BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,977,660	A→C	L414V (TTA→GTA)	lysA ←	diaminopimelate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,977,660	0	A	C	100.0%	1060.4 / NA	255	L414V (TTA→GTA)	lysA	diaminopimelate decarboxylase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (122/133); total (122/133)

Only 100 of 259 total aligned reads displayed.

ACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATT  >  NC_000913/2977562‑2977757

aCGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACtt                                                                                                 <  1:3854006/101‑1 (MQ=255)

 cGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACttc                                                                                                <  2:3853949/101‑1 (MQ=255)

  gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACttct                                                                                               >  1:3854402/1‑101 (MQ=255)

   cTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTActtctt                                                                                              >  2:3854403/1‑101 (MQ=255)

   cTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTActtctt                                                                                              <  2:3853968/101‑1 (MQ=255)

     ggTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCg                                                                                            <  2:3853836/101‑1 (MQ=255)

     ggTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCg                                                                                            >  2:3854409/1‑101 (MQ=255)

      gTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGa                                                                                           <  1:3853779/101‑1 (MQ=255)

       tACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGAt                                                                                          <  1:3853882/101‑1 (MQ=255)

       tACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGAt                                                                                          <  1:3853963/101‑1 (MQ=255)

       tACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGAt                                                                                          <  2:3854126/101‑1 (MQ=255)

       tACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGAt                                                                                          >  1:3854410/1‑101 (MQ=255)

        aCGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATg                                                                                         <  2:3853986/101‑1 (MQ=255)

           tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTc                                                                                      >  1:3854412/1‑101 (MQ=255)

            cgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCt                                                                                     <  2:3854131/101‑1 (MQ=255)

              tcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTgg                                                                                   >  2:3854418/1‑101 (MQ=255)

              tcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTgg                                                                                   >  1:3854417/1‑101 (MQ=255)

              tcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTgg                                                                                   >  2:3854421/1‑101 (MQ=255)

               cATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGc                                                                                  >  1:3854422/1‑101 (MQ=255)

                 ttCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGa                                                                                <  2:3854038/101‑1 (MQ=255)

                  tCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGAc                                                                               <  2:3854002/101‑1 (MQ=255)

                    aGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGa                                                                                <  2:3853893/98‑1 (MQ=255)

                    aGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACgg                                                                             <  1:3854064/101‑1 (MQ=255)

                    aGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACgg                                                                             <  1:3854210/101‑1 (MQ=255)

                     gtgtCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGc                                                                            >  1:3854425/1‑101 (MQ=255)

                         cAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAAt                                                                        >  1:3854428/1‑101 (MQ=255)

                         cAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAAt                                                                        <  1:3854024/101‑1 (MQ=255)

                          aaCTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATc                                                                       <  2:3853906/101‑1 (MQ=255)

                          aaCTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATc                                                                       <  2:3854135/101‑1 (MQ=255)

                           aCTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACg                                                                              >  1:3854431/1‑93 (MQ=255)

                           aCTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCa                                                                      <  2:3854192/101‑1 (MQ=255)

                            cTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAAt                                                                        <  2:3854111/98‑1 (MQ=255)

                              ccGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAAcc                                                                   <  2:3854076/101‑1 (MQ=255)

                               cGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAAc                                                                    <  1:3854147/99‑1 (MQ=255)

                                 tCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACcgcg                                                                <  2:3854308/101‑1 (MQ=255)

                                  cGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCgcgc                                                               >  2:3854434/1‑101 (MQ=255)

                                    cTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCt                                                             >  1:3854436/1‑101 (MQ=255)

                                     tGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTg                                                            <  1:3853863/101‑1 (MQ=255)

                                     tGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTg                                                            <  1:3853948/101‑1 (MQ=255)

                                      ggAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGa                                                           >  2:3854439/1‑101 (MQ=255)

                                      ggAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGa                                                           >  1:3854440/1‑101 (MQ=255)

                                        aGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGAcc                                                         <  1:3854073/101‑1 (MQ=255)

                                         ggCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCgcgc                                                               <  1:3853995/94‑1 (MQ=255)

                                          gCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCAt                                                       <  2:3854012/101‑1 (MQ=255)

                                          gCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCAt                                                       >  2:3854445/1‑101 (MQ=255)

                                           cAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCAtt                                                      <  1:3854117/101‑1 (MQ=255)

                                            aaGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTa                                                     >  2:3854447/1‑101 (MQ=255)

                                              gTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATc                                                   >  2:3854450/1‑101 (MQ=255)

                                               tcatcaTGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCa                                                  >  1:3854451/1‑101 (MQ=255)

                                                 atcatGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCt                                                                                     <  2:3854158/64‑1 (MQ=255)

                                                 atcatGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCaaa                                                >  1:3854456/1‑101 (MQ=255)

                                                  tcatGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGaa                                                                         >  2:3854457/1‑75 (MQ=255)

                                                   catGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACa                                              >  2:3854459/1‑101 (MQ=255)

                                                    atGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAg                                             >  1:3854462/1‑101 (MQ=255)

                                                    atGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAg                                             <  2:3854113/101‑1 (MQ=255)

                                                     tGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGa                                            <  2:3854029/101‑1 (MQ=255)

                                                          ccAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTc                                       >  2:3854466/1‑101 (MQ=255)

                                                           cAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCt                                      >  1:3854468/1‑101 (MQ=255)

                                                           cAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCt                                      <  1:3853972/101‑1 (MQ=255)

                                                            aGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTg                                     >  2:3854472/1‑101 (MQ=255)

                                                            aGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTg                                     >  1:3854474/1‑101 (MQ=255)

                                                            aGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTg                                     >  1:3854470/1‑101 (MQ=255)

                                                               gACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTa                                  >  2:3854477/1‑101 (MQ=255)

                                                                aCTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTaa                                 <  1:3854250/101‑1 (MQ=255)

                                                                aCTAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTaa                                 <  2:3854053/101‑1 (MQ=255)

                                                                  tAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACa                               >  2:3854482/1‑101 (MQ=255)

                                                                  tAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACa                               >  1:3854480/1‑101 (MQ=255)

                                                                  tAACCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACa                               <  1:3854331/101‑1 (MQ=255)

                                                                   aaCCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAg                              >  2:3854486/1‑101 (MQ=255)

                                                                    aCCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGc                             >  2:3854489/1‑101 (MQ=255)

                                                                    aCCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGc                             >  2:3854491/1‑101 (MQ=255)

                                                                    aCCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGc                             <  1:3854039/101‑1 (MQ=255)

                                                                    aCCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGc                             <  1:3854051/101‑1 (MQ=255)

                                                                    aCCGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGc                             <  2:3853916/101‑1 (MQ=255)

                                                                     ccGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCg                            <  2:3854093/101‑1 (MQ=255)

                                                                     ccGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCg                            >  1:3854492/1‑101 (MQ=255)

                                                                      cGCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCgg                           <  1:3854291/101‑1 (MQ=255)

                                                                       gCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGa                          <  2:3854169/101‑1 (MQ=255)

                                                                       gCAGTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGa                          <  1:3854086/101‑1 (MQ=255)

                                                                          gTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACgg                       >  1:3854498/1‑101 (MQ=255)

                                                                          gTTAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACgg                       >  2:3854497/1‑101 (MQ=255)

                                                                           ttAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGc                      >  1:3854501/1‑101 (MQ=255)

                                                                            tAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCt                     <  1:3854031/101‑1 (MQ=255)

                                                                            tAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCt                     <  1:3854048/101‑1 (MQ=255)

                                                                            tAAAGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCt                     <  1:3854162/101‑1 (MQ=255)

                                                                              aaGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTAt                   >  2:3854505/1‑101 (MQ=255)

                                                                              aaGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTAt                   <  1:3854236/101‑1 (MQ=255)

                                                                              aaGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTAt                   <  1:3854159/101‑1 (MQ=255)

                                                                               aGCAATTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTAtt                  >  1:3854506/1‑101 (MQ=255)

                                                                                  aaTTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTa               >  2:3854508/1‑101 (MQ=255)

                                                                                   aTTCCAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAg              >  2:3854511/1‑101 (MQ=255)

                                                                                      ccAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTg           >  2:3854514/1‑101 (MQ=255)

                                                                                       cAGCGCCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTgg          >  1:3854515/1‑101 (MQ=255)

                                                                                         gcgcCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGAt        >  2:3854518/1‑101 (MQ=255)

                                                                                         gcgcCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGAt        <  2:3854217/101‑1 (MQ=255)

                                                                                         gcgcCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGAt        <  1:3854193/101‑1 (MQ=255)

                                                                                         gcgcCAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGAt        <  1:3854092/101‑1 (MQ=255)

                                                                                            ccAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTa                                  >  1:3854522/1‑72 (MQ=255)

                                                                                             cAGTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACa    <  2:3854228/101‑1 (MQ=255)

                                                                                               gTACTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACAtt  <  1:3853919/101‑1 (MQ=255)

ACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATT  >  NC_000913/2977562‑2977757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`