BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,486,481	(A)_7→8	coding (2108/3594 nt)	evgS →	sensory histidine kinase EvgS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,486,474	1	.	A	99.2%	1031.1 / 0.3	246	coding (2101/3594 nt)	evgS	sensory histidine kinase EvgS
Reads supporting (aligned to +/- strand): ref base . (2/0); new base A (122/122); total (124/122)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

Only 100 of 274 total aligned reads displayed.

GCATACATTATGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAG‑AAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATGGG  >  NC_000913/2486374‑2486568

gCATACATTATGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAg                                                                                                 >  2:3220193/1‑101 (MQ=255)

gCATACATTATGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAg                                                                                                 <  2:3219901/101‑1 (MQ=255)

 cATACATTATGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAG‑a                                                                                               <  2:3219610/101‑1 (MQ=255)

 cATACATTATGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAG‑a                                                                                               <  1:3219687/101‑1 (MQ=255)

  aTACATTATGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAG‑aa                                                                                              >  2:3220196/1‑101 (MQ=255)

  aTACATTATGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAG‑aa                                                                                              <  1:3219739/101‑1 (MQ=255)

      aTTATGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAG‑aaaaaa                                                                                          >  2:3220199/1‑101 (MQ=255)

          tGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAG‑aaaaaaaata                                                                                      <  2:3219870/101‑4 (MQ=255)

          tGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAG‑aaaaaaaata                                                                                      <  2:3219888/101‑4 (MQ=255)

          tGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAG‑aaaaaaaata                                                                                      >  1:3220203/1‑98 (MQ=255)

             aaTCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAg                                                                                    <  2:3219956/101‑1 (MQ=255)

             aaTCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAg                                                                                    >  1:3220205/1‑101 (MQ=255)

             aaTCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAg                                                                                    >  2:3220207/1‑101 (MQ=255)

                 ttCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGAt                                                                                <  2:3219712/101‑1 (MQ=255)

                   ccTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATaa                                                                              >  2:3220212/1‑101 (MQ=255)

                    cTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATaaa                                                                             >  1:3220213/1‑101 (MQ=255)

                     tGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAg                                                                            >  2:3220216/1‑101 (MQ=255)

                       cAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGc                                                                          >  2:3220219/1‑101 (MQ=255)

                          gTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTAc                                                                       >  1:3220223/1‑101 (MQ=255)

                           tGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTAcc                                                                      >  2:3220224/1‑101 (MQ=255)

                            gACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCg                                                                     <  2:3219850/101‑1 (MQ=255)

                            gACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCg                                                                     >  1:3220226/1‑101 (MQ=255)

                             aCAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGt                                                                    >  1:3220231/1‑101 (MQ=255)

                              cAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGtg                                                                   <  1:3219648/101‑2 (MQ=255)

                              cAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTa                                                                   <  1:3219977/101‑1 (MQ=255)

                               aaTGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAg                                                                  >  1:3220234/1‑101 (MQ=255)

                                aTGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGc                                                                 <  2:3219882/101‑1 (MQ=255)

                                 tGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCa                                                                <  2:3219827/101‑1 (MQ=255)

                                  gCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCaa                                                               <  2:3219764/101‑1 (MQ=255)

                                  gCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCaa                                                               >  2:3220235/1‑101 (MQ=255)

                                  gCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCaa                                                               >  2:3220237/1‑101 (MQ=255)

                                    aGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCaaaa                                                             >  1:3220242/1‑101 (MQ=255)

                                      tatataTTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAg                                                           >  2:3220245/1‑101 (MQ=255)

                                      tatataTTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAg                                                           >  1:3220244/1‑101 (MQ=255)

                                       atatatTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGt                                                          <  1:3219723/101‑1 (MQ=255)

                                       atatatTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGt                                                          >  2:3220249/1‑101 (MQ=255)

                                        tatatTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTc                                                         <  2:3219937/101‑1 (MQ=255)

                                         atatTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCa                                                        <  1:3219889/101‑1 (MQ=255)

                                         atatTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCa                                                        <  2:3219867/101‑1 (MQ=255)

                                         atatTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCa                                                        >  1:3220252/1‑101 (MQ=255)

                                            tttGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGtt                                                     >  1:3220256/1‑101 (MQ=255)

                                            tttGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGtt                                                     >  2:3220257/1‑101 (MQ=255)

                                             ttGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGttt                                                    <  2:3219924/101‑1 (MQ=255)

                                             ttGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGttt                                                    <  1:3219802/101‑1 (MQ=255)

                                               gtgGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCt                                                  >  1:3220261/1‑101 (MQ=255)

                                                tgGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTg                                                 >  2:3220262/1‑101 (MQ=255)

                                                 ggTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTgg                                                >  1:3220265/1‑101 (MQ=255)

                                                 ggTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTgg                                                <  2:3219688/101‑1 (MQ=255)

                                                   ttGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCa                                              <  2:3219969/101‑1 (MQ=255)

                                                   ttGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCa                                              <  1:3219783/101‑1 (MQ=255)

                                                   ttGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCa                                              >  2:3220269/1‑101 (MQ=255)

                                                    tGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCaa                                             <  2:3219514/101‑1 (MQ=255)

                                                     ggCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAAc                                            <  1:3219533/101‑1 (MQ=255)

                                                     ggCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAAc                                            <  1:3220033/101‑1 (MQ=255)

                                                      gCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACg                                           <  2:3219834/101‑1 (MQ=255)

                                                       cAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGa                                          >  1:3220271/1‑101 (MQ=255)

                                                        aaGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGAt                                         >  2:3220274/1‑101 (MQ=255)

                                                        aaGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGAt                                         >  1:3220277/1‑101 (MQ=255)

                                                          gATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGa                                       >  1:3220280/1‑101 (MQ=255)

                                                            tatTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGt                                     <  2:3219786/101‑1 (MQ=255)

                                                            tatTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGt                                     >  2:3220282/1‑101 (MQ=255)

                                                            tatTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGt                                     <  1:3219457/101‑1 (MQ=255)

                                                            tatTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGt                                     <  1:3219991/101‑1 (MQ=255)

                                                             atTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTc                                    >  2:3220284/1‑101 (MQ=255)

                                                             atTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTc                                    <  2:3219857/101‑1 (MQ=255)

                                                              ttACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCa                                   >  1:3220286/1‑101 (MQ=255)

                                                               tACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCAc                                  >  1:3220292/1‑101 (MQ=255)

                                                               tACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCAc                                  >  1:3220289/1‑101 (MQ=255)

                                                               tACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCAc                                  <  1:3219838/101‑1 (MQ=255)

                                                               tACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCAc                                  <  2:3219839/101‑1 (MQ=255)

                                                                 cTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGa                                <  1:3219929/101‑1 (MQ=255)

                                                                  tGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGaa                               <  1:3219579/101‑1 (MQ=255)

                                                                   gAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGaaa                              <  2:3220093/101‑1 (MQ=255)

                                                                    aaaCGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAAt                             <  2:3219946/101‑1 (MQ=255)

                                                                     aaCGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATa                            >  2:3220298/1‑101 (MQ=255)

                                                                     aaCGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATa                            >  1:3220297/1‑101 (MQ=255)

                                                                      aCGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATaa                           <  2:3220129/101‑1 (MQ=255)

                                                                        gcgTGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGa                         >  1:3220301/1‑101 (MQ=255)

                                                                           tGATCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAaca                      <  2:3219904/101‑1 (MQ=255)

                                                                             aTCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACAcc                    >  1:3220305/1‑101 (MQ=255)

                                                                              tCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCa                   >  2:3220306/1‑101 (MQ=255)

                                                                              tCTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCa                   <  1:3219849/101‑1 (MQ=255)

                                                                               cTAATTAATGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCaa                  <  2:3219749/101‑1 (MQ=255)

                                                                                 aattaatGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATa                <  1:3219861/101‑1 (MQ=255)

                                                                                  attaatGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATaa               >  2:3220311/1‑101 (MQ=255)

                                                                                   ttaatGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAg              <  2:3219822/101‑1 (MQ=255)

                                                                                   ttaatGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAg              >  1:3220313/1‑101 (MQ=255)

                                                                                    taatGCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGc             >  2:3220314/1‑101 (MQ=255)

                                                                                        gCACTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATa                <  1:3219913/94‑1 (MQ=255)

                                                                                          aCTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCtatt       >  1:3220319/1‑101 (MQ=255)

                                                                                          aCTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCtatt       >  2:3220320/1‑101 (MQ=255)

                                                                                          aCTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCtatt       <  2:3220013/101‑1 (MQ=255)

                                                                                           cTCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCtatta      <  1:3220092/101‑1 (MQ=255)

                                                                                            tCGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCtattat     <  2:3220039/101‑1 (MQ=255)

                                                                                             cGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATg    >  2:3220324/1‑101 (MQ=255)

                                                                                             cGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATg    >  1:3220326/1‑101 (MQ=255)

                                                                                             cGAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATg    <  1:3219960/101‑1 (MQ=255)

                                                                                              gAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATgg   <  1:3219892/101‑1 (MQ=255)

                                                                                              gAGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATgg   <  1:3219947/101‑1 (MQ=255)

                                                                                               aGGTAGAAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATggg  <  1:3219810/101‑1 (MQ=255)

GCATACATTATGCAATCTTCCTGCAAGTGACAATGCAGTATATATTTGTGGTTGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAG‑AAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTATTATGGG  >  NC_000913/2486374‑2486568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`