Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,620,807 G→A R111* (CGA→TGA)  eamA ← cysteine/O‑acetylserine exporter EamA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,620,8070GA99.6% 934.2 / NA 245R111* (CGA→TGA) eamAcysteine/O‑acetylserine exporter EamA
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (128/116);  total (128/117)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

GAGCATCGCCACATGCTGACCGTTCAGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCGCTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCA  >  NC_000913/1620709‑1620904
                                                                                                  |                                                                                                 
 aGCATCGCCACATGCTGACCGTTCAGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTc                                                                                                <  1:2117457/101‑1 (MQ=255)
    aTCGCCACATGCTGACCGTTCAGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTcccc                                                                                             <  2:2117385/101‑1 (MQ=255)
    aTCGCCACATGCTGACCGTTCAGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTcccc                                                                                             <  1:2117462/101‑1 (MQ=255)
     tCGCCACATGCTGACCGTTCAGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCg                                                                                            <  2:2117221/101‑1 (MQ=255)
     tCGCCACATGCTGACCGTTCAGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCg                                                                                            <  1:2117363/101‑1 (MQ=255)
       gCCACATGCTGACCGTTCAGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGaa                                                                                          >  1:2117774/1‑101 (MQ=255)
        ccACATGCTGACCGTTCAGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGaaa                                                                                         >  2:2117776/1‑101 (MQ=255)
        ccACATGCTGACCGTTCAGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGaaa                                                                                         <  2:2117441/101‑1 (MQ=255)
         cacaTGCTGACCGTTCAGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAg                                                                                        >  1:2117778/1‑101 (MQ=255)
          acaTGCTGACCGTTCAGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGt                                                                                       <  1:2117256/101‑1 (MQ=255)
          acaTGCTGACCGTTCAGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGt                                                                                       >  2:2117779/1‑101 (MQ=255)
           caTGCTGACCGTTCAGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTa                                                                                      <  1:2117502/101‑1 (MQ=255)
             tGCTGACCGTTCAGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTaaa                                                                                    <  1:2117580/101‑1 (MQ=255)
               cTGACCGTTCAGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAAcg                                                                                  >  1:2117785/1‑101 (MQ=255)
               cTGACCGTTCAGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAAcg                                                                                  >  2:2117784/1‑101 (MQ=255)
                tGACCGTTCAGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAAcgc                                                                                 <  1:2117569/101‑1 (MQ=255)
                 gACCGTTCAGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAAcgcg                                                                                <  1:2117393/101‑1 (MQ=255)
                  aCCGTTCAGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACgcgc                                                                               >  2:2117787/1‑101 (MQ=255)
                     gTTCAGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCaa                                                                            >  1:2117791/1‑101 (MQ=255)
                      ttCAGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAg                                                                           <  2:2117415/101‑1 (MQ=255)
                        cAGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCa                                                                         <  2:2117530/101‑1 (MQ=255)
                         aGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCAt                                                                        >  2:2117794/1‑101 (MQ=255)
                         aGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCAt                                                                        >  1:2117795/1‑101 (MQ=255)
                          gACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATg                                                                       <  1:2117073/101‑1 (MQ=255)
                            cTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGAt                                                                     >  1:2117800/1‑101 (MQ=255)
                            cTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGAt                                                                     >  2:2117799/1‑101 (MQ=255)
                               tCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGt                                                                  >  1:2117803/1‑101 (MQ=255)
                                cTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTa                                                                 >  2:2117805/1‑101 (MQ=255)
                                   cGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTaaaa                                                              >  2:2117807/1‑101 (MQ=255)
                                    gATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTaaaaa                                                             >  2:2117809/1‑101 (MQ=255)
                                      ttAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAAc                                                           >  2:2117813/1‑101 (MQ=255)
                                         acacCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGAt                                                                     <  1:2117418/88‑1 (MQ=255)
                                         acacCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACgcc                                                        <  2:2117409/101‑1 (MQ=255)
                                           acCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACgcctg                                                      >  1:2117817/1‑101 (MQ=255)
                                           acCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACgcctg                                                      <  2:2117544/101‑1 (MQ=255)
                                           acCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACgcctg                                                      >  2:2117816/1‑101 (MQ=255)
                                             cAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACgcctctg                                                    <  1:2117524/101‑1 (MQ=255)
                                              aGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACgcctgtgc                                                   <  1:2117444/101‑1 (MQ=255)
                                                tACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACgcctgtgcct                                                 >  1:2117820/1‑101 (MQ=255)
                                                  cGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACgcctgtgcctgt                                               >  1:2117825/1‑101 (MQ=255)
                                                  cGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACgcctgtgcctgt                                               >  2:2117824/1‑101 (MQ=255)
                                                  cGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACgcctgtgcctgt                                               <  1:2117598/101‑1 (MQ=255)
                                                   gCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTa                                              <  2:2117394/101‑1 (MQ=255)
                                                     cAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAac                                            >  2:2117828/1‑101 (MQ=255)
                                                      aaaaaTCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACgcctgtgcct                                                 <  1:2117432/95‑1 (MQ=255)
                                                      aaaaaTCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAaca                                           <  1:2117357/101‑1 (MQ=255)
                                                      aaaaaTCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAaca                                           >  1:2117832/1‑101 (MQ=255)
                                                      aaaaaTCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAaca                                           >  2:2117833/1‑101 (MQ=255)
                                                         aaTCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCa                                        >  1:2117836/1‑101 (MQ=255)
                                                           tCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGc                                      <  2:2117663/101‑1 (MQ=255)
                                                            cGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCg                                     >  1:2117839/1‑101 (MQ=255)
                                                              cTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGaa                                   >  2:2117842/1‑101 (MQ=255)
                                                              cTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGaa                                   >  2:2117845/1‑101 (MQ=255)
                                                              cTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGaa                                   <  2:2117350/101‑1 (MQ=255)
                                                               tAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAg                                  >  2:2117849/1‑101 (MQ=255)
                                                               tAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAg                                  >  1:2117847/1‑101 (MQ=255)
                                                                aaGGCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGc                                 >  1:2117851/1‑101 (MQ=255)
                                                                   gCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAg                              <  1:2117648/101‑1 (MQ=255)
                                                                   gCGATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAg                              >  2:2117852/1‑101 (MQ=255)
                                                                     gctCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGaaa                                                                                         <  2:2117147/38‑1 (MQ=255)
                                                                     gATCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTc                            >  1:2117856/1‑101 (MQ=255)
                                                                      aTCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTcc                           <  1:2117407/101‑1 (MQ=255)
                                                                      aTCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTcc                           >  1:2117860/1‑101 (MQ=255)
                                                                       tCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCca                          <  2:2117467/101‑1 (MQ=255)
                                                                       tCCCCGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCca                          >  2:2117861/1‑101 (MQ=255)
                                                                        ccccGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACg                                                          <  2:2117308/68‑1 (MQ=255)
                                                                        ccccGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGcc                                <  1:2117741/94‑1 (MQ=255)
                                                                        ccccGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCcag                         <  1:2117320/101‑1 (MQ=255)
                                                                         cccGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCcagc                        >  1:2117863/1‑101 (MQ=255)
                                                                          ccGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGa                                    <  2:2117377/88‑1 (MQ=255)
                                                                          ccGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCcagca                       <  1:2117533/101‑1 (MQ=255)
                                                                          ccGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCcag                         <  1:2117273/99‑1 (MQ=255)
                                                                           cGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCcagcag                      >  2:2117866/1‑101 (MQ=255)
                                                                           cGCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCcagcag                      <  2:2117577/101‑1 (MQ=255)
                                                                            gCCAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAgg                     >  1:2117869/1‑101 (MQ=255)
                                                                              cAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCa                   <  2:2117628/101‑1 (MQ=255)
                                                                              cAATTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCa                   >  1:2117872/1‑101 (MQ=255)
                                                                               aaTTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCAt                  <  2:2117620/101‑1 (MQ=255)
                                                                               aaTTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCAt                  >  2:2117874/1‑101 (MQ=255)
                                                                               aaTTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCAt                  <  1:2117474/101‑1 (MQ=255)
                                                                                aTTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATa                 <  2:2117557/101‑1 (MQ=255)
                                                                                aTTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATa                 <  2:2117388/101‑1 (MQ=255)
                                                                                aTTGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATa                 >  2:2117876/1‑101 (MQ=255)
                                                                                 ttGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATAc                >  1:2117878/1‑101 (MQ=255)
                                                                                  tGTTTGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATAcc               <  1:2117403/101‑1 (MQ=255)
                                                                                    tttGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGa             >  1:2117880/1‑101 (MQ=255)
                                                                                      tGCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAg           >  2:2117883/1‑101 (MQ=255)
                                                                                       gCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGt          <  1:2117618/101‑1 (MQ=255)
                                                                                       gCCATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGt          <  1:2117413/101‑1 (MQ=255)
                                                                                        ccATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGt          <  2:2117364/100‑1 (MQ=255)
                                                                                         cATGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTa        >  1:2117887/1‑101 (MQ=255)
                                                                                          aTGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTaa       >  2:2117889/1‑101 (MQ=255)
                                                                                           tGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGaa            <  2:2117497/95‑1 (MQ=255)
                                                                                           tGCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAAt      >  1:2117890/1‑101 (MQ=255)
                                                                                            gCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATg     <  2:2117509/101‑1 (MQ=255)
                                                                                            gCAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATg     >  2:2117894/1‑101 (MQ=255)
                                                                                             cAGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATgg    <  1:2117349/101‑1 (MQ=255)
                                                                                              aGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGc   >  1:2117896/1‑101 (MQ=255)
                                                                                              aGTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGc   >  2:2117897/1‑101 (MQ=255)
                                                                                               gTCACTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGca  <  1:2117567/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
GAGCATCGCCACATGCTGACCGTTCAGACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCGCTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCA  >  NC_000913/1620709‑1620904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑