BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,640,401	T→C	E62G (GAA→GGA)	essQ ←	Qin prophage; putative S lysis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,640,401	0	T	C	100.0%	1048.9 / NA	252	E62G (GAA→GGA)	essQ	Qin prophage; putative S lysis protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (136/116); total (136/116)

Only 100 of 259 total aligned reads displayed.

TACCGTAACTTTATTCTCAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTT  >  NC_000913/1640303‑1640498

tACCGTAACTTTATTCTCAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGtctcct                                                                                                 >  1:2144198/1‑101 (MQ=255)

tACCGTAACTTTATTCTCAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGtctcct                                                                                                 >  2:2144199/1‑101 (MQ=255)

  ccGTAACTTTATTCTCAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCttt                                                                                               <  2:2143879/101‑1 (MQ=255)

  ccGTAACTTTATTCTCAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCttt                                                                                               <  1:2143941/101‑1 (MQ=255)

   cGTAACTTTATTCTCAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTa                                                                                              >  1:2144203/1‑101 (MQ=255)

   cGTAACTTTATTCTCAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTa                                                                                              <  1:2143971/101‑1 (MQ=255)

      aaCTTTATTCTCAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATc                                                                                           >  1:2144207/1‑101 (MQ=255)

       aCTTTATTCTCAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCt                                                                                          <  2:2143873/101‑1 (MQ=255)

       aCTTTATTCTCAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCt                                                                                          <  2:2143738/101‑1 (MQ=255)

       aCTTTATTCTCAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCt                                                                                          <  2:2143957/101‑1 (MQ=255)

        cTTTATTCTCAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCtt                                                                                         >  2:2144213/1‑101 (MQ=255)

        cTTTATTCTCAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCtt                                                                                         >  2:2144211/1‑101 (MQ=255)

         tttATTCTCAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTg                                                                                        <  1:2143950/101‑1 (MQ=255)

         tttATTCTCAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTg                                                                                        >  1:2144218/1‑101 (MQ=255)

         tttATTCTCAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTg                                                                                        >  1:2144214/1‑101 (MQ=255)

           tATTCTCAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGCGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGaa                                                                                      >  2:2144219/1‑101 (MQ=255)

           tATTCTCAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGaa                                                                                      <  1:2143616/101‑1 (MQ=255)

            aTTCTCAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGaaa                                                                                     <  1:2143909/101‑1 (MQ=255)

            aTTCTCAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGaaa                                                                                     >  2:2144222/1‑101 (MQ=255)

            aTTCTCAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGaaa                                                                                     <  1:2143841/101‑1 (MQ=255)

               ctcAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATaa                                                                                  <  1:2143898/101‑1 (MQ=255)

               ctcAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATaa                                                                                  <  1:2143884/101‑1 (MQ=255)

               ctcAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATaa                                                                                  <  2:2143772/101‑1 (MQ=255)

                tcAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATaaa                                                                                 >  2:2144225/1‑101 (MQ=255)

                    aaTTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGAtt                                                                             <  2:2143836/101‑1 (MQ=255)

                    aaTTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGAtt                                                                             <  2:2143945/101‑1 (MQ=255)

                    aaTTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGAtt                                                                             >  1:2144228/1‑101 (MQ=255)

                    aaTTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGAtt                                                                             >  2:2144230/1‑101 (MQ=255)

                     aTTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGAttt                                                                            <  2:2143942/101‑1 (MQ=255)

                     aTTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGAttt                                                                            <  2:2143915/101‑1 (MQ=255)

                     aTTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGAttt                                                                            >  2:2144232/1‑101 (MQ=255)

                     aTTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGAttt                                                                            <  1:2143923/101‑1 (MQ=255)

                     aTTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGAttt                                                                            <  2:2143797/101‑1 (MQ=255)

                       tACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGt                                                                          >  2:2144236/1‑101 (MQ=255)

                        aCGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTc                                                                         <  2:2143894/101‑1 (MQ=255)

                        aCGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTc                                                                         >  1:2144238/1‑101 (MQ=255)

                          ggACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAg                                                                       >  2:2144240/1‑101 (MQ=255)

                          ggACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAg                                                                       >  1:2144243/1‑101 (MQ=255)

                          ggACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAg                                                                       <  2:2143932/101‑1 (MQ=255)

                           gACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCttt                                                                                               <  2:2143646/76‑1 (MQ=255)

                             cTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATa                                                                    <  2:2143805/101‑1 (MQ=255)

                             cTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATa                                                                    >  1:2144246/1‑101 (MQ=255)

                              tCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATa                                                                    >  1:2144248/1‑100 (MQ=255)

                              tCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATAc                                                                   <  1:2143931/101‑1 (MQ=255)

                                cTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGt                                                                 <  1:2143853/101‑1 (MQ=255)

                                 tttCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATAc                                                                   >  1:2144252/1‑98 (MQ=255)

                                  ttCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTca                                                               >  1:2144254/1‑101 (MQ=255)

                                   tCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTcag                                                              >  2:2144256/1‑101 (MQ=255)

                                     acaACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTcagca                                                            <  2:2143985/101‑1 (MQ=255)

                                      caACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTcagcag                                                           >  2:2144260/1‑101 (MQ=255)

                                      caACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTca                                                               >  1:2144261/1‑97 (MQ=255)

                                         ccAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGcc                                                        >  2:2144265/1‑101 (MQ=255)

                                          cAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCa                                                       <  2:2143849/101‑1 (MQ=255)

                                             ttCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCaaaa                                                    <  2:2143903/101‑1 (MQ=255)

                                             ttCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCaaaa                                                    >  1:2144268/1‑101 (MQ=255)

                                              tCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCaaaaa                                                   >  2:2144270/1‑101 (MQ=255)

                                              tCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCaaaaa                                                   <  1:2143742/101‑1 (MQ=255)

                                               cATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAAc                                                  >  1:2144272/1‑101 (MQ=255)

                                                aTAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAAcc                                                 <  2:2143999/101‑1 (MQ=255)

                                                  aGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAg                                               >  1:2144276/1‑101 (MQ=255)

                                                   gTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAgg                                              <  2:2143820/101‑1 (MQ=255)

                                                   gTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAgg                                              >  2:2144278/1‑101 (MQ=255)

                                                   gTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAgg                                              >  1:2144279/1‑101 (MQ=255)

                                                    tCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGc                                             <  1:2143961/101‑1 (MQ=255)

                                                        gAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTAcc                                         >  1:2144284/1‑101 (MQ=255)

                                                         aGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTAccc                                        >  2:2144289/1‑101 (MQ=255)

                                                          gTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCa                                       <  1:2144021/101‑1 (MQ=255)

                                                          gTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCa                                       >  1:2144290/1‑101 (MQ=255)

                                                           tCATTGGATTACTCTCCTCTCGCAGACTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTAcc                                         >  1:2144292/1‑98 (MQ=255)

                                                             aTTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGca                                    <  2:2144042/101‑1 (MQ=255)

                                                              ttGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGcac                                   <  1:2144080/101‑1 (MQ=255)

                                                              ttGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGcac                                   >  1:2144295/1‑101 (MQ=255)

                                                               tGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGcaca                                  >  2:2144298/1‑101 (MQ=255)

                                                                 gATTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACAcc                                >  1:2144301/1‑101 (MQ=255)

                                                                  aTTACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCg                               >  2:2144302/1‑101 (MQ=255)

                                                                    tACTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGAt                             >  2:2144305/1‑101 (MQ=255)

                                                                     aCTCTCCTCTCGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGAtt                            <  1:2143983/101‑1 (MQ=255)

                                                                      ctctcctctcGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTg                           <  1:2143815/101‑1 (MQ=255)

                                                                      ctctcctctcGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTg                           >  1:2144308/1‑101 (MQ=255)

                                                                         tcctctcGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAg                        >  2:2144311/1‑101 (MQ=255)

                                                                           ctctcGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGcc                      <  1:2143926/101‑1 (MQ=255)

                                                                           ctctcGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGcc                      <  2:2144064/101‑1 (MQ=255)

                                                                             ctcGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCa                    >  1:2144313/1‑101 (MQ=255)

                                                                              tcGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCAc                   <  1:2144028/101‑1 (MQ=255)

                                                                               cGCAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACt                  >  2:2144317/1‑101 (MQ=255)

                                                                                 cAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACtgt                >  2:2144319/1‑101 (MQ=255)

                                                                                 cAGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACtgt                >  1:2144322/1‑101 (MQ=255)

                                                                                  aGCCTTACGCCTGTCTCCTTTAATCTTGAAATATAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACtgtg               <  1:2143827/101‑1 (MQ=255)

                                                                                  aGCCTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACtgtg               >  2:2144323/1‑101 (MQ=255)

                                                                                     cTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACg            <  1:2144050/101‑1 (MQ=255)

                                                                                     cTTACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACg            >  1:2144327/1‑101 (MQ=255)

                                                                                      ttACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACgg           <  2:2144284/101‑1 (MQ=255)

                                                                                      ttACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACgg           >  2:2144330/1‑101 (MQ=255)

                                                                                      ttACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACgg           >  1:2144329/1‑101 (MQ=255)

                                                                                      ttACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACgg           <  2:2144096/101‑1 (MQ=255)

                                                                                      ttACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACgg           <  2:2143935/101‑1 (MQ=255)

                                                                                       tACGCCTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGa          <  2:2144138/101‑1 (MQ=255)

                                                                                           ccTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTa      <  2:2143767/101‑1 (MQ=255)

                                                                                            cTGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTAc     >  1:2144334/1‑101 (MQ=255)

                                                                                             tGTCTCCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACt    >  2:2144337/1‑101 (MQ=255)

TACCGTAACTTTATTCTCAAAATTACGGACTCCTTTCACAACCAGTTCATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTT  >  NC_000913/1640303‑1640498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`