Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,108,347 T→C D270G (GAC→GGC)  speC ← ornithine decarboxylase, biosynthetic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,108,3470TC97.1% 1210.6 / 15.5 312D270G (GAC→GGC) speCornithine decarboxylase, biosynthetic
Reads supporting (aligned to +/- strand):  ref base T (3/6);  new base C (133/170);  total (136/176)
Fisher's exact test for biased strand distribution p-value = 7.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.07e-01

Only 100 of 312 total aligned reads displayed.

ACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCG  >  NC_000913/3108249‑3108444
                                                                                                  |                                                                                                 
acaGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCg                                                                                                 >  1:4734636/1‑101 (MQ=255)
 caGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCgg                                                                                                <  2:4733709/101‑1 (MQ=255)
 caGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCgg                                                                                                <  2:4734338/101‑1 (MQ=255)
  aGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGc                                                                                               >  2:4734639/1‑101 (MQ=255)
    aTGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCtt                                                                                             >  1:4734642/1‑101 (MQ=255)
     tGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCttt                                                                                            >  1:4734645/1‑101 (MQ=255)
      gCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCtttt                                                                                           <  1:4733833/101‑1 (MQ=255)
       cccAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCttttt                                                                                          >  2:4734649/1‑101 (MQ=255)
        ccAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTc                                                                                         >  1:4734651/1‑101 (MQ=255)
        ccAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTc                                                                                         <  2:4733897/101‑1 (MQ=255)
         cAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCt                                                                                        <  2:4734124/101‑1 (MQ=255)
          aaCGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGTCGATACGGGCGCGGCAGGCCGGCTTTTTCTg                                                                                       >  1:4734657/1‑101 (MQ=255)
          aaCGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTg                                                                                       >  2:4734656/1‑101 (MQ=255)
           aCGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCttttt                                                                                          >  1:4734662/1‑97 (MQ=255)
            cGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGgc                                                                                     >  2:4734664/1‑101 (MQ=255)
             ggTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGgcg                                                                                    <  1:4734182/101‑1 (MQ=255)
              gTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGgcgc                                                                                   <  2:4734160/101‑1 (MQ=255)
              gTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGgcgc                                                                                   <  1:4734264/101‑1 (MQ=255)
              ctATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGgcgc                                                                                   >  1:4734669/2‑101 (MQ=255)
                 tCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAAc                                                                                >  2:4734673/1‑101 (MQ=255)
                 tCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAAc                                                                                <  1:4734099/101‑1 (MQ=255)
                   gATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGt                                                                              >  2:4734676/1‑101 (MQ=255)
                    aTCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTc                                                                             >  1:4734678/1‑101 (MQ=255)
                      cACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTcgc                                                                           >  1:4734684/1‑101 (MQ=255)
                      cACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTcgc                                                                           <  2:4734196/101‑1 (MQ=255)
                        ccTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGa                                                                         >  2:4734686/1‑101 (MQ=255)
                         cTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGaa                                                                        >  1:4734688/1‑101 (MQ=255)
                          tGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAAt                                                                       >  2:4734692/1‑101 (MQ=255)
                           gACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAAtt                                                                      <  1:4734134/101‑1 (MQ=255)
                             cGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTg                                                                    <  2:4734235/101‑1 (MQ=255)
                              gggCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGc                                                                   >  1:4734694/1‑101 (MQ=255)
                                gCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTg                                                                 <  1:4734312/101‑1 (MQ=255)
                                 cGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTgg                                                                <  2:4734313/101‑1 (MQ=255)
                                 cGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTgg                                                                >  2:4734698/1‑101 (MQ=255)
                                  gTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGgc                                                               <  2:4734032/101‑1 (MQ=255)
                                  gTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGgc                                                               <  2:4734493/101‑1 (MQ=255)
                                   ttATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGgcg                                                              <  2:4734206/101‑1 (MQ=255)
                                    tataGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGgcgc                                                             <  1:4734228/101‑1 (MQ=255)
                                    tataGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGgcgc                                                             >  1:4734703/1‑101 (MQ=255)
                                    tataGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGgcgc                                                             >  2:4734702/1‑101 (MQ=255)
                                    tataGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGgcgc                                                             <  2:4734178/101‑1 (MQ=255)
                                     ataGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCa                                                            <  2:4734294/101‑1 (MQ=255)
                                     ataGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCa                                                            <  2:4734174/101‑1 (MQ=255)
                                      taGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAg                                                           >  2:4734707/1‑101 (MQ=255)
                                        gACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGAt                                                         >  2:4734710/1‑101 (MQ=255)
                                            gTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTg                                                                 <  2:4733958/89‑1 (MQ=255)
                                            gTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATActc                                                     <  2:4734101/101‑1 (MQ=255)
                                             tGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATActct                                                    <  1:4734378/101‑1 (MQ=255)
                                              gCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCtt                                                   <  1:4734233/101‑1 (MQ=255)
                                              gCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCtt                                                   >  1:4734712/1‑101 (MQ=255)
                                               ccGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTc                                                  <  1:4734468/101‑1 (MQ=255)
                                               ccGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTc                                                  <  1:4734279/101‑1 (MQ=255)
                                                 gTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCAt                                                >  2:4734718/1‑101 (MQ=255)
                                                 gTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCAt                                                <  1:4734255/101‑1 (MQ=255)
                                                 gTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCAt                                                >  1:4734717/1‑101 (MQ=255)
                                                  tCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCAtt                                               <  2:4734298/101‑1 (MQ=255)
                                                   cATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTa                                              <  2:4734361/101‑1 (MQ=255)
                                                   cATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTa                                              <  2:4733995/101‑1 (MQ=255)
                                                    aTAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTaa                                             >  2:4734722/1‑101 (MQ=255)
                                                    aTAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTaa                                             <  1:4734207/101‑1 (MQ=255)
                                                     tAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTaaa                                            <  2:4734066/101‑1 (MQ=255)
                                                     tAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTaaa                                            <  1:4734074/101‑1 (MQ=255)
                                                        gTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACa                                         <  2:4734565/101‑1 (MQ=255)
                                                         ttCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAg                                        <  1:4734423/101‑1 (MQ=255)
                                                          tCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGt                                       >  2:4734727/1‑101 (MQ=255)
                                                           cccAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTg                                      <  1:4734245/101‑1 (MQ=255)
                                                           cccAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTg                                      <  1:4734447/101‑1 (MQ=255)
                                                            ccAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTgc                                     <  1:4734117/101‑1 (MQ=255)
                                                            ccAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTgc                                     <  1:4734051/101‑1 (MQ=255)
                                                            ccAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTgc                                     <  2:4734239/101‑1 (MQ=255)
                                                             cAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTgcg                                    <  2:4734118/101‑1 (MQ=255)
                                                              aGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTgcgc                                   >  2:4734732/1‑101 (MQ=255)
                                                               gCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCa                                  <  2:4734253/101‑1 (MQ=255)
                                                                cTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCAt                                 <  1:4734187/101‑1 (MQ=255)
                                                                cTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCAt                                 <  1:4734157/101‑1 (MQ=255)
                                                                 tGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATc                                <  2:4734350/101‑1 (MQ=255)
                                                                 tGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATc                                <  2:4734384/101‑1 (MQ=255)
                                                                 tGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATc                                >  1:4734734/1‑101 (MQ=255)
                                                                 tGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATc                                <  1:4734290/101‑1 (MQ=255)
                                                                  gAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACa                                         >  2:4734738/1‑91 (MQ=255)
                                                                    ataatCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAAt                             >  1:4734740/1‑101 (MQ=255)
                                                                    ataatCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAAt                             <  1:4734328/101‑1 (MQ=255)
                                                                     taatCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATa                            >  1:4734744/1‑101 (MQ=255)
                                                                          gCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGcc                       >  1:4734747/1‑101 (MQ=255)
                                                                          gCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGcc                       >  2:4734748/1‑101 (MQ=255)
                                                                            cAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCaa                     <  1:4733955/101‑1 (MQ=255)
                                                                               gCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGa                  >  2:4734752/1‑101 (MQ=255)
                                                                                cGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGaa                 <  1:4734299/101‑1 (MQ=255)
                                                                                 gATACGGGCGCGGCAGGCCGGCTTTTTCTGGCCCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGaaa                >  2:4734756/1‑101 (MQ=255)
                                                                                  aTACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAAc               >  1:4734758/1‑101 (MQ=255)
                                                                                  aTACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAAc               <  1:4734271/101‑1 (MQ=255)
                                                                                   tACGGGCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAAcc              >  2:4734760/1‑101 (MQ=255)
                                                                                      gggCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCaaa           >  1:4734767/1‑101 (MQ=255)
                                                                                      gggCGCGGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCaaa           >  1:4734763/1‑101 (MQ=255)
                                                                                          gcgGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACggg       <  2:4734184/101‑1 (MQ=255)
                                                                                          gcgGCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACggg       >  2:4734770/1‑101 (MQ=255)
                                                                                            ggCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGtt     <  1:4734358/101‑1 (MQ=255)
                                                                                            ggCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGtt     <  1:4734084/101‑1 (MQ=255)
                                                                                             gCAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTg    <  2:4734002/101‑1 (MQ=255)
                                                                                              cAGGCCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGc   <  2:4734448/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
ACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCG  >  NC_000913/3108249‑3108444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑