BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,372,795	T→C	Y261C (TAT→TGT)	nanA ←	N‑acetylneuraminate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,372,795	0	T	C	100.0%	1119.3 / NA	271	Y261C (TAT→TGT)	nanA	N‑acetylneuraminate lyase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (100/171); total (100/171)

Only 100 of 275 total aligned reads displayed.

GCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCAGTTTCTGCGCGGTCTGGAT  >  NC_000913/3372697‑3372892

gCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACaa                                                                                                 <  2:4399499/101‑1 (MQ=255)

gCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACaa                                                                                                 <  1:4399531/101‑1 (MQ=255)

 cATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAAt                                                                                                >  2:4399916/1‑101 (MQ=255)

 cATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAAt                                                                                                <  2:4399607/101‑1 (MQ=255)

  aTCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATg                                                                                               >  1:4399918/1‑101 (MQ=255)

     aaCTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATggag                                                                                            >  2:4399922/1‑101 (MQ=255)

         gctgGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACa                                                                                        >  1:4399923/1‑101 (MQ=255)

         gctgGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACa                                                                                        <  2:4399666/101‑1 (MQ=255)

            gggCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGtt                                                                                     <  2:4399630/101‑1 (MQ=255)

               ccAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTc                                                                                  <  2:4399444/101‑1 (MQ=255)

                cAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCa                                                                                 >  1:4399926/1‑101 (MQ=255)

                 aGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAg                                                                                <  2:4399762/101‑1 (MQ=255)

                    gcCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGcc                                                                             >  2:4399931/1‑101 (MQ=255)

                    gcCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGcc                                                                             >  1:4399930/1‑101 (MQ=255)

                    gcCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGcc                                                                             <  1:4399371/101‑1 (MQ=255)

                     ccTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCcg                                                                            <  2:4399739/101‑1 (MQ=255)

                     ccTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCcg                                                                            <  2:4399305/101‑1 (MQ=255)

                        tCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCgg                                                                         >  2:4399934/1‑101 (MQ=255)

                          aGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGaa                                                                       >  1:4399938/1‑101 (MQ=255)

                          aGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGaa                                                                       <  1:4399479/101‑1 (MQ=255)

                           gTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAAt                                                                      <  1:4399519/101‑1 (MQ=255)

                           gTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAAt                                                                      <  1:4399634/101‑1 (MQ=255)

                            ttCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATa                                                                     <  1:4399450/101‑1 (MQ=255)

                             tCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATAc                                                                    <  1:4399708/101‑1 (MQ=255)

                                 gCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGaa                                                                       <  1:4399408/94‑1 (MQ=255)

                                  cAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCg                                                               >  1:4399940/1‑101 (MQ=255)

                                   aGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGt                                                              <  2:4399403/101‑1 (MQ=255)

                                    gATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGtt                                                             <  1:4399507/101‑1 (MQ=255)

                                     atatTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGttt                                                            <  1:4399855/101‑1 (MQ=255)

                                     atatTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGttt                                                            <  2:4399577/101‑1 (MQ=255)

                                     atatTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGttt                                                            >  2:4399942/1‑101 (MQ=255)

                                      tatTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGtttt                                                           <  1:4399548/101‑1 (MQ=255)

                                       atTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTg                                                          <  1:4399598/101‑1 (MQ=255)

                                       atTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTg                                                          <  1:4399663/101‑1 (MQ=255)

                                       atTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTg                                                          <  2:4399480/101‑1 (MQ=255)

                                        tttttCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGa                                                         <  1:4399538/101‑1 (MQ=255)

                                        tttttCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGa                                                         >  2:4399947/1‑101 (MQ=255)

                                         ttttCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGAt                                                        <  1:4399622/101‑1 (MQ=255)

                                          tttCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATc                                                       >  1:4399950/1‑101 (MQ=255)

                                          tttCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATc                                                       <  2:4399807/101‑1 (MQ=255)

                                          tttCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTCTTCAGGCCGCGGAATACGCCCGTTTTGATc                                                       >  2:4399949/1‑101 (MQ=255)

                                            tCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAg                                                     <  1:4399711/101‑1 (MQ=255)

                                             cATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGt                                                    <  2:4399586/101‑1 (MQ=255)

                                              aTCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTa                                                   <  1:4399697/101‑1 (MQ=255)

                                               tCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTaa                                                  >  2:4399957/1‑101 (MQ=255)

                                               tCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTaa                                                  >  1:4399955/1‑101 (MQ=255)

                                                cTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTaaa                                                 <  1:4399679/101‑1 (MQ=255)

                                                 tACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAAt                                                >  2:4399961/1‑101 (MQ=255)

                                                  aCCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTa                                                   <  2:4399544/97‑1 (MQ=255)

                                                  aCCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATc                                               >  1:4399962/1‑101 (MQ=255)

                                                   ccGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCa                                              <  2:4399686/101‑1 (MQ=255)

                                                     ggTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAAt                                            >  2:4399968/1‑101 (MQ=255)

                                                     ggTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAAt                                            >  1:4399966/1‑101 (MQ=255)

                                                      gTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATg                                           >  2:4399970/1‑101 (MQ=255)

                                                       tCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGa                                          <  2:4399514/101‑1 (MQ=255)

                                                       tCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGa                                          <  2:4399427/101‑1 (MQ=255)

                                                        ccAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGAc                                         >  1:4399972/1‑101 (MQ=255)

                                                         cAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACt                                        >  1:4399976/1‑101 (MQ=255)

                                                          aaaCGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACtt                                       <  2:4399364/101‑1 (MQ=255)

                                                           aaCGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACttt                                      <  1:4399716/101‑1 (MQ=255)

                                                            aCGGTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAACTCAATGACTTTa                                     >  1:4399980/1‑101 (MQ=255)

                                                              ggTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTAtt                                   >  1:4399982/1‑101 (MQ=255)

                                                              ggTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTAtt                                   >  2:4399984/1‑101 (MQ=255)

                                                              ggTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTAtt                                   <  1:4399356/101‑1 (MQ=255)

                                                               gTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATTAATGACTTTATTg                                  <  1:4399643/101‑1 (MQ=255)

                                                               gTTTGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTg                                  <  2:4399614/101‑1 (MQ=255)

                                                                tttGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGc                                 <  2:4399705/101‑1 (MQ=255)

                                                                 ttGCGGCACAGCGGCACAGAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCa                                >  1:4399988/1‑101 (MQ=255)

                                                                    cggcacagcggcacagAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTc                             >  2:4399990/1‑101 (MQ=255)

                                                                    cggcacagcggcacagAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTc                             <  2:4399675/101‑1 (MQ=255)

                                                                     ggcacagcggcacagAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAAt                                                <  1:4399615/81‑1 (MQ=255)

                                                                     ggcacagcggcacagAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCa                            <  2:4399747/101‑1 (MQ=255)

                                                                     ggcacagcggcacagAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCa                            <  1:4399767/101‑1 (MQ=255)

                                                                      gcacagcggcacagAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAg                           >  1:4399993/1‑101 (MQ=255)

                                                                        acagcggcacagAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGtt                         >  2:4399997/1‑101 (MQ=255)

                                                                        acagcggcacagAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGtt                         <  2:4399815/101‑1 (MQ=255)

                                                                         cagcggcacagAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGttt                        <  2:4399694/101‑1 (MQ=255)

                                                                         cagcggcacagAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGttt                        >  1:4399998/1‑101 (MQ=255)

                                                                           gcggcacagAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGc                      <  2:4399452/101‑1 (MQ=255)

                                                                           gcggcacagAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGc                      >  1:4400002/1‑101 (MQ=255)

                                                                           gcggcacagAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGc                      >  2:4400001/1‑101 (MQ=255)

                                                                            cggcacagAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCa                     <  1:4399704/101‑1 (MQ=255)

                                                                            cggcacagAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCa                     <  1:4399652/101‑1 (MQ=255)

                                                                            cggcacagAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCa                     <  1:4399596/101‑1 (MQ=255)

                                                                             ggcacagAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCAg                    <  2:4399804/101‑1 (MQ=255)

                                                                             ggcacagAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCAg                    <  2:4399527/101‑1 (MQ=255)

                                                                              gcacagAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCa                     <  1:4399898/99‑1 (MQ=255)

                                                                              gcacagAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCAGt                   <  2:4399594/101‑1 (MQ=255)

                                                                               cacagAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCAGtt                  >  2:4400007/1‑101 (MQ=255)

                                                                                acagAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCAGttt                 <  1:4399722/101‑1 (MQ=255)

                                                                                 cagAAACGAAATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCAGTTTc                >  1:4400008/1‑101 (MQ=255)

                                                                                  agAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCAGTTTCt               <  2:4399567/101‑1 (MQ=255)

                                                                                  agAAACGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCAGTTTCt               <  1:4399749/101‑1 (MQ=255)

                                                                                    aaaCGACATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCAGTTTCTgc             <  1:4399579/101‑1 (MQ=255)

                                                                                         aCATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCAGTTTCTGCGCGGt        <  2:4399573/101‑1 (MQ=255)

                                                                                          cATCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCAGTTTCTGCGCGGTc       <  2:4399777/101‑1 (MQ=255)

                                                                                           aTCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCAGTTTCTGCGCGGTCt      >  2:4400013/1‑101 (MQ=255)

                                                                                            tCCATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCAGTTTCTGCGCGGTCTg     <  1:4399671/101‑1 (MQ=255)

                                                                                              cATACAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCAGTTTCTg              >  1:4400015/1‑90 (MQ=255)

                                                                                               atacAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCAGTTTCTGCGCGGTCTGGat  <  1:4399487/101‑1 (MQ=255)

GCATCAACTGCTGGGCCAGCGCCTTCAGTTCTGGCAGATATTTTTCATCTACCGGTCCAAACGGTTTGCGGCACAGCGGCACAGAAACGACATCCATATAATGGAGGACAGTTTTCAGGCCGCGGAATACGCCCGTTTTGATCAGTAAATCAATGACTTTATTGCATTCAGTTTGCAGTTTCTGCGCGGTCTGGAT  >  NC_000913/3372697‑3372892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`