MQ=255)

                                                  aTCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCg                                               >  2:583143/1‑101 (MQ=255)

                                                  aTCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCg                                               >  1:583142/1‑101 (MQ=255)

                                                   tCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCgg                                              <  1:582510/101‑1 (MQ=255)

                                                   tCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCgg                                              <  1:582807/101‑1 (MQ=255)

                                                    ccGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCggg                                             <  1:582881/101‑1 (MQ=255)

                                                      gAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCaatggatg                                                 >  1:583148/1‑95 (MQ=255)

                                                       aaaCAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGgtct                                          <  2:582581/101‑1 (MQ=255)

                                                        aaCAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGgtctg                                         <  1:582694/101‑1 (MQ=255)

                                                        aaCAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGgtctg                                         >  1:583151/1‑101 (MQ=255)

                                                         aCAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGgtctgc                                        <  1:582737/101‑1 (MQ=255)

                                                          cAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGgtctgcg                                       >  2:583153/1‑101 (MQ=255)

                                                             ttCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGgtctgcgtct                                    <  1:582682/101‑1 (MQ=255)

                                                              tCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGgtctgcgtctg                                   <  1:582784/101‑1 (MQ=255)

                                                              tCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGgtctgcgtctg                                   >  1:583156/1‑101 (MQ=255)

                                                                 cGGATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTctgctg                                >  2:583160/1‑101 (MQ=255)

                                                                   gATTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTc                              >  1:583162/1‑101 (MQ=255)

                                                                    aTTTTGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTcc                             >  2:583165/1‑101 (MQ=255)

                                                                     ttttGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCg                            >  1:583167/1‑101 (MQ=255)

                                                                     ttttGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCg                            <  2:582772/101‑1 (MQ=255)

                                                                      tttGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCgg                           <  1:582749/101‑1 (MQ=255)

                                                                       ttGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTc                              >  1:583172/1‑97 (MQ=255)

                                                                       ttGGCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGc                          <  1:582614/101‑1 (MQ=255)

                                                                          gCCCACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCAtt                       <  2:582717/101‑1 (MQ=255)

                                                                           cccACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTg                      >  2:583176/1‑101 (MQ=255)

                                                                           cccACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTg                      >  1:583175/1‑101 (MQ=255)

                                                                            ccACGTCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGc                     >  1:583179/1‑101 (MQ=255)

                                                                                 tCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTggggg                                                                             <  2:582736/40‑1 (MQ=255)

                                                                                 tCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTc                              <  2:582562/87‑1 (MQ=255)

                                                                                 tCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGtt                <  1:582777/101‑1 (MQ=255)

                                                                                 tCTGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGtt                >  2:583183/1‑101 (MQ=255)

                                                                                   tGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCt              >  2:583188/1‑101 (MQ=255)

                                                                                   tGGCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCt              <  1:582713/101‑1 (MQ=255)

                                                                                   aggCGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCt              >  1:583186/2‑101 (MQ=255)

                                                                                      cGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGaa           >  1:583193/1‑101 (MQ=255)

                                                                                      cGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGaa           >  2:583194/1‑101 (MQ=255)

                                                                                      cGGAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGaa           >  2:583198/1‑101 (MQ=255)

                                                                                       ggAAATCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAAc          <  2:582668/101‑1 (MQ=255)

                                                                                         aaaTCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGcc                    <  2:582647/89‑1 (MQ=255)

                                                                                         aaaTCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTg        >  1:583201/1‑101 (MQ=255)

                                                                                         aaaTCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTg        >  2:583205/1‑101 (MQ=255)

                                                                                          aaTCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGc       >  2:583210/1‑101 (MQ=255)

                                                                                          aaTCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGc       >  1:583207/1‑101 (MQ=255)

                                                                                            tCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCat     <  2:582727/101‑1 (MQ=255)

                                                                                            tCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCat     >  1:583214/1‑101 (MQ=255)

                                                                                            tCAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCat     <  1:583030/101‑1 (MQ=255)

                                                                                             cAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCata    <  2:582750/101‑1 (MQ=255)

                                                                                             cAAAGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCata    >  2:583216/1‑101 (MQ=255)

                                                                                              aaaGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCatat   <  1:582913/101‑1 (MQ=255)

                                                                                              aaaGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCatat   >  2:583219/1‑101 (MQ=255)

                                                                                               aaGTGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATc  <  1:582645/101‑1 (MQ=255)

GCTGTCGCGCCGCGATCACCTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAACAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGCGCAAACCCTGATTGTCTGGGGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATC  >  NC_000913/371826‑372021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`