Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,596,545 C→T G339E (GGG→GAG)  livK ← L‑leucine/L‑phenylalanine ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,596,5450CT99.6% 987.1 / ‑4.3 237G339E (GGG→GAG) livKL‑leucine/L‑phenylalanine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (122/114);  total (122/115)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.33e-01

Only 100 of 237 total aligned reads displayed.

ATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGCAGTCGCCAGAGATTGCAC  >  NC_000913/3596447‑3596642
                                                                                                  |                                                                                                 
atgatCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCa                                                                                                 <  2:4699926/101‑1 (MQ=255)
atgatCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCa                                                                                                 <  2:4700386/101‑1 (MQ=255)
   atCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATc                                                                                              >  1:4700744/1‑101 (MQ=255)
    tCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCa                                                                                             >  2:4700745/1‑101 (MQ=255)
     cACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCAc                                                                                            >  1:4700748/1‑101 (MQ=255)
       cTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACgg                                                                                          <  2:4700173/101‑1 (MQ=255)
       cTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACgg                                                                                          >  1:4700751/1‑101 (MQ=255)
        ttGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGgt                                                                                         <  1:4700303/101‑1 (MQ=255)
           gCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGtt                                                                                      <  1:4700113/101‑1 (MQ=255)
            cTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGttt                                                                                     <  1:4700616/101‑1 (MQ=255)
            cTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGttt                                                                                     >  2:4700754/1‑101 (MQ=255)
             tGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTg                                                                                    <  2:4700432/101‑1 (MQ=255)
             tGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTg                                                                                    >  2:4700757/1‑101 (MQ=255)
             tGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTg                                                                                    <  1:4700232/101‑1 (MQ=255)
              gCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGc                                                                                   <  1:4700375/101‑1 (MQ=255)
              gCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGc                                                                                   <  1:4700466/101‑1 (MQ=255)
               ccGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCa                                                                                  >  1:4700760/1‑101 (MQ=255)
                cGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGgtgt                                                                                       <  1:4700337/95‑1 (MQ=255)
                 gTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGTTCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCCCGGTGTTTGCAcc                                                                                >  2:4700763/1‑101 (MQ=255)
                 gTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCAcc                                                                                >  1:4700762/1‑101 (MQ=255)
                   ggATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGt                                                                              <  1:4700436/101‑1 (MQ=255)
                    gATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGtt                                                                             <  1:4700283/101‑1 (MQ=255)
                    gATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGtt                                                                             <  2:4700493/101‑1 (MQ=255)
                    gATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGtt                                                                             <  1:4700653/101‑1 (MQ=255)
                     aTGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTa                                                                            <  1:4700419/101‑1 (MQ=255)
                      tGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAg                                                                           >  1:4700765/1‑101 (MQ=255)
                        aaCCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCt                                                                         <  1:4700393/101‑1 (MQ=255)
                           cGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCtttt                                                                      <  2:4700478/101‑1 (MQ=255)
                            gTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTa                                                                     <  2:4700307/101‑1 (MQ=255)
                              cGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTaaa                                                                   <  2:4700408/101‑1 (MQ=255)
                               ggCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAAt                                                                  <  1:4700424/101‑1 (MQ=255)
                               ggCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAAt                                                                  <  1:4700327/101‑1 (MQ=255)
                                 cGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCt                                                                >  2:4700771/1‑101 (MQ=255)
                                  gTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCtt                                                               >  1:4700772/1‑101 (MQ=255)
                                  gTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCtt                                                               <  2:4700519/101‑1 (MQ=255)
                                   tGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCttt                                                              >  1:4700775/1‑101 (MQ=255)
                                    gCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTc                                                             >  2:4700777/1‑101 (MQ=255)
                                      cACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCttt                                                              <  1:4700397/98‑1 (MQ=255)
                                      cACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCAc                                                           >  1:4700778/1‑101 (MQ=255)
                                      cACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCAc                                                           <  2:4700222/101‑1 (MQ=255)
                                        cTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCAcca                                                         >  1:4700783/1‑101 (MQ=255)
                                        cTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCAcca                                                         <  1:4700458/101‑1 (MQ=255)
                                        cTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCAcca                                                         <  2:4700321/101‑1 (MQ=255)
                                        cTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCAcca                                                         <  1:4700312/101‑1 (MQ=255)
                                         tGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCAccag                                                        <  2:4700279/101‑1 (MQ=255)
                                         tGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCAccag                                                        >  2:4700784/1‑101 (MQ=255)
                                          ggAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCAccagc                                                       >  2:4700786/1‑101 (MQ=255)
                                           gAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCAccagcg                                                      <  1:4700296/101‑1 (MQ=255)
                                            aaGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCAccagcgc                                                     >  2:4700791/1‑101 (MQ=255)
                                            aaGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTATTTGCACCGTTAGCTTTTAAATCTTTCAccagcgc                                                     >  1:4700788/1‑101 (MQ=255)
                                              gACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCGTCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCAccagcgcca                                                   <  2:4700341/101‑1 (MQ=255)
                                               acacCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCAccagcgccag                                                  >  2:4700794/1‑101 (MQ=255)
                                                  ccAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCgg                                               <  1:4700402/101‑1 (MQ=255)
                                                    aaaaTCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCt                                             >  2:4700796/1‑101 (MQ=255)
                                                    aaaaTCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCt                                             >  1:4700798/1‑101 (MQ=255)
                                                        tcaaatcCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATc                                         <  2:4700268/101‑1 (MQ=255)
                                                         caaatcCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCg                                        >  1:4700800/1‑101 (MQ=255)
                                                          aaatcCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGc                                       >  1:4700804/1‑101 (MQ=255)
                                                          aaatcCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGc                                       >  2:4700803/1‑101 (MQ=255)
                                                           aatcCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCt                                      >  1:4700809/1‑101 (MQ=255)
                                                             tcCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGc                                    >  2:4700811/1‑101 (MQ=255)
                                                             tcCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGc                                    >  1:4700812/1‑101 (MQ=255)
                                                              cccTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGcc                                   >  2:4700814/1‑101 (MQ=255)
                                                              cccTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGcc                                   >  1:4700816/1‑101 (MQ=255)
                                                               ccTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCg                                  <  1:4700194/101‑1 (MQ=255)
                                                               ccTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCg                                  <  1:4700473/101‑1 (MQ=255)
                                                                cTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCgg                                 >  1:4700821/1‑101 (MQ=255)
                                                                 ttAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGt                                <  1:4700315/101‑1 (MQ=255)
                                                                  tAAGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTa                               >  2:4700822/1‑101 (MQ=255)
                                                                    aGATCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACg                             >  1:4700824/1‑101 (MQ=255)
                                                                      aTCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCt                           >  1:4700827/1‑101 (MQ=255)
                                                                      aTCGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCt                           <  2:4700510/101‑1 (MQ=255)
                                                                        cGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCa                         >  2:4700829/1‑101 (MQ=255)
                                                                        cGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCa                         <  1:4700355/101‑1 (MQ=255)
                                                                        cGCCTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCa                         >  2:4700831/1‑101 (MQ=255)
                                                                          ccTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAg                       <  2:4700335/101‑1 (MQ=255)
                                                                           cTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCgg                                               <  2:4700538/76‑1 (MQ=255)
                                                                           cTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAgg                      >  1:4700833/1‑101 (MQ=255)
                                                                           cTTTTTCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAgg                      >  1:4700836/1‑101 (MQ=255)
                                                                            tttttCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAggg                     <  2:4700732/101‑1 (MQ=255)
                                                                             ttttCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGc                    >  1:4700839/1‑101 (MQ=255)
                                                                              tttCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGCa                   >  1:4700842/1‑101 (MQ=255)
                                                                               ttCATCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGCAg                  >  2:4700844/1‑101 (MQ=255)
                                                                                  aTCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGCAGTCg               <  2:4700460/101‑1 (MQ=255)
                                                                                  aTCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGCAGTCg               >  1:4700846/1‑101 (MQ=255)
                                                                                   tCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGCAGTCg               <  1:4700362/100‑1 (MQ=255)
                                                                                   tCCCAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGCAGTCGc              >  2:4700849/1‑101 (MQ=255)
                                                                                    cccAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGCAGTCGcc             >  1:4700850/1‑101 (MQ=255)
                                                                                     ccAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCCCCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGCAGTCGCCa            <  1:4700290/101‑1 (MQ=255)
                                                                                      cAGTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGCAGTCGCCag           >  2:4700853/1‑101 (MQ=255)
                                                                                        gTTCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGCAGTCGCCagag         <  2:4700399/101‑1 (MQ=255)
                                                                                         ttCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGCAGTCGCCAgaga        >  2:4700857/1‑101 (MQ=255)
                                                                                         ttCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGCAGTCGCCAgaga        >  1:4700856/1‑101 (MQ=255)
                                                                                         ttCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGCAGTCGCCAgaga        <  1:4700451/101‑1 (MQ=255)
                                                                                          tCAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGCAGTCGCCAGAGAt       >  2:4700860/1‑101 (MQ=255)
                                                                                           cAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGCAGTCGCCAGAGAtt      <  2:4700489/101‑1 (MQ=255)
                                                                                           cAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGCAGTCGCCAGAGAtt      >  1:4700861/1‑101 (MQ=255)
                                                                                           cAGCGGCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGCAGTCGCCAGAGAtt      <  2:4700354/101‑1 (MQ=255)
                                                                                               ggCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGCAGTCGCCAGAGATTGCag  <  2:4700610/101‑2 (MQ=255)
                                                                                               ggCTCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGCAGTCGCCAGAGATTGCAc  <  1:4700527/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
ATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGCAGTCGCCAGAGATTGCAC  >  NC_000913/3596447‑3596642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑