BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,880,710	G→A	A17V (GCC→GTC)	leuE ←	leucine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,880,710	0	G	A	99.6%	968.2 / NA	246	A17V (GCC→GTC)	leuE	leucine exporter
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (117/128); minor base C (0/1); total (117/129)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

CACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATA  >  NC_000913/1880612‑1880807

 acCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGAccc                                                                                                >  1:2452499/1‑101 (MQ=255)

  ccGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGAcccc                                                                                               >  2:2452500/1‑101 (MQ=255)

       ggCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCa                                                                                          >  1:2452504/1‑101 (MQ=255)

        gCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAg                                                                                         <  2:2452217/101‑1 (MQ=255)

          cGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGAt                                                                                       <  1:2452116/101‑1 (MQ=255)

          cGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGAt                                                                                       >  2:2452505/1‑101 (MQ=255)

          cGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGAt                                                                                       <  1:2452170/101‑1 (MQ=255)

           gCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATa                                                                                      >  1:2452509/1‑101 (MQ=255)

            cAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAg                                                                                     <  1:2452511/101‑1 (MQ=255)

                aTAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTcc                                                                                 >  1:2452513/1‑101 (MQ=255)

                aTAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTcc                                                                                 <  2:2452054/101‑1 (MQ=255)

                 tAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCa                                                                                >  2:2452514/1‑101 (MQ=255)

                   aCCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGt                                                                              >  1:2452518/1‑101 (MQ=255)

                      gCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAAt                                                                           >  1:2452521/1‑101 (MQ=255)

                      gCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAAt                                                                           <  2:2452199/101‑1 (MQ=255)

                      gCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAAt                                                                           <  2:2452003/101‑1 (MQ=255)

                        cTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTc                                                                         >  2:2452524/1‑101 (MQ=255)

                        cTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTc                                                                         <  2:2452117/101‑1 (MQ=255)

                        cTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTc                                                                         <  1:2452357/101‑1 (MQ=255)

                         tttCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCa                                                                        >  1:2452528/1‑101 (MQ=255)

                         tttCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCa                                                                        >  1:2452526/1‑101 (MQ=255)

                          ttCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAg                                                                       <  2:2451962/101‑1 (MQ=255)

                           tCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGa                                                                      >  2:2452529/1‑101 (MQ=255)

                           tCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGa                                                                      <  2:2452310/101‑1 (MQ=255)

                            cATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGaa                                                                     <  2:2452253/101‑1 (MQ=255)

                              tACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAAcc                                                                   >  1:2452532/1‑101 (MQ=255)

                                ccGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAAcccc                                                                 <  2:2452183/101‑1 (MQ=255)

                                ccGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAAcccc                                                                 >  1:2452534/1‑101 (MQ=255)

                                    tACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTAt                                                             >  1:2452538/1‑101 (MQ=255)

                                    tACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTAt                                                             >  1:2452540/1‑101 (MQ=255)

                                    tACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTAt                                                             <  1:2452127/101‑1 (MQ=255)

                                     aCTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTAtt                                                            <  2:2452192/101‑1 (MQ=255)

                                      cTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTc                                                           <  1:2452077/101‑1 (MQ=255)

                                        gACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAg                                                         >  2:2452543/1‑101 (MQ=255)

                                        gACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAg                                                         <  2:2452233/101‑1 (MQ=255)

                                          cGCTATTTTTGAGTACAAATAGGGTATTTGGCCCTGGCACCAACACAATAAAAGTGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCg                                                       <  2:2452210/101‑1 (MQ=255)

                                          cGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCg                                                       <  2:2452261/101‑1 (MQ=255)

                                           gCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTAt                                                             <  2:2452161/94‑1 (MQ=255)

                                           gCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGa                                                      <  1:2452144/101‑1 (MQ=255)

                                           gCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGa                                                      <  2:2452356/101‑1 (MQ=255)

                                           gCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGa                                                      >  2:2452545/1‑101 (MQ=255)

                                             tATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAac                                                    <  1:2452159/101‑1 (MQ=255)

                                             tATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAac                                                    >  1:2452547/1‑101 (MQ=255)

                                              aTTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAaca                                                   >  1:2452551/1‑101 (MQ=255)

                                               tttttGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAacac                                                  >  1:2452555/1‑101 (MQ=255)

                                               tttttGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAacac                                                  >  2:2452554/1‑101 (MQ=255)

                                                ttttGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAAcaca                                                 <  2:2452313/101‑1 (MQ=255)

                                                 tttGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACAt                                                <  1:2452324/101‑1 (MQ=255)

                                                    gAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTaa                                             >  1:2452558/1‑101 (MQ=255)

                                                    gAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTaa                                             <  2:2452112/101‑1 (MQ=255)

                                                    gAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTaa                                             >  2:2452559/1‑101 (MQ=255)

                                                     aGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAAc                                            >  1:2452562/1‑101 (MQ=255)

                                                       tACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACct                                          >  2:2452565/1‑101 (MQ=255)

                                                       tACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACct                                          >  2:2452568/1‑101 (MQ=255)

                                                         cAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACctct                                        <  1:2452138/101‑1 (MQ=255)

                                                          aaaCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCtt                                       >  2:2452570/1‑101 (MQ=255)

                                                            aCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTa                                     >  1:2452571/1‑101 (MQ=255)

                                                             cAGTGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCa                                                                        >  1:2452574/1‑65 (MQ=255)

                                                              aGGGTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAAt                                   <  2:2452328/101‑1 (MQ=255)

                                                               gggTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAAtt                                  >  2:2452576/1‑101 (MQ=255)

                                                               gggTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAAtt                                  <  1:2452016/101‑1 (MQ=255)

                                                               gggTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAAtt                                  <  2:2451975/101‑1 (MQ=255)

                                                                 gTATTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTAt                                <  1:2452098/101‑1 (MQ=255)

                                                                   aTTTGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCt                              >  1:2452580/1‑101 (MQ=255)

                                                                     ttGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCttt                            >  2:2452581/1‑101 (MQ=255)

                                                                      tGGCCCTTGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTc                           >  1:2452583/1‑101 (MQ=255)

                                                                      tGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTc                           <  1:2452232/101‑1 (MQ=255)

                                                                      tGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTc                           <  1:2452211/101‑1 (MQ=255)

                                                                      tGGCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTc                           <  2:2452245/101‑1 (MQ=255)

                                                                        gCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCtt                             <  2:2452284/97‑1 (MQ=255)

                                                                        gCCCTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGa                         <  2:2452041/101‑1 (MQ=255)

                                                                         cccTGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGAt                        <  2:2452153/101‑1 (MQ=255)

                                                                            tGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCAt                     >  2:2452589/1‑101 (MQ=255)

                                                                            tGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCAt                     <  1:2452333/101‑1 (MQ=255)

                                                                            tGGCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCAt                     >  2:2452587/1‑101 (MQ=255)

                                                                              gCACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATgc                   <  1:2452191/101‑1 (MQ=255)

                                                                               cACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATgcg                  >  2:2452592/1‑101 (MQ=255)

                                                                               cACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATgcg                  <  2:2452032/101‑1 (MQ=255)

                                                                               cACCAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATgcg                  <  1:2452247/101‑1 (MQ=255)

                                                                                 ccAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGcgcg                <  1:2452300/101‑1 (MQ=255)

                                                                                 ccAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGcgcg                >  2:2452594/1‑101 (MQ=255)

                                                                                 ccAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGcgcg                <  1:2452103/101‑1 (MQ=255)

                                                                                  cAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGa               <  2:2452339/101‑1 (MQ=255)

                                                                                  cAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGa               >  2:2452598/1‑101 (MQ=255)

                                                                                  cAACACAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGa               >  1:2452599/1‑101 (MQ=255)

                                                                                  cAACACAAAAAAAATGACCCCAACCAAATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGa               >  1:2452601/1‑101 (MQ=255)

                                                                                    acacAATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGAtt             <  2:2452064/101‑1 (MQ=255)

                                                                                     cacaATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTa            <  2:2452225/101‑1 (MQ=255)

                                                                                      acaATAAAAATGACCCCAACCAGATAGGTCCTGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTaa           <  1:2452218/101‑1 (MQ=255)

                                                                                      acaATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTaa           <  2:2452187/101‑1 (MQ=255)

                                                                                      acaATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTaa           <  1:2452425/101‑1 (MQ=255)

                                                                                       caATAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTaaa          >  1:2452605/1‑101 (MQ=255)

                                                                                        aaTAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAg         <  1:2452393/101‑1 (MQ=255)

                                                                                        aaTAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAg         <  2:2452287/101‑1 (MQ=255)

                                                                                        aaTAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAg         <  1:2452224/101‑1 (MQ=255)

                                                                                        aaTAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAg         >  1:2452607/1‑101 (MQ=255)

                                                                                         aTAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAgg        >  1:2452610/1‑101 (MQ=255)

                                                                                         aTAAAAATGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAgg        >  2:2452611/1‑101 (MQ=255)

                                                                                             aaaTGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGaa    >  1:2452614/1‑101 (MQ=255)

                                                                                               aTGACCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAata  <  2:2452431/101‑1 (MQ=255)

CACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATA  >  NC_000913/1880612‑1880807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`