BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,302,729	T→C	M9V (ATG→GTG)	napA ←	periplasmic nitrate reductase subunit NapA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,302,729	0	T	C	100.0%	961.2 / NA	230	M9V (ATG→GTG)	napA	periplasmic nitrate reductase subunit NapA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (88/142); total (88/142)

Only 100 of 230 total aligned reads displayed.

CCATTTGATGGCTTCCTGCTGACCAACAACGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCATAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCACGCCCTCTACGTTGCGTA  >  NC_000913/2302631‑2302826

ccATTTGATGGCTTCCTGCTGACCAACAACGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACaa                                                                                                 <  1:3497111/101‑1 (MQ=255)

  aTTTGATGGCTTCCTGCTGACCAACAACGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACaaa                                                                                                >  2:3497409/1‑100 (MQ=255)

   tttGATGGCTTCCTGCTGACCAACAACGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGc                                                                                              <  1:3497224/101‑1 (MQ=255)

    ttGATGGCTTCCTGCTGACCAACAACGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCt                                                                                             >  1:3497414/1‑101 (MQ=255)

    ttGATGGCTTCCTGCTGACCAACAACGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCt                                                                                             >  1:3497412/1‑101 (MQ=255)

      gATGGCTTCCTGCTGACCAACAACGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTac                                                                                           <  1:3496818/101‑1 (MQ=255)

      gATGGCTTCCTGCTGACCAACAACGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTac                                                                                           <  1:3496985/101‑1 (MQ=255)

         ggCTTCCTGCTGACCAACAACGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTacgac                                                                                        >  1:3497421/1‑101 (MQ=255)

             tCCTGCTGACCAACAACGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTacga                                                                                         >  1:3497427/1‑96 (MQ=255)

             tCCTGCTGACCAACAACGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACt                                                                                    <  1:3497187/101‑1 (MQ=255)

             tCCTGCTGACCAACAACGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACt                                                                                    >  1:3497425/1‑101 (MQ=255)

              ccTGCTGACCAACAACGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTg                                                                                   <  1:3497301/101‑1 (MQ=255)

               ctgctgACCAACAACGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGa                                                                                  <  1:3497006/101‑1 (MQ=255)

                tgctgACCAACAACGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAg                                                                                 >  2:3497434/1‑101 (MQ=255)

                  ctgACCAACAACGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGtt                                                                               <  2:3497238/101‑1 (MQ=255)

                   tgACCAACAACGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGttt                                                                              >  1:3497438/1‑101 (MQ=255)

                    gACCAACAACGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTc                                                                             <  1:3497082/101‑1 (MQ=255)

                       caacaaCGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATg                                                                          <  1:3496733/101‑1 (MQ=255)

                        aacaacGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAg                                                                                               <  1:3496981/79‑1 (MQ=255)

                        aacaacGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATgg                                                                         >  1:3497442/1‑101 (MQ=255)

                           aacGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGgtgt                                                                      >  1:3497446/1‑101 (MQ=255)

                             cGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACaaa                                                                                                <  2:3497067/73‑1 (MQ=255)

                             cGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGttt                                                                    <  1:3497096/101‑1 (MQ=255)

                             cGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGttt                                                                    <  1:3497032/101‑1 (MQ=255)

                                cgcgGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCt                                                                 <  1:3496940/101‑1 (MQ=255)

                                cgcgGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCt                                                                 >  1:3497453/1‑101 (MQ=255)

                                 gcgGGCAACCCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCa                                                                            <  1:3497148/89‑1 (MQ=255)

                                     gCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCt                                                            >  1:3497458/1‑101 (MQ=255)

                                        aCGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACgacgac                                                                                     <  1:3496838/73‑1 (MQ=255)

                                        aCGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGc                                                         >  2:3497461/1‑101 (MQ=255)

                                        aCGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGc                                                         <  1:3497203/101‑1 (MQ=255)

                                         cGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTa                                                                                            <  2:3497129/65‑1 (MQ=255)

                                          gCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGtt                                                                     <  2:3496947/87‑1 (MQ=255)

                                            ccGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTg                                                                                   >  1:3497466/1‑71 (MQ=255)

                                            ccGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCtt                                                     >  2:3497465/1‑101 (MQ=255)

                                             cGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTc                                                    <  1:3497161/101‑1 (MQ=255)

                                              ggCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAg                                                                                               <  1:3496958/57‑1 (MQ=255)

                                              ggCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGt                                                                                <  2:3496991/72‑1 (MQ=255)

                                              ggCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCa                                                               <  2:3497271/89‑1 (MQ=255)

                                              ggCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTcc                                                   <  2:3497003/101‑1 (MQ=255)

                                               gCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCct                                                  <  1:3497222/101‑1 (MQ=255)

                                               gCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCct                                                  <  2:3496865/101‑1 (MQ=255)

                                                 aCGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTg                                                          <  1:3497103/91‑1 (MQ=255)

                                                 aCGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCctgc                                                >  1:3497469/1‑101 (MQ=255)

                                                  cGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGt                                                                                <  2:3497118/68‑1 (MQ=255)

                                                   gCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTacgac                                                                                        <  1:3497012/59‑1 (MQ=255)

                                                    cTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTacga                                                                                         <  1:3497038/57‑1 (MQ=255)

                                                    cTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTac                                                                                           <  1:3497156/55‑1 (MQ=255)

                                                     tGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGt                                            >  1:3497471/1‑101 (MQ=255)

                                                      gagaCCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTg                                                          <  2:3497122/86‑1 (MQ=255)

                                                      gagaCCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTg                                           <  1:3497320/101‑1 (MQ=255)

                                                         aCCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATa                                        <  1:3496905/101‑1 (MQ=255)

                                                          ccGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGt                                                                                <  2:3496899/60‑1 (MQ=255)

                                                          ccGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTc                                                                   <  2:3497181/73‑1 (MQ=255)

                                                          ccGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTg                                                          <  2:3497102/82‑1 (MQ=255)

                                                          ccGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCct                                                  <  2:3497080/90‑1 (MQ=255)

                                                          ccGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATaa                                       <  1:3497117/101‑1 (MQ=255)

                                                           cGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATaaa                                      <  1:3496994/101‑1 (MQ=255)

                                                            ggCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGtt                                                                     <  2:3496978/69‑1 (MQ=255)

                                                            ggCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAAc                                     <  1:3497115/101‑1 (MQ=255)

                                                            ggCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAAc                                     >  1:3497474/1‑101 (MQ=255)

                                                              cAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCa                                   <  2:3497160/101‑1 (MQ=255)

                                                              cAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCa                                   <  1:3497105/101‑1 (MQ=255)

                                                              cAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCa                                   <  1:3497087/101‑1 (MQ=255)

                                                               aGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGtt                                                                               <  2:3497030/56‑1 (MQ=255)

                                                               aGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAg                                  >  1:3497476/1‑101 (MQ=255)

                                                                gccgccGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGc                                 >  1:3497478/1‑101 (MQ=255)

                                                                gccgccGCAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGc                                 <  1:3497065/101‑1 (MQ=255)

                                                                  cgccgcAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGa                               >  2:3497480/1‑101 (MQ=255)

                                                                   gccgcAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGac                              >  2:3497482/1‑101 (MQ=255)

                                                                    ccgcAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGaca                             <  2:3497192/101‑1 (MQ=255)

                                                                       cAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCg                          <  2:3497232/101‑1 (MQ=255)

                                                                       cAGCGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCg                          >  1:3497485/1‑101 (MQ=255)

                                                                          cGGCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCa                       >  2:3497489/1‑101 (MQ=255)

                                                                           ggCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAg                      >  1:3497491/1‑101 (MQ=255)

                                                                           ggCCGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAg                      >  2:3497492/1‑101 (MQ=255)

                                                                             ccGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCa                    >  1:3497494/1‑101 (MQ=255)

                                                                              cGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCAc                   >  2:3497496/1‑101 (MQ=255)

                                                                              cGCAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCAc                   <  2:3497164/101‑1 (MQ=255)

                                                                                cAACGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCACGc                 >  1:3497498/1‑101 (MQ=255)

                                                                                 aaCGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCACGcc                <  1:3496795/101‑1 (MQ=255)

                                                                                 aaCGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCACGcc                <  2:3497060/101‑1 (MQ=255)

                                                                                 aaCGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCACGcc                <  1:3497023/101‑1 (MQ=255)

                                                                                   cGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCACGCCct              <  2:3497185/101‑1 (MQ=255)

                                                                                   cGGCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCACGCCct              <  2:3497331/101‑1 (MQ=255)

                                                                                     gCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCACGCCctct            >  2:3497504/1‑101 (MQ=255)

                                                                                     gCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCACGCCctct            <  1:3497021/101‑1 (MQ=255)

                                                                                     gCGTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCACGCCctct            >  1:3497503/1‑101 (MQ=255)

                                                                                       gTTAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCACGCCCTCTAc          >  1:3497507/1‑101 (MQ=255)

                                                                                         tAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCACGCCCTCTACGt        >  1:3497510/1‑101 (MQ=255)

                                                                                         tAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCACGCCCTCTACGt        <  1:3497281/101‑1 (MQ=255)

                                                                                         tAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCACGCCCTCTACGt        <  1:3497050/101‑1 (MQ=255)

                                                                                         tAGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCACGCCCTCTACGt        >  2:3497509/1‑101 (MQ=255)

                                                                                          aGCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCACGCCCTCTACGtt       >  2:3497512/1‑101 (MQ=255)

                                                                                           gCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCACGCCCTCTACGTTg      >  1:3497515/1‑101 (MQ=255)

                                                                                           gCTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCACGCCCTCTACGTTg      <  1:3497058/101‑1 (MQ=255)

                                                                                            cTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCACGCCCTCTACGTTGc     <  2:3497149/101‑1 (MQ=255)

                                                                                            cTTTCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCACGCCCTCTACGTTGc     >  1:3497517/1‑101 (MQ=255)

                                                                                             tttCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCACGCCCTCTACGTTGCg    >  1:3497521/1‑101 (MQ=255)

                                                                                               tCACAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCACGCCCTCTACGTTGCGTa  >  1:3497523/1‑101 (MQ=255)

CCATTTGATGGCTTCCTGCTGACCAACAACGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGCCGCAACGGCGTTAGCTTTCATAAAGCTACGACGACTGAGTTTCATGGTGTTTCCTCACCTTGCTCTTCCTGCTGGTGATAAACCAGCGACACCGCCAGCACGCCCTCTACGTTGCGTA  >  NC_000913/2302631‑2302826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`