Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,606,215 (G)8→9 noncoding (72/87 nt) leuP ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,606,2071.G97.8% 703.2 / 6.5 182noncoding (80/87 nt)leuPtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (1/3);  new base G (117/61);  total (118/64)
Fisher's exact test for biased strand distribution p-value = 1.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 214 total aligned reads displayed.

ACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAT  >  NC_000913/4606107‑4606302
                                                                                                     |                                                                                                
aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGa                                                                                                   <  1:6105861/101‑1 (MQ=255)
            acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGa                                                                                                   >  2:6106258/1‑89 (MQ=255)
            acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gggggggggact                                                                                     >  1:6106257/1‑97 (MQ=255)
            acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gggggggggact                                                                                     <  1:6105940/101‑5 (MQ=255)
              acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGa                                                                                                   >  1:6106262/1‑87 (MQ=255)
               cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGa                                                                                   <  2:6105788/101‑1 (MQ=255)
                tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGaa                                                                                  >  1:6106264/1‑101 (MQ=255)
                  acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGa                                                                                                   >  1:6106267/1‑83 (MQ=255)
                   cacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAAccc                                                                               <  2:6105863/101‑1 (MQ=255)
                     ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAAcccc                                                                              <  2:6105813/100‑1 (MQ=255)
                      cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCa                                                                            >  1:6106271/1‑101 (MQ=255)
                       tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCAc                                                                           <  1:6105954/101‑1 (MQ=255)
                       tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCAc                                                                           <  2:6105871/101‑1 (MQ=255)
                       tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCAc                                                                           <  2:6105818/101‑1 (MQ=255)
                            cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCg                                                                      <  2:6105883/101‑1 (MQ=255)
                            cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCg                                                                      >  1:6106279/1‑101 (MQ=255)
                             tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGGACTTGAACCCCCACGTCCg                                                                      >  1:6106282/1‑101 (MQ=255)
                             tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGCCTTGAAc                                                                                 >  2:6106283/1‑89 (MQ=38)
                              aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGa                                                                                   >  2:6106286/1‑86 (MQ=255)
                               gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACg                                                                          >  1:6106288/1‑94 (MQ=255)
                                 gcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGa                                                                 <  2:6106232/101‑1 (MQ=255)
                                 gcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGa                                                                 >  1:6106290/1‑101 (MQ=255)
                                  cgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGaa                                                                <  2:6105875/101‑1 (MQ=255)
                                   gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑ggg                                                                                              <  1:6105899/69‑1 (MQ=255)
                                    tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑ggggggggg                                                                                        >  2:6106292/1‑73 (MQ=255)
                                     cTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAacac                                                             >  2:6106294/1‑101 (MQ=255)
                                      tACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                            >  1:6106296/1‑101 (MQ=255)
                                       aCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTa                                                           >  1:6106298/1‑101 (MQ=255)
                                        ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                          >  1:6106302/1‑101 (MQ=255)
                                        ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                          >  2:6106301/1‑101 (MQ=255)
                                        ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                          <  2:6105700/101‑1 (MQ=255)
                                          aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAaca                                                        >  1:6106306/1‑101 (MQ=255)
                                          aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAaca                                                        >  1:6106308/1‑101 (MQ=255)
                                          aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAaca                                                        >  2:6106309/1‑101 (MQ=255)
                                           aTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAacac                                                       >  1:6106314/1‑101 (MQ=255)
                                             tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                     >  2:6106319/1‑101 (MQ=255)
                                             tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                     >  2:6106317/1‑101 (MQ=255)
                                              ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTg                                                    >  2:6106321/1‑101 (MQ=255)
                                               cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGt                                                                     <  1:6106062/83‑1 (MQ=255)
                                               cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGa                                                   >  1:6106322/1‑101 (MQ=255)
                                                 ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAg                                                 <  1:6105887/101‑1 (MQ=255)
                                                  cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGaa                                                                                  >  2:6106325/1‑67 (MQ=255)
                                                  cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGc                                                <  1:6105855/101‑1 (MQ=255)
                                                   aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGc                                                >  1:6106326/1‑101 (MQ=255)
                                                    ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTGGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCAc                                                                           <  2:6105772/72‑1 (MQ=37)
                                                    ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                              >  1:6106328/1‑101 (MQ=255)
                                                     cTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg                                             >  2:6106330/1‑101 (MQ=255)
                                                     cTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gggggggggg                                                                                       >  2:6106332/1‑56 (MQ=255)
                                                      ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg                                             >  1:6106334/1‑101 (MQ=255)
                                                      ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                            <  2:6106073/101‑1 (MQ=255)
                                                       tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg                                           <  1:6106020/101‑1 (MQ=255)
                                                       tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg                                           <  1:6105948/101‑1 (MQ=255)
                                                        cGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg                                           >  1:6106338/1‑101 (MQ=255)
                                                         gCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcgc                                          >  2:6106339/1‑101 (MQ=255)
                                                          cacaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGt                                        >  1:6106343/1‑101 (MQ=255)
                                                           acaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTc                                       >  2:6106345/1‑101 (MQ=255)
                                                             aGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑ggggggggg                                                                                        >  1:6106348/1‑48 (MQ=255)
                                                               tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                   >  2:6106351/1‑101 (MQ=255)
                                                                cATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                  >  2:6106354/1‑101 (MQ=255)
                                                                cATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                  >  1:6106352/1‑101 (MQ=255)
                                                                   cTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑ggggggggg                                                                                        >  2:6106357/1‑42 (MQ=255)
                                                                    ttACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                              >  2:6106360/1‑101 (MQ=255)
                                                                    ttACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                              >  1:6106358/1‑101 (MQ=255)
                                                                    ttACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                              <  2:6106005/101‑1 (MQ=255)
                                                                     tACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                         >  1:6106364/1‑89 (MQ=255)
                                                                     tACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                             >  2:6106363/1‑101 (MQ=255)
                                                                      aCTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                            >  2:6106366/1‑101 (MQ=255)
                                                                       cTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAtt                               <  2:6105933/97‑1 (MQ=255)
                                                                         ttttttGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                         >  1:6106369/1‑101 (MQ=255)
                                                                          tttttGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                        >  2:6106373/1‑101 (MQ=255)
                                                                           ttttGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAATACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                       >  1:6106376/1‑101 (MQ=255)
                                                                           ttttGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                       <  1:6105816/101‑1 (MQ=255)
                                                                            tttGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                      >  2:6106378/1‑101 (MQ=255)
                                                                              tGATATCGCCTCGTTTGGTGCGAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcgc                                          >  2:6106380/1‑80 (MQ=17)
                                                                               gATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                   <  1:6105916/101‑1 (MQ=255)
                                                                                atatCGCCTGGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc                  <  1:6105828/101‑1 (MQ=255)
                                                                                atatCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc                  <  2:6106290/101‑1 (MQ=255)
                                                                                 tatCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                 >  2:6106383/1‑101 (MQ=255)
                                                                                 tatCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                 >  1:6106384/1‑101 (MQ=255)
                                                                                  atCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc                >  2:6106388/1‑101 (MQ=255)
                                                                                  atCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc                >  1:6106387/1‑101 (MQ=255)
                                                                                   tCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCa               <  2:6105629/101‑1 (MQ=255)
                                                                                    cGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg              >  1:6106390/1‑101 (MQ=255)
                                                                                     gCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt             >  1:6106394/1‑101 (MQ=255)
                                                                                     gCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt             >  2:6106393/1‑101 (MQ=255)
                                                                                     gCCTCGTTTGGTGCGA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                    <  1:6105994/93‑1 (MQ=40)
                                                                                      ccTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg            <  1:6105961/101‑1 (MQ=255)
                                                                                       cTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc           >  1:6106396/1‑101 (MQ=255)
                                                                                        tCGTTTGGTGCGAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcgc                                          <  2:6106078/70‑1 (MQ=14)
                                                                                        tCGTTTGGTGCGAG‑GGGGGGGGCCTTGAACCCCCACGTCCGTAAGAACACTaa                                                          >  2:6106398/1‑53 (MQ=14)
                                                                                        tCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                     <  2:6106082/58‑1 (MQ=14)
                                                                                          gTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg        >  1:6106400/1‑101 (MQ=255)
                                                                                           tttGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                  <  2:6105901/74‑1 (MQ=255)
                                                                                           tttGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGa         <  1:6105782/99‑1 (MQ=255)
                                                                                           tttGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGc       <  1:6105765/101‑1 (MQ=255)
                                                                                            ttGGTGCGAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                   <  2:6105909/89‑1 (MQ=11)
                                                                                            ttGGTGCGAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGc       <  2:6105894/101‑1 (MQ=255)
                                                                                            ttGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt             <  1:6105980/94‑1 (MQ=255)
                                                                                             tGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                    <  2:6105995/86‑1 (MQ=255)
                                                                                             tGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCaa     >  1:6106403/1‑101 (MQ=255)
                                                                                                     |                                                                                                
ACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAT  >  NC_000913/4606107‑4606302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑