Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 205,987 A→G T288A (ACC→GCC)  dnaE → DNA polymerase III subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913205,9870AG99.6% 1031.3 / NA 257T288A (ACC→GCC) dnaEDNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (125/131);  total (126/131)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 257 total aligned reads displayed.

AAGCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAGCGCCGCCCGG  >  NC_000913/205889‑206083
                                                                                                  |                                                                                                
aaGCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGcc                                                                                                >  1:273668/1‑101 (MQ=255)
 aGCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCg                                                                                               <  2:272866/101‑1 (MQ=255)
  gCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGa                                                                                              <  2:273383/101‑1 (MQ=255)
  gCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGa                                                                                              >  1:273674/1‑101 (MQ=255)
  gCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGa                                                                                              >  2:273672/1‑101 (MQ=255)
    ccTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAg                                                                                            <  1:273382/101‑1 (MQ=255)
      ttGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGAt                                                                                          >  1:273679/1‑101 (MQ=255)
       tGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGAtt                                                                                         <  2:273308/101‑1 (MQ=255)
        gCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTa                                                                                        >  1:273681/1‑101 (MQ=255)
         ccAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTAt                                                                                       >  1:273684/1‑101 (MQ=255)
          cAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTTCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATc                                                                                      <  2:273050/101‑1 (MQ=255)
          cAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATc                                                                                      >  1:273687/1‑101 (MQ=255)
            acacCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTg                                                                                    >  2:273690/1‑101 (MQ=255)
              acCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGt                                                                                  >  1:273692/1‑101 (MQ=255)
               ccGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTc                                                                                 <  1:273071/101‑1 (MQ=255)
               ccGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTc                                                                                 >  1:273694/1‑101 (MQ=255)
                 gTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCaa                                                                               <  2:273392/101‑1 (MQ=255)
                  ttGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAg                                                                              >  2:273698/1‑101 (MQ=255)
                   tGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGc                                                                             <  1:273396/101‑1 (MQ=255)
                      gaTCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTg                                                                          <  2:273447/101‑1 (MQ=255)
                           ccAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCaaaa                                                                     <  1:273349/101‑1 (MQ=255)
                           ccAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCaaaa                                                                     >  1:273703/1‑101 (MQ=255)
                            cAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAag                                                                    >  2:273705/1‑101 (MQ=255)
                            cAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAag                                                                    <  1:273307/101‑1 (MQ=255)
                              aaCGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAg                                                                              <  1:273170/89‑1 (MQ=255)
                              aaCGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAagag                                                                  >  1:273708/1‑101 (MQ=255)
                              aaCGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAagag                                                                  <  1:273100/101‑1 (MQ=255)
                               aCGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAgg                                                                 <  1:273359/101‑1 (MQ=255)
                                cGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAggg                                                                <  1:273301/101‑1 (MQ=255)
                                  cTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGcc                                                              >  2:273710/1‑101 (MQ=255)
                                  cTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGcc                                                              <  1:273250/101‑1 (MQ=255)
                                   tGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCt                                                             <  2:273528/101‑1 (MQ=255)
                                   tGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCt                                                             >  1:273711/1‑101 (MQ=255)
                                    gtaacgtaacCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTg                                                            <  2:273345/101‑1 (MQ=255)
                                     taacgtaacCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTgg                                                           <  2:273405/101‑1 (MQ=255)
                                      aacgtaacCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGa                                                          >  2:273715/1‑101 (MQ=255)
                                       acgtaacCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGaa                                                         >  1:273717/1‑101 (MQ=255)
                                         gtaacCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAaga                                                       >  2:273721/1‑101 (MQ=255)
                                         gtaacCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAaga                                                       >  1:273720/1‑101 (MQ=255)
                                           aacCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGc                                                     <  2:273478/101‑1 (MQ=255)
                                           aacCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGc                                                     <  1:273256/101‑1 (MQ=255)
                                           aacCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGc                                                     <  1:273645/101‑1 (MQ=255)
                                           aacCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGc                                                     <  2:273182/101‑1 (MQ=255)
                                           aacCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGc                                                     <  2:273242/101‑1 (MQ=255)
                                             ccGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGt                                                   <  2:273264/101‑1 (MQ=255)
                                              cGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTg                                                            <  1:273279/91‑1 (MQ=255)
                                              cGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTc                                                  >  2:273726/1‑101 (MQ=255)
                                               gTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAg                                                                              <  2:273357/72‑1 (MQ=255)
                                               gTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCt                                                 <  2:273374/101‑1 (MQ=255)
                                               gTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCt                                                 <  2:273465/101‑1 (MQ=255)
                                                 gCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTgg                                               <  1:273492/101‑1 (MQ=255)
                                                 gCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTgg                                               <  1:273438/101‑1 (MQ=255)
                                                   gTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGcc                                             <  2:273158/101‑1 (MQ=255)
                                                   gTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGcc                                             >  1:273731/1‑101 (MQ=255)
                                                   gTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGcc                                             >  1:273729/1‑101 (MQ=255)
                                                    tCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCt                                            >  2:273733/1‑101 (MQ=255)
                                                    tCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCt                                            <  1:273460/101‑1 (MQ=255)
                                                     cTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCtt                                           <  1:273325/101‑1 (MQ=255)
                                                     cTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCtt                                           >  1:273735/1‑101 (MQ=255)
                                                      ttGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCg                                                    <  1:273343/91‑1 (MQ=255)
                                                      ttGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCttt                                          >  2:273737/1‑101 (MQ=255)
                                                      ttGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCttt                                          <  1:273115/101‑1 (MQ=255)
                                                       tGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCtttt                                         <  1:273489/101‑1 (MQ=255)
                                                        ggTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCttttt                                        <  1:273318/101‑1 (MQ=255)
                                                           gAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTAtt                                     >  2:273742/1‑101 (MQ=255)
                                                               aCTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCt                                 <  1:273525/101‑1 (MQ=255)
                                                               aCTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCt                                 >  1:273744/1‑101 (MQ=255)
                                                                cTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCtg                                >  2:273745/1‑101 (MQ=255)
                                                                 ttCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCtga                               <  1:273408/101‑1 (MQ=255)
                                                                 ttCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCtga                               <  2:273679/101‑1 (MQ=255)
                                                                  tCCTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCtgat                              >  1:273747/1‑101 (MQ=255)
                                                                    cTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCtgatga                            >  2:273754/1‑101 (MQ=255)
                                                                    cTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCtgatga                            <  1:273434/101‑1 (MQ=255)
                                                                    cTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCtgatga                            >  2:273751/1‑101 (MQ=255)
                                                                    cTGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCtgatga                            <  2:273516/101‑1 (MQ=255)
                                                                     tGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAg                           >  2:273760/1‑101 (MQ=255)
                                                                     tGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAg                           <  1:273195/101‑1 (MQ=255)
                                                                     tGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAg                           >  1:273757/1‑101 (MQ=255)
                                                                     tGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAg                           <  2:273634/101‑1 (MQ=255)
                                                                     tGCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAg                           >  1:273759/1‑101 (MQ=255)
                                                                      gCCGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAgg                          <  2:273422/101‑1 (MQ=255)
                                                                       ccGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGga                         >  1:273765/1‑101 (MQ=255)
                                                                        cGCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGgaa                        >  2:273766/1‑101 (MQ=255)
                                                                         gCAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGgaag                       >  2:273769/1‑101 (MQ=255)
                                                                          cAGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGgaaga                      <  1:273541/101‑1 (MQ=255)
                                                                           aGTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGgaagaa                     >  1:273772/1‑101 (MQ=255)
                                                                            gTTCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAAc                    >  2:273773/1‑101 (MQ=255)
                                                                             ttCCCGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACg                   >  2:273776/1‑101 (MQ=255)
                                                                                 cGACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCtt               >  1:273780/1‑101 (MQ=255)
                                                                                  gACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTa              >  1:273784/1‑101 (MQ=255)
                                                                                  gACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTa              <  1:273425/101‑1 (MQ=255)
                                                                                  gACCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTa              >  2:273783/1‑101 (MQ=255)
                                                                                   aCCGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTaa             <  2:273484/101‑1 (MQ=255)
                                                                                    ccGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAag                                                                    <  2:273284/45‑1 (MQ=255)
                                                                                    ccGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAg            <  2:273458/101‑1 (MQ=255)
                                                                                    ccGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAg            >  1:273787/1‑101 (MQ=255)
                                                                                     cGGGGACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAgc           >  2:273790/1‑101 (MQ=255)
                                                                                      ggggACATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAgcg          >  1:273791/1‑101 (MQ=255)
                                                                                          aCATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAGcgccgc      >  2:273794/1‑101 (MQ=255)
                                                                                           cATGAGCGCCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAGCgccgcc     <  1:273215/101‑1 (MQ=255)
                                                                                                  |                                                                                                
AAGCCCTTGCCAACACCGTTGAGATCGCCAAACGCTGTAACGTAACCGTGCGTCTTGGTGAATACTTCCTGCCGCAGTTCCCGACCGGGGACATGAGCACCGAAGATTATCTGGTCAAGCGTGCAAAAGAGGGCCTGGAAGAGCGTCTGGCCTTTTTATTCCCTGATGAGGAAGAACGTCTTAAGCGCCGCCCGG  >  NC_000913/205889‑206083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑