BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,484,088	(C)_6→7	coding (887/1023 nt)	yheT →	putative hydrolase YheT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,484,082	1	.	C	98.7%	951.0 / 2.8	235	coding (881/1023 nt)	yheT	putative hydrolase YheT
Reads supporting (aligned to +/- strand): ref base . (0/3); new base C (112/120); total (112/123)
Fisher's exact test for biased strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

GCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCA  >  NC_000913/3483982‑3484176

 ccATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGtc‑t‑‑c                                                                                                <  1:4548112/101‑1 (MQ=255)

  cATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑‑c‑c                                                                                              >  2:4548545/1‑101 (MQ=255)

   aTGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑‑c‑cc                                                                                             >  1:4548546/1‑101 (MQ=255)

      ccGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑‑c‑ccccc                                                                                          >  2:4548550/1‑101 (MQ=255)

      ccGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑‑c‑ccccc                                                                                          <  2:4548196/101‑1 (MQ=255)

      ccGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑‑c‑ccccc                                                                                          <  2:4548208/101‑1 (MQ=255)

       cGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑‑c‑cccccc                                                                                         <  1:4547871/101‑2 (MQ=255)

        gATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑‑c‑ccccccg                                                                                        >  1:4548554/1‑99 (MQ=255)

        gATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑‑c‑ccccccg                                                                                        <  1:4548222/101‑3 (MQ=255)

         aTGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑‑c‑ccccccgc                                                                                       >  2:4548555/1‑98 (MQ=255)

             tGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGt                                                                                    >  1:4548559/1‑101 (MQ=255)

             tGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGt                                                                                    <  2:4547948/101‑1 (MQ=255)

                 ccGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAg                                                                                <  2:4548170/101‑1 (MQ=255)

                 ccGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAg                                                                                <  1:4548165/101‑1 (MQ=255)

                     aTCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑‑c‑ccccc                                                                                          >  2:4548562/1‑86 (MQ=255)

                      tCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCa                                                                           <  2:4548307/101‑1 (MQ=255)

                      tCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCa                                                                           >  1:4548563/1‑101 (MQ=255)

                      tCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCa                                                                           >  2:4548565/1‑101 (MQ=255)

                      tCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCa                                                                           <  1:4548311/101‑1 (MQ=255)

                        gCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAac                                                                         >  2:4548568/1‑101 (MQ=255)

                         ccAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAact                                                                        <  1:4548182/101‑1 (MQ=255)

                          cAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCC‑CCCCCGCAGGTGGAGTATCAact                                                                        <  1:4548139/101‑1 (MQ=255)

                           aaaCCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAactga                                                                      >  1:4548573/1‑101 (MQ=255)

                           aaaCCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAactga                                                                      >  1:4548571/1‑101 (MQ=255)

                            aaCCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAactgac                                                                     <  1:4548262/101‑1 (MQ=255)

                                gACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGaa                                                                 <  1:4548281/101‑1 (MQ=255)

                                gACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGaa                                                                 >  1:4548577/1‑101 (MQ=255)

                                   gCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACAt                                                              >  2:4548584/1‑101 (MQ=255)

                                   gCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACAt                                                              <  1:4548156/101‑1 (MQ=255)

                                   gCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACAt                                                              >  2:4548582/1‑101 (MQ=255)

                                    cTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATg                                                             <  1:4548447/101‑1 (MQ=255)

                                     tGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAact                                                                        <  2:4548137/89‑1 (MQ=255)

                                     tGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATgg                                                            >  1:4548588/1‑101 (MQ=255)

                                     tGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATgg                                                            >  2:4548587/1‑101 (MQ=255)

                                      gATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGc                                                           <  1:4548147/101‑1 (MQ=255)

                                      gATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGc                                                           <  1:4548357/101‑1 (MQ=255)

                                         tattCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGt                                                        >  1:4548591/1‑101 (MQ=255)

                                         tattCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGt                                                        <  2:4548213/101‑1 (MQ=255)

                                         tattCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGt                                                        <  1:4548075/101‑1 (MQ=255)

                                            tCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCAt                                                     <  1:4548054/101‑1 (MQ=255)

                                              aCGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGt                                                   >  1:4548595/1‑101 (MQ=255)

                                                gCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTg                                                 <  2:4548111/101‑1 (MQ=255)

                                                 ccAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTgg                                                >  2:4548599/1‑101 (MQ=255)

                                                  cAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGc                                               >  1:4548603/1‑101 (MQ=255)

                                                  cAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGc                                               <  1:4548150/101‑1 (MQ=255)

                                                   aaaGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAg                                                                                <  2:4548060/67‑1 (MQ=255)

                                                   aaaGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCt                                              >  1:4548605/1‑101 (MQ=255)

                                                   aaaGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCt                                              >  2:4548606/1‑101 (MQ=255)

                                                    aaGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCtt                                             <  1:4548083/101‑1 (MQ=255)

                                                    aaGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCtt                                             <  1:4548325/101‑1 (MQ=255)

                                                    aaGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCtt                                             >  2:4548609/1‑101 (MQ=255)

                                                       aCGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTAt                                          <  1:4548189/101‑1 (MQ=255)

                                                         gATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTg                                        <  2:4548577/101‑1 (MQ=255)

                                                         gATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTg                                        <  1:4548288/101‑1 (MQ=255)

                                                          aTCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTgg                                       >  1:4548615/1‑101 (MQ=255)

                                                          aTCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTgg                                       <  2:4548131/101‑1 (MQ=255)

                                                           tCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGc                                      >  2:4548616/1‑101 (MQ=255)

                                                            ccGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCaa                                                                          >  2:4548620/1‑64 (MQ=255)

                                                            ccGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCg                                     <  1:4548246/101‑1 (MQ=255)

                                                             cGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGGGGAg                                                                                >  1:4548621/1‑57 (MQ=255)

                                                              gTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGt                                   <  2:4548221/101‑1 (MQ=255)

                                                              gTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGt                                   <  2:4548227/101‑1 (MQ=255)

                                                              gTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGt                                   <  2:4548243/101‑1 (MQ=255)

                                                               tttATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAactgact                                                                    <  1:4548484/67‑1 (MQ=255)

                                                               tttATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTa                                  >  1:4548624/1‑101 (MQ=255)

                                                                 tATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACa                                <  1:4547976/101‑1 (MQ=255)

                                                                 tATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACa                                >  1:4548628/1‑101 (MQ=255)

                                                                  aTGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACAt                               >  2:4548630/1‑101 (MQ=255)

                                                                    ggATCATCAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTAc                                 >  1:4548633/1‑97 (MQ=255)

                                                                      atcatcAGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTAATTGGCGGTACATTACt                           <  2:4548337/101‑1 (MQ=255)

                                                                       tcatcaGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACtt                          <  1:4548251/101‑1 (MQ=255)

                                                                        catcaGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTc                         >  2:4548635/1‑101 (MQ=255)

                                                                        catcaGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTc                         <  1:4548371/101‑1 (MQ=255)

                                                                         atcaGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCa                        >  1:4548637/1‑101 (MQ=255)

                                                                          tcaGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCAt                       >  2:4548639/1‑101 (MQ=255)

                                                                           caGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATc                      <  1:4548301/101‑1 (MQ=255)

                                                                           caGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATc                      <  1:4548403/101‑1 (MQ=255)

                                                                            aGGTGATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATcc                     <  2:4548273/101‑1 (MQ=255)

                                                                             ggTGATCCCGAAACCGGAAAGTCGC‑‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCg                    <  1:4548204/101‑1 (MQ=255)

                                                                                gATCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCaa                 >  1:4548643/1‑101 (MQ=255)

                                                                                 aTCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCaaa                >  1:4548647/1‑101 (MQ=255)

                                                                                  tCCCGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAAt               >  2:4548648/1‑101 (MQ=255)

                                                                                    ccGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAAtgt             <  1:4548350/101‑1 (MQ=255)

                                                                                    ccGAAACCGGAAAGTC‑T‑‑CACCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAAtgt             <  1:4548133/101‑1 (MQ=255)

                                                                                     cGAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAAtgtg            >  1:4548651/1‑101 (MQ=255)

                                                                                      gAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAAtg              <  1:4548177/98‑1 (MQ=255)

                                                                                      gAAACCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTgg           >  1:4548655/1‑101 (MQ=255)

                                                                                       aaaCCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGc          >  2:4548658/1‑101 (MQ=255)

                                                                                       aaaCCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGCATCAACTGACTGAACATGGCGGTCATGTTg                                                 >  1:4548659/1‑62 (MQ=255)

                                                                                        aaCCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCt         <  2:4548347/101‑1 (MQ=255)

                                                                                         aCCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTg        <  2:4548446/101‑1 (MQ=255)

                                                                                         aCCGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTg        <  1:4548201/101‑1 (MQ=255)

                                                                                          ccGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTgg       >  2:4548662/1‑101 (MQ=255)

                                                                                          ccGGAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTgg       >  1:4548664/1‑101 (MQ=255)

                                                                                            ggAAAGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGGACATTACTTCAt                       >  2:4548668/1‑83 (MQ=255)

                                                                                              aaaGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTgg       >  2:4548672/1‑97 (MQ=255)

                                                                                              aaaGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTc   >  1:4548671/1‑101 (MQ=255)

                                                                                              aaaGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTc   <  2:4548367/101‑1 (MQ=255)

                                                                                               aaGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCa  <  2:4548287/101‑1 (MQ=255)

                                                                                               aaGTC‑TC‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCa  <  2:4548418/101‑1 (MQ=255)

GCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑‑C‑CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCA  >  NC_000913/3483982‑3484176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`