BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,478,945	(G)_5→4	coding (1099/1338 nt)	dsdX →	D‑serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,478,941	0	G	.	99.5%	1062.6 / ‑2.4	204	coding (1095/1338 nt)	dsdX	D‑serine transporter
Reads supporting (aligned to +/- strand): ref base G (0/1); new base . (106/97); total (106/98)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

Only 100 of 217 total aligned reads displayed.

TTATTCTCTCCAATATGCATATGCACCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGATGGGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCGGTTCAGGTGCAATTGGCTGC  >  NC_000913/2478844‑2479037

 tATTCTCTCCAATATGCATATGCACCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGATggggc                                                                                              >  1:3210167/1‑100 (MQ=255)

 tATTCTCTCCAATATGCATATGCACCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGATggggc                                                                                              <  1:3209754/101‑2 (MQ=255)

  aTTCTCTCCAATATGCATATGCACCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGATggggca                                                                                             >  2:3210168/1‑99 (MQ=255)

    tctctcCAATATGCATATGCACCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGATggggcaac                                                                                           >  1:3210171/1‑97 (MQ=255)

      tctcCAATATGCATATGCACCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGTAGTGGGCTCCGCTACCGTGGCAATGATgg                                                                                                 <  2:3209759/93‑1 (MQ=255)

      tctcCAATATGCATATGCACCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACgg                                                                                        >  2:3210174/1‑101 (MQ=255)

        tcCAATATGCATATGCACCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCa                                                                                      >  1:3210176/1‑101 (MQ=255)

         ccAATATGCATATGCACCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCaa                                                                                     <  1:3209768/101‑1 (MQ=255)

         ccAATATGCATATGCACCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCaa                                                                                     >  1:3210178/1‑101 (MQ=255)

           aaTATGCATATGCACCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAAtt                                                                                   >  2:3210181/1‑101 (MQ=255)

           aaTATGCATATGCACCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAAtt                                                                                   <  2:3209962/101‑1 (MQ=255)

            atatgcatatgcACCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAAttg                                                                                  <  2:3209830/101‑1 (MQ=255)

             tatgcatatgcaCCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAAttgt                                                                                 <  2:3210039/101‑1 (MQ=255)

              atgcatatgcaCCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAAttgtt                                                                                >  1:3210183/1‑101 (MQ=255)

              atgcatatgcaCCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAAttgtt                                                                                <  1:3209728/101‑1 (MQ=255)

               tgcatatgcaCCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAAttgttg                                                                               >  2:3210185/1‑101 (MQ=255)

                gcatatgcaCCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGc                                                                              >  1:3210186/1‑101 (MQ=255)

                   tatgcaCCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCAcc                                                                           >  1:3210189/1‑101 (MQ=255)

                     tgcaCCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCa                                                                         >  1:3210191/1‑101 (MQ=255)

                     tgcaCCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCa                                                                         <  1:3209782/101‑1 (MQ=255)

                      gcaCCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCAt                                                                        <  1:3209777/101‑1 (MQ=255)

                      gcaCCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCAt                                                                        <  2:3209806/101‑1 (MQ=255)

                        aCCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCAtgc                                                                      >  2:3210193/1‑101 (MQ=255)

                         cccGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCAtgct                                                                     <  1:3209417/101‑1 (MQ=255)

                          ccGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCAtgctg                                                                    <  1:3209951/101‑1 (MQ=255)

                          ccGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCAtgctg                                                                    >  1:3210195/1‑101 (MQ=255)

                             aTTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCg                                                                 <  2:3209875/101‑1 (MQ=255)

                              ttcttcTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGc                                                                >  1:3210198/1‑101 (MQ=255)

                               tcttctGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTCCCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCt                                                               <  2:3209748/101‑1 (MQ=255)

                               tcttctGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCt                                                               >  2:3210200/1‑101 (MQ=255)

                               tcttctGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCt                                                               <  2:3209802/101‑1 (MQ=255)

                                cttctGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTg                                                              >  1:3210201/1‑101 (MQ=255)

                                 ttctGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGt                                                             <  2:3209664/101‑1 (MQ=255)

                                   ctGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTAt                                                           >  2:3210205/1‑101 (MQ=255)

                                    tGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATc                                                          >  2:3210208/1‑101 (MQ=255)

                                    tGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATc                                                          >  1:3210206/1‑101 (MQ=255)

                                      gCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGTCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATccc                                                        <  2:3209667/101‑1 (MQ=255)

                                      gCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATccc                                                        <  1:3209809/101‑1 (MQ=255)

                                           gTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACAt                                                   >  1:3210211/1‑101 (MQ=255)

                                           gTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACAt                                                   <  2:3209929/101‑1 (MQ=255)

                                            ttAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATc                                                  >  1:3210213/1‑101 (MQ=255)

                                             tAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCa                                                 <  1:3209688/101‑1 (MQ=255)

                                             tAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCa                                                 <  2:3209714/101‑1 (MQ=255)

                                             tAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCa                                                 >  1:3210216/1‑101 (MQ=255)

                                             tAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCa                                                 <  1:3209887/101‑1 (MQ=255)

                                              aGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAg                                                >  2:3210217/1‑101 (MQ=255)

                                                tGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCt                                                               >  1:3210219/1‑84 (MQ=255)

                                                tGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGcc                                              <  2:3209907/101‑1 (MQ=255)

                                                   ctctTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCgg                                           <  1:3210060/101‑1 (MQ=255)

                                                   ctctTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCgg                                           >  2:3210223/1‑101 (MQ=255)

                                                    tctTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGa                                          >  1:3210224/1‑101 (MQ=255)

                                                     ctTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGaa                                         <  1:3209823/101‑1 (MQ=255)

                                                      ttATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGaaa                                        >  1:3210229/1‑101 (MQ=255)

                                                      ttATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGaaa                                        <  1:3209829/101‑1 (MQ=255)

                                                      ttATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGaaa                                        >  2:3210227/1‑101 (MQ=255)

                                                      ttATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGaaa                                        <  2:3209701/101‑1 (MQ=255)

                                                      ttATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGaaa                                        >  2:3210230/1‑101 (MQ=255)

                                                        aTTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCCCCCATGCTGCCGCTGTATCCCGACATCAGCCCg                                            <  1:3209988/95‑1 (MQ=255)

                                                        aTTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAatt                                      >  2:3210233/1‑101 (MQ=255)

                                                         ttCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAatta                                     <  1:3209650/101‑1 (MQ=255)

                                                         ttCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAatta                                     >  2:3210236/1‑101 (MQ=255)

                                                         ttCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAatta                                     <  1:3210131/101‑1 (MQ=255)

                                                          tCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAattat                                    >  1:3210237/1‑101 (MQ=255)

                                                           cTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAattatt                                   <  1:3209678/101‑1 (MQ=255)

                                                            tGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTg                                  >  1:3210239/1‑101 (MQ=255)

                                                              cATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGc                                 >  1:3210243/1‑100 (MQ=255)

                                                              cATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCg                                <  1:3209838/101‑1 (MQ=255)

                                                              cATGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCg                                <  1:3209603/101‑1 (MQ=255)

                                                               aTGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGa                               >  2:3210244/1‑101 (MQ=255)

                                                                tGCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATCATTGCGAt                              >  1:3210246/1‑101 (MQ=255)

                                                                 gCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGAtt                             >  2:3210249/1‑101 (MQ=255)

                                                                 gCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGAtt                             >  1:3210250/1‑101 (MQ=255)

                                                                 gCGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGAtt                             <  1:3210051/101‑1 (MQ=255)

                                                                  cGGCAGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTg                            <  1:3209926/101‑1 (MQ=255)

                                                                      aGTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTAt                        <  1:3209872/101‑1 (MQ=255)

                                                                       gTGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCaaat                                                                                   >  2:3210252/1‑39 (MQ=38)

                                                                        tGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCg                      <  2:3209767/101‑1 (MQ=255)

                                                                        tGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCg                      <  2:3210191/101‑1 (MQ=255)

                                                                        tGGGCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCg                      >  1:3210255/1‑101 (MQ=255)

                                                                          ggCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCGGt                    <  1:3209738/101‑1 (MQ=255)

                                                                           gCTCCGCTACCGTGGCACTGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCGGtt                   <  2:3209773/101‑1 (MQ=255)

                                                                           gCTCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCGGtt                   >  1:3210257/1‑101 (MQ=255)

                                                                             tCCGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCGGTTCa                 <  1:3209702/101‑1 (MQ=255)

                                                                               cGCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCGGTTCAgg               <  2:3209816/101‑1 (MQ=255)

                                                                                gCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCGGTTCAGGt              >  1:3210261/1‑101 (MQ=255)

                                                                                gCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCGGTTCAGGt              <  2:3210091/101‑1 (MQ=255)

                                                                                gCTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCGGTTCAGGt              >  2:3210260/1‑101 (MQ=255)

                                                                                 cTACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTa                         <  2:3209843/89‑1 (MQ=255)

                                                                                  tACCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCGGTTCAGGTGc            >  2:3210265/1‑101 (MQ=255)

                                                                                   aCCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCGGTTCCGGTGCa           <  1:3209801/101‑1 (MQ=255)

                                                                                   aCCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCGGTTCAGGTGCa           <  1:3210042/101‑1 (MQ=255)

                                                                                   aCCGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCGGTTCAGGTGCa           >  2:3210267/1‑101 (MQ=255)

                                                                                     cGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCCCCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCGGTTCAGGTGCAAt         >  2:3210269/1‑101 (MQ=255)

                                                                                     cGTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCGGTTCAGGTGCAAt         <  1:3209867/101‑1 (MQ=255)

                                                                                      gTGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCGGTTCAGGTGCAAtt        >  1:3210270/1‑101 (MQ=255)

                                                                                       tGGCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCGGTTCAGGTGCAATTg       >  2:3210273/1‑101 (MQ=255)

                                                                                         gCAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCGGTTCAGGTGCAATTGGc     >  1:3210274/1‑101 (MQ=255)

                                                                                          cAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCGGTTCAGGTGCAATTGGCt    <  1:3209792/101‑1 (MQ=255)

                                                                                          cAATGAT‑GGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCGGTTCAGGTGCAATTGGCt    >  2:3210277/1‑101 (MQ=255)

                                                                                            atgat‑gGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCGGTTCAGGTGCAATTGGCTGc  >  1:3210280/1‑101 (MQ=255)

TTATTCTCTCCAATATGCATATGCACCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGATGGGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATTATTGCGATTGCTATCGGTTCAGGTGCAATTGGCTGC  >  NC_000913/2478844‑2479037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`