BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,794,218	T→C	C78R (TGC→CGC)	rfaD →	ADP‑L‑glycero‑D‑mannoheptose 6‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,794,218	0	T	C	100.0%	847.8 / NA	207	C78R (TGC→CGC)	rfaD	ADP‑L‑glycero‑D‑mannoheptose 6‑epimerase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (80/127); total (80/127)

Only 100 of 217 total aligned reads displayed.

TGAATATCGCAGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGTGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCC  >  NC_000913/3794120‑3794315

        gCAGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTccac                                                                                         <  2:4982744/101‑1 (MQ=255)

         cAGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTccacc                                                                                        <  1:4982719/101‑1 (MQ=255)

            aCTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCaccacc                                                                                     <  1:4982733/101‑1 (MQ=255)

            aCTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCaccacc                                                                                     <  1:4982423/101‑1 (MQ=255)

              tataTGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGa                                                                                   <  1:4982586/101‑1 (MQ=255)

               atatGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCg                                                                                    <  2:4982583/99‑1 (MQ=255)

                tatGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGt                                                                                 <  1:4982659/101‑1 (MQ=255)

                tatGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGt                                                                                 <  2:4982607/101‑1 (MQ=255)

                 atGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTg                                                                                <  2:4982501/101‑1 (MQ=255)

                 atGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTg                                                                                <  1:4982320/101‑1 (MQ=255)

                     aTAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTggg                                                                              >  1:4983040/1‑99 (MQ=255)

                     aTAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGAc                                                                            <  1:4982508/101‑1 (MQ=255)

                      tAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACAACCGAGTGGGACg                                                                           <  1:4982724/101‑1 (MQ=255)

                       aaGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACgg                                                                          <  1:4982538/101‑1 (MQ=255)

                       aaGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACgg                                                                          <  1:4982814/101‑1 (MQ=255)

                        aGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGc                                                                         <  1:4982632/101‑1 (MQ=255)

                         ggAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCa                                                                        <  1:4982619/101‑1 (MQ=255)

                             gACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGta                                                                    <  1:4982696/101‑1 (MQ=255)

                              aCTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGtat                                                                   <  1:4982746/101‑1 (MQ=255)

                              aCTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGtat                                                                   <  1:4982689/101‑1 (MQ=255)

                              aCTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGtat                                                                   <  2:4982473/101‑1 (MQ=255)

                              aCTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGtat                                                                   >  1:4983049/1‑101 (MQ=255)

                               cTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGtata                                                                  <  1:4982550/101‑1 (MQ=255)

                                ttCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTatat                                                                 <  1:4982957/101‑1 (MQ=255)

                                ttCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTatat                                                                 <  1:4982735/101‑1 (MQ=255)

                                 tCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATatg                                                                <  1:4982223/101‑1 (MQ=255)

                                     gATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATgg                                                            >  1:4983051/1‑101 (MQ=255)

                                     gATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATgg                                                            <  2:4982709/101‑1 (MQ=255)

                                      aTCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGa                                                           <  2:4982568/101‑1 (MQ=255)

                                         cAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATa                                                         <  1:4982849/100‑1 (MQ=255)

                                            aTTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATaacaa                                                     >  1:4983054/1‑101 (MQ=255)

                                            aTTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATaacaa                                                     <  2:4982404/101‑1 (MQ=255)

                                             ttATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATaacaac                                                    >  1:4983057/1‑101 (MQ=255)

                                             ttATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATaacaac                                                    <  1:4982474/101‑1 (MQ=255)

                                              tATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACt                                                   <  2:4982683/101‑1 (MQ=255)

                                              tATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACt                                                   <  2:4982791/101‑1 (MQ=255)

                                               aTGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGgcgcgcgc                                                                                                 >  2:4983058/1‑54 (MQ=255)

                                               aTGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTa                                                  <  2:4982785/101‑1 (MQ=255)

                                                 ggctggcGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATc                                                <  2:4982741/101‑1 (MQ=255)

                                                 ggctggcGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATc                                                >  1:4983060/1‑101 (MQ=255)

                                                 ggctggcGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATc                                                <  1:4982286/101‑1 (MQ=255)

                                                 ggctggcGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATc                                                <  1:4982762/101‑1 (MQ=255)

                                                   ctggcGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCaa                                              <  1:4982612/101‑1 (MQ=255)

                                                   ctggcGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCaa                                              <  1:4982698/101‑1 (MQ=255)

                                                   ctggcGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCaa                                              >  2:4983062/1‑101 (MQ=255)

                                                    tggcGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAAt                                             >  2:4983064/1‑101 (MQ=255)

                                                     ggcGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGt                                                                                 <  2:4982713/64‑1 (MQ=255)

                                                     ggcGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATa                                            >  2:4983066/1‑101 (MQ=255)

                                                     ggcGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATa                                            <  1:4982772/101‑1 (MQ=255)

                                                      gcGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATAc                                           >  1:4983068/1‑101 (MQ=255)

                                                        gAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTc                                         <  2:4982811/101‑1 (MQ=255)

                                                        gAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTc                                         >  1:4983071/1‑101 (MQ=255)

                                                        gAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTc                                         >  1:4983074/1‑101 (MQ=255)

                                                         aaGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTcc                                        <  1:4982795/101‑1 (MQ=255)

                                                         aaGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTcc                                        >  2:4983079/1‑101 (MQ=255)

                                                         aaGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTcc                                        >  1:4983076/1‑101 (MQ=255)

                                                          agagTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGTCAAGTATATGATGGATAACAACTATCAATACTCCa                                       >  1:4983081/1‑101 (MQ=255)

                                                           gagTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCaa                                      >  1:4983083/1‑101 (MQ=255)

                                                           gagTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCaa                                      <  1:4982821/101‑1 (MQ=255)

                                                            agTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATGTGATGGATAACAACTATCAATACTCCAAg                                     <  1:4982562/101‑1 (MQ=255)

                                                            agTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAg                                     <  2:4982665/101‑1 (MQ=255)

                                                            agTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAg                                     >  2:4983087/1‑101 (MQ=255)

                                                            agTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAg                                     >  1:4983085/1‑101 (MQ=255)

                                                            agTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAg                                     >  1:4983088/1‑101 (MQ=255)

                                                             gTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAgg                                    <  2:4982781/101‑1 (MQ=255)

                                                              ttCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAg                                                                                  >  2:4983090/1‑54 (MQ=255)

                                                              ttCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGa                                   <  1:4982825/101‑1 (MQ=255)

                                                               tCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAg                                                                                  >  1:4983093/1‑53 (MQ=255)

                                                               tCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAg                                  <  2:4982695/101‑1 (MQ=255)

                                                                cGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACg                                                                           >  1:4983096/1‑59 (MQ=255)

                                                                 ggCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAgct                                >  2:4983099/1‑101 (MQ=255)

                                                                 ggCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAgct                                <  1:4982664/101‑1 (MQ=255)

                                                                 ggCGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAgct                                >  1:4983098/1‑101 (MQ=255)

                                                                   cGATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAgctgc                              >  1:4983101/1‑101 (MQ=255)

                                                                    gATGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAgctgct                             >  2:4983104/1‑101 (MQ=255)

                                                                     aTGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGctgctg                            >  2:4983106/1‑101 (MQ=255)

                                                                     aTGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGctgctg                            <  1:4982991/101‑1 (MQ=255)

                                                                     aTGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGctgctg                            >  1:4983108/1‑101 (MQ=255)

                                                                     aTGTCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGctgctg                            <  2:4982703/101‑1 (MQ=255)

                                                                        tCGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATTCTCCAAGGAGCTGCTGCac                         >  2:4983110/1‑101 (MQ=255)

                                                                         cGAAGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCac                         <  1:4982780/100‑1 (MQ=255)

                                                                            aGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAgc                                 <  1:4982626/89‑1 (MQ=255)

                                                                            aGCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCactact                     <  2:4982629/101‑1 (MQ=255)

                                                                             gCGATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTg                    <  2:4982691/101‑1 (MQ=255)

                                                                               gATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGcc                  >  2:4983113/1‑101 (MQ=255)

                                                                               gATTTTCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGcc                  >  1:4983114/1‑101 (MQ=255)

                                                                                 ttttCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTg                >  2:4983117/1‑101 (MQ=255)

                                                                                 ttttCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTg                >  1:4983118/1‑101 (MQ=255)

                                                                                  tttCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTgg               <  1:4982681/101‑1 (MQ=255)

                                                                                  tttCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTgg               <  2:4982840/101‑1 (MQ=255)

                                                                                    tCCACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAg             <  2:4982871/101‑1 (MQ=255)

                                                                                     ccACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGc            >  1:4983122/1‑101 (MQ=255)

                                                                                      cACGAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCg           >  2:4983123/1‑101 (MQ=255)

                                                                                         gAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTc                                         <  1:4982647/68‑1 (MQ=255)

                                                                                         gAAGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGa        >  1:4983126/1‑101 (MQ=255)

                                                                                          aaGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGaa       <  1:4982534/101‑1 (MQ=255)

                                                                                          aaGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGaa       >  2:4983128/1‑101 (MQ=255)

                                                                                           aGGCGCGCGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGaaa      <  1:4982751/101‑1 (MQ=255)

                                                                                               gcgcgcTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATccc  <  2:4982649/101‑1 (MQ=255)

                                                                                               gcgcgcTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATccc  >  1:4983131/1‑101 (MQ=255)

TGAATATCGCAGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGTGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCC  >  NC_000913/3794120‑3794315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`