BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,154,657	T→C	G214G (GGT→GGC)	mdtA →	multidrug efflux pump membrane fusion protein MdtA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,154,657	0	T	C	100.0%	1123.4 / NA	271	G214G (GGT→GGC)	mdtA	multidrug efflux pump membrane fusion protein MdtA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (129/142); total (129/142)

Only 100 of 271 total aligned reads displayed.

GATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTA  >  NC_000913/2154559‑2154754

 atgaAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAAc                                                                                                <  1:3511204/101‑1 (MQ=255)

  tgaAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAAcc                                                                                               >  1:3511365/1‑101 (MQ=255)

  tgaAGCAAGCGTTACCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAAcc                                                                                               >  2:3511367/1‑101 (MQ=255)

    aagcaagcGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCaa                                                                                             >  1:3511368/1‑101 (MQ=255)

     agcaagcGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCaaa                                                                                            <  1:3510864/101‑1 (MQ=255)

      gcaagcGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAAt                                                                                           <  1:3510596/101‑1 (MQ=255)

      gcaagcGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAAt                                                                                           <  1:3510825/101‑1 (MQ=255)

       caagcGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAAtc                                                                                          >  2:3511374/1‑101 (MQ=255)

        aagcGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAAtct                                                                                         >  1:3511375/1‑101 (MQ=255)

        aagcGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAAtct                                                                                         <  1:3510815/101‑1 (MQ=255)

        aagcGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAAtct                                                                                         >  2:3511377/1‑101 (MQ=255)

         agcGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAAtctc                                                                                        <  1:3510920/101‑1 (MQ=255)

          gcGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAAtct                                                                                         <  1:3511040/99‑1 (MQ=255)

           cGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCa                                                                                      <  1:3510858/101‑1 (MQ=255)

            gTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAg                                                                                     <  2:3510886/101‑1 (MQ=255)

            gTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAg                                                                                     <  1:3510393/101‑1 (MQ=255)

            gTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAg                                                                                     >  1:3511384/1‑101 (MQ=255)

            gTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAg                                                                                     >  1:3511381/1‑101 (MQ=255)

             ttGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAgt                                                                                    <  1:3511020/101‑1 (MQ=255)

              tGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAgtg                                                                                   >  2:3511386/1‑101 (MQ=255)

              tGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAgtg                                                                                   <  1:3510833/101‑1 (MQ=255)

               gCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAgtgg                                                                                  <  2:3510966/101‑1 (MQ=255)

                ccAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAgtggt                                                                                 >  1:3511389/1‑101 (MQ=255)

                 cAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAgtgg                                                                                  >  2:3511393/1‑99 (MQ=255)

                     gcgcAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATac                                                                            >  2:3511397/1‑101 (MQ=255)

                       gcAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATacca                                                                          >  1:3511401/1‑101 (MQ=255)

                        cAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATaccac                                                                         <  2:3510827/101‑1 (MQ=255)

                         aGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATaccacc                                                                        >  2:3511405/1‑101 (MQ=255)

                         aGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATaccacc                                                                        <  1:3511005/101‑1 (MQ=255)

                         aGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATaccacc                                                                        <  2:3510931/101‑1 (MQ=255)

                         aGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATaccacc                                                                        >  1:3511404/1‑101 (MQ=255)

                            tGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCggg                                                                     <  2:3510766/101‑1 (MQ=255)

                              cAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAgggg                                                                                  >  2:3511409/1‑86 (MQ=255)

                               aaCTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTcct                                                                                      <  1:3510776/81‑2 (MQ=255)

                                aCTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCg                                                                 <  1:3510952/101‑1 (MQ=255)

                                aCTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCg                                                                 <  2:3511070/101‑1 (MQ=255)

                                 cTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCgt                                                                <  2:3510911/101‑1 (MQ=255)

                                    gACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCgtggt                                                             >  2:3511412/1‑101 (MQ=255)

                                    gACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCgtggt                                                             >  1:3511414/1‑101 (MQ=255)

                                     aCTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCgtggtg                                                            >  1:3511418/1‑101 (MQ=255)

                                     aCTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCgtggtg                                                            <  1:3510969/101‑1 (MQ=255)

                                      cTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTTGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGa                                                           <  2:3510748/101‑1 (MQ=255)

                                          aGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCAc                                                       <  2:3511031/101‑1 (MQ=255)

                                            ccGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCAccc                                                     >  1:3511422/1‑101 (MQ=255)

                                             cGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCa                                                    <  2:3510875/101‑1 (MQ=255)

                                             cGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCa                                                    >  2:3511423/1‑101 (MQ=255)

                                               gATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGa                                                  >  1:3511426/1‑101 (MQ=255)

                                                aTTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCAccc                                                     >  1:3511429/1‑97 (MQ=255)

                                                aTTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGAc                                                 <  2:3511308/101‑1 (MQ=255)

                                                aTTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGAc                                                 <  1:3510898/101‑1 (MQ=255)

                                                  tACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGc                                               >  1:3511433/1‑101 (MQ=255)

                                                   aCCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCa                                              >  2:3511435/1‑101 (MQ=255)

                                                      gCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATcc                                           <  2:3510861/101‑1 (MQ=255)

                                                        aCCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTa                                         <  2:3510786/101‑1 (MQ=255)

                                                        aCCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTa                                         >  1:3511439/1‑101 (MQ=255)

                                                        aCCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTa                                         >  2:3511440/1‑101 (MQ=255)

                                                        aCCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTa                                         <  2:3511003/101‑1 (MQ=255)

                                                         ccAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTAt                                        <  1:3511034/101‑1 (MQ=255)

                                                          cAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATc                                       <  2:3510944/101‑1 (MQ=255)

                                                             tCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGAt                                    >  1:3511445/1‑101 (MQ=255)

                                                             tCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGAt                                    <  2:3511088/101‑1 (MQ=255)

                                                               gATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGAttt                                  >  2:3511446/1‑101 (MQ=255)

                                                                aTGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGAc                                                 <  2:3511016/85‑1 (MQ=255)

                                                                aTGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTa                                 >  2:3511450/1‑101 (MQ=255)

                                                                 tGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAg                                <  2:3510918/101‑1 (MQ=255)

                                                                 tGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAg                                <  2:3510884/101‑1 (MQ=255)

                                                                 tGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAg                                <  2:3511263/101‑1 (MQ=255)

                                                                  ggTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGt                               >  1:3511454/1‑101 (MQ=255)

                                                                   gTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTc                              >  2:3511458/1‑101 (MQ=255)

                                                                   gTCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTc                              >  1:3511456/1‑101 (MQ=255)

                                                                    tCGCGTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCa                                                                                      <  1:3511081/44‑1 (MQ=255)

                                                                     cgcgTTGGTCTCAAGCAGGCTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCtt                            >  1:3511461/1‑101 (MQ=255)

                                                                      gcgTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCttt                           <  2:3511158/101‑1 (MQ=255)

                                                                      gcgTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCttt                           <  1:3510994/101‑1 (MQ=255)

                                                                      gcgTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCttt                           >  2:3511462/1‑101 (MQ=255)

                                                                        gTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCCCCCAGACGCATCCTATCGATTTAGTCTTTAc                         <  1:3511074/101‑1 (MQ=255)

                                                                        gTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTAc                         >  1:3511467/1‑101 (MQ=255)

                                                                        gTTGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTAc                         >  2:3511468/1‑101 (MQ=255)

                                                                         ttGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTAcc                        >  1:3511470/1‑101 (MQ=255)

                                                                          tGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTAccc                       <  1:3510988/101‑1 (MQ=255)

                                                                          tGGTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGCGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTAccc                       >  2:3511473/1‑101 (MQ=255)

                                                                            gTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTg                     <  2:3510731/101‑1 (MQ=255)

                                                                            gTCTCAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTg                     <  1:3510979/101‑1 (MQ=255)

                                                                             tctcAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGc                    <  2:3511059/101‑1 (MQ=255)

                                                                               tcAAGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCg                  >  2:3511476/1‑101 (MQ=255)

                                                                                 aaGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGa                >  1:3511479/1‑101 (MQ=255)

                                                                                  aGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGaa               <  2:3511141/101‑1 (MQ=255)

                                                                                  aGCAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGaa               >  2:3511482/1‑101 (MQ=255)

                                                                                    cAGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGcc                   >  1:3511484/1‑95 (MQ=255)

                                                                                     aGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGc            <  1:3511045/101‑1 (MQ=255)

                                                                                     aGGTTGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGc            <  2:3510980/101‑1 (MQ=255)

                                                                                        ttGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGat         <  2:3510811/101‑1 (MQ=255)

                                                                                         tGATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGata        >  2:3511489/1‑101 (MQ=255)

                                                                                          gATGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGatat       <  2:3511417/101‑1 (MQ=255)

                                                                                           aTGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATc      >  1:3511491/1‑101 (MQ=255)

                                                                                           aTGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATc      <  1:3511397/101‑1 (MQ=255)

                                                                                            tGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCg     >  1:3511493/1‑101 (MQ=255)

                                                                                            tGTTGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCg     <  2:3511215/101‑1 (MQ=255)

                                                                                              ttGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCt   <  2:3510893/101‑1 (MQ=255)

                                                                                               tGGCAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTa  <  1:3511106/101‑1 (MQ=255)

GATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTA  >  NC_000913/2154559‑2154754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`