BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	94,771	T→C	V41A (GTT→GCT)	murF →	D‑alanyl‑D‑alanine‑adding enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	94,771	0	T	C	100.0%	1184.5 / NA	282	V41A (GTT→GCT)	murF	D‑alanyl‑D‑alanine‑adding enzyme
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (173/109); total (173/109)

Only 100 of 297 total aligned reads displayed.

ACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATC  >  NC_000913/94673‑94868

aCTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTttgctg                                                                                                 >  2:159315/1‑101 (MQ=255)

  ttACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGcc                                                                                               <  2:158800/101‑1 (MQ=255)

  ttACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGcc                                                                                               >  2:159321/1‑101 (MQ=255)

  ttACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGcc                                                                                               >  1:159318/1‑101 (MQ=255)

    aCCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCt                                                                                             <  1:158701/101‑1 (MQ=255)

    aCCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCt                                                                                             >  2:159326/1‑101 (MQ=255)

    aCCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCt                                                                                             >  2:159329/1‑101 (MQ=255)

     ccGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTg                                                                                            <  2:158690/101‑1 (MQ=255)

     ccGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTg                                                                                            >  1:159330/1‑101 (MQ=255)

        aCATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGaaa                                                                                         <  1:158959/101‑1 (MQ=255)

        aCATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGaaa                                                                                         <  1:158974/101‑1 (MQ=255)

        aCATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGaaa                                                                                         >  1:159334/1‑101 (MQ=255)

          aTTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAgg                                                                                       >  2:159338/1‑101 (MQ=255)

           ttCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGc                                                                                      <  2:158998/101‑1 (MQ=255)

            tctcAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCg                                                                                     >  1:159339/1‑101 (MQ=255)

            tctcAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCg                                                                                     <  2:158842/101‑1 (MQ=255)

             ctcAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGa                                                                                    <  2:158915/101‑1 (MQ=255)

               cAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAAc                                                                                  <  2:158770/101‑1 (MQ=255)

               cAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAAc                                                                                  >  1:159343/1‑101 (MQ=255)

                 aCGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGt                                                                                >  2:159346/1‑101 (MQ=255)

                  cGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGtt                                                                               >  2:159349/1‑101 (MQ=255)

                   ggTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGttt                                                                              <  1:159101/101‑1 (MQ=255)

                    gTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGtttt                                                                             >  1:159353/1‑101 (MQ=255)

                    gTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGtttt                                                                             <  2:159016/101‑1 (MQ=255)

                     tGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTg                                                                            >  2:159354/1‑101 (MQ=255)

                       aaCTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGc                                                                                      >  1:159358/1‑89 (MQ=255)

                         cTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGc                                                                        >  2:159360/1‑101 (MQ=255)

                           gCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGccc                                                                      >  2:159364/1‑101 (MQ=255)

                            cAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCa                                                                     >  2:159367/1‑101 (MQ=255)

                             aaGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCAc                                                                    >  2:159370/1‑101 (MQ=255)

                             aaGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCAc                                                                    >  1:159373/1‑101 (MQ=255)

                              aGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACg                                                                   >  2:159375/1‑101 (MQ=255)

                                gTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGAt                                                                 >  2:159379/1‑101 (MQ=255)

                                   cAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGAtttt                                                              <  1:158804/101‑1 (MQ=255)

                                   cAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGAtttt                                                              >  1:159381/1‑101 (MQ=255)

                                     gATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGc                                                            >  1:159385/1‑101 (MQ=255)

                                      atatCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGcc                                                           <  1:158852/101‑1 (MQ=255)

                                       tatCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCg                                                          <  1:158780/101‑1 (MQ=255)

                                       tatCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCg                                                          >  1:159389/1‑101 (MQ=255)

                                          cACCCTTGGTGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACATTTTGATGCCCACGATTTTGCCGAcc                                                       >  1:159393/1‑101 (MQ=255)

                                          cACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGAcc                                                       >  2:159394/1‑101 (MQ=255)

                                           aCCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCa                                                      <  1:159167/101‑1 (MQ=255)

                                            cccTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCa                                                                     <  1:158922/85‑1 (MQ=255)

                                             ccTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAgg                                                    >  1:159400/1‑101 (MQ=255)

                                             ccTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAgg                                                    >  2:159399/1‑101 (MQ=255)

                                              cTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGc                                                   >  1:159404/1‑101 (MQ=255)

                                              cTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGc                                                   <  2:158870/101‑1 (MQ=255)

                                                tGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGa                                                 >  1:159408/1‑101 (MQ=255)

                                                 gATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGaa                                                >  2:159410/1‑101 (MQ=255)

                                                  aTGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTg                                                             >  1:159414/1‑87 (MQ=255)

                                                  aTGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGaaa                                               >  1:159411/1‑101 (MQ=255)

                                                  aTGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGaaa                                               <  2:158896/101‑1 (MQ=255)

                                                    gCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGc                                             >  2:159417/1‑101 (MQ=255)

                                                    gCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGc                                             <  1:158888/101‑1 (MQ=255)

                                                      tGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTg                                           <  1:158867/101‑1 (MQ=255)

                                                      tGTAACCACTGATACCCGAAAACTGACGCCGGACTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTg                                           >  2:159421/1‑101 (MQ=255)

                                                       gTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTgg                                          >  1:159422/1‑101 (MQ=255)

                                                        tAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTggc                                         <  1:159014/101‑1 (MQ=255)

                                                         aaCCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTggcg                                        >  2:159427/1‑101 (MQ=255)

                                                         aaCCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTggcg                                        <  2:159123/101‑1 (MQ=255)

                                                          aCCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTggcgg                                       >  1:159429/1‑101 (MQ=255)

                                                          aCCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTggcgg                                       <  2:158961/101‑1 (MQ=255)

                                                           ccACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTggcggc                                      <  1:159249/101‑1 (MQ=255)

                                                           ccACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTggcggc                                      <  1:159026/101‑1 (MQ=255)

                                                           ccACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTggcggc                                      >  2:159431/1‑101 (MQ=255)

                                                            cACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGgcggcg                                     >  2:159436/1‑101 (MQ=255)

                                                               tGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCggcgcag                                  >  1:159439/1‑101 (MQ=255)

                                                                 aTACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCAc                                                                    >  2:159446/1‑65 (MQ=255)

                                                                 aTACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCggcgcaggc                                >  2:159442/1‑101 (MQ=255)

                                                                 aTACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCggcgcaggc                                >  1:159443/1‑101 (MQ=255)

                                                                  tACCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCggcgcaggcg                               <  1:158901/101‑1 (MQ=255)

                                                                   aCCCGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCggcgcaggcgc                              >  2:159450/1‑101 (MQ=255)

                                                                     ccGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAgg                                                    <  1:158943/77‑1 (MQ=255)

                                                                      cGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAgg                                                    >  1:159453/1‑76 (MQ=255)

                                                                      cGAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCact                           <  2:158931/101‑1 (MQ=255)

                                                                       gAAAACTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCacta                          >  1:159457/1‑101 (MQ=255)

                                                                        aaaaCTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCactac                         <  2:159088/101‑1 (MQ=255)

                                                                         aaaCTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCactact                        >  2:159460/1‑101 (MQ=255)

                                                                          aaCTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTg                       <  1:159007/101‑1 (MQ=255)

                                                                          aaCTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTg                       <  2:159300/101‑1 (MQ=255)

                                                                           aCTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTgg                      >  2:159464/1‑101 (MQ=255)

                                                                           aCTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTgg                      <  2:158953/101‑1 (MQ=255)

                                                                           aCTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTgg                      >  1:159463/1‑101 (MQ=255)

                                                                            cTGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGt                     <  2:158979/101‑1 (MQ=255)

                                                                             tGACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGtt                    >  2:159468/1‑101 (MQ=255)

                                                                              gACGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTa                   <  1:158832/101‑1 (MQ=255)

                                                                               aCGCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAg                  <  1:159056/101‑1 (MQ=255)

                                                                                 gCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGcc                >  1:159472/1‑101 (MQ=255)

                                                                                 gCCGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGcc                <  2:159071/101‑1 (MQ=255)

                                                                                  ccGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCg               >  2:159474/1‑101 (MQ=255)

                                                                                   cGGGCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGt              >  2:159477/1‑101 (MQ=255)

                                                                                    gggCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTc             >  2:159480/1‑101 (MQ=255)

                                                                                     ggCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTcc            >  1:159482/1‑101 (MQ=255)

                                                                                     ggCTGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTcc            <  2:159135/101‑1 (MQ=255)

                                                                                        tGCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCt         >  1:159487/1‑101 (MQ=255)

                                                                                         gCCTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTg        >  2:159488/1‑101 (MQ=255)

                                                                                           cTGTTTGCTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGa      >  1:159491/1‑101 (MQ=255)

                                                                                            tgtttgctGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGAc     >  2:159495/1‑101 (MQ=255)

                                                                                              tttgctGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACAt   >  1:159498/1‑101 (MQ=255)

                                                                                              tttgctGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACAt   <  2:159039/101‑1 (MQ=255)

ACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATC  >  NC_000913/94673‑94868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`