BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,262,915	G→A	P194L (CCG→CTG)	fruB ←	fructose‑specific PTS multiphosphoryl transfer protein FruB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,262,915	0	G	A	100.0%	1091.3 / NA	248	P194L (CCG→CTG)	fruB	fructose‑specific PTS multiphosphoryl transfer protein FruB
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (139/109); total (139/109)

Only 100 of 249 total aligned reads displayed.

CCACATCAAAAGCATTTGCCGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCGGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCT  >  NC_000913/2262817‑2263012

ccACATCAAAAGCATTTGCCGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAgt                                                                                                 <  1:3437298/101‑1 (MQ=255)

ccACATCAAAAGCATTTGCCGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAgt                                                                                                 <  2:3437399/101‑1 (MQ=255)

  acaTCAAAAGCATTTGCCGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAgttg                                                                                               <  2:3437473/101‑1 (MQ=255)

   caTCAAAAGCATTTGCCGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAgttgt                                                                                              <  2:3437187/101‑1 (MQ=255)

   caTCAAAAGCATTTGCCGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAgttgt                                                                                              >  1:3437887/1‑101 (MQ=255)

   caTCAAAAGCATTTGCCGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAgttgt                                                                                              >  2:3437886/1‑101 (MQ=255)

   caTCAAAAGCATTTGCCGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAgttgt                                                                                              >  2:3437889/1‑101 (MQ=255)

     tCAAAAGCATTTGCCGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTc                                                                                            >  2:3437892/1‑101 (MQ=255)

       aaaaGCATTTGCCGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCAt                                                                                          <  2:3437507/101‑1 (MQ=255)

         aaGCATTTGCCGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTg                                                                                        <  2:3437282/101‑1 (MQ=255)

         aaGCATTTGCCGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTg                                                                                        >  1:3437896/1‑101 (MQ=255)

         aaGCATTTGCCGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTg                                                                                        <  2:3437431/101‑1 (MQ=255)

         aaGCATTTGCCGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTg                                                                                        <  2:3437460/101‑1 (MQ=255)

           gCATTTGCCGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGAt                                                                                      <  1:3437206/101‑1 (MQ=255)

            cATTTGCCGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATg                                                                                     >  2:3437897/1‑101 (MQ=255)

            cATTTGCCGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATg                                                                                     <  2:3437356/101‑1 (MQ=255)

                 gCCGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCttt                                                                                >  2:3437902/1‑101 (MQ=255)

                 gCCGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCttt                                                                                <  2:3437696/101‑1 (MQ=255)

                  ccGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTg                                                                               <  2:3437494/101‑1 (MQ=255)

                  ccGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTg                                                                               <  2:3437437/101‑1 (MQ=255)

                  ccGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTg                                                                               >  1:3437903/1‑101 (MQ=255)

                  ccGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTg                                                                               <  2:3437619/101‑1 (MQ=255)

                    gCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGt                                                                             >  1:3437906/1‑101 (MQ=255)

                     cACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTg                                                                            >  2:3437912/1‑101 (MQ=255)

                     cACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTg                                                                            <  2:3437766/101‑1 (MQ=255)

                     cACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTg                                                                            >  2:3437909/1‑101 (MQ=255)

                      aCGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGa                                                                           <  2:3437638/101‑1 (MQ=255)

                      aCGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGa                                                                           >  1:3437913/1‑101 (MQ=255)

                      aCGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGa                                                                           <  2:3437326/101‑1 (MQ=255)

                       cGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGAc                                                                          >  1:3437916/1‑101 (MQ=255)

                       cGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGAc                                                                          <  2:3437590/101‑1 (MQ=255)

                        ggCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACg                                                                         <  1:3437381/101‑1 (MQ=255)

                         gCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGa                                                                        <  1:3437254/101‑1 (MQ=255)

                           ttACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTGAGCAGTTGTTCATTGATGGCTTTGGTGACGaaa                                                                      <  1:3437634/101‑1 (MQ=255)

                           ttACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGaaa                                                                      >  2:3437919/1‑101 (MQ=255)

                            tACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAg                                                                     <  1:3437581/101‑1 (MQ=255)

                            tACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAg                                                                     >  2:3437923/1‑101 (MQ=255)

                            tACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAg                                                                     >  1:3437920/1‑101 (MQ=255)

                              ccGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTg                                                                   <  1:3437355/101‑1 (MQ=255)

                               cGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTgg                                                                  >  2:3437926/1‑101 (MQ=255)

                               cGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTgg                                                                  >  1:3437927/1‑101 (MQ=255)

                               cGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTg                                                                   <  1:3437610/100‑1 (MQ=255)

                                 cAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCg                                                                <  2:3437532/101‑1 (MQ=255)

                                  aaTCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGt                                                               <  2:3437566/101‑1 (MQ=255)

                                  aaTCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGt                                                               <  2:3437653/101‑1 (MQ=255)

                                  aaTCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGt                                                               >  1:3437931/1‑101 (MQ=255)

                                   aTCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTc                                                              >  1:3437933/1‑101 (MQ=255)

                                   aTCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTc                                                              <  1:3437447/101‑1 (MQ=255)

                                    tCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCa                                                             <  1:3437467/101‑1 (MQ=255)

                                    tCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCa                                                             <  1:3437568/101‑1 (MQ=255)

                                    tCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCa                                                             <  1:3437336/101‑1 (MQ=255)

                                      gcgcTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAAc                                                           >  2:3437937/1‑101 (MQ=255)

                                        gcTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTg                                                         <  2:3437255/101‑1 (MQ=255)

                                        gcTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTg                                                         >  1:3437938/1‑101 (MQ=255)

                                        gcTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTg                                                         <  1:3437458/101‑1 (MQ=255)

                                         cTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGc                                                        <  1:3437510/101‑1 (MQ=255)

                                          tACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGcc                                                       >  2:3437942/1‑101 (MQ=255)

                                          tACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGcc                                                       >  1:3437941/1‑101 (MQ=255)

                                            cGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGcccc                                                     <  1:3437489/101‑1 (MQ=255)

                                             gCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGccccc                                                    >  2:3437946/1‑101 (MQ=255)

                                               aGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGc                                                  >  2:3437951/1‑101 (MQ=255)

                                               aGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGc                                                  >  2:3437948/1‑101 (MQ=255)

                                                gATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGcc                                                 <  2:3437539/101‑1 (MQ=255)

                                                  ttGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCctc                                               >  2:3437954/1‑101 (MQ=255)

                                                   tGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCctct                                              >  1:3437955/1‑101 (MQ=255)

                                                    gCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCct                                                <  1:3437483/98‑1 (MQ=255)

                                                        tCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAg                                         >  1:3437958/1‑101 (MQ=255)

                                                          ggCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGAc                                       <  1:3437870/101‑1 (MQ=255)

                                                          ggCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGAc                                       >  1:3437961/1‑101 (MQ=255)

                                                           gcgcTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTg                                                                   <  1:3437655/72‑1 (MQ=255)

                                                            cgcTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGAcgc                                     >  1:3437963/1‑101 (MQ=255)

                                                              cTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACgcgc                                   >  1:3437967/1‑101 (MQ=255)

                                                               tATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCa                                  <  1:3437730/101‑1 (MQ=255)

                                                               tATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCa                                  >  2:3437968/1‑101 (MQ=255)

                                                                aTCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAg                                 <  1:3437409/101‑1 (MQ=255)

                                                                 tCGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGc                                >  2:3437970/1‑101 (MQ=255)

                                                                  cGCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGAcg                                      >  2:3437974/1‑94 (MQ=255)

                                                                   gCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGt                              >  1:3437976/1‑101 (MQ=255)

                                                                   gCTCAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGt                              >  2:3437978/1‑101 (MQ=255)

                                                                      cAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCa                                  >  2:3437983/1‑94 (MQ=255)

                                                                      cAGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCa                           >  1:3437982/1‑101 (MQ=255)

                                                                       aGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAg                          >  1:3437987/1‑101 (MQ=255)

                                                                       aGCCAGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAg                          >  2:3437985/1‑101 (MQ=255)

                                                                           aGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGcc                      >  1:3437992/1‑101 (MQ=255)

                                                                           aGATACCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGcc                      >  2:3437993/1‑101 (MQ=255)

                                                                               aCCCTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGaa                  >  2:3437996/1‑101 (MQ=255)

                                                                                 ccTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAaga                >  2:3437999/1‑101 (MQ=255)

                                                                                 ccTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAaga                >  2:3438001/1‑101 (MQ=255)

                                                                                  cTGTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAagag               >  2:3438004/1‑101 (MQ=255)

                                                                                    gTCCGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTc             >  1:3438005/1‑101 (MQ=255)

                                                                                      ccGAGATTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTcag           >  2:3438008/1‑101 (MQ=255)

                                                                                        gagaTTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTcagca         >  2:3438011/1‑101 (MQ=255)

                                                                                           aTTCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGAt      <  1:3437545/101‑1 (MQ=255)

                                                                                            ttCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATc     <  2:3437606/101‑1 (MQ=255)

                                                                                            ttCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATc     >  2:3438013/1‑101 (MQ=255)

                                                                                            ttCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATc     <  1:3437451/101‑1 (MQ=255)

                                                                                            ttCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATc     <  1:3437677/101‑1 (MQ=255)

                                                                                             tCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCa                           >  2:3438018/1‑78 (MQ=255)

                                                                                             tCAGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCg    >  1:3438016/1‑101 (MQ=255)

                                                                                               aGCAGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCt  >  2:3438020/1‑101 (MQ=255)

CCACATCAAAAGCATTTGCCGCACGGCTTACCGCAATCGCGCTACGCAGATTGCCTTCGGCGCTATCGCTCAGCCAGATACCCTGTCCGAGATTCAGCGGTTGTTCATTGATGGCTTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCT  >  NC_000913/2262817‑2263012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`