Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,100,028 T→C N414S (AAC→AGC)  gnd ← 6‑phosphogluconate dehydrogenase, decarboxylating

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,100,0280TC100.0% 1326.9 / NA 317N414S (AAC→AGC) gnd6‑phosphogluconate dehydrogenase, decarboxylating
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (134/183);  total (134/183)

Only 100 of 320 total aligned reads displayed.

AGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGG  >  NC_000913/2099930‑2100125
                                                                                                  |                                                                                                 
aGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTc                                                                                                 >  2:3419072/1‑101 (MQ=255)
  tCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTg                                                                                               >  1:3419073/1‑101 (MQ=255)
   cACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGt                                                                                              >  2:3419077/1‑101 (MQ=255)
    aCGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTa                                                                                             >  1:3419079/1‑101 (MQ=255)
      gCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACt                                                                                           <  1:3418089/101‑1 (MQ=255)
      gCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACt                                                                                           <  1:3418472/101‑1 (MQ=255)
         gtgCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCa                                                                                        <  1:3418593/101‑1 (MQ=255)
         gtgCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCa                                                                                        <  1:3418413/101‑1 (MQ=255)
           gCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATa                                                                                      <  2:3418852/101‑1 (MQ=255)
           gCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATa                                                                                      >  1:3419083/1‑101 (MQ=255)
             cTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAg                                                                                    >  2:3419088/1‑101 (MQ=255)
             cTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAg                                                                                    >  1:3419091/1‑101 (MQ=255)
               ggATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCa                                                                                  >  2:3419093/1‑101 (MQ=255)
                gATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCaa                                                                                 <  2:3418544/101‑1 (MQ=255)
                  tCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACg                                                                               >  2:3419098/1‑101 (MQ=255)
                   cAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGa                                                                              <  1:3418394/101‑1 (MQ=255)
                   cAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGa                                                                              <  1:3418818/101‑1 (MQ=255)
                    aGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGAc                                                                             >  1:3419102/1‑101 (MQ=255)
                     ggTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACa                                                                            >  2:3419104/1‑101 (MQ=255)
                     ggTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACa                                                                            <  2:3418573/101‑1 (MQ=255)
                        tCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCa                                                                         <  1:3418626/101‑1 (MQ=255)
                         cGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCAc                                                                        <  1:3418908/101‑1 (MQ=255)
                           caggcagAACAGCAGCACGGTAGCTGCCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGc                                                                      <  2:3418590/101‑1 (MQ=255)
                            aggcagAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCa                                                                     <  1:3418424/101‑1 (MQ=255)
                            aggcagAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCa                                                                     <  1:3418455/101‑1 (MQ=255)
                            aggcagAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCa                                                                     <  2:3418301/101‑1 (MQ=255)
                            aggcagAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCa                                                                     >  2:3419109/1‑101 (MQ=255)
                             ggcagAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAg                                                                    <  2:3418452/101‑1 (MQ=255)
                             ggcagAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAg                                                                    <  1:3418612/101‑1 (MQ=255)
                             ggcagAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAg                                                                    <  2:3418509/101‑1 (MQ=255)
                                agAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAgcgc                                                                 >  1:3419112/1‑101 (MQ=255)
                                  aaCAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCct                                                               <  2:3418553/101‑1 (MQ=255)
                                  aaCAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCct                                                               <  1:3418979/101‑1 (MQ=255)
                                   aCAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCctg                                                              >  2:3419116/1‑101 (MQ=255)
                                      gcagcaCGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCctgctg                                                           >  1:3419120/1‑101 (MQ=255)
                                      gcagcaCGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCctgctg                                                           >  2:3419121/1‑101 (MQ=255)
                                          caCGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAg                                                       >  2:3419126/1‑101 (MQ=255)
                                           aCGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGt                                                      >  1:3419127/1‑101 (MQ=255)
                                            cGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTc                                                     <  2:3418367/101‑1 (MQ=255)
                                             ggTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCa                                                    <  2:3418518/101‑1 (MQ=255)
                                             ggTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCa                                                    <  2:3418737/101‑1 (MQ=255)
                                              gTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCAt                                                   <  2:3418829/101‑1 (MQ=255)
                                               tAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCAta                                                  <  2:3418537/101‑2 (MQ=255)
                                               tAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATc                                                  >  1:3419133/1‑101 (MQ=255)
                                                aGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCg                                                 >  2:3419135/1‑101 (MQ=255)
                                                  cTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGc                                               <  1:3418580/101‑1 (MQ=255)
                                                   tGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCa                                              <  2:3418477/101‑1 (MQ=255)
                                                   tGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCa                                              >  2:3419139/1‑101 (MQ=255)
                                                    gTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCaa                                             >  1:3419143/1‑101 (MQ=255)
                                                     tCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAAt                                            <  2:3418643/101‑1 (MQ=255)
                                                     tCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAAt                                            >  2:3419144/1‑101 (MQ=255)
                                                       gTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAAttt                                          <  2:3418357/101‑1 (MQ=255)
                                                         aaTAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGc                                        >  2:3419150/1‑101 (MQ=255)
                                                          aTAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCt                                       >  1:3419151/1‑101 (MQ=255)
                                                           tAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCtt                                      <  1:3418513/101‑1 (MQ=255)
                                                              gCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGaa                                   >  2:3419156/1‑101 (MQ=255)
                                                              gCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGaa                                   <  1:3418784/101‑1 (MQ=255)
                                                              gCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGaa                                   <  1:3418711/101‑1 (MQ=255)
                                                               cAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAg                                  <  1:3418828/101‑1 (MQ=255)
                                                               cAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAg                                  <  1:3418522/101‑1 (MQ=255)
                                                               cAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAg                                  >  1:3419157/1‑101 (MQ=255)
                                                               cAACCGCTGCGGAGAAGGTCGCAACCGGAATACCGCTCTGTACTGCATaa                                                                                     <  1:3418295/50‑1 (MQ=255)
                                                                aaCCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGt                                 <  2:3418428/101‑1 (MQ=255)
                                                                 aCCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTa                                <  1:3418685/101‑1 (MQ=255)
                                                                 aCCGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTa                                <  1:3418772/101‑1 (MQ=255)
                                                                  ccGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTAc                               >  2:3419162/1‑101 (MQ=255)
                                                                   cGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACg                              >  1:3419166/1‑101 (MQ=255)
                                                                   cGCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACg                              >  2:3419165/1‑101 (MQ=255)
                                                                    gCTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACgg                             <  1:3418538/101‑1 (MQ=255)
                                                                     cTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGa                            <  1:3418564/101‑1 (MQ=255)
                                                                     cTGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGa                            >  1:3419170/1‑101 (MQ=255)
                                                                      tGCGGAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAAc                                                                                >  2:3419174/1‑48 (MQ=255)
                                                                         ggAGAAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCa                        >  1:3419177/1‑101 (MQ=255)
                                                                           agaAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGc                      <  2:3418605/101‑1 (MQ=255)
                                                                           agaAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGc                      <  2:3418631/101‑1 (MQ=255)
                                                                            gaAGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCCCGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACg                              <  1:3418548/92‑1 (MQ=255)
                                                                             aaGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCaa                    >  2:3419182/1‑101 (MQ=255)
                                                                             aaGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCaa                    <  2:3418762/101‑1 (MQ=255)
                                                                             aaGGTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCaa                    <  1:3418647/101‑1 (MQ=255)
                                                                               ggTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACa                  <  2:3418905/101‑1 (MQ=255)
                                                                                gTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAg                 >  1:3419183/1‑101 (MQ=255)
                                                                                gTCGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAg                 <  1:3418636/101‑1 (MQ=255)
                                                                                  cGGAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTAc                               <  2:3418936/85‑1 (MQ=255)
                                                                                   ggAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGtt              >  1:3419187/1‑101 (MQ=255)
                                                                                    gAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTa             <  1:3418730/101‑1 (MQ=255)
                                                                                    gAACCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTa             >  2:3419191/1‑101 (MQ=255)
                                                                                     aaCCGGAATACCGCTCTGTACTGCATAAGCAACGACATc                                                                          <  1:3418675/39‑1 (MQ=255)
                                                                                     aaCCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAg            >  1:3419192/1‑101 (MQ=255)
                                                                                      aCCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGc           <  1:3418716/101‑1 (MQ=255)
                                                                                      aCCGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGc           <  2:3418664/101‑1 (MQ=255)
                                                                                        cGGAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGa         >  1:3419195/1‑101 (MQ=255)
                                                                                         ggAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGAt        >  2:3419199/1‑101 (MQ=255)
                                                                                          gAATACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATc       <  2:3418689/101‑1 (MQ=255)
                                                                                           aaTACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCt      <  2:3418403/101‑1 (MQ=255)
                                                                                           aaTACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCt      >  1:3419203/1‑101 (MQ=255)
                                                                                           aaTACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCt      <  2:3418878/101‑1 (MQ=255)
                                                                                            aTACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTa                                                        <  2:3418497/50‑1 (MQ=255)
                                                                                            aTACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCtg     <  2:3418785/101‑1 (MQ=255)
                                                                                            aTACCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCtg     >  2:3419204/1‑101 (MQ=255)
                                                                                              aCCGCTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCtgtg   <  1:3418375/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
AGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGG  >  NC_000913/2099930‑2100125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑