BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,310,748	A→G	S141P (TCT→CCT)	pnp ←	polynucleotide phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,310,748	0	A	G	100.0%	908.3 / NA	220	S141P (TCT→CCT)	pnp	polynucleotide phosphorylase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (117/103); total (117/103)

Only 100 of 242 total aligned reads displayed.

GCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGCGATAACCTGAACTTCGTTG  >  NC_000913/3310650‑3310845

     ctTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCaggcagagac                                                                                            >  1:4315308/1‑101 (MQ=255)

      tttCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCaggcagagaca                                                                                           >  2:4315309/1‑101 (MQ=255)

       ttCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCaggcagagacag                                                                                          >  2:4315314/1‑101 (MQ=255)

        tCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAgc                                                                                         <  1:4314761/101‑1 (MQ=255)

        tCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAgc                                                                                         >  1:4315315/1‑101 (MQ=255)

        tCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAgc                                                                                         >  1:4315317/1‑101 (MQ=255)

         cAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAgcg                                                                                        <  2:4315029/101‑1 (MQ=255)

          aGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAgcgc                                                                                       <  1:4314891/101‑1 (MQ=255)

           gCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCt                                                                                      >  2:4315321/1‑101 (MQ=255)

            cTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTg                                                                                     >  1:4315323/1‑101 (MQ=255)

            cTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTg                                                                                     <  2:4314948/101‑1 (MQ=255)

                tCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGa                                                                                 >  1:4315328/1‑101 (MQ=255)

                 ccTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGaa                                                                                >  2:4315329/1‑101 (MQ=255)

                    gAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCa                                                                             >  2:4315332/1‑101 (MQ=255)

                    gAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCa                                                                             <  1:4314901/101‑1 (MQ=255)

                    gAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCa                                                                             <  2:4314903/101‑1 (MQ=255)

                     aGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCAc                                                                            >  2:4315335/1‑101 (MQ=255)

                     aGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCAc                                                                            >  1:4315333/1‑101 (MQ=255)

                     aGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACAGAATACCAGGCAGAGACAGCGCTGCGGAAGCAc                                                                            <  2:4314961/101‑1 (MQ=255)

                      gTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCAcc                                                                           <  1:4315067/101‑1 (MQ=255)

                      gTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCAcc                                                                           <  1:4315009/101‑1 (MQ=255)

                        cGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCa                                                                          >  2:4315338/1‑100 (MQ=255)

                         gggTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAat                                                                        <  1:4315266/101‑1 (MQ=255)

                            ttCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAatcat                                                                     <  2:4314919/101‑1 (MQ=255)

                            ttCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAatcat                                                                     >  1:4315341/1‑101 (MQ=255)

                             tCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCTATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAatcatc                                                                    >  2:4315343/1‑101 (MQ=255)

                              cAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATcg                                                                   <  2:4314967/101‑1 (MQ=255)

                                 tacgtacTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGcga                                                                >  1:4315347/1‑101 (MQ=255)

                                 tacgtacTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGcga                                                                <  2:4315091/101‑1 (MQ=255)

                                  acgtacTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGcgac                                                               >  2:4315348/1‑101 (MQ=255)

                                  acgtacTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGcgac                                                               <  1:4314992/101‑1 (MQ=255)

                                   cgtacTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGcgacg                                                              >  2:4315350/1‑101 (MQ=255)

                                    gtacTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGcgacga                                                             <  1:4314960/101‑1 (MQ=255)

                                     tacTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGat                                                            <  1:4314923/101‑1 (MQ=255)

                                      acTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGata                                                           <  1:4314944/101‑1 (MQ=255)

                                      acTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGata                                                           >  2:4315355/1‑101 (MQ=255)

                                      acTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGata                                                           >  1:4315354/1‑101 (MQ=255)

                                        tGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATc                                                         <  1:4315054/101‑1 (MQ=255)

                                          gTCATTGATGGAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCg                                                       >  2:4315358/1‑101 (MQ=255)

                                           tCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCgg                                                      <  1:4314834/101‑1 (MQ=255)

                                           tCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCgg                                                      <  1:4315002/101‑1 (MQ=255)

                                           tCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCgg                                                      <  1:4315049/101‑1 (MQ=255)

                                            cATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCggg                                                     >  1:4315360/1‑101 (MQ=255)

                                             aTTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGt                                                    >  2:4315362/1‑101 (MQ=255)

                                              ttGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGtt                                                   <  1:4314851/101‑1 (MQ=255)

                                               tGATGTAACCTACGCGTGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTa                                                  >  1:4315363/1‑101 (MQ=255)

                                               tGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTa                                                  <  2:4315020/101‑1 (MQ=255)

                                                gATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTaa                                                 <  2:4314845/101‑1 (MQ=255)

                                                  tGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGcgac                                                               >  2:4315367/1‑85 (MQ=255)

                                                   gTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGt                                                    >  1:4315369/1‑95 (MQ=255)

                                                    tAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCa                                                                          >  2:4315371/1‑72 (MQ=255)

                                                     aaCCTGCGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGc                                            >  1:4315373/1‑101 (MQ=255)

                                                       ccTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCgg                                          >  1:4315377/1‑101 (MQ=255)

                                                        cTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCggg                                         >  1:4315380/1‑101 (MQ=255)

                                                         tACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGt                                        <  2:4315098/101‑1 (MQ=255)

                                                         tACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGt                                        >  2:4315382/1‑101 (MQ=255)

                                                          aCGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGtt                                       <  1:4314843/101‑1 (MQ=255)

                                                           cgcgGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTa                                      <  1:4315105/101‑1 (MQ=255)

                                                           cgcgGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTa                                      >  2:4315385/1‑101 (MQ=255)

                                                            gcgGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTaa                                     <  1:4315099/101‑1 (MQ=255)

                                                            gcgGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTaa                                     <  1:4315042/101‑1 (MQ=255)

                                                            gcgGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTaa                                     >  2:4315388/1‑101 (MQ=255)

                                                            gcgGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTaa                                     >  2:4315391/1‑101 (MQ=255)

                                                            gcgGGCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTaa                                     >  1:4315387/1‑101 (MQ=255)

                                                              gggCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACa                                   >  1:4315394/1‑101 (MQ=255)

                                                               ggCAGCACCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAg                                  <  2:4314999/101‑1 (MQ=255)

                                                                gcagcaCCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGGTAACTTGCGGGTTAACAGa                                 >  2:4315396/1‑101 (MQ=255)

                                                                  agcaCCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGaaa                               >  1:4315398/1‑101 (MQ=255)

                                                                   gcaCCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAAc                              <  1:4315082/101‑1 (MQ=255)

                                                                   gcaCCAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAAc                              <  2:4315068/101‑1 (MQ=255)

                                                                      ccAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGtt                                       <  2:4315302/89‑1 (MQ=255)

                                                                       cAATCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACg                          >  2:4315402/1‑101 (MQ=255)

                                                                        aaTCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACgg                         <  2:4315154/101‑1 (MQ=255)

                                                                        aaTCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACgg                         >  2:4315404/1‑101 (MQ=255)

                                                                         aTCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGt                        >  2:4315407/1‑101 (MQ=255)

                                                                          tCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTg                       >  2:4315410/1‑101 (MQ=255)

                                                                          tCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTg                       >  1:4315409/1‑101 (MQ=255)

                                                                          tCGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTg                       <  1:4315149/101‑1 (MQ=255)

                                                                           cGGGCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACgg                         >  1:4315412/1‑98 (MQ=255)

                                                                            gggCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGc                     <  1:4314870/101‑1 (MQ=255)

                                                                            gggCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGc                     <  1:4315163/101‑1 (MQ=255)

                                                                             ggCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGCg                    <  1:4315034/101‑1 (MQ=255)

                                                                              gCCATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGCGa                   <  2:4314936/101‑1 (MQ=255)

                                                                                cATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGCGATa                 >  2:4315417/1‑101 (MQ=255)

                                                                                cATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGCGATa                 <  1:4315016/101‑1 (MQ=255)

                                                                                cATTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGCGATa                 >  1:4315416/1‑101 (MQ=255)

                                                                                 aTTGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGACATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGCGATaa                <  2:4315046/101‑1 (MQ=255)

                                                                                  ttGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGCGATAAc               <  1:4315024/101‑1 (MQ=255)

                                                                                   tGAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGCGATAAcc              >  1:4315421/1‑101 (MQ=255)

                                                                                    gAACGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGCGATAACCt             <  1:4314980/101‑1 (MQ=255)

                                                                                     aaCGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGCGATAACCTg            >  2:4315422/1‑101 (MQ=255)

                                                                                       cGGAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGCGATAACCTGaa          <  1:4315134/101‑1 (MQ=255)

                                                                                        ggAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGCGATAACCTGAAc         >  2:4315427/1‑101 (MQ=255)

                                                                                        ggAATACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGCGATAACCTGAAc         >  1:4315426/1‑101 (MQ=255)

                                                                                           aTACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCggg                                                     >  1:4315431/1‑54 (MQ=255)

                                                                                            tACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGCGATAACCTGAACTTCg     <  2:4314984/101‑1 (MQ=255)

                                                                                            tACCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGCGATAACCTGAACTTCg     >  2:4315433/1‑101 (MQ=255)

                                                                                             aCCAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGCGATAACCTGAACTTCGt    >  2:4315435/1‑101 (MQ=255)

                                                                                              ccAGGCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGCGATAACCTGAACTTCGtt   <  2:4315083/101‑1 (MQ=255)

                                                                                               caggcagaGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGCGATAACCTGAACTTCGTTg  >  1:4315438/1‑101 (MQ=255)

GCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGCGATAACCTGAACTTCGTTG  >  NC_000913/3310650‑3310845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`