Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,521,219 T→C P77P (CCA→CCG)  hofO ← DNA utilization protein HofO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,521,2190TC100.0% 990.8 / NA 241P77P (CCA→CCG) hofODNA utilization protein HofO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (120/121);  total (120/121)

Only 100 of 243 total aligned reads displayed.

GACGGCACTGCTTCCCACAGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGTGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCACTGCTGGTGATGACTTGCCCGTAGTCGAA  >  NC_000913/3521121‑3521316
                                                                                                  |                                                                                                 
gACGGCACTGCTTCCCACAGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCgg                                                                                                 <  1:4597275/101‑1 (MQ=255)
 aCGGCACTGCTTCCCACAGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGc                                                                                                >  1:4597738/1‑101 (MQ=255)
    gCACTGCTTCCCACAGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGaa                                                                                             <  2:4597576/101‑1 (MQ=255)
     cACTGCTTCCCACAGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGaaa                                                                                            >  1:4597741/1‑101 (MQ=255)
       cTGCTTCCCACAGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCgg                                                                                                 <  1:4597349/94‑1 (MQ=255)
       cTGCTTCCCACAGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAg                                                                                          >  1:4597745/1‑101 (MQ=255)
       cTGCTTCCCACAGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAg                                                                                          >  2:4597747/1‑101 (MQ=255)
        tGCTTCCCACAGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAgg                                                                                         >  1:4597749/1‑101 (MQ=255)
        tGCTTCCCACAGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAgg                                                                                         >  2:4597751/1‑101 (MQ=255)
        tGCTTCCCACAGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAgg                                                                                         <  2:4597558/101‑1 (MQ=255)
         gCTTCCCACAGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGaa                                                                                             >  1:4597752/1‑96 (MQ=255)
         gCTTCCCACAGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAggg                                                                                        <  1:4597568/101‑1 (MQ=255)
         gCTTCCCACAGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAggg                                                                                        <  2:4597541/101‑1 (MQ=255)
         gCTTCCCACAGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAggg                                                                                        <  2:4597341/101‑1 (MQ=255)
           ttCCCACAGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCa                                                                                      >  1:4597755/1‑101 (MQ=255)
             cccACAGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGc                                                                                    <  2:4596959/101‑1 (MQ=255)
             cccACAGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCAGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGc                                                                                    <  1:4597416/101‑1 (MQ=255)
               cacaGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGt                                                                                  <  1:4597290/101‑1 (MQ=255)
                acaGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGtt                                                                                 <  1:4597358/101‑1 (MQ=255)
                 caGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGttt                                                                                <  2:4597394/101‑1 (MQ=255)
                  aGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGtttt                                                                               >  1:4597758/1‑101 (MQ=255)
                  aGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGtttt                                                                               <  2:4597356/101‑1 (MQ=255)
                    cGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGtttttt                                                                             >  2:4597761/1‑101 (MQ=255)
                     gTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTc                                                                            >  2:4597763/1‑101 (MQ=255)
                      ttttCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTcc                                                                           >  2:4597767/1‑101 (MQ=255)
                       tttCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGtttttt                                                                             <  1:4597615/98‑1 (MQ=255)
                       tttCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCt                                                                          <  1:4597466/101‑1 (MQ=255)
                        ttCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTc                                                                         <  2:4597370/101‑1 (MQ=255)
                        ttCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTc                                                                         <  2:4597442/101‑1 (MQ=255)
                        ttCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTc                                                                         <  1:4597360/101‑1 (MQ=255)
                         tCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCg                                                                        >  2:4597769/1‑101 (MQ=255)
                           aaCGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCt                                                                      >  1:4597771/1‑101 (MQ=255)
                            aCGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTg                                                                     <  1:4597404/101‑1 (MQ=255)
                            aCGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTg                                                                     >  2:4597774/1‑101 (MQ=255)
                            aCGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTg                                                                     <  2:4597448/101‑1 (MQ=255)
                             cGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGa                                                                    <  1:4597396/101‑1 (MQ=255)
                             cGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGa                                                                    <  2:4597454/101‑1 (MQ=255)
                              gCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGaa                                                                   >  1:4597776/1‑101 (MQ=255)
                              gCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGaa                                                                   >  1:4597779/1‑101 (MQ=255)
                               ccAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAg                                                                  >  2:4597780/1‑101 (MQ=255)
                                cAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAgg                                                                 <  2:4597378/101‑1 (MQ=255)
                                  aCTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGa                                                               <  2:4597313/101‑1 (MQ=255)
                                   cTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGtt                                                                                 >  2:4597784/1‑82 (MQ=255)
                                   cTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAg                                                              >  1:4597783/1‑101 (MQ=255)
                                    tCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGc                                                             >  1:4597786/1‑101 (MQ=255)
                                      gCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCgg                                                           <  2:4597512/101‑1 (MQ=255)
                                       ccTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGgt                                                          >  1:4597790/1‑101 (MQ=255)
                                        cTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGgtg                                                         >  2:4597792/1‑101 (MQ=255)
                                         tCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGgtgt                                                        <  1:4597333/101‑1 (MQ=255)
                                           ccTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCt                                                      <  1:4597524/101‑1 (MQ=255)
                                            cTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTa                                                     >  2:4597795/1‑101 (MQ=255)
                                            cTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTa                                                     <  2:4597403/101‑1 (MQ=255)
                                            cTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTa                                                     >  1:4597796/1‑101 (MQ=255)
                                              gcgcGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTAc                                                    >  1:4597801/1‑100 (MQ=255)
                                               cgcgGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCa                                                  >  2:4597802/1‑101 (MQ=255)
                                                gcgGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAg                                                 <  2:4597288/101‑1 (MQ=255)
                                                 cgGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAgg                                                <  1:4597488/101‑1 (MQ=255)
                                                  ggatggatGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGc                                               <  2:4597421/101‑1 (MQ=255)
                                                   gatggatgCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGaa                                                                   <  1:4597338/80‑1 (MQ=255)
                                                    atggatgCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGa                                             >  1:4597804/1‑101 (MQ=255)
                                                     tggatgCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGAt                                            >  2:4597807/1‑101 (MQ=255)
                                                        atgCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCg                                                            >  1:4597810/1‑82 (MQ=255)
                                                         tgCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAg                                        <  1:4597264/101‑1 (MQ=255)
                                                         tgCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAg                                        >  2:4597812/1‑101 (MQ=255)
                                                           ccAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTg                                      >  2:4597815/1‑101 (MQ=255)
                                                           ccAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTg                                      >  1:4597816/1‑101 (MQ=255)
                                                            cAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGc                                     >  1:4597818/1‑101 (MQ=255)
                                                            cAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGc                                     <  1:4597374/101‑1 (MQ=255)
                                                            cAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGc                                     >  2:4597820/1‑101 (MQ=255)
                                                              ggAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCgg                                   <  2:4597464/101‑1 (MQ=255)
                                                                 aaCCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGccc                                <  2:4597327/101‑1 (MQ=255)
                                                                  aCCAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCa                               <  1:4597380/101‑1 (MQ=255)
                                                                    cAGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCgg                                   <  1:4597314/95‑1 (MQ=255)
                                                                     aGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCActg                            >  2:4597825/1‑101 (MQ=255)
                                                                     aGTTGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCActg                            >  1:4597826/1‑101 (MQ=255)
                                                                       ttGCGCGCCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCActgct                          <  1:4597505/101‑1 (MQ=255)
                                                                         gcgcgcCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCACTGCTgg                        >  2:4597829/1‑101 (MQ=255)
                                                                         gcgcgcCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCACTGCTgg                        <  1:4597311/101‑1 (MQ=255)
                                                                          cgcgcCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCACTGCTGGt                       <  1:4597435/101‑1 (MQ=255)
                                                                           gcgcCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCACTGCTGGtg                      <  1:4597458/101‑1 (MQ=255)
                                                                             gcCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAgg                                                                 >  2:4597833/1‑56 (MQ=255)
                                                                             gcCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGc                                     <  2:4597300/84‑1 (MQ=255)
                                                                             gcCGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCACTGCTGGtgat                    >  1:4597832/1‑101 (MQ=255)
                                                                              ccGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCt                                                                      <  2:4597283/50‑1 (MQ=255)
                                                                              ccGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCACTGCTGGtgatg                   <  2:4597204/101‑1 (MQ=255)
                                                                              ccGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCACTGCTGGtgatg                   <  2:4597428/101‑1 (MQ=255)
                                                                               cGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCACTGCTGGtgatga                  <  2:4597410/101‑1 (MQ=255)
                                                                               cGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCACTGCTGGtgatga                  <  1:4597439/101‑1 (MQ=255)
                                                                               cGGATAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCACTGCTGGtgatga                  >  2:4597835/1‑101 (MQ=255)
                                                                                  aTAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCACTGCTGGTGATGACtt               >  1:4597838/1‑101 (MQ=255)
                                                                                   tAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCACTGCTGGTGATGACTTg              <  1:4597493/101‑1 (MQ=255)
                                                                                   tAACTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCACTGCTGGTGATGACTTg              >  2:4597841/1‑101 (MQ=255)
                                                                                    aaCTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCACTGCTGGTGATGACTTGc             >  2:4597844/1‑101 (MQ=255)
                                                                                      cTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCACTGCTGGTGATGACTTGccc           <  2:4597652/101‑1 (MQ=255)
                                                                                      cTGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCACTGCTGGTGATGACTTGccc           >  1:4597845/1‑101 (MQ=255)
                                                                                       tGAAAATCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGCCTACCAGGCGATACAGTGCGGCCCACTGCTGGTGATGACTTGCCCg          <  1:4597669/101‑1 (MQ=255)
                                                                                           aaTCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCACTGCTGGTGATGACTTGCCCGTAGt      >  1:4597851/1‑101 (MQ=255)
                                                                                           aaTCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCACTGCTGGTGATGACTTGCCCGTAGt      >  1:4597849/1‑101 (MQ=255)
                                                                                            aTCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCACTGCTGGTGATGACTTGCCCGTAGTc     <  2:4597418/101‑1 (MQ=255)
                                                                                            aTCCAGCGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCACTGCTGGTGATGACTTGCCCGTAGTc     >  1:4597853/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GACGGCACTGCTTCCCACAGCGTTTTCAACGCCAACTCGCCTCCCTGCGCGGATGGATGCCAGGAAACCAGTTGCGCGCCGGATAACTGAAAATCCAGTGGCGAAAAGGGCAGCGTTTTTTCCTCGCTGAAGGGAGCGGTGTCTACCAGGCGATACAGTGCGGCCCACTGCTGGTGATGACTTGCCCGTAGTCGAA  >  NC_000913/3521121‑3521316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑