BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,953,492	A→T	S17S (TCA→TCT)	cmoB →	tRNA U34 carboxymethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,953,492	0	A	T	100.0%	1147.8 / NA	276	S17S (TCA→TCT)	cmoB	tRNA U34 carboxymethyltransferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (146/130); total (146/130)

Only 100 of 280 total aligned reads displayed.

TGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGCGGTGGAGTTTCTAC  >  NC_000913/1953395‑1953589

tGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCtcac                                                                                                <  2:2545355/101‑1 (MQ=255)

  cTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTg                                                                                              <  1:2545491/101‑1 (MQ=255)

     tAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCt                                                                                           >  1:2545651/1‑101 (MQ=255)

     tAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCt                                                                                           >  2:2545650/1‑101 (MQ=255)

     tAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCt                                                                                           <  2:2545413/101‑1 (MQ=255)

       aCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCg                                                                                         <  2:2545185/101‑1 (MQ=255)

        cTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGa                                                                                        <  2:2545281/101‑1 (MQ=255)

          ttGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGaaa                                                                                      <  2:2545324/101‑1 (MQ=255)

          ttGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGaaa                                                                                      <  1:2545362/101‑1 (MQ=255)

            ggTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGaaa                                                                                      >  2:2545655/1‑99 (MQ=255)

             gTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGc                                                                                   >  1:2545656/1‑101 (MQ=255)

               tCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGaaa                                                                                      >  2:2545659/1‑96 (MQ=255)

                cACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGc                                                                                <  2:2545400/101‑1 (MQ=255)

                    ggtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCcgc                                                                            <  1:2545288/101‑1 (MQ=255)

                     gtggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAATTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCcgcg                                                                           >  2:2545664/1‑101 (MQ=255)

                      tggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCcgc                                                                            <  1:2545666/99‑1 (MQ=255)

                      tggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCcgc                                                                            >  2:2545666/1‑99 (MQ=255)

                       ggCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCa                                                                         <  1:2545116/101‑1 (MQ=255)

                          aTTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGAt                                                                      <  1:2545000/101‑1 (MQ=255)

                          aTTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGAt                                                                      >  1:2545673/1‑101 (MQ=255)

                          aTTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGAt                                                                      >  1:2545670/1‑101 (MQ=255)

                           ttAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGAtt                                                                     >  1:2545677/1‑101 (MQ=255)

                           ttAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGAtt                                                                     >  2:2545678/1‑101 (MQ=255)

                            tAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTg                                                                    <  1:2545148/101‑1 (MQ=255)

                            tAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTg                                                                    <  1:2545333/101‑1 (MQ=255)

                               aaGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTa                                                                 <  1:2545409/101‑1 (MQ=255)

                                 gCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAAc                                                               >  2:2545682/1‑101 (MQ=255)

                                  cAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACt                                                              <  2:2545299/101‑1 (MQ=255)

                                  cAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACt                                                              >  1:2545684/1‑101 (MQ=255)

                                  cAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACt                                                              <  2:2545314/101‑1 (MQ=255)

                                   agagGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTg                                                             >  2:2545686/1‑101 (MQ=255)

                                      ggACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGAtt                                                                     >  1:2545691/1‑90 (MQ=255)

                                       gACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAg                                                         >  2:2545692/1‑101 (MQ=255)

                                        aCGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGc                                                                   >  1:2545695/1‑90 (MQ=255)

                                          gCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGc                                                                                   >  2:2545698/1‑72 (MQ=255)

                                            tGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAgcgc                                                      >  2:2545703/1‑99 (MQ=255)

                                            tGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGa                                                    >  1:2545702/1‑101 (MQ=255)

                                            tGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGa                                                    <  2:2545307/101‑1 (MQ=255)

                                              cATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGagc                                                  >  2:2545707/1‑101 (MQ=255)

                                              cATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGagc                                                  <  1:2545296/101‑1 (MQ=255)

                                               aTGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGaaa                                                                                      >  1:2545712/1‑64 (MQ=255)

                                               aTGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGc                                                                                   >  2:2545710/1‑67 (MQ=255)

                                               aTGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGagca                                                 <  1:2545375/101‑1 (MQ=255)

                                               aTGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGagca                                                 <  1:2545452/101‑1 (MQ=255)

                                                tGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGagcag                                                <  1:2545520/101‑1 (MQ=255)

                                                 gATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGagcagc                                               <  2:2545387/101‑1 (MQ=255)

                                                 gATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGagcagc                                               <  1:2545222/101‑1 (MQ=255)

                                                  aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGagcagca                                              >  2:2545718/1‑101 (MQ=255)

                                                  aTCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGagcagca                                              >  1:2545715/1‑101 (MQ=255)

                                                   tCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGagcagcag                                             >  2:2545720/1‑101 (MQ=255)

                                                   tCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGagcagcag                                             <  2:2545204/101‑1 (MQ=255)

                                                     gACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAg                                                                        >  2:2545724/1‑72 (MQ=255)

                                                     gACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAgcagcagca                                           <  1:2545322/101‑1 (MQ=255)

                                                      aCTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCAc                                          <  1:2545429/101‑1 (MQ=255)

                                                      aCTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCAc                                          >  1:2545725/1‑101 (MQ=255)

                                                        tttGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACgg                                        <  2:2545235/101‑1 (MQ=255)

                                                        tttGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACgg                                        >  1:2545730/1‑101 (MQ=255)

                                                         ttGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACggg                                       >  2:2545732/1‑101 (MQ=255)

                                                           ggTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAg                                                                        >  2:2545737/1‑66 (MQ=255)

                                                           ggTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGgtt                                     >  1:2545736/1‑101 (MQ=255)

                                                            gTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGgttg                                    <  2:2545220/101‑1 (MQ=255)

                                                              aaCTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACggg                                       >  2:2545741/1‑96 (MQ=255)

                                                               aCTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGgttg                                    >  2:2545743/1‑98 (MQ=255)

                                                               aCTTTTATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGttt                                 <  2:2545579/101‑1 (MQ=255)

                                                                 ttttATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTaa                               <  2:2545541/101‑1 (MQ=255)

                                                                 ttttATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTaa                               <  2:2545422/101‑1 (MQ=255)

                                                                   ttATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGc                             >  2:2545747/1‑101 (MQ=255)

                                                                    tATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCa                            >  1:2545748/1‑101 (MQ=255)

                                                                    tATTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCa                            <  1:2545354/101‑1 (MQ=255)

                                                                     aTTCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAg                           <  1:2545391/101‑1 (MQ=255)

                                                                      ttCTCTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGt                          <  1:2545608/101‑1 (MQ=255)

                                                                       tctcTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTg                         <  2:2545264/101‑1 (MQ=255)

                                                                       tctcTGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTg                         >  1:2545751/1‑101 (MQ=255)

                                                                        ctctGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTgg                        <  2:2545342/101‑1 (MQ=255)

                                                                        ctctGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTgg                        >  1:2545755/1‑101 (MQ=255)

                                                                         tctGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGt                       <  1:2545341/101‑1 (MQ=255)

                                                                         tctGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGGTGTTTAAGCAg                           >  2:2545756/1‑97 (MQ=255)

                                                                          ctGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTc                      <  2:2545473/101‑1 (MQ=255)

                                                                           tGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTc                      <  2:2545377/100‑1 (MQ=255)

                                                                           tGATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGGTGttt                                 >  2:2545761/1‑89 (MQ=255)

                                                                            gATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCaa                    >  2:2545764/1‑101 (MQ=255)

                                                                            gATTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCaa                    <  1:2545544/101‑1 (MQ=255)

                                                                             aTTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCaaa                   >  1:2545765/1‑101 (MQ=255)

                                                                             aTTGCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCaaa                   <  2:2545488/101‑1 (MQ=255)

                                                                                gCCAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGc                >  2:2545769/1‑101 (MQ=255)

                                                                                 ccAAAAATCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGCg               >  1:2545771/1‑101 (MQ=255)

                                                                                   aaaaaTCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGCgg              >  1:2545775/1‑100 (MQ=255)

                                                                                    aaaaTCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGCGGTg            >  2:2545776/1‑101 (MQ=255)

                                                                                    aaaaTCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGCGGTg            <  1:2545240/101‑1 (MQ=255)

                                                                                     aaaTCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGgttg                                    >  1:2545779/1‑76 (MQ=255)

                                                                                      aaTCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGCgg              >  2:2545781/1‑97 (MQ=255)

                                                                                      aaTCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGCGGTGGa          <  1:2545370/101‑1 (MQ=255)

                                                                                      aaTCATCTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGCGGTGGa          <  2:2545461/101‑1 (MQ=255)

                                                                                        tcatcTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGCGGTGGAGt        <  1:2545478/101‑1 (MQ=255)

                                                                                         catcTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGCGGTGGAGtt       >  1:2545786/1‑101 (MQ=255)

                                                                                          atcTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGggtg                                    >  2:2545788/1‑71 (MQ=255)

                                                                                          atcTTTCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGCGGTGGAGttt      <  2:2545436/101‑1 (MQ=255)

                                                                                             tttCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGCGGTGGAGTTTCTa   <  1:2545385/101‑1 (MQ=255)

                                                                                              ttCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGCGGTGGAGTTTCTAc  >  1:2545792/1‑101 (MQ=255)

                                                                                              ttCTCACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGCGGTGGAGTTTCTAc  <  1:2545305/101‑1 (MQ=255)

TGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGCGGTGGAGTTTCTAC  >  NC_000913/1953395‑1953589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`