BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,358,224	C→T	D322N (GAC→AAC)	puuP ←	putrescine:H(+) symporter PuuP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,358,224	0	C	T	100.0%	968.5 / NA	227	D322N (GAC→AAC)	puuP	putrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (111/116); total (111/116)

Only 100 of 227 total aligned reads displayed.

CGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTG  >  NC_000913/1358126‑1358320

cgcCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTAc                                                                                                <  1:2204668/101‑1 (MQ=255)

 gcCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACg                                                                                               >  1:2205175/1‑101 (MQ=255)

  ccGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGc                                                                                              >  2:2205177/1‑101 (MQ=255)

    gACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGccc                                                                                            >  1:2205179/1‑101 (MQ=255)

    gACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGccc                                                                                            <  2:2204773/101‑1 (MQ=255)

    gACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGc                                                                                              <  2:2204702/99‑1 (MQ=255)

      cAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCa                                                                                          >  1:2205183/1‑101 (MQ=255)

      cAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCa                                                                                          >  2:2205182/1‑101 (MQ=255)

      cAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCa                                                                                          <  1:2204759/101‑1 (MQ=255)

       aaCGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCAt                                                                                         >  1:2205188/1‑101 (MQ=255)

          gcgACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATcac                                                                                      >  1:2205190/1‑101 (MQ=255)

          gcgACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATcac                                                                                      <  1:2204834/101‑1 (MQ=255)

          gcgACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATcac                                                                                      <  2:2204480/101‑1 (MQ=255)

            gACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACAt                                                                                    <  1:2204543/101‑1 (MQ=255)

             aCAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATa                                                                                   >  1:2205192/1‑101 (MQ=255)

             aCAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATa                                                                                   <  1:2204936/101‑1 (MQ=255)

              cAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATAc                                                                                  <  2:2204595/101‑1 (MQ=255)

              cAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATAc                                                                                  >  2:2205196/1‑101 (MQ=255)

              cAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATAc                                                                                  <  1:2204636/101‑1 (MQ=255)

              cAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATAc                                                                                  >  1:2205194/1‑101 (MQ=255)

               aaTCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATAca                                                                                 <  2:2204863/101‑1 (MQ=255)

                aTCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATAcag                                                                                <  2:2204909/101‑1 (MQ=255)

                 tCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATAcagc                                                                               >  2:2205199/1‑101 (MQ=255)

                     gACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGAc                                                                           >  2:2205203/1‑101 (MQ=255)

                     gACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGAc                                                                           <  2:2204899/101‑1 (MQ=255)

                      aCCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGAcg                                                                          >  2:2205205/1‑101 (MQ=255)

                      aCCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGAcg                                                                          >  1:2205206/1‑101 (MQ=255)

                       ccATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGAcgc                                                                         <  1:2204794/101‑1 (MQ=255)

                        cATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGAcgcg                                                                        <  2:2204609/101‑1 (MQ=255)

                        cATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGAcgcg                                                                        >  1:2205208/1‑101 (MQ=255)

                         aTAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGa                                                                       >  2:2205213/1‑101 (MQ=255)

                         aTAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGa                                                                       >  1:2205212/1‑101 (MQ=255)

                           aaTGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGaca                                                                     >  1:2205215/1‑101 (MQ=255)

                           aaTGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGaca                                                                     >  2:2205218/1‑101 (MQ=255)

                            aTGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGacac                                                                    >  1:2205219/1‑101 (MQ=255)

                            aTGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGacac                                                                    <  2:2204808/101‑1 (MQ=255)

                              gACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGc                                                                  <  2:2204741/101‑1 (MQ=255)

                              gACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGc                                                                  <  1:2204898/101‑1 (MQ=255)

                                cGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTg                                                                >  1:2205223/1‑101 (MQ=255)

                                cGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTg                                                                >  2:2205225/1‑101 (MQ=255)

                                  ttGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGc                                                              <  1:2204987/101‑1 (MQ=255)

                                   tGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGTCCCATCACATACAGCAGACGCGACACGCTGGCa                                                             >  1:2205227/1‑101 (MQ=255)

                                   tGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCa                                                             <  2:2205101/101‑1 (MQ=255)

                                    gAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCAt                                                            >  1:2205229/1‑101 (MQ=255)

                                    gAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCAt                                                            <  1:2204644/101‑1 (MQ=255)

                                       tGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCAtgtg                                                         >  2:2205232/1‑101 (MQ=255)

                                         ccGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGaa                                                       >  2:2205235/1‑101 (MQ=255)

                                         ccGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGaa                                                       <  1:2204846/101‑1 (MQ=255)

                                           ggAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAgc                                                     >  1:2205238/1‑101 (MQ=255)

                                           ggAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAgc                                                     <  1:2204973/101‑1 (MQ=255)

                                             aGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAgcca                                                   >  1:2205240/1‑101 (MQ=255)

                                             aGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAgcca                                                   <  1:2204784/101‑1 (MQ=255)

                                              gTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCt                                                                 <  1:2204581/86‑1 (MQ=255)

                                              gTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAgccag                                                  >  2:2205241/1‑101 (MQ=255)

                                                 cGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAgccaggcc                                               >  2:2205244/1‑101 (MQ=255)

                                                 cGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAgccaggcc                                               >  1:2205245/1‑101 (MQ=255)

                                                    cATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCaga                                            >  2:2205248/1‑101 (MQ=255)

                                                    cATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCaga                                            >  2:2205250/1‑101 (MQ=255)

                                                      ttttGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATAcagca                                                                              <  2:2204672/65‑1 (MQ=255)

                                                      ttttGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAgg                                          <  2:2204770/101‑1 (MQ=255)

                                                        ttGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCt                                        <  2:2204781/101‑1 (MQ=255)

                                                         tGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTa                                       <  2:2204748/101‑1 (MQ=255)

                                                          gggTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTaa                                      <  1:2204734/101‑1 (MQ=255)

                                                          gggTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTaa                                      <  2:2204829/101‑1 (MQ=255)

                                                           ggTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAAc                                     <  2:2204985/101‑1 (MQ=255)

                                                           ggTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAAc                                     <  2:2205044/101‑1 (MQ=255)

                                                           ggTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAAc                                     <  2:2204477/101‑1 (MQ=255)

                                                            gTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACg                                    >  1:2205254/1‑101 (MQ=255)

                                                            gTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACg                                    <  2:2204996/101‑1 (MQ=255)

                                                             tGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACgt                                   <  2:2204845/101‑1 (MQ=255)

                                                             tGCACATAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACgt                                   <  1:2204769/101‑1 (MQ=255)

                                                                acaTAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGtt                                >  1:2205258/1‑101 (MQ=255)

                                                                acaTAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGtt                                >  2:2205255/1‑101 (MQ=255)

                                                                 caTAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTc                               >  2:2205259/1‑101 (MQ=255)

                                                                 caTAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTc                               <  2:2204935/101‑1 (MQ=255)

                                                                   tAGCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTcac                             >  1:2205261/1‑101 (MQ=255)

                                                                     gCCAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACaa                           <  1:2204856/101‑1 (MQ=255)

                                                                       cAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAAc                         <  1:2204776/101‑1 (MQ=255)

                                                                       cAAACACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAAc                         >  1:2205264/1‑101 (MQ=255)

                                                                        aaaCACGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACg                        >  2:2205266/1‑101 (MQ=255)

                                                                           cacGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACaa                           <  2:2204726/95‑1 (MQ=255)

                                                                           cacGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACgtgg                     >  1:2205269/1‑101 (MQ=255)

                                                                            acGCGCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACgtggt                    <  2:2205033/101‑1 (MQ=255)

                                                                             cgcgCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACgtggtg                   >  1:2205271/1‑101 (MQ=255)

                                                                             cgcgCTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACgtggtg                   <  1:2204907/101‑1 (MQ=255)

                                                                              gcgcTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAAc                         >  1:2205274/1‑94 (MQ=255)

                                                                              gcgcTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGc                  >  2:2205272/1‑101 (MQ=255)

                                                                                 cTCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCaga               >  2:2205277/1‑101 (MQ=255)

                                                                                  tCCGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCagag              <  1:2204799/101‑1 (MQ=255)

                                                                                   ccGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAgg             <  2:2204963/101‑1 (MQ=255)

                                                                                   ccGGAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAgg             >  1:2205280/1‑101 (MQ=255)

                                                                                     ggAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGaa           >  2:2205281/1‑101 (MQ=255)

                                                                                      gAAACACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGaaa          <  2:2204737/101‑1 (MQ=255)

                                                                                       aaaCACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTcac                             >  2:2205283/1‑81 (MQ=255)

                                                                                       aaaCACATTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGaaaa         <  2:2204857/101‑1 (MQ=255)

                                                                                            caTTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGAc    <  2:2204761/101‑1 (MQ=255)

                                                                                            caTTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGAc    <  1:2205030/101‑1 (MQ=255)

                                                                                            caTTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGAc    <  1:2205057/101‑1 (MQ=255)

                                                                                            caTTGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGAc    >  1:2205288/1‑101 (MQ=255)

                                                                                              ttGTTACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTg  <  1:2204879/101‑1 (MQ=255)

CGCCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTG  >  NC_000913/1358126‑1358320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`