BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,195,359	T→C	G79G (GGT→GGC)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,195,359	0	T	C	99.7%	1240.3 / ‑3.9	299	G79G (GGT→GGC)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (153/145); total (154/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.58e-01

Only 100 of 299 total aligned reads displayed.

AAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTC  >  NC_000913/1195261‑1195456

aaaGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCa                                                                                                 >  2:1944953/1‑101 (MQ=255)

aaaGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCa                                                                                                 >  1:1944955/1‑101 (MQ=255)

aaaGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCa                                                                                                 >  1:1944951/1‑101 (MQ=255)

 aaGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAg                                                                                                <  2:1944494/101‑1 (MQ=255)

 aaGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAg                                                                                                <  1:1944580/101‑1 (MQ=255)

    tGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGAc                                                                                             <  1:1944322/101‑1 (MQ=255)

    tGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGAc                                                                                             >  1:1944959/1‑101 (MQ=255)

     ggTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACg                                                                                            <  1:1944563/101‑1 (MQ=255)

      gTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGt                                                                                           >  2:1944963/1‑101 (MQ=255)

       tCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTc                                                                                          >  2:1944967/1‑101 (MQ=255)

       tCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTc                                                                                          <  1:1944623/101‑1 (MQ=255)

        cGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCt                                                                                         >  1:1944970/1‑101 (MQ=255)

         gACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTg                                                                                        >  2:1944971/1‑101 (MQ=255)

         gACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTg                                                                                        <  1:1944651/101‑1 (MQ=255)

         gACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTg                                                                                        >  2:1944974/1‑101 (MQ=255)

          aCGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTgg                                                                                       >  1:1944977/1‑101 (MQ=255)

           cGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGc                                                                                      >  1:1944980/1‑101 (MQ=255)

           cGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGc                                                                                      <  1:1944733/101‑1 (MQ=255)

            gCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGct                                                                                     >  2:1944982/1‑101 (MQ=255)

            gCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGct                                                                                     <  2:1944612/101‑1 (MQ=255)

            gCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGct                                                                                     <  1:1944636/101‑1 (MQ=255)

            gCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGct                                                                                     <  2:1944454/101‑1 (MQ=255)

             cTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGctg                                                                                    >  2:1944985/1‑101 (MQ=255)

                 aGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGctgcctg                                                                                >  1:1944989/1‑101 (MQ=255)

                  gTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGctgcctgc                                                                               >  2:1944990/1‑101 (MQ=255)

                     gagaAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGa                                                                            >  1:1944995/1‑101 (MQ=255)

                     gagaAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGa                                                                            <  2:1944557/101‑1 (MQ=255)

                        aaaGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAAc                                                                         >  2:1944999/1‑101 (MQ=255)

                          aGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAActc                                                                       >  1:1945001/1‑101 (MQ=255)

                          aGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAActc                                                                       <  1:1944482/101‑1 (MQ=255)

                           gCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAACATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAActct                                                                      >  2:1945003/1‑101 (MQ=255)

                              tataAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTg                                                                    >  2:1945008/1‑100 (MQ=255)

                              tataAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGa                                                                   <  1:1944662/101‑1 (MQ=255)

                               ataAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGAt                                                                  >  1:1945009/1‑101 (MQ=255)

                                taAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCGCACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATc                                                                 <  2:1944677/101‑1 (MQ=255)

                                taAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATc                                                                 >  2:1945012/1‑101 (MQ=255)

                                taAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATc                                                                 <  1:1944510/101‑1 (MQ=255)

                                 aaaGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCt                                                                <  1:1944592/101‑1 (MQ=255)

                                   aGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGa                                                              <  1:1944751/101‑1 (MQ=255)

                                   aGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGa                                                              <  1:1944428/101‑1 (MQ=255)

                                    ggCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGAt                                                             <  1:1944699/101‑1 (MQ=255)

                                     gCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGAtt                                                            >  2:1945017/1‑101 (MQ=255)

                                        aGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGt                                                         >  2:1945020/1‑101 (MQ=255)

                                           gTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGaa                                                      >  1:1945021/1‑101 (MQ=255)

                                            tAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGAc                                                                                             <  1:1944609/61‑1 (MQ=255)

                                             aaaaTCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAata                                                    >  2:1945025/1‑101 (MQ=255)

                                               aaTCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATc                                                  >  2:1945030/1‑101 (MQ=255)

                                                aTCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATcg                                                 >  1:1945032/1‑101 (MQ=255)

                                                aTCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATcg                                                 <  1:1944149/101‑1 (MQ=255)

                                                   tcCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGt                                              <  1:1944694/101‑1 (MQ=255)

                                                    ccTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGt                                                         <  2:1944466/89‑1 (MQ=255)

                                                    ccTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGtt                                             >  2:1945035/1‑101 (MQ=255)

                                                    ccTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGtt                                             <  1:1944602/101‑1 (MQ=255)

                                                     cTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTg                                            >  2:1945039/1‑101 (MQ=255)

                                                      tggatggaAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGc                                           >  1:1945040/1‑101 (MQ=255)

                                                       ggatggaAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGcc                                          <  2:1944529/101‑1 (MQ=255)

                                                       ggatggaAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGcc                                          <  2:1944643/101‑1 (MQ=255)

                                                        gatggaAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCa                                         <  1:1944551/101‑1 (MQ=255)

                                                         atggaAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCAt                                        <  1:1944556/101‑1 (MQ=255)

                                                         atggaAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCAt                                        >  1:1945044/1‑101 (MQ=255)

                                                         atggaAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCAt                                        >  2:1945045/1‑101 (MQ=255)

                                                          tggaAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCAtt                                       <  1:1944630/101‑1 (MQ=255)

                                                            gaAATTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTaa                                     >  1:1945049/1‑101 (MQ=255)

                                                             aaaTTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTaaa                                    >  1:1945055/1‑101 (MQ=255)

                                                             aaaTTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTaaa                                    >  2:1945052/1‑101 (MQ=255)

                                                              aaTTTACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAg                                   <  1:1944574/101‑1 (MQ=255)

                                                                tttACACCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTaaa                                    >  2:1945057/1‑98 (MQ=255)

                                                                    cacCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGAt                                                             <  1:1944619/69‑1 (MQ=255)

                                                                    cacCGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGc                             >  2:1945061/1‑101 (MQ=255)

                                                                     acCGGTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCt                            >  1:1945065/1‑101 (MQ=255)

                                                                      ccGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAg                                   <  1:1944588/93‑1 (MQ=255)

                                                                      ccGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTg                           >  2:1945069/1‑101 (MQ=255)

                                                                      ccGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTg                           >  1:1945068/1‑101 (MQ=255)

                                                                       cGGTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGa                          <  1:1944445/101‑1 (MQ=255)

                                                                         gTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGAcc                        >  1:1945075/1‑101 (MQ=255)

                                                                         gTGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGAcc                        >  2:1945074/1‑101 (MQ=255)

                                                                          tGAAAAATCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCa                       <  2:1944756/101‑1 (MQ=255)

                                                                            aaaaaTCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACt                     >  2:1945080/1‑101 (MQ=255)

                                                                            aaaaaTCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACt                     >  1:1945081/1‑101 (MQ=255)

                                                                              aaaTCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTcc                   <  1:1944541/101‑1 (MQ=255)

                                                                              aaaTCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTcc                   <  1:1944670/101‑1 (MQ=255)

                                                                              aaaTCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTcc                   >  2:1945084/1‑101 (MQ=255)

                                                                               aaTCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCg                  <  2:1944714/101‑1 (MQ=255)

                                                                                aTCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCt                            <  1:1944676/90‑1 (MQ=255)

                                                                                aTCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCgg                 <  1:1944833/101‑1 (MQ=255)

                                                                                aTCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCgg                 >  1:1945088/1‑101 (MQ=255)

                                                                                 tCCACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGt                <  2:1944664/101‑1 (MQ=255)

                                                                                  ccACACAGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGtt               >  2:1945091/1‑101 (MQ=255)

                                                                                     cacaGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTtggt            >  1:1945093/1‑101 (MQ=255)

                                                                                     cacaGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTtggt            <  1:1944497/101‑1 (MQ=255)

                                                                                       caGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTtggtgg          <  2:1944638/101‑1 (MQ=255)

                                                                                       caGGTTTATGGCCAGGACGTCTGGCTGCCTGCAGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTtggtgg          >  2:1945098/1‑101 (MQ=255)

                                                                                        aGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTtggt            <  1:1944462/98‑1 (MQ=255)

                                                                                        aGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGc         >  2:1945102/1‑101 (MQ=255)

                                                                                        aGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGc         <  2:1944813/101‑1 (MQ=255)

                                                                                        aGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGc         <  1:1944706/101‑1 (MQ=255)

                                                                                        aGGTTTATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGc         >  1:1945100/1‑101 (MQ=255)

                                                                                           tttATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGt      <  1:1944777/101‑1 (MQ=255)

                                                                                             tATGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTAt    >  1:1945107/1‑101 (MQ=255)

                                                                                              aTGGCCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTAtt   <  2:1944573/101‑1 (MQ=255)

AAAGTGGTCGACGCTGCAGTCGAGAAAGCCTATAAAGGCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTC  >  NC_000913/1195261‑1195456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`