BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	166,503	A→G	T592A (ACC→GCC)	mrcB →	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	166,503	0	A	G	100.0%	1016.6 / NA	246	T592A (ACC→GCC)	mrcB	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (108/138); total (108/138)

Only 100 of 247 total aligned reads displayed.

AAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGACCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACGCCAATCGAAGTGGCGCAGGC  >  NC_000913/166405‑166600

  aGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTg                                                                                               >  2:262988/1‑101 (MQ=255)

  aGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTg                                                                                               >  1:262987/1‑101 (MQ=255)

   gCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTgg                                                                                              <  2:262168/101‑1 (MQ=255)

    cGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGa                                                                                             <  1:262659/101‑1 (MQ=255)

    cGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGa                                                                                             >  2:262990/1‑101 (MQ=255)

      gCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGTATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGAtt                                                                                           <  2:262864/101‑1 (MQ=255)

      gCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGAtt                                                                                           >  1:262992/1‑101 (MQ=255)

       cAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTa                                                                                          <  1:262445/101‑1 (MQ=255)

        agagTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTaa                                                                                         <  1:262488/101‑1 (MQ=255)

        agagTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTaa                                                                                         <  1:262566/101‑1 (MQ=255)

          agTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAAc                                                                                       >  2:262998/1‑101 (MQ=255)

          agTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAAc                                                                                       >  1:262997/1‑101 (MQ=255)

           gTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACt                                                                                      <  1:262207/101‑1 (MQ=255)

             gATGCTTGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTgg                                                                                    >  1:263002/1‑101 (MQ=255)

              aTGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTggg                                                                                   <  2:262576/101‑1 (MQ=255)

              aTGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTggg                                                                                   >  2:263003/1‑101 (MQ=255)

               tGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGc                                                                                  <  1:262326/101‑1 (MQ=255)

                 cTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGt                                                                                <  2:262317/101‑1 (MQ=255)

                 cTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGt                                                                                >  2:263007/1‑101 (MQ=255)

                 cTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGt                                                                                <  2:262530/101‑1 (MQ=255)

                  tggtggATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTa                                                                               >  2:263010/1‑101 (MQ=255)

                   ggtggATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAAc                                                                                       >  1:263011/1‑92 (MQ=255)

                        aTGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGaaa                                                                         <  1:262539/101‑1 (MQ=255)

                        aTGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGaaa                                                                         >  2:263015/1‑101 (MQ=255)

                         tGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGc                                                                                  >  2:263018/1‑91 (MQ=255)

                         tGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAg                                                                        >  1:263016/1‑101 (MQ=255)

                          gCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGa                                                                       <  1:262675/101‑1 (MQ=255)

                           cGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGAt                                                                      <  1:262555/101‑1 (MQ=255)

                            gTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATc                                                                     <  1:262583/101‑1 (MQ=255)

                            gTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATc                                                                     <  2:262409/101‑1 (MQ=255)

                             ttGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCa                                                                    <  2:262630/101‑1 (MQ=255)

                             ttGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCa                                                                    >  2:263023/1‑101 (MQ=255)

                                aCCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGtt                                                                 <  1:262950/101‑1 (MQ=255)

                                aCCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGtt                                                                 <  1:262604/101‑1 (MQ=255)

                                aCCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGtt                                                                 >  2:263025/1‑101 (MQ=255)

                                    gTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCAt                                                             <  2:262702/101‑1 (MQ=255)

                                    gTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCAt                                                             <  1:262683/101‑1 (MQ=255)

                                    gTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCAt                                                             <  1:262648/101‑1 (MQ=255)

                                     ttCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATc                                                            <  2:262777/101‑1 (MQ=255)

                                     ttCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATc                                                            >  2:263031/1‑101 (MQ=255)

                                      tCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATcc                                                           <  1:262629/101‑1 (MQ=255)

                                      tCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATcc                                                           >  1:263033/1‑101 (MQ=255)

                                       cGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCg                                                          >  2:263035/1‑101 (MQ=255)

                                         aTGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGt                                                        >  2:263038/1‑101 (MQ=255)

                                         aTGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGt                                                        <  2:262536/101‑1 (MQ=255)

                                         aTGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGt                                                        >  1:263039/1‑101 (MQ=255)

                                           gAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTc                                                      >  1:263043/1‑101 (MQ=255)

                                           gAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTc                                                      <  2:262660/101‑1 (MQ=255)

                                            aaCGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTcc                                                     <  1:262609/101‑1 (MQ=255)

                                            aaCGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTcc                                                     <  1:262710/101‑1 (MQ=255)

                                             aCGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCg                                                    <  2:262828/101‑1 (MQ=255)

                                             aCGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCg                                                    <  1:262447/101‑1 (MQ=255)

                                                tGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCa                                                 >  2:263047/1‑101 (MQ=255)

                                                tGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCa                                                 <  2:262761/101‑1 (MQ=255)

                                                 gCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTcc                                                     <  2:262742/96‑1 (MQ=255)

                                                 gCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCaa                                                >  1:263049/1‑101 (MQ=255)

                                                   cGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAAtg                                              >  2:263051/1‑101 (MQ=255)

                                                     aCGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAAtgct                                            <  1:262619/101‑1 (MQ=255)

                                                      cGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAAtgctg                                           <  2:262592/101‑1 (MQ=255)

                                                      cGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAAtgctg                                           >  2:263055/1‑101 (MQ=255)

                                                       ggTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAAtgctgc                                          >  2:263058/1‑101 (MQ=255)

                                                        gTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATgctgct                                         <  2:262643/101‑1 (MQ=255)

                                                            aTCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTgggg                                     <  1:262579/101‑1 (MQ=255)

                                                             tCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTggggg                                    >  1:263061/1‑101 (MQ=255)

                                                              cTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGc                                   <  2:262611/101‑1 (MQ=255)

                                                              cTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATGAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGc                                   >  2:263062/1‑101 (MQ=255)

                                                               tGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCg                                  <  2:262731/101‑1 (MQ=255)

                                                               tGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCg                                  <  1:262638/101‑1 (MQ=255)

                                                               tGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCg                                  <  2:262637/101‑1 (MQ=255)

                                                                ggggATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGt                                 <  1:262749/101‑1 (MQ=255)

                                                                ggggATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGt                                 >  2:263064/1‑101 (MQ=255)

                                                                 gggATGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGtt                                <  1:262601/101‑1 (MQ=255)

                                                                     tGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGa                              <  2:262621/99‑1 (MQ=255)

                                                                     tGGCGCTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAAc                            <  2:262677/101‑1 (MQ=255)

                                                                         gcTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACtt                          <  2:262484/99‑1 (MQ=255)

                                                                         gcTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTaa                        <  1:262548/101‑1 (MQ=255)

                                                                         gcTGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTaa                        >  1:263068/1‑101 (MQ=255)

                                                                           tGGGGCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACg                      <  2:262457/101‑1 (MQ=255)

                                                                            ggggCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACGc                     >  2:263071/1‑101 (MQ=255)

                                                                            ggggCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACGc                     >  1:263072/1‑101 (MQ=255)

                                                                               gCTGCCTGCGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACGCCaa                  <  2:262691/101‑1 (MQ=255)

                                                                                ctgcctgcGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACGCCAAt                 >  2:263075/1‑101 (MQ=255)

                                                                                ctgcctgcGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACGCCAAt                 >  1:263076/1‑101 (MQ=255)

                                                                                 tgcctgcGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACGCCAATc                <  1:262666/101‑1 (MQ=255)

                                                                                  gcctgcGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACGCCAATCg               >  2:263079/1‑101 (MQ=255)

                                                                                  gcctgcGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACGCCAATCg               <  1:262511/101‑1 (MQ=255)

                                                                                   cctgcGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACGCCAATCGa              <  2:262517/101‑1 (MQ=255)

                                                                                   cctgcGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACGCCAATCGa              >  2:263081/1‑101 (MQ=255)

                                                                                    ctgcGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACGCCAATCGaa             >  2:263087/1‑101 (MQ=255)

                                                                                    ctgcGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACGCCAATCGaa             >  1:263086/1‑101 (MQ=255)

                                                                                    ctgcGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACGCCAATCGaa             <  2:262651/101‑1 (MQ=255)

                                                                                     tgcGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACGCCAATCGAAg            >  1:263090/1‑101 (MQ=255)

                                                                                     tgcGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACGCCAATCGAAg            <  2:262706/101‑1 (MQ=255)

                                                                                      gcGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGtt                                                                 <  1:262374/47‑1 (MQ=255)

                                                                                      gcGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACGCCAATCGAAGt           <  2:262448/101‑1 (MQ=255)

                                                                                       cGGTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACGCCAATCGAAGTg          >  2:263093/1‑101 (MQ=255)

                                                                                        ggTTACGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACGCCAATc                <  2:262562/94‑1 (MQ=255)

                                                                                            aCGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACGCCAATCGAAGTGGCGCa     <  1:263027/101‑1 (MQ=255)

                                                                                             cGGAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACGCCAATCGAAGTGGCGCAg    >  1:263096/1‑101 (MQ=255)

                                                                                              ggAGGCCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACGCCAATCGAAGTGGCGCAgg   <  1:262714/101‑1 (MQ=255)

AAAGCGGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCCTGCGGTTACGGAGACCTGGATTAAACTGGGCGTACCGAAAGATCAGTTGCATCCGGTTCCGGCAATGCTGCTGGGGGCGTTGAACTTAACGCCAATCGAAGTGGCGCAGGC  >  NC_000913/166405‑166600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`