BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	674,408	C→T	A126T (GCA→ACA)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,408	0	C	T	83.9%	1058.0 / 170.4	360	A126T (GCA→ACA)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base C (26/32); new base T (150/152); total (176/184)
Fisher's exact test for biased strand distribution p-value = 5.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01

Only 100 of 361 total aligned reads displayed.

CCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTT  >  NC_000913/674310‑674507

ccAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCa                                                                                                   <  2:1111811/101‑1 (MQ=255)

ccAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCa                                                                                                   >  1:1112613/1‑101 (MQ=255)

    ttGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTc                                                                                               <  2:1112041/101‑1 (MQ=255)

    ttGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTc                                                                                               <  1:1112193/101‑1 (MQ=255)

     tGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTcg                                                                                              >  1:1112618/1‑101 (MQ=255)

        ccGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCgg                                                                                           <  1:1112422/101‑1 (MQ=255)

         cGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGc                                                                                          >  1:1112624/1‑101 (MQ=255)

          gCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCt                                                                                         <  1:1112400/101‑1 (MQ=255)

          gCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCt                                                                                         >  1:1112627/1‑101 (MQ=255)

             gAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCa                                                                                      <  2:1112245/101‑1 (MQ=255)

              aaGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAg                                                                                     >  1:1112633/1‑101 (MQ=255)

               aGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGt                                                                                    <  1:1112117/101‑1 (MQ=255)

                gTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTc                                                                                   >  1:1112637/1‑101 (MQ=255)

                gTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTc                                                                                   >  2:1112639/1‑101 (MQ=255)

                 tcttctTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCa                                                                                  <  1:1112163/101‑1 (MQ=255)

                 tcttctTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCa                                                                                  <  1:1112213/101‑1 (MQ=255)

                  cttcttATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCAt                                                                                 <  2:1112118/101‑1 (MQ=255)

                   ttcttATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATa                                                                                >  2:1112644/1‑101 (MQ=255)

                   ttcttATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATa                                                                                <  1:1112282/101‑1 (MQ=255)

                    tcttATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATaa                                                                               <  1:1112177/101‑1 (MQ=255)

                      ttATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAAcc                                                                             >  2:1112650/1‑101 (MQ=255)

                       tatataCCAGGCCTTTTTTATACAGCTCGGTCAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCa                                                                            <  2:1112154/101‑1 (MQ=255)

                        atataCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCaa                                                                           >  2:1112654/1‑101 (MQ=255)

                        atataCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCaa                                                                           >  1:1112656/1‑101 (MQ=255)

                          ataCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAg                                                                         <  2:1112129/101‑1 (MQ=255)

                          ataCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAg                                                                         >  2:1112660/1‑101 (MQ=255)

                           taCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGc                                                                        <  1:1112234/101‑1 (MQ=255)

                           taCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGc                                                                        >  2:1112664/1‑101 (MQ=255)

                              cAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCa                                                                     <  2:1112269/101‑1 (MQ=255)

                               aGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAg                                                                    >  2:1112671/1‑101 (MQ=255)

                               aGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAg                                                                    >  1:1112670/1‑101 (MQ=255)

                               aGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAg                                                                    <  1:1112047/101‑1 (MQ=255)

                                ggCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTcgcg                                                                                            <  1:1111876/76‑1 (MQ=255)

                                ggCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGc                                                                   <  1:1112248/101‑1 (MQ=255)

                                 gCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCa                                                                  <  1:1112310/101‑1 (MQ=255)

                                  ccTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCAt                                                                 <  1:1112028/101‑1 (MQ=255)

                                  ccTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCAt                                                                 <  2:1112301/101‑1 (MQ=255)

                                     tttttATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCAtttt                                                              <  2:1112229/101‑1 (MQ=255)

                                     tttttATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCAtttt                                                              >  2:1112677/1‑101 (MQ=255)

                                     tttttATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCAtttt                                                              >  1:1112678/1‑101 (MQ=255)

                                       tttATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCAtttt                                                              >  2:1112686/1‑99 (MQ=255)

                                       tttATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGa                                                            <  1:1112305/101‑1 (MQ=255)

                                       tttATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGa                                                            <  1:1112360/101‑1 (MQ=255)

                                       tttATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGa                                                            <  2:1112013/101‑1 (MQ=255)

                                       tttATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGa                                                            <  2:1111926/101‑1 (MQ=255)

                                       tttATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGa                                                            >  1:1112685/1‑101 (MQ=255)

                                          ataCAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCt                                                         >  2:1112691/1‑101 (MQ=255)

                                           taCAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTg                                                        >  1:1112694/1‑101 (MQ=255)

                                            aCAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTgg                                                       >  2:1112697/1‑101 (MQ=255)

                                            aCAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTgg                                                       <  1:1112063/101‑1 (MQ=255)

                                              aGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGtt                                                     >  2:1112704/1‑101 (MQ=255)

                                               gCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGc                                                                        <  1:1112137/81‑1 (MQ=255)

                                               gCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGttt                                                    >  1:1112705/1‑101 (MQ=255)

                                                cTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGtttt                                                   <  2:1112236/101‑1 (MQ=255)

                                                 tCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGttttt                                                  >  2:1112711/1‑101 (MQ=255)

                                                 tCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGttttt                                                  <  1:1112297/101‑1 (MQ=255)

                                                  cGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTc                                                 <  2:1112383/101‑1 (MQ=255)

                                                    gTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCat                                               <  2:1112097/101‑1 (MQ=255)

                                                    gTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCat                                               >  1:1112714/1‑101 (MQ=255)

                                                       aagaaTTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCAtata                                            >  1:1112718/1‑101 (MQ=255)

                                                         gaaTTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATAcg                                          >  2:1112722/1‑101 (MQ=255)

                                                         gaaTTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATAcg                                          <  1:1112519/101‑1 (MQ=255)

                                                           aTTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATAcgcg                                        >  1:1112724/1‑101 (MQ=255)

                                                            tttCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGa                                       >  2:1112726/1‑101 (MQ=255)

                                                             ttCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGAt                                      <  2:1112589/101‑1 (MQ=255)

                                                             ttCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCACAGCATTTTGAGCTGGTTTTTCATATACGCGAt                                      >  1:1112731/1‑101 (MQ=255)

                                                               cTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCata                                              <  2:1112173/91‑1 (MQ=255)

                                                                tGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGAtgtt                                   <  2:1112628/101‑1 (MQ=255)

                                                                  ttCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGAtgttgt                                 >  2:1112736/1‑101 (MQ=255)

                                                                   tCCCAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTc                                >  1:1112738/1‑101 (MQ=255)

                                                                    cccAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCg                               >  2:1112741/1‑101 (MQ=255)

                                                                     ccAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGt                              >  2:1112744/1‑101 (MQ=255)

                                                                      cAACGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTa                             <  1:1112379/101‑1 (MQ=255)

                                                                         cGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGt                          >  2:1112750/1‑101 (MQ=255)

                                                                         cGGTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGt                          <  1:1112206/101‑1 (MQ=255)

                                                                          ggTAGTATTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTc                         >  2:1112753/1‑101 (MQ=255)

                                                                            tagtaTTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCa                       >  1:1112757/1‑101 (MQ=255)

                                                                             agtaTTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCAc                      <  2:1112329/101‑1 (MQ=255)

                                                                             agtaTTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCAc                      <  2:1112489/101‑1 (MQ=255)

                                                                              gtaTTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACg                     <  2:1112347/101‑1 (MQ=255)

                                                                              gtaTTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACg                     <  2:1112188/101‑1 (MQ=255)

                                                                               taTTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACgg                    >  2:1112762/1‑101 (MQ=255)

                                                                               taTTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACgg                    >  1:1112761/1‑101 (MQ=255)

                                                                               taTTCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACgg                    <  2:1112202/101‑1 (MQ=255)

                                                                                 ttCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTg                  >  2:1112766/1‑101 (MQ=255)

                                                                                 ttCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTg                  <  1:1112274/101‑1 (MQ=255)

                                                                                  tCCGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGc                 <  2:1112539/101‑1 (MQ=255)

                                                                                   ccGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCAc                      >  1:1112772/1‑95 (MQ=255)

                                                                                    cGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCg               <  2:1112433/101‑1 (MQ=255)

                                                                                    cGGCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCg               >  2:1112774/1‑101 (MQ=255)

                                                                                     ggCGTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCgg              <  2:1112293/101‑1 (MQ=255)

                                                                                        gTACAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGc           >  1:1112777/1‑101 (MQ=255)

                                                                                          aCAGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCgg         >  1:1112781/1‑101 (MQ=255)

                                                                                          aCAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCgg         <  2:1112356/101‑1 (MQ=255)

                                                                                            aGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGgtg       >  2:1112786/1‑101 (MQ=255)

                                                                                            aGGTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGgtg       >  1:1112788/1‑101 (MQ=255)

                                                                                            aGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGgtg       <  1:1112258/101‑1 (MQ=255)

                                                                                              gTTGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGtgtt     <  1:1112100/101‑1 (MQ=255)

                                                                                               ttGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGtgttg    >  1:1112794/1‑101 (MQ=255)

                                                                                               ttGTCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGtgttg    <  1:1112352/101‑1 (MQ=255)

CCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTT  >  NC_000913/674310‑674507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`