BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,457,607	T→C	H518R (CAC→CGC)	fadJ ←	3‑hydroxyacyl‑CoA dehydrogenase FadJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,457,607	0	T	C	100.0%	899.8 / NA	218	H518R (CAC→CGC)	fadJ	3‑hydroxyacyl‑CoA dehydrogenase FadJ
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (110/108); total (110/108)

Only 100 of 218 total aligned reads displayed.

TACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGAC  >  NC_000913/2457510‑2457704

tACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGct                                                                                                >  2:3182331/1‑101 (MQ=255)

tACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGct                                                                                                <  1:3182010/101‑1 (MQ=255)

  cAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGctct                                                                                              >  2:3182333/1‑101 (MQ=255)

  cAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGctct                                                                                              <  2:3182020/101‑1 (MQ=255)

  cAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGctct                                                                                              <  1:3182024/101‑1 (MQ=255)

   aGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTa                                                                                             >  1:3182336/1‑101 (MQ=255)

    ggAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTAc                                                                                            <  1:3181686/101‑1 (MQ=255)

    ggAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTAc                                                                                            >  2:3182337/1‑101 (MQ=255)

      aataatTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTAccc                                                                                          <  1:3181933/101‑1 (MQ=255)

      aataatTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTAccc                                                                                          <  1:3181912/101‑1 (MQ=255)

      aataatTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTAccc                                                                                          >  2:3182341/1‑101 (MQ=255)

       ataatTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCg                                                                                         <  1:3181943/101‑1 (MQ=255)

        taatTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGt                                                                                        <  2:3182166/101‑1 (MQ=255)

        taatTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGt                                                                                        >  2:3182343/1‑101 (MQ=255)

         aatTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGtt                                                                                       <  2:3181899/101‑1 (MQ=255)

         aatTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGtt                                                                                       <  2:3182170/101‑1 (MQ=255)

          atTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTc                                                                                      >  2:3182347/1‑101 (MQ=255)

          atTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTc                                                                                      <  1:3181924/101‑1 (MQ=255)

            tttAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCAc                                                                                    >  1:3182349/1‑101 (MQ=255)

              tAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCt                                                                                  <  1:3182087/101‑1 (MQ=255)

              tAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCt                                                                                  >  2:3182351/1‑101 (MQ=255)

              tAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCt                                                                                  >  1:3182352/1‑101 (MQ=255)

               aggcccGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCg                                                                                         <  2:3181978/90‑1 (MQ=255)

                gTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTg                                                                                <  1:3182002/101‑1 (MQ=255)

                gTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTg                                                                                <  2:3181748/101‑1 (MQ=255)

                 tCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTgg                                                                               >  1:3182354/1‑101 (MQ=255)

                 tCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTgg                                                                               <  2:3182061/101‑1 (MQ=255)

                    cGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTc                                                                            >  1:3182357/1‑101 (MQ=255)

                      gtgtCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCaa                                                                          <  1:3182130/101‑1 (MQ=255)

                      gtgtCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCaa                                                                          <  1:3182117/101‑1 (MQ=255)

                       tgtCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAAc                                                                         >  1:3182359/1‑101 (MQ=255)

                        gtCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACa                                                                        <  1:3182006/101‑1 (MQ=255)

                         tCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACAt                                                                       <  2:3181913/101‑1 (MQ=255)

                         tCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACAt                                                                       <  2:3181936/101‑1 (MQ=255)

                          cGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATg                                                                      >  2:3182361/1‑101 (MQ=255)

                           gATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGc                                                                     >  1:3182363/1‑101 (MQ=255)

                           gATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTAAACATGc                                                                     <  1:3182151/101‑1 (MQ=255)

                            aTTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCg                                                                    >  1:3182365/1‑101 (MQ=255)

                              tCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGa                                                                  <  2:3181681/101‑1 (MQ=255)

                                cTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATa                                                                <  2:3181929/101‑1 (MQ=255)

                                cTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATa                                                                >  2:3182368/1‑101 (MQ=255)

                                 tACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAg                                                               >  1:3182371/1‑101 (MQ=255)

                                   ccTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCt                                                             >  2:3182372/1‑101 (MQ=255)

                                    cTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCtt                                                            >  1:3182375/1‑101 (MQ=255)

                                      cATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCa                                                          >  2:3182377/1‑101 (MQ=255)

                                      cATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCa                                                          <  2:3181998/101‑1 (MQ=255)

                                      cATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCa                                                          <  2:3182083/101‑1 (MQ=255)

                                       aTCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCAt                                                         >  1:3182379/1‑101 (MQ=255)

                                        tCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCAtt                                                        <  2:3182031/101‑1 (MQ=255)

                                         ccAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTa                                                       >  2:3182381/1‑101 (MQ=255)

                                         ccAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTa                                                       <  2:3182039/101‑1 (MQ=255)

                                          cAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTaa                                                      <  2:3181990/101‑1 (MQ=255)

                                           aaaaGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAAt                                                     >  1:3182383/1‑101 (MQ=255)

                                                ttGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAg                                                >  2:3182386/1‑101 (MQ=255)

                                                 tGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAgg                                               >  1:3182387/1‑101 (MQ=255)

                                                   gATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGgcg                                             <  2:3181860/101‑1 (MQ=255)

                                                    aTTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGgcgc                                            <  2:3182074/101‑1 (MQ=255)

                                                     ttGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTaa                                                 <  2:3182125/95‑1 (MQ=255)

                                                     ttGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCt                                           >  1:3182390/1‑101 (MQ=255)

                                                      tGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTa                                          <  1:3182075/101‑1 (MQ=255)

                                                       gggCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTaa                                         >  1:3182393/1‑101 (MQ=255)

                                                         gCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGa                                       >  2:3182394/1‑101 (MQ=255)

                                                          ccTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGAt                                      <  2:3181981/101‑1 (MQ=255)

                                                          ccTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGAt                                      <  2:3181959/101‑1 (MQ=255)

                                                           cTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTc                                                           <  1:3182046/79‑1 (MQ=255)

                                                            tACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGc                                    >  2:3182397/1‑101 (MQ=255)

                                                             aCCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCg                                   >  1:3182400/1‑101 (MQ=255)

                                                             aCCGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCg                                   >  2:3182399/1‑101 (MQ=255)

                                                              ccGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGa                                  >  2:3182403/1‑101 (MQ=255)

                                                               cGGAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGAt                                 <  1:3181900/101‑1 (MQ=255)

                                                                ggAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGgc                                              <  2:3182012/87‑1 (MQ=255)

                                                                ggAAAACCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGAtt                                <  2:3181986/101‑1 (MQ=255)

                                                                  aaaaCCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGa                              >  1:3182406/1‑101 (MQ=255)

                                                                  aaaaCCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGa                              >  2:3182405/1‑101 (MQ=255)

                                                                     aCCAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGt                           <  1:3182143/101‑1 (MQ=255)

                                                                      ccAAATTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTa                          >  1:3182410/1‑101 (MQ=255)

                                                                        aaaTTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTaaa                        >  2:3182413/1‑101 (MQ=255)

                                                                        aaaTTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTaaa                        >  1:3182412/1‑101 (MQ=255)

                                                                         aaTTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTaaaa                       <  1:3182096/101‑1 (MQ=255)

                                                                         aaTTTCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTaaaa                       <  2:3182152/101‑1 (MQ=255)

                                                                            ttCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAAcc                    <  2:3182034/101‑1 (MQ=255)

                                                                            ttCACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAAcc                    >  1:3182417/1‑101 (MQ=255)

                                                                              cACTAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCgg                  >  2:3182418/1‑101 (MQ=255)

                                                                                 tAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCtt               >  2:3182423/1‑101 (MQ=255)

                                                                                 tAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCtt               >  1:3182421/1‑101 (MQ=255)

                                                                                 tAGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCtt               <  2:3182099/101‑1 (MQ=255)

                                                                                  aGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCttt              >  1:3182425/1‑101 (MQ=255)

                                                                                  aGCGCGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCttt              <  1:3182160/101‑1 (MQ=255)

                                                                                   gcgcGGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTg             <  1:3182052/101‑1 (MQ=255)

                                                                                    cgcgGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGt            >  2:3182426/1‑101 (MQ=255)

                                                                                     gcgGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTc           >  1:3182429/1‑101 (MQ=255)

                                                                                      cgGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTca          <  2:3182118/101‑1 (MQ=255)

                                                                                      cgGCATCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTca          >  2:3182431/1‑101 (MQ=255)

                                                                                          aTCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTcac         <  2:3182092/98‑1 (MQ=255)

                                                                                           tCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTcacgcac     >  1:3182435/1‑101 (MQ=255)

                                                                                           tCAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTcacgcac     >  2:3182434/1‑101 (MQ=255)

                                                                                            cAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTcacgcacg    <  1:3182106/101‑1 (MQ=255)

                                                                                            cAATGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTcacgcacg    >  1:3182437/1‑101 (MQ=255)

                                                                                             aaTGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGa   >  1:3182440/1‑101 (MQ=255)

                                                                                              aTGCGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGAc  <  1:3182135/101‑1 (MQ=255)

TACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGAC  >  NC_000913/2457510‑2457704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`