Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,130,945 T→C T376A (ACC→GCC)  wcaC ← putative colanic acid biosynthesis glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,130,9450TC99.6% 950.9 / ‑4.5 236T376A (ACC→GCC) wcaCputative colanic acid biosynthesis glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (109/126);  total (109/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

Only 100 of 236 total aligned reads displayed.

AGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCAACACT  >  NC_000913/2130847‑2131042
                                                                                                  |                                                                                                 
   tAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCaa                                                                                              >  1:2773659/1‑101 (MQ=255)
     gCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAat                                                                                            >  2:2773660/1‑101 (MQ=255)
     gCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAat                                                                                            <  2:2773439/101‑1 (MQ=255)
      cTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAata                                                                                           <  2:2773394/101‑1 (MQ=255)
       tACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAatat                                                                                          <  2:2773241/101‑1 (MQ=255)
       tACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAatat                                                                                          <  1:2773279/101‑1 (MQ=255)
       tACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAatat                                                                                          >  1:2773663/1‑101 (MQ=255)
          aGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAatat                                                                                          <  2:2773138/98‑1 (MQ=255)
          aGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGc                                                                                       >  1:2773668/1‑101 (MQ=255)
            aTTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCt                                                                                     >  1:2773671/1‑101 (MQ=255)
             ttCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTg                                                                                    >  1:2773673/1‑101 (MQ=255)
              tCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTgc                                                                                   >  2:2773677/1‑101 (MQ=255)
              tCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTgc                                                                                   >  1:2773676/1‑101 (MQ=255)
               cTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTgcg                                                                                  <  2:2773260/101‑1 (MQ=255)
               cTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTgcg                                                                                  <  1:2773335/101‑1 (MQ=255)
               cTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTgcg                                                                                  >  2:2773682/1‑101 (MQ=255)
               cTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTgcg                                                                                  >  1:2773681/1‑101 (MQ=255)
                tGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTgcgc                                                                                 <  1:2773379/101‑1 (MQ=255)
                 gATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGcgcg                                                                                >  2:2773687/1‑101 (MQ=255)
                  aTAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGa                                                                               >  1:2773691/1‑101 (MQ=255)
                  aTAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGa                                                                               <  1:2773039/101‑1 (MQ=255)
                  aTAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGa                                                                               >  2:2773690/1‑101 (MQ=255)
                   tAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGAt                                                                              <  2:2773292/101‑1 (MQ=255)
                    aGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGAtt                                                                             >  1:2773695/1‑101 (MQ=255)
                     gAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGAttt                                                                            >  1:2773698/1‑101 (MQ=255)
                     gAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGAttt                                                                            <  1:2773218/101‑1 (MQ=255)
                     gAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGAttt                                                                            <  2:2773308/101‑1 (MQ=255)
                     gAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGAttt                                                                            <  1:2773341/101‑1 (MQ=255)
                      aaGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTc                                                                           <  2:2773375/101‑1 (MQ=255)
                      aaGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTc                                                                           <  1:2773312/101‑1 (MQ=255)
                       aGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTcc                                                                          >  2:2773701/1‑101 (MQ=255)
                         ttGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCgg                                                                        >  2:2773704/1‑101 (MQ=255)
                          tGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGt                                                                       >  1:2773707/1‑101 (MQ=255)
                            aCATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGa                                                                               >  2:2773710/1‑91 (MQ=255)
                             cATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTg                                                                    >  2:2773712/1‑101 (MQ=255)
                               tACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAata                                                                                           <  2:2773269/76‑1 (MQ=255)
                               tACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCt                                                                  <  1:2773228/101‑1 (MQ=255)
                               tACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCt                                                                  >  1:2773713/1‑101 (MQ=255)
                                aCTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCtt                                                                 <  1:2773372/101‑1 (MQ=255)
                                   cctccAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAAc                                                              >  1:2773715/1‑101 (MQ=255)
                                    ctccAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACt                                                             <  1:2773640/101‑1 (MQ=255)
                                    ctccAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACt                                                             <  1:2773533/101‑1 (MQ=255)
                                     tccAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTg                                                            >  2:2773719/1‑101 (MQ=255)
                                     tccAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTg                                                            <  1:2773158/101‑1 (MQ=255)
                                      ccAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGc                                                           <  2:2773402/101‑1 (MQ=255)
                                      ccAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGc                                                           >  1:2773722/1‑101 (MQ=255)
                                       cAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCa                                                          >  2:2773723/1‑101 (MQ=255)
                                        aGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCAc                                                         <  2:2773349/101‑1 (MQ=255)
                                        aGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCAc                                                         >  1:2773725/1‑101 (MQ=255)
                                          cATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCa                                                       >  1:2773728/1‑101 (MQ=255)
                                          cATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCa                                                       <  2:2773281/101‑1 (MQ=255)
                                          cATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCa                                                       <  2:2773355/101‑1 (MQ=255)
                                           aTCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTAGCACCaaa                                                                                             <  2:2773044/62‑1 (MQ=255)
                                            tCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGt                                                     >  1:2773731/1‑101 (MQ=255)
                                             cTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGtt                                                    <  1:2773465/101‑1 (MQ=255)
                                              tgttgtCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTg                                                   >  2:2773732/1‑101 (MQ=255)
                                               gttgtCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTgc                                                  <  1:2773406/101‑1 (MQ=255)
                                                  gtCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTgcagc                                               >  1:2773736/1‑101 (MQ=255)
                                                   tCCACTGTAGGCGGCGCGGCTGCGTTGTCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTgcagca                                              <  1:2773420/101‑1 (MQ=255)
                                                   tCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTgcagca                                              >  2:2773737/1‑101 (MQ=255)
                                                   tCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTgcagca                                              <  2:2773180/101‑1 (MQ=255)
                                                    ccACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCAc                                             <  1:2773266/101‑1 (MQ=255)
                                                     cACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCgg                                                                        <  2:2773159/73‑1 (MQ=255)
                                                     cACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTg                                                                    <  2:2773442/77‑1 (MQ=255)
                                                     cACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCAcc                                            >  2:2773739/1‑101 (MQ=255)
                                                       cTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACctc                                          >  1:2773742/1‑101 (MQ=255)
                                                        tGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACctct                                         <  2:2773368/101‑1 (MQ=255)
                                                        tGTAGGCGGCGCAGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACctct                                         <  1:2773385/101‑1 (MQ=255)
                                                          tAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCtcttc                                       <  1:2773250/101‑1 (MQ=255)
                                                          tAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCtcttc                                       <  1:2773361/101‑1 (MQ=255)
                                                            ggcggcGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTcttctt                                     <  2:2773346/101‑1 (MQ=255)
                                                            ggcggcGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTcttctt                                     >  2:2773743/1‑101 (MQ=255)
                                                              cggcgcggcTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCg                                   <  1:2773445/101‑1 (MQ=255)
                                                              cggcgcggcTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCg                                   <  1:2773315/101‑1 (MQ=255)
                                                                gcgcggcTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCt                                 <  2:2773518/101‑1 (MQ=255)
                                                                 cgcggcTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTg                                <  1:2773433/101‑1 (MQ=255)
                                                                 cgcggcTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTg                                <  1:2773479/101‑1 (MQ=255)
                                                                  gcggcTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGa                               <  1:2773524/101‑1 (MQ=255)
                                                                    ggcTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACg                             >  1:2773749/1‑101 (MQ=255)
                                                                     gcTGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACgg                            >  2:2773750/1‑101 (MQ=255)
                                                                       tGCGTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGtt                          >  2:2773752/1‑101 (MQ=255)
                                                                          gTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTa                       <  1:2773326/101‑1 (MQ=255)
                                                                          gTTGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTa                       >  1:2773755/1‑101 (MQ=255)
                                                                            tGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTAcc                     >  1:2773758/1‑101 (MQ=255)
                                                                            tGGCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTAcc                     <  1:2773283/101‑1 (MQ=255)
                                                                             ggCTGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTAccg                    <  2:2773421/101‑1 (MQ=255)
                                                                               gtGAACTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTAccgcc                  >  1:2773760/2‑101 (MQ=255)
                                                                                  aaCTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCgg                <  2:2773482/100‑1 (MQ=255)
                                                                                    cTCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGAtt             <  2:2773311/101‑1 (MQ=255)
                                                                                     tCAGCCAGCGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGAttt            <  1:2773573/101‑1 (MQ=255)
                                                                                      cagccagcGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGAtttt           >  1:2773763/1‑101 (MQ=255)
                                                                                       agccagcGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGAttttt          >  1:2773770/1‑101 (MQ=255)
                                                                                       agccagcGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGAttttt          >  1:2773767/1‑101 (MQ=255)
                                                                                       agccagcGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGAttttt          >  2:2773766/1‑101 (MQ=255)
                                                                                           agcGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCaa      >  2:2773771/1‑101 (MQ=255)
                                                                                             cGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCAaca    <  2:2773747/101‑1 (MQ=255)
                                                                                             cGTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCAaca    <  1:2773295/101‑1 (MQ=255)
                                                                                              gTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCAacac   <  2:2773462/101‑1 (MQ=255)
                                                                                              gTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCAacac   <  2:2773319/101‑1 (MQ=255)
                                                                                              gTGGCACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCAacac   <  1:2773390/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
AGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCAACACT  >  NC_000913/2130847‑2131042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑