BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	127,249	A→G	I519V (ATC→GTC)	aceF →	pyruvate dehydrogenase, E2 subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	127,249	0	A	G	100.0%	1175.0 / NA	284	I519V (ATC→GTC)	aceF	pyruvate dehydrogenase, E2 subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (120/164); total (120/164)

Only 100 of 299 total aligned reads displayed.

AGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCACCATCTC  >  NC_000913/127151‑127346

aGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGcgtc                                                                                                 >  1:212927/1‑101 (MQ=255)

  cGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGCATTCAAAGACGTCAACAAGAAAGGcgtcat                                                                                               >  2:212930/1‑101 (MQ=255)

   gTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCgtcatc                                                                                              <  1:212628/101‑1 (MQ=255)

   gTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCgtcatc                                                                                              <  1:212466/101‑1 (MQ=255)

     cTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGa                                                                                            >  1:212933/1‑101 (MQ=255)

      tGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAg                                                                                           <  2:212599/101‑1 (MQ=255)

      tGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAg                                                                                           <  1:212407/101‑1 (MQ=255)

       gACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGc                                                                                          >  1:212939/1‑101 (MQ=255)

        aCCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCt                                                                                         >  2:212941/1‑101 (MQ=255)

             gaagaaATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGtctc                                                                                    >  1:212946/1‑101 (MQ=255)

             gaagaaATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGtctc                                                                                    <  1:212330/101‑1 (MQ=255)

             gaagaaATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGtctc                                                                                    <  1:212655/101‑1 (MQ=255)

               agaaATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTcgc                                                                                  <  2:212368/101‑1 (MQ=255)

               agaaATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTcgc                                                                                  <  1:212425/101‑1 (MQ=255)

               agaaATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTcgc                                                                                  >  2:212950/1‑101 (MQ=255)

                 aaaTACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGa                                                                                >  1:212951/1‑101 (MQ=255)

                   aTACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGc                                                                              >  1:212954/1‑101 (MQ=255)

                     aCATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCtg                                                                            <  2:212702/101‑1 (MQ=255)

                     aCATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCtg                                                                            <  1:212280/101‑1 (MQ=255)

                      cATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGtctc                                                                                    <  2:212441/92‑1 (MQ=255)

                          aaCATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGAc                                                                       >  1:212958/1‑101 (MQ=255)

                              tCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTAtt                                                                   >  2:212963/1‑101 (MQ=255)

                              tCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTAtt                                                                   <  1:212669/101‑1 (MQ=255)

                               cGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTAttt                                                                  <  1:212442/101‑1 (MQ=255)

                               cGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTAttt                                                                  <  1:212371/101‑1 (MQ=255)

                                ggTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTc                                                                 <  1:212610/101‑1 (MQ=255)

                                ggTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTc                                                                 >  1:212964/1‑101 (MQ=255)

                                 gtgtGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCt                                                                <  1:212523/101‑1 (MQ=255)

                                   gtgGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTaa                                                              >  2:212970/1‑101 (MQ=255)

                                   gtgGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTaa                                                              <  1:212585/101‑1 (MQ=255)

                                   gtgGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTaa                                                              <  2:212414/101‑1 (MQ=255)

                                   gtgGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTaa                                                              >  1:212967/1‑101 (MQ=255)

                                    tgGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAg                                                             >  1:212974/1‑101 (MQ=255)

                                      gCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGc                                                                                          <  1:212448/70‑1 (MQ=255)

                                       cGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAg                                                                                           <  1:212491/68‑1 (MQ=255)

                                        ggTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTg                                                                                        >  2:212979/1‑70 (MQ=255)

                                        ggTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAg                                                         <  2:212800/101‑1 (MQ=255)

                                         gTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTaa                                                              <  1:212701/95‑1 (MQ=255)

                                         gTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAgc                                                        <  1:212253/101‑1 (MQ=255)

                                         gTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAgc                                                        >  1:212980/1‑101 (MQ=255)

                                          tGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGcgtca                                                                                                <  1:212503/60‑1 (MQ=255)

                                             aTACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGt                                                    >  1:212985/1‑101 (MQ=255)

                                             aTACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGt                                                    >  2:212986/1‑101 (MQ=255)

                                              tACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTg                                                   >  1:212989/1‑101 (MQ=255)

                                              tACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTg                                                   <  2:212560/101‑1 (MQ=255)

                                                ccccGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGAc                                                 >  1:212992/1‑101 (MQ=255)

                                                   cGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGt                                              <  2:212485/101‑1 (MQ=255)

                                                   cGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGt                                              <  1:212616/101‑1 (MQ=255)

                                                    gAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTa                                             <  2:212476/101‑1 (MQ=255)

                                                    gAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTa                                             <  1:212554/101‑1 (MQ=255)

                                                    gAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTa                                             <  1:212573/101‑1 (MQ=255)

                                                     aaCGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGa                                                  <  2:212634/95‑1 (MQ=255)

                                                     aaCGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTaa                                            >  2:212997/1‑101 (MQ=255)

                                                     aaCGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTaa                                            <  2:212845/101‑1 (MQ=255)

                                                     aaCGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTaa                                            >  1:212999/1‑101 (MQ=255)

                                                       cGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGc                                          <  2:212524/101‑1 (MQ=255)

                                                        ggTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCt                                         >  2:213004/1‑101 (MQ=255)

                                                        ggTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCt                                         <  2:212695/101‑1 (MQ=255)

                                                        ggTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCt                                         >  1:213003/1‑101 (MQ=255)

                                                         gTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTg                                        <  2:212496/101‑1 (MQ=255)

                                                            tGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACt                                     <  2:212617/101‑1 (MQ=255)

                                                            tGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACt                                     <  1:212730/101‑1 (MQ=255)

                                                              gttgttCCGGTATTCAAAGACGTCAACAAGATAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGc                                   <  1:212344/101‑1 (MQ=255)

                                                              gttgttCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGc                                   <  1:212511/101‑1 (MQ=255)

                                                              gttgttCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGc                                   >  1:213009/1‑101 (MQ=255)

                                                               ttgttCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCg                                  <  1:212694/101‑1 (MQ=255)

                                                                tgttCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCgg                                 <  2:212658/101‑1 (MQ=255)

                                                                tgttCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCgg                                 <  2:212583/101‑1 (MQ=255)

                                                                 gttCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCggg                                <  2:212592/101‑1 (MQ=255)

                                                                  ttCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGc                                   >  2:213013/1‑97 (MQ=255)

                                                                  ttCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGc                               >  1:213012/1‑101 (MQ=255)

                                                                   tCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCg                              <  2:212735/101‑1 (MQ=255)

                                                                   tCCGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCg                              <  2:212568/101‑1 (MQ=255)

                                                                    ccGGTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGa                             >  1:213017/1‑101 (MQ=255)

                                                                       gTATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCggg                                <  2:212393/95‑1 (MQ=255)

                                                                        tATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATg                         >  1:213021/1‑101 (MQ=255)

                                                                        tATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATg                         <  2:212535/101‑1 (MQ=255)

                                                                        tATTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATg                         <  1:212864/101‑1 (MQ=255)

                                                                         aTTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGc                        >  2:213022/1‑101 (MQ=255)

                                                                         aTTCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGc                        <  1:212828/101‑1 (MQ=255)

                                                                          ttCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCa                       >  1:213025/1‑101 (MQ=255)

                                                                           tCAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAg                      >  1:213028/1‑101 (MQ=255)

                                                                            cAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAgg                     >  2:213032/1‑101 (MQ=255)

                                                                            cAAAGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAgg                     <  1:212546/101‑1 (MQ=255)

                                                                             aaaGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGGCTGCgg                                 >  1:213034/1‑88 (MQ=255)

                                                                              aaGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGc                        <  2:212327/96‑1 (MQ=255)

                                                                               aGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCg                  <  2:212527/101‑1 (MQ=255)

                                                                               aGACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCg                  <  1:212774/101‑1 (MQ=255)

                                                                                gACGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCgg                 <  2:212548/101‑1 (MQ=255)

                                                                                 aCGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGt                <  1:212121/101‑1 (MQ=255)

                                                                                 aCGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGt                >  1:213038/1‑101 (MQ=255)

                                                                                  cGTCAACAAGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCgg                 >  2:213040/1‑99 (MQ=255)

                                                                                      aacaaGAAAGGCGTCATCGAGCTGTCTCGCGAGCtgat                                                                          >  2:213045/1‑38 (MQ=255)

                                                                                       acaaGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCt            >  1:213046/1‑99 (MQ=255)

                                                                                       acaaGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTc          <  2:212605/101‑1 (MQ=255)

                                                                                         aaGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCAc        >  1:213050/1‑101 (MQ=255)

                                                                                          aGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCAcc       <  2:212642/101‑1 (MQ=255)

                                                                                          aGAAAGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCAcc       >  2:213053/1‑101 (MQ=255)

                                                                                            aaaGGCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCACCAt     >  1:213056/1‑101 (MQ=255)

                                                                                               ggCGTCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCACCAtctc  <  1:212641/101‑1 (MQ=255)

AGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCACCATCTC  >  NC_000913/127151‑127346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`