Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,342,046 T→C R42R (CGT→CGC)  pyrF → orotidine‑5'‑phosphate decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,342,0460TC100.0% 952.9 / NA 233R42R (CGT→CGC) pyrForotidine‑5'‑phosphate decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (98/135);  total (98/135)

Only 100 of 239 total aligned reads displayed.

CGCGCTGTTACGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCC  >  NC_000913/1341948‑1342143
                                                                                                  |                                                                                                 
cgcgCTGTTACGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCt                                                                                                 <  2:1749245/101‑1 (MQ=255)
 gcgcTGTTACGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTg                                                                                                >  1:1749525/1‑101 (MQ=255)
  cgcTGTTACGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGa                                                                                               >  1:1749527/1‑101 (MQ=255)
    cTGTTACGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAg                                                                                             >  2:1749530/1‑101 (MQ=255)
    cTGTTACGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAg                                                                                             <  2:1749171/101‑1 (MQ=255)
       ttACGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTc                                                                                          >  1:1749535/1‑101 (MQ=255)
       ttACGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTc                                                                                          <  1:1749051/101‑1 (MQ=255)
       ttACGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTc                                                                                          >  2:1749534/1‑101 (MQ=255)
        tACGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCg                                                                                         <  2:1749338/101‑1 (MQ=255)
        tACGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCg                                                                                         <  1:1749129/101‑1 (MQ=255)
         aCGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCgg                                                                                        <  1:1749197/101‑1 (MQ=255)
          cGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGc                                                                                       >  2:1749539/1‑101 (MQ=255)
           gAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCa                                                                                      >  2:1749541/1‑101 (MQ=255)
           gAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCa                                                                                      >  1:1749542/1‑101 (MQ=255)
           gAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCa                                                                                      <  1:1749103/101‑1 (MQ=255)
           gAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCa                                                                                      <  1:1749070/101‑1 (MQ=255)
           gAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCa                                                                                      <  1:1749026/101‑1 (MQ=255)
           gAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAACGTCGGCa                                                                                      <  1:1749170/101‑1 (MQ=255)
             aTTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCaaa                                                                                    <  1:1749431/101‑1 (MQ=255)
             aTTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCaaa                                                                                    <  1:1749212/101‑1 (MQ=255)
             aTTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCaaa                                                                                    <  1:1749234/101‑1 (MQ=255)
               tctcCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAaga                                                                                  >  2:1749544/1‑101 (MQ=255)
                 tcCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAgaga                                                                                <  1:1749301/101‑1 (MQ=255)
                   cTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATg                                                                              >  1:1749549/1‑101 (MQ=255)
                   cTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATg                                                                              <  2:1748996/101‑1 (MQ=255)
                   cTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATg                                                                              <  1:1748843/101‑1 (MQ=255)
                      tgGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGttt                                                                           >  2:1749551/1‑101 (MQ=255)
                       ggTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTa                                                                          >  2:1749553/1‑101 (MQ=255)
                          tgttgCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACAt                                                                       >  2:1749556/1‑101 (MQ=255)
                          tgttgCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACAt                                                                       <  1:1749248/101‑1 (MQ=255)
                            ttgCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGa                                                                                               <  1:1749204/75‑1 (MQ=255)
                            ttgCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTg                                                                     <  2:1749018/101‑1 (MQ=255)
                             tgCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGt                                                                    >  2:1749559/1‑101 (MQ=255)
                              gCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGtt                                                                   >  1:1749560/1‑101 (MQ=255)
                               cccTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGttt                                                                  <  2:1749474/101‑1 (MQ=255)
                                ccTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTg                                                                 >  2:1749564/1‑101 (MQ=255)
                                   tGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGc                                                              >  1:1749566/1‑101 (MQ=255)
                                   tGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGc                                                              <  1:1749351/101‑1 (MQ=255)
                                    gATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTc                                                                                          >  2:1749568/1‑72 (MQ=255)
                                    gATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGcc                                                             <  2:1749218/101‑1 (MQ=255)
                                     aTTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCca                                                            <  1:1749147/101‑1 (MQ=255)
                                     aTTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCca                                                            >  1:1749570/1‑101 (MQ=255)
                                      ttATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCcac                                                           <  1:1749285/101‑1 (MQ=255)
                                       tATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCcaca                                                          <  1:1748969/101‑1 (MQ=255)
                                       tATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCcaca                                                          >  2:1749572/1‑101 (MQ=255)
                                       tATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCcaca                                                          <  2:1749123/101‑1 (MQ=255)
                                        aTCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAg                                                         <  1:1749095/101‑1 (MQ=255)
                                         tCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGt                                                        >  2:1749574/1‑101 (MQ=255)
                                         tCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGt                                                        <  1:1749134/101‑1 (MQ=255)
                                            tAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTtg                                                     <  1:1749257/101‑1 (MQ=255)
                                            tAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTtg                                                     <  2:1748924/101‑1 (MQ=255)
                                            tAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGAGCCACAGTTtg                                                     <  1:1749111/101‑1 (MQ=255)
                                             aaTCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTtgt                                                    >  2:1749578/1‑101 (MQ=255)
                                                cGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTgcg                                                 >  2:1749580/1‑101 (MQ=255)
                                                cGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTgcg                                                 <  2:1749096/101‑1 (MQ=255)
                                                 gTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTgcgc                                                <  2:1749408/101‑1 (MQ=255)
                                                  tgatgaCGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAagag                                                                                 >  1:1749584/1‑67 (MQ=255)
                                                   gatgaCGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCa                                                                                      >  2:1749586/1‑61 (MQ=255)
                                                      gaCGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACt                                           >  2:1749589/1‑101 (MQ=255)
                                                      gaCGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACt                                           >  1:1749590/1‑101 (MQ=255)
                                                       aCGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACtt                                          <  2:1749258/101‑1 (MQ=255)
                                                          cgcTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCaa                                       <  1:1749055/101‑1 (MQ=255)
                                                          cgcTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGCCg                                                                                         >  1:1749594/1‑51 (MQ=255)
                                                           gcTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAAc                                      >  2:1749596/1‑101 (MQ=255)
                                                           gcTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAAc                                      <  1:1749185/101‑1 (MQ=255)
                                                            cTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACa                                     <  1:1749294/101‑1 (MQ=255)
                                                               gCCTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCg                                  <  1:1749410/101‑1 (MQ=255)
                                                                 cTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCg                                  >  2:1749604/1‑99 (MQ=255)
                                                                 cTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTg                                >  2:1749602/1‑101 (MQ=255)
                                                                 cTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTg                                >  1:1749601/1‑101 (MQ=255)
                                                                 cTTTGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTg                                <  2:1749194/101‑1 (MQ=255)
                                                                  tttGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTgg                               <  1:1749023/101‑1 (MQ=255)
                                                                  tttGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTgg                               <  1:1749307/101‑1 (MQ=255)
                                                                   ttGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTgg                               <  2:1749222/100‑1 (MQ=255)
                                                                    tGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGtt                             <  2:1749151/101‑1 (MQ=255)
                                                                    tGTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGtt                             >  1:1749608/1‑101 (MQ=255)
                                                                     gTCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAagag                                                                                 >  2:1749609/1‑48 (MQ=255)
                                                                      tCGGCAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCGAag                                                                                   <  2:1749205/45‑1 (MQ=38)
                                                                      tCGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGtttt                           <  1:1749277/101‑1 (MQ=255)
                                                                       cGACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTg                          <  1:1749127/101‑1 (MQ=255)
                                                                        gACAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGa                         >  2:1749612/1‑101 (MQ=255)
                                                                         aCAAGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGat                        <  1:1749188/101‑1 (MQ=255)
                                                                           aaGATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGatat                      <  1:1749452/101‑1 (MQ=255)
                                                                             gATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATAtct                    <  2:1749118/101‑1 (MQ=255)
                                                                             gATCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATAtct                    >  2:1749615/1‑101 (MQ=255)
                                                                              aTCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATAtctt                   <  2:1749270/101‑1 (MQ=255)
                                                                              aTCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATAtctt                   <  2:1749192/101‑1 (MQ=255)
                                                                              aTCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATAtctt                   <  2:1749388/101‑1 (MQ=255)
                                                                               tCGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATAtcttt                  <  2:1749179/101‑1 (MQ=255)
                                                                                cGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATAtctttc                 <  2:1749052/101‑1 (MQ=255)
                                                                                cGACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATAtctttc                 <  2:1749250/101‑1 (MQ=255)
                                                                                 gACCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATAtctttct                <  2:1749260/101‑1 (MQ=255)
                                                                                  aCCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATAtctttctt               >  2:1749618/1‑101 (MQ=255)
                                                                                  aCCCACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATAtctttctt               <  2:1749328/101‑1 (MQ=255)
                                                                                     cACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGAc            >  2:1749620/1‑101 (MQ=255)
                                                                                     cACGCGATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGAc            >  1:1749621/1‑101 (MQ=255)
                                                                                        gcgATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTg         >  1:1749624/1‑101 (MQ=255)
                                                                                          gATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCg                                  <  1:1748950/74‑1 (MQ=255)
                                                                                          gATTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGaa       >  1:1749627/1‑101 (MQ=255)
                                                                                           aTTGTCGCCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGaaa      <  1:1749321/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CGCGCTGTTACGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCC  >  NC_000913/1341948‑1342143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑