BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	192,297	C→T	A148V (GCA→GTA)	pyrH →	UMP kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	192,297	0	C	T	100.0%	1393.0 / NA	324	A148V (GCA→GTA)	pyrH	UMP kinase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (151/173); total (151/173)

Only 100 of 333 total aligned reads displayed.

CAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACC  >  NC_000913/192199‑192394

cagctacagctGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTcagtag                                                                                                 <  2:301591/101‑1 (MQ=255)

   ctacagctGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCtt                                                                                              <  1:301719/101‑1 (MQ=255)

    tacagctGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTg                                                                                             >  1:302200/1‑101 (MQ=255)

    tacagctGGGCAGAAGCTATCAGCCTGTTACGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTg                                                                                             <  2:301503/101‑1 (MQ=255)

     acagctGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGGGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGc                                                                                            >  2:302202/1‑101 (MQ=255)

      cagctGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGcc                                                                                           <  1:301696/101‑1 (MQ=255)

       agctGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCt                                                                                          <  2:301822/101‑1 (MQ=255)

       agctGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCt                                                                                          >  1:302207/1‑101 (MQ=255)

        gctGGGCAGAAGCTATCAGCCTGTTGCGAAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTg                                                                                         >  2:302208/1‑101 (MQ=255)

           gggCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGc                                                                                        >  2:302213/1‑99 (MQ=255)

           gggCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGt                                                                                      <  1:301932/101‑1 (MQ=255)

           gggCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGt                                                                                      <  2:301847/101‑1 (MQ=255)

             gCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTgg                                                                                    >  1:302216/1‑101 (MQ=255)

              cAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGt                                                                                   >  2:302220/1‑101 (MQ=255)

               aGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGt                                                                                      >  2:302228/1‑97 (MQ=255)

               aGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTa                                                                                  >  1:302224/1‑101 (MQ=255)

                gAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTAt                                                                                 >  1:302229/1‑101 (MQ=255)

                  aGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCg                                                                               >  2:302233/1‑101 (MQ=255)

                   gCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGa                                                                              >  1:302235/1‑101 (MQ=255)

                   gCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGa                                                                              <  1:301380/101‑1 (MQ=255)

                      aTCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGaaa                                                                            <  2:301557/100‑1 (MQ=255)

                        cAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTg                                                                         <  2:301739/101‑1 (MQ=255)

                         aGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGa                                                                        <  2:301771/101‑1 (MQ=255)

                         aGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGa                                                                        <  1:301773/101‑1 (MQ=255)

                          gCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGaa                                                                       <  1:301865/101‑1 (MQ=255)

                          gCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGaa                                                                       >  1:302242/1‑101 (MQ=255)

                           ccTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAg                                                                      <  2:302178/101‑1 (MQ=255)

                           ccTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAg                                                                      <  1:301747/101‑1 (MQ=255)

                            cTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGc                                                                     >  2:302244/1‑101 (MQ=255)

                             tGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGcc                                                                    <  2:301940/101‑1 (MQ=255)

                              gTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTg                                                                         <  2:301708/95‑1 (MQ=255)

                              gTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCg                                                                   <  2:301765/101‑1 (MQ=255)

                              gTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCg                                                                   <  2:301786/101‑1 (MQ=255)

                                tGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCg                                                                   >  1:302248/1‑99 (MQ=255)

                                 gcgcAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGAtg                                                                <  1:301783/101‑1 (MQ=255)

                                  cgcAACAACAGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGAtgt                                                               <  1:301823/101‑1 (MQ=255)

                                   gcAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGAtgtg                                                              >  2:302252/1‑101 (MQ=255)

                                   gcAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGAtgtg                                                              <  1:301646/101‑1 (MQ=255)

                                    caacaaCCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATgtgg                                                             >  1:302254/1‑101 (MQ=255)

                                     aacaacCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATgtggt                                                            >  2:302258/1‑101 (MQ=255)

                                       caacCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGc                                                          >  2:302262/1‑101 (MQ=255)

                                       caacCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGc                                                          <  1:301827/101‑1 (MQ=255)

                                        aacCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGAtg                                                                <  1:301807/94‑1 (MQ=255)

                                        aacCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCt                                                         <  1:301961/101‑1 (MQ=255)

                                        aacCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCt                                                         >  2:302266/1‑101 (MQ=255)

                                        aacCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCt                                                         <  1:301658/101‑1 (MQ=255)

                                         acCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTg                                                        >  1:302268/1‑101 (MQ=255)

                                         acCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTg                                                        <  2:301726/101‑1 (MQ=255)

                                               tggtgATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAg                                                    <  2:301667/99‑1 (MQ=255)

                                               tggtgATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCa                                                  >  2:302272/1‑101 (MQ=255)

                                                  tgATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAAcc                                               <  2:301819/101‑1 (MQ=255)

                                                   gATCCTCTCCGCAGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCa                                              >  1:302274/1‑101 (MQ=255)

                                                    aTCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCaa                                             <  2:301916/101‑1 (MQ=255)

                                                      ccTCTCCGCCTGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAg                                           >  1:302279/1‑101 (MQ=255)

                                                      ccTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAg                                           <  2:301944/101‑1 (MQ=255)

                                                        tctcCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGtt                                         >  2:302281/1‑101 (MQ=255)

                                                        tctcCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGtt                                         <  1:301548/101‑1 (MQ=255)

                                                        tctcCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGtt                                         <  2:301879/101‑1 (MQ=255)

                                                        tctcCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGtt                                         <  1:301736/101‑1 (MQ=255)

                                                          tcCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGa                                       >  1:302285/1‑101 (MQ=255)

                                                            cgccgGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACg                                     >  2:302289/1‑101 (MQ=255)

                                                             gccgGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACgg                                    >  1:302291/1‑101 (MQ=255)

                                                              ccgGTACAGGCAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAAtt                                                                          >  2:302295/1‑62 (MQ=255)

                                                               cgGTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCg                                  <  2:302015/101‑1 (MQ=255)

                                                                 gTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCgtg                                >  1:302302/1‑101 (MQ=255)

                                                                 gTACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCgtg                                >  2:302300/1‑101 (MQ=255)

                                                                  tACAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCgtgt                               <  2:301854/101‑1 (MQ=255)

                                                                    cAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGttt                             >  2:302304/1‑101 (MQ=255)

                                                                    cAGGTAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGttt                             <  2:301700/101‑1 (MQ=255)

                                                                        tAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCg                         >  2:302310/1‑101 (MQ=255)

                                                                        tAACCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCg                         >  1:302313/1‑101 (MQ=255)

                                                                         aaCCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGt                                                                                   >  2:302315/1‑42 (MQ=255)

                                                                         aaCCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGc                        <  1:301754/101‑1 (MQ=255)

                                                                         aaCCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGc                        <  2:301810/101‑1 (MQ=255)

                                                                         aaCCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTCGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGc                        <  1:301971/101‑1 (MQ=255)

                                                                          aCCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCt                       <  2:301970/101‑1 (MQ=255)

                                                                          aCCCGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCt                       <  1:301885/101‑1 (MQ=255)

                                                                           cccGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTg                      >  1:302319/1‑101 (MQ=255)

                                                                            ccGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGa                     <  2:301687/101‑1 (MQ=255)

                                                                            ccGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGa                     <  1:301793/101‑1 (MQ=255)

                                                                             cGTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGAt                    >  2:302323/1‑101 (MQ=255)

                                                                              gTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATc                   >  1:302324/1‑101 (MQ=255)

                                                                              gTTCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATc                   <  1:301923/101‑1 (MQ=255)

                                                                               ttCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGc                        <  2:301751/95‑1 (MQ=255)

                                                                               ttCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATcc                  >  2:302329/1‑101 (MQ=255)

                                                                                tCTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCg                 <  1:302035/101‑1 (MQ=255)

                                                                                 cTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCgg                <  1:301895/101‑1 (MQ=255)

                                                                                 cTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCgg                >  1:302333/1‑101 (MQ=255)

                                                                                 cTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCgg                <  2:301840/101‑1 (MQ=255)

                                                                                 cTTTACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCgg                >  2:302335/1‑101 (MQ=255)

                                                                                  tttACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGc               <  1:301843/101‑1 (MQ=255)

                                                                                   ttACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCg              <  2:302063/101‑1 (MQ=255)

                                                                                    tACCACCGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGa             >  1:302338/1‑101 (MQ=255)

                                                                                     accaccGCCTCAGTAGCTTGCCTGCGTGGTATCGAAAtt                                                                          >  2:302343/1‑39 (MQ=255)

                                                                                     accaccGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGaa            <  2:301897/101‑1 (MQ=255)

                                                                                       caccGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAg          >  1:302344/1‑101 (MQ=255)

                                                                                         ccGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGAt        >  1:302349/1‑101 (MQ=255)

                                                                                         ccGACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGAt        >  2:302351/1‑101 (MQ=255)

                                                                                           gACTCAGTAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATcc      <  1:302013/101‑1 (MQ=255)

                                                                                               cagtagCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCg                         >  1:302357/1‑78 (MQ=255)

CAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACC  >  NC_000913/192199‑192394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`