BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,981,422	G→A	A64V (GCC→GTC)	otsA ←	trehalose‑6‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,981,422	0	G	A	100.0%	984.0 / NA	245	A64V (GCC→GTC)	otsA	trehalose‑6‑phosphate synthase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (112/133); total (112/133)

Only 100 of 247 total aligned reads displayed.

CACCAGATCGAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAG  >  NC_000913/1981325‑1981519

 aCCAGATCGAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCa                                                                                               <  2:2580777/101‑1 (MQ=255)

 aCCAGATCGAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCa                                                                                               >  1:2581038/1‑101 (MQ=255)

  ccAGATCGAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCAc                                                                                              >  2:2581039/1‑101 (MQ=255)

   cAGATCGAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAATAGACCCACg                                                                                             >  1:2581041/1‑101 (MQ=255)

         gAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCa                                                                                               <  2:2580721/93‑1 (MQ=255)

         gAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGt                                                                                       <  1:2580869/101‑1 (MQ=255)

         gAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGt                                                                                       <  1:2580893/101‑1 (MQ=255)

          aGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGAcc                                                                                                 <  1:2580535/90‑1 (MQ=255)

          aGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACg                                                                                             <  2:2580653/94‑1 (MQ=255)

          aGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGtt                                                                                      >  2:2581045/1‑101 (MQ=255)

           gCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTa                                                                                     >  2:2581047/1‑101 (MQ=255)

              gATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATg                                                                                        <  1:2580562/95‑1 (MQ=255)

               aTAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCtt                                                                                 <  1:2580660/101‑1 (MQ=255)

               aTAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCtt                                                                                 >  2:2581051/1‑101 (MQ=255)

                 aaTGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCtttt                                                                               >  2:2581053/1‑101 (MQ=255)

                   tGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCtttttt                                                                             <  2:2580786/101‑1 (MQ=255)

                    gAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTc                                                                            >  1:2581056/1‑101 (MQ=255)

                      aaaGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCAc                                                                          <  1:2580628/101‑1 (MQ=255)

                      aaaGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCAACGTAATGTTACCtttttt                                                                             >  2:2581058/1‑98 (MQ=255)

                       aaGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCAcc                                                                         <  1:2580645/101‑1 (MQ=255)

                           gggCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCtttt                                                                     <  1:2580693/101‑1 (MQ=255)

                           gggCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCtttt                                                                     >  2:2581060/1‑101 (MQ=255)

                           gggCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCtttt                                                                     <  1:2580854/101‑1 (MQ=255)

                            ggCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCttttt                                                                    <  1:2580790/101‑1 (MQ=255)

                             gCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCtttttt                                                                   >  2:2581064/1‑101 (MQ=255)

                             gCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCtttt                                                                     <  1:2481074/99‑1 (MQ=255)

                              ccAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTa                                                                  <  1:2580772/101‑1 (MQ=255)

                              ccAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTa                                                                  >  2:2581066/1‑101 (MQ=255)

                               cAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAg                                                                 <  2:2580455/101‑1 (MQ=255)

                               cAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAg                                                                 >  2:2581069/1‑101 (MQ=255)

                                agagAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGc                                                                >  1:2581070/1‑101 (MQ=255)

                                 gagaACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCg                                                               <  2:2580565/101‑1 (MQ=255)

                                 gagaACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCg                                                               <  1:2580614/101‑1 (MQ=255)

                                  agaACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCgg                                                              >  2:2581073/1‑101 (MQ=255)

                                  agaACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCgg                                                              <  1:2580397/101‑1 (MQ=255)

                                  agaACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCgg                                                              <  1:2580640/101‑1 (MQ=255)

                                    aaCGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCt                                                            >  1:2581077/1‑101 (MQ=255)

                                    aaCGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCt                                                            >  2:2581079/1‑101 (MQ=255)

                                    aaCGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCt                                                            <  2:2580817/101‑1 (MQ=255)

                                    aaCGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCt                                                            <  2:2580670/101‑1 (MQ=255)

                                     aCGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTg                                                           <  1:2580669/101‑1 (MQ=255)

                                      cGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGa                                                          >  1:2581081/1‑101 (MQ=255)

                                      cGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGa                                                          <  2:2580631/101‑1 (MQ=255)

                                        gCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATc                                                        <  1:2580605/101‑1 (MQ=255)

                                         cATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATcc                                                       >  1:2581085/1‑101 (MQ=255)

                                         cATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATcc                                                       >  2:2581084/1‑101 (MQ=255)

                                           ttGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTc                                                     >  1:2581087/1‑101 (MQ=255)

                                            tGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCa                                                    <  1:2580910/101‑1 (MQ=255)

                                              gagaATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTa                                                                  >  2:2581090/1‑85 (MQ=255)

                                               agaATTGGTTGTAGTATTCGTCAAAGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCt                                                      <  1:2580680/96‑1 (MQ=255)

                                                gaATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCAtt                                                  >  2:2581094/1‑99 (MQ=255)

                                                gaATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTcc                                                <  2:2580704/101‑1 (MQ=255)

                                                  aTTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCt                                              <  1:2580700/101‑1 (MQ=255)

                                                  aTTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCt                                              >  2:2581096/1‑101 (MQ=255)

                                                    tGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGt                                            <  1:2580462/101‑1 (MQ=255)

                                                     ggTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGtt                                           >  1:2581099/1‑101 (MQ=255)

                                                      gTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGttt                                          >  2:2581100/1‑101 (MQ=255)

                                                        tGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTca                                        >  2:2581103/1‑101 (MQ=255)

                                                        tGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTca                                        <  2:2580846/101‑1 (MQ=255)

                                                         gtagtaTTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTcac                                       >  1:2581104/1‑101 (MQ=255)

                                                         gtagtaTTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTcac                                       <  2:2580747/101‑1 (MQ=255)

                                                            gtaTTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCtttt                                                                     >  2:2581109/1‑68 (MQ=255)

                                                              aTTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTc                                  >  1:2581111/1‑101 (MQ=255)

                                                               ttCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGttt                                          >  2:2581113/1‑92 (MQ=255)

                                                                tCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCa                                <  2:2580729/101‑1 (MQ=255)

                                                                tCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCa                                <  1:2580731/101‑1 (MQ=255)

                                                                tCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCa                                <  1:2580757/101‑1 (MQ=255)

                                                                  gTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGc                              <  1:2580745/101‑1 (MQ=255)

                                                                    cAAGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCa                            >  1:2581116/1‑101 (MQ=255)

                                                                      aGGTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCaaa                          >  2:2581117/1‑101 (MQ=255)

                                                                       ggTCCTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAAc                         <  2:2580888/101‑1 (MQ=255)

                                                                          ccTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACcac                      <  1:2580722/101‑1 (MQ=255)

                                                                           cTGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACcaca                     >  1:2581120/1‑101 (MQ=255)

                                                                            tGTTCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCaa                           >  2:2581123/1‑94 (MQ=255)

                                                                               tCGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCCCCACTCCAGCCAAACCACAGTcc                 <  2:2581035/101‑1 (MQ=255)

                                                                                cGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCg                <  1:2580655/101‑1 (MQ=255)

                                                                                cGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCg                >  1:2581125/1‑101 (MQ=255)

                                                                                cGCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCg                <  2:2580572/101‑1 (MQ=255)

                                                                                 gCTGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGc               <  1:2580811/101‑1 (MQ=255)

                                                                                   tGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCt             >  2:2581127/1‑101 (MQ=255)

                                                                                   tGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCt             <  2:2580949/101‑1 (MQ=255)

                                                                                   tGAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCt             <  2:2580829/101‑1 (MQ=255)

                                                                                    gAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTg            >  2:2581130/1‑101 (MQ=255)

                                                                                    gAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTg            <  1:2580941/101‑1 (MQ=255)

                                                                                    gAGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTg            <  1:2580809/101‑1 (MQ=255)

                                                                                     aGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGc           >  1:2581134/1‑101 (MQ=255)

                                                                                     aGGTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCACTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGc           <  1:2580593/101‑1 (MQ=255)

                                                                                      ggTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCg          <  2:2580882/101‑1 (MQ=255)

                                                                                      ggTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCg          <  2:2580609/101‑1 (MQ=255)

                                                                                      ggTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCg          <  1:2580737/101‑1 (MQ=255)

                                                                                      ggTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCg          <  1:2580686/101‑1 (MQ=255)

                                                                                       gTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCgg         >  1:2581136/1‑101 (MQ=255)

                                                                                       gTTAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCgg         >  2:2581137/1‑101 (MQ=255)

                                                                                        ttAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGc        <  2:2580954/101‑1 (MQ=255)

                                                                                         tAAAAGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCt       >  2:2581140/1‑101 (MQ=255)

                                                                                          aaaaGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCtt      >  1:2581142/1‑101 (MQ=255)

                                                                                            aaGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTc    >  2:2581147/1‑101 (MQ=255)

                                                                                            aaGAGACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTc    >  1:2581145/1‑101 (MQ=255)

                                                                                             agagACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCa   <  1:2580760/101‑1 (MQ=255)

                                                                                             agagACCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCa   >  2:2581149/1‑101 (MQ=255)

CACCAGATCGAGCCGATAATGAAAAGCGGGCCAGAGAACGGCATTGGAGAATTGGTTGTAGTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAG  >  NC_000913/1981325‑1981519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`