BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,977,978	T→C	T74A (ACG→GCG)	flhD ←	DNA‑binding transcriptional dual regulator FlhD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,977,978	0	T	C	100.0%	1363.1 / NA	328	T74A (ACG→GCG)	flhD	DNA‑binding transcriptional dual regulator FlhD
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (167/161); total (167/161)

Only 100 of 328 total aligned reads displayed.

CTGATTAACATCATTCAGCAAGCGTGTTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGTCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAACGTTGTCGCCATT  >  NC_000913/1977880‑1978075

cTGATTAACATCATTCAGCAAGCGTGTTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCt                                                                                                 >  2:3217870/1‑101 (MQ=255)

cTGATTAACATCATTCAGCAAGCGTGTTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCt                                                                                                 >  1:3217872/1‑101 (MQ=255)

  gATTAACATCATTCAGCAAGCGTGTTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCtgg                                                                                               >  1:3217875/1‑101 (MQ=255)

     tAACATCATTCAGCAAGCGTGTTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCtggtgg                                                                                            >  2:3217881/1‑101 (MQ=255)

      aaCATCATTCAGCAAGCGTGTTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGc                                                                                           >  2:3217886/1‑101 (MQ=255)

      aaCATCATTCAGCAAGCGTGTTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGc                                                                                           >  1:3217884/1‑101 (MQ=255)

          tcatTCAGCAAGCGTGTTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTc                                                                                       >  2:3217892/1‑101 (MQ=255)

          tcatTCAGCAAGCGTGTTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTc                                                                                       <  2:3217475/101‑1 (MQ=255)

           catTCAGCAAGCGTGTTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCa                                                                                      <  2:3217126/101‑1 (MQ=255)

           catTCAGCAAGCGTGTTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCa                                                                                      >  1:3217893/1‑101 (MQ=255)

            atTCAGCAAGCGTGTTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGCAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCaa                                                                                     <  1:3217280/101‑1 (MQ=255)

             ttCAGCAAGCGTGTTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCaaa                                                                                    <  1:3217399/101‑1 (MQ=255)

              tCAGCAAGCGTGTTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCaaac                                                                                   <  2:3217309/101‑2 (MQ=255)

                 gCAAGCGTGTTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACgg                                                                                >  2:3217899/1‑101 (MQ=255)

                   aaGCGTGTTGAGAGCATGATGCCGGTATTAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGaa                                                                              <  2:3217304/101‑1 (MQ=255)

                   aaGCGTGTTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGaa                                                                              <  1:3217221/101‑1 (MQ=255)

                    aGCGTGTTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAg                                                                             >  2:3217903/1‑101 (MQ=255)

                    aGCGTGTTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAg                                                                             >  1:3217902/1‑101 (MQ=255)

                       gtgtTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGa                                                                          <  1:3217521/101‑1 (MQ=255)

                       gtgtTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGa                                                                          <  1:3217566/101‑1 (MQ=255)

                       gtgtTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGa                                                                          >  2:3217908/1‑101 (MQ=255)

                       gtgtTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGa                                                                          <  1:3217614/101‑1 (MQ=255)

                        tgtTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGAc                                                                         >  2:3217913/1‑101 (MQ=255)

                        tgtTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGAc                                                                         <  1:3217323/101‑1 (MQ=255)

                        tgtTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGAc                                                                         >  1:3217910/1‑101 (MQ=255)

                          ttGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACaa                                                                       <  1:3217365/101‑1 (MQ=255)

                          ttGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACaa                                                                       >  2:3217918/1‑101 (MQ=255)

                          ttGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACaa                                                                       >  1:3217919/1‑101 (MQ=255)

                           tGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACaaa                                                                      <  2:3217192/101‑1 (MQ=255)

                           tGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACaaa                                                                      <  2:3217397/101‑1 (MQ=255)

                            gagaGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAAc                                                                     <  1:3217076/101‑1 (MQ=255)

                            gagaGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAAc                                                                     >  2:3217925/1‑101 (MQ=255)

                              gagCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCa                                                                   <  2:3217586/101‑1 (MQ=255)

                              gagCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCa                                                                   <  1:3217433/101‑1 (MQ=255)

                              gagCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCa                                                                   >  1:3217930/1‑101 (MQ=255)

                              gagCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCa                                                                   <  2:3217370/101‑1 (MQ=255)

                               agCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAg                                                                  >  2:3217931/1‑101 (MQ=255)

                               agCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAg                                                                  <  1:3217479/101‑1 (MQ=255)

                                gCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGt                                                                 >  1:3217934/1‑101 (MQ=255)

                                  atgatgCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTg                                                               >  2:3217939/1‑101 (MQ=255)

                                  atgatgCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTg                                                               >  1:3217940/1‑101 (MQ=255)

                                    gatgCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGAt                                                             >  2:3217943/1‑101 (MQ=255)

                                     atgCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAg                                                                             <  1:3217580/84‑1 (MQ=255)

                                     atgCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGAtt                                                            <  1:3217533/101‑1 (MQ=255)

                                      tgCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTg                                                           <  2:3217264/101‑1 (MQ=255)

                                          ggTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGttt                                                       >  1:3217949/1‑101 (MQ=255)

                                          ggTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGttt                                                       <  2:3217500/101‑1 (MQ=255)

                                            tATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGAc                                                                         >  2:3217952/1‑81 (MQ=255)

                                            tATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCt                                                     <  1:3217449/101‑1 (MQ=255)

                                            tATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCt                                                     <  2:3217420/101‑1 (MQ=255)

                                             aTGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTg                                                    >  1:3217954/1‑101 (MQ=255)

                                             aTGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTg                                                    <  2:3217241/101‑1 (MQ=255)

                                              tGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGc                                                   >  2:3217958/1‑101 (MQ=255)

                                              tGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGc                                                   <  1:3217464/101‑1 (MQ=255)

                                               gAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGcc                                                  >  1:3217961/1‑101 (MQ=255)

                                                aaTTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCa                                                 >  1:3217965/1‑101 (MQ=255)

                                                 aTTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAg                                                <  1:3217334/101‑1 (MQ=255)

                                                  tttGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGc                                               >  2:3217969/1‑101 (MQ=255)

                                                   ttGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCt                                              >  1:3217970/1‑101 (MQ=255)

                                                   ttGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCt                                              <  1:3217489/101‑1 (MQ=255)

                                                    tGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCaaaa                                                                                   >  2:3217975/1‑63 (MQ=255)

                                                    tGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTg                                                           <  1:3217352/87‑1 (MQ=255)

                                                    tGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGAtt                                             <  2:3217620/101‑1 (MQ=255)

                                                      cTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTaa                                           >  1:3217977/1‑101 (MQ=255)

                                                      cTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTaa                                           <  1:3217438/101‑1 (MQ=255)

                                                       tGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCtgg                                                                                               >  2:3217980/1‑48 (MQ=255)

                                                       tGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAAc                                          <  1:3217392/101‑1 (MQ=255)

                                                         gagaTCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCa                                        <  2:3217335/101‑1 (MQ=255)

                                                         gagaTCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCa                                        <  2:3217443/101‑1 (MQ=255)

                                                          agaTCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCAt                                       <  2:3217452/101‑1 (MQ=255)

                                                           gaTCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCAtt                                      >  1:3217985/1‑101 (MQ=255)

                                                            aTCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCAttt                                     >  2:3217986/1‑101 (MQ=255)

                                                             tCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTg                                    <  2:3217387/101‑1 (MQ=255)

                                                             tCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTg                                    <  1:3217253/101‑1 (MQ=255)

                                                               gTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCg                                  >  2:3217991/1‑101 (MQ=255)

                                                               gTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCg                                  >  1:3217993/1‑101 (MQ=255)

                                                                 cAACGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGa                                >  1:3217997/1‑101 (MQ=255)

                                                                   aCGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAag                              >  2:3218001/1‑101 (MQ=255)

                                                                   aCGCGGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAag                              <  1:3217511/101‑1 (MQ=255)

                                                                     gcgGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAagag                            <  2:3217540/101‑1 (MQ=255)

                                                                      cgGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGt                           >  1:3218005/1‑101 (MQ=255)

                                                                      cgGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGt                           <  2:3217576/101‑1 (MQ=255)

                                                                      cgGGAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGt                           >  2:3218006/1‑101 (MQ=255)

                                                                        ggAATCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCa                         <  2:3217314/101‑1 (MQ=255)

                                                                           aTCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTg                      >  1:3218011/1‑101 (MQ=255)

                                                                            tCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGc                     >  2:3218013/1‑101 (MQ=255)

                                                                            tCTTGCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGc                     >  1:3218017/1‑101 (MQ=255)

                                                                                gCGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCt                 >  1:3218020/1‑101 (MQ=255)

                                                                                 cGTCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTa                <  2:3217523/101‑1 (MQ=255)

                                                                                   tCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAAc              <  1:3217303/101‑1 (MQ=255)

                                                                                   tCAACTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAAc              >  2:3218022/1‑101 (MQ=255)

                                                                                      aCTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTCGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAACGtt           >  2:3218027/1‑101 (MQ=255)

                                                                                       cTGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAACGTTg          <  1:3217413/101‑1 (MQ=255)

                                                                                        tGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAACGTTGt         <  2:3217653/101‑1 (MQ=255)

                                                                                        tGAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAACGTTGt         >  1:3218031/1‑101 (MQ=255)

                                                                                         gAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAACGTTGTc        <  2:3217728/101‑1 (MQ=255)

                                                                                         gAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAACGTTGTc        <  1:3217369/101‑1 (MQ=255)

                                                                                         gAGTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAACGTTGTc        >  2:3218032/1‑101 (MQ=255)

                                                                                           gTAATCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAACGTTGTCGc      >  2:3218035/1‑101 (MQ=255)

                                                                                             aaTCGCCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAACGTTGTCGCCa    <  1:3217690/101‑1 (MQ=255)

CTGATTAACATCATTCAGCAAGCGTGTTGAGAGCATGATGCCGGTATGAATTTGCTGGAGATCGTCAACGCGGGAATCTTGCGTCAACTGAGTAATCGTCTGGTGGCTGTCAAAACGGAAGTGACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAACGTTGTCGCCATT  >  NC_000913/1977880‑1978075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`