Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 276,826 C→T L38L (CTG→TTG)  mmuM → homocysteine S‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913276,8260CT99.7% 1221.2 / ‑5.2 282L38L (CTG→TTG) mmuMhomocysteine S‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (157/124);  total (158/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 282 total aligned reads displayed.

ATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAG  >  NC_000913/276728‑276923
                                                                                                  |                                                                                                 
atCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTtg                                                                                                 >  1:457914/1‑101 (MQ=255)
 tCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTtgt                                                                                                <  2:457312/101‑1 (MQ=255)
   cGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTgg                                                                                              <  1:457704/101‑1 (MQ=255)
    gTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGt                                                                                             <  1:457417/101‑1 (MQ=255)
     ttACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTc                                                                                            >  2:457918/1‑101 (MQ=255)
      tACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCa                                                                                           >  1:457922/1‑101 (MQ=255)
       aCGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTc                                                                                            >  2:457928/1‑99 (MQ=255)
       aCGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAg                                                                                          >  2:457924/1‑101 (MQ=255)
       aCGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAg                                                                                          >  1:457925/1‑101 (MQ=255)
         gcgcTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGcc                                                                                        <  2:457506/101‑1 (MQ=255)
         gcgcTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGcc                                                                                        >  2:457931/1‑101 (MQ=255)
           gcTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCaa                                                                                      >  1:457936/1‑101 (MQ=255)
           gcTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCaa                                                                                      >  2:457937/1‑101 (MQ=255)
              cttcttGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGt                                                                                   >  1:457941/1‑101 (MQ=255)
              cttcttGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGt                                                                                   <  1:457463/101‑1 (MQ=255)
               ttcttGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTg                                                                                  >  1:457946/1‑101 (MQ=255)
               ttcttGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTg                                                                                  >  2:457948/1‑101 (MQ=255)
                tcttGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGc                                                                                 >  2:457953/1‑101 (MQ=255)
                  ttGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTg                                                                               >  2:457955/1‑101 (MQ=255)
                     aTAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTa                                                                            <  2:457521/101‑1 (MQ=255)
                     aTAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTa                                                                            >  1:457959/1‑101 (MQ=255)
                     aTAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTa                                                                            >  1:457957/1‑101 (MQ=255)
                      tAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAg                                                                           <  1:457438/101‑1 (MQ=255)
                       aaaCAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGa                                                                          >  2:457963/1‑101 (MQ=255)
                        aaCAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGaa                                                                         >  2:457966/1‑101 (MQ=255)
                         aCAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGaaa                                                                        <  1:457603/101‑1 (MQ=255)
                         aCAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGaaa                                                                        >  1:457967/1‑101 (MQ=255)
                           aGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAAc                                                                      <  2:457461/101‑1 (MQ=255)
                           aGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAAc                                                                      <  2:457478/101‑1 (MQ=255)
                            ggATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAAcc                                                                     >  2:457971/1‑101 (MQ=255)
                            ggATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAAcc                                                                     <  2:457403/101‑1 (MQ=255)
                              atatCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCg                                                                   >  2:457974/1‑101 (MQ=255)
                              atatCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCg                                                                   <  2:457562/101‑1 (MQ=255)
                              atatCCTGCTGCTGGATGGCGCGATGGCAACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCg                                                                   <  1:457406/101‑1 (MQ=255)
                               tatCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCgg                                                                  <  1:457668/101‑1 (MQ=255)
                                atCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGa                                                                 >  1:457980/1‑101 (MQ=255)
                                atCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGa                                                                 >  2:457981/1‑101 (MQ=255)
                                 tCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAg                                                                <  1:457577/101‑1 (MQ=255)
                                   ctgctgctgGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCt                                                              <  2:457592/101‑1 (MQ=255)
                                   ctgctgctgGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCt                                                              >  2:457986/1‑101 (MQ=255)
                                    tgctgctgGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCtt                                                             <  2:457393/101‑1 (MQ=255)
                                    tgctgctgGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCtt                                                             >  2:457989/1‑101 (MQ=255)
                                     gctgctgGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTa                                                            <  2:457537/101‑1 (MQ=255)
                                     gctgctgGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTa                                                            >  1:457990/1‑101 (MQ=255)
                                      ctgctgGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTAt                                                           <  1:457455/101‑1 (MQ=255)
                                       tgctgGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATc                                                          <  1:457326/101‑1 (MQ=255)
                                        gctgGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATcc                                                         >  1:457995/1‑101 (MQ=255)
                                        gctgGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATcc                                                         <  1:457626/101‑1 (MQ=255)
                                         ctgGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCcg                                                        >  2:457997/1‑101 (MQ=255)
                                             aTGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGaa                                                    >  2:458001/1‑101 (MQ=255)
                                              tGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAg                                                   >  1:458002/1‑101 (MQ=255)
                                               ggCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGt                                                  <  1:457493/101‑1 (MQ=255)
                                                gcgcGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTg                                                 >  2:458005/1‑101 (MQ=255)
                                                 cgcgATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGc                                                >  1:458006/1‑101 (MQ=255)
                                                 cgcgATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGc                                                <  1:457654/101‑1 (MQ=255)
                                                 cgcgATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGc                                                <  2:457627/101‑1 (MQ=255)
                                                  gcgATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCa                                               <  2:457666/101‑1 (MQ=255)
                                                  gcgATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCa                                               >  2:458009/1‑101 (MQ=255)
                                                   cgATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCAt                                              <  2:457698/101‑1 (MQ=255)
                                                    gATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATc                                             >  1:458013/1‑101 (MQ=255)
                                                     aTGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCt                                            >  1:458016/1‑101 (MQ=255)
                                                       ggCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTg                                          <  1:457373/101‑1 (MQ=255)
                                                         cGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGAt                                        >  1:458020/1‑101 (MQ=255)
                                                          gACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGAtt                                       <  2:457529/101‑1 (MQ=255)
                                                           aCGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGaaaa                                                                       >  2:458024/1‑68 (MQ=255)
                                                           aCGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATta                                      >  1:458023/1‑101 (MQ=255)
                                                            cGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATtac                                     >  1:458026/1‑101 (MQ=255)
                                                             ggAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATtact                                    <  1:457511/101‑1 (MQ=255)
                                                              gAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATtacta                                   >  2:458030/1‑101 (MQ=255)
                                                              gAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATtacta                                   <  1:457545/101‑1 (MQ=255)
                                                               aGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATtactac                                  >  1:458032/1‑101 (MQ=255)
                                                                  tGGAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACcgg                               >  2:458037/1‑101 (MQ=255)
                                                                   ggAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTAcc                                 >  1:458038/1‑98 (MQ=255)
                                                                   ggAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACcggg                              <  1:457531/101‑1 (MQ=255)
                                                                   ggAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACcggg                              <  2:457613/101‑1 (MQ=255)
                                                                    gAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACcgggc                             >  2:458041/1‑101 (MQ=255)
                                                                    gAAGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACcgggc                             <  2:457414/101‑1 (MQ=255)
                                                                     aaGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACcgggcg                            <  2:457355/101‑1 (MQ=255)
                                                                      aGCGCGAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACcgggcgg                           <  2:457581/101‑1 (MQ=255)
                                                                        cgcgAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCgggg                         >  2:458043/1‑101 (MQ=255)
                                                                          cgAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGgc                       >  2:458048/1‑101 (MQ=255)
                                                                           gAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTgg                                                                              <  2:457497/45‑1 (MQ=255)
                                                                           gAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGgcg                      >  1:458049/1‑101 (MQ=255)
                                                                           gAGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGgcg                      <  2:457550/101‑1 (MQ=255)
                                                                            aGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAg                                                                           <  2:457433/47‑1 (MQ=255)
                                                                            aGGGTGTAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGgcgc                     >  1:458053/1‑101 (MQ=255)
                                                                                tgtAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATg                 <  1:457468/101‑1 (MQ=255)
                                                                                 gtAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATgc                >  2:458058/1‑101 (MQ=255)
                                                                                 gtAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATgc                >  1:458057/1‑101 (MQ=255)
                                                                                  tAACTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATgcg               >  1:458061/1‑101 (MQ=255)
                                                                                    aCTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTCGAAACCCCGGAGCTTATcc                                                         <  2:457472/57‑1 (MQ=255)
                                                                                     cTTAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGa            <  2:457810/101‑1 (MQ=255)
                                                                                      ttAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGAt           >  2:458065/1‑101 (MQ=255)
                                                                                       tAGCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATc          <  2:457978/101‑1 (MQ=255)
                                                                                         gCCGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCAc        >  1:458068/1‑101 (MQ=255)
                                                                                          ccGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACt       <  1:457559/101‑1 (MQ=255)
                                                                                           cGACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTg      >  2:458070/1‑101 (MQ=255)
                                                                                            gACAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGc     <  1:457645/101‑1 (MQ=255)
                                                                                             aCAGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGcc    >  1:458073/1‑101 (MQ=255)
                                                                                               aGCTTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAg  <  1:457862/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
ATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAG  >  NC_000913/276728‑276923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑