BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,432,464	T→C	D160G (GAC→GGC)	folC ←	bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,432,464	0	T	C	100.0%	1142.2 / NA	275	D160G (GAC→GGC)	folC	bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (148/127); total (148/127)

Only 100 of 277 total aligned reads displayed.

GGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGT  >  NC_000913/2432366‑2432561

ggAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCg                                                                                                 >  2:3961406/1‑101 (MQ=255)

ggAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCg                                                                                                 >  2:3961404/1‑101 (MQ=255)

 gAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGa                                                                                                <  2:3961060/101‑1 (MQ=255)

 gAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGa                                                                                                <  2:3961081/101‑1 (MQ=255)

  aaGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGAc                                                                                               <  2:3961002/101‑1 (MQ=255)

  aaGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGAc                                                                                               >  2:3961410/1‑101 (MQ=255)

     aTGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAat                                                                                            >  1:3961413/1‑101 (MQ=255)

         cTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTg                                                                                        >  1:3961417/1‑101 (MQ=255)

         cTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTg                                                                                        <  1:3960904/101‑1 (MQ=255)

          tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTgg                                                                                       >  1:3961421/1‑101 (MQ=255)

           gCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGt                                                                                      >  2:3961424/1‑101 (MQ=255)

            cTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGtt                                                                                     >  1:3961425/1‑101 (MQ=255)

            cTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGtt                                                                                     <  2:3961159/101‑1 (MQ=255)

             tttCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTg                                                                                    >  1:3961430/1‑101 (MQ=255)

             tttCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTg                                                                                    >  2:3961429/1‑101 (MQ=255)

              ttCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGc                                                                                   <  1:3960997/101‑1 (MQ=255)

              ttCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGc                                                                                   <  1:3961037/101‑1 (MQ=255)

               tctcGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCg                                                                                  <  2:3960959/101‑1 (MQ=255)

               tctcGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCg                                                                                  <  1:3961181/101‑1 (MQ=255)

                ctcGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGt                                                                                 >  1:3961434/1‑101 (MQ=255)

                 tcGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTc                                                                                <  1:3960930/101‑1 (MQ=255)

                  cgcgACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTcc                                                                               >  2:3961437/1‑101 (MQ=255)

                   gcgACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCa                                                                              <  1:3961064/101‑1 (MQ=255)

                     gACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAga                                                                            >  1:3961439/1‑101 (MQ=255)

                      aCCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAgac                                                                           <  1:3961118/101‑1 (MQ=255)

                       ccAATCCTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTcc                                                                               <  2:3961024/96‑1 (MQ=255)

                       ccAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAgacg                                                                          <  2:3961143/101‑1 (MQ=255)

                        cAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAgacga                                                                         >  2:3961442/1‑101 (MQ=255)

                          aTACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGAcc                                                                       >  1:3961444/1‑101 (MQ=255)

                           tACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCg                                                                      <  1:3961026/101‑1 (MQ=255)

                            aCTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGc                                                                     <  1:3961007/101‑1 (MQ=255)

                              tttCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGccc                                                                   >  2:3961449/1‑101 (MQ=255)

                              tttCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGccc                                                                   >  1:3961448/1‑101 (MQ=255)

                               ttCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCa                                                                  <  2:3960892/101‑1 (MQ=255)

                               ttCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCa                                                                  <  2:3961285/101‑1 (MQ=255)

                               ttCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCa                                                                  <  2:3960951/101‑1 (MQ=255)

                                tCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAg                                                                 <  2:3961067/101‑1 (MQ=255)

                                 cgcgATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGc                                                                >  2:3961453/1‑101 (MQ=255)

                                  gcgATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGCCCGCCCa                                                                  <  1:3961153/98‑1 (MQ=255)

                                   cgATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGccc                                                              >  1:3961456/1‑101 (MQ=255)

                                     aTCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTa                                                            >  2:3961460/1‑101 (MQ=255)

                                      tCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCg                                                                                  >  2:3961463/1‑78 (MQ=255)

                                       cTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACt                                                          >  1:3961464/1‑101 (MQ=255)

                                         ggACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTc                                                        <  2:3961047/101‑1 (MQ=255)

                                            cccAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAg                                                     >  2:3961469/1‑101 (MQ=255)

                                              cagccagTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGaa                                                   <  2:3961108/101‑1 (MQ=255)

                                              cagccagTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGaa                                                   >  1:3961471/1‑101 (MQ=255)

                                               agccagTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAAt                                                  <  1:3960952/101‑1 (MQ=255)

                                               agccagTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAAt                                                  >  2:3961473/1‑101 (MQ=255)

                                                 ccagTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATca                                                <  1:3961169/101‑1 (MQ=255)

                                                 ccagTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATca                                                >  1:3961475/1‑101 (MQ=255)

                                                  cagTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATcac                                               <  1:3961247/101‑1 (MQ=255)

                                                    gTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATcacca                                             >  1:3961480/1‑101 (MQ=255)

                                                     tCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATcaccac                                            >  2:3961481/1‑101 (MQ=255)

                                                      cGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCTAGACGACCGCCCAGCCCTACTTCCAGAATCACCACg                                           <  1:3960729/101‑1 (MQ=255)

                                                      cGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACg                                           >  2:3961483/1‑101 (MQ=255)

                                                        gTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTc                                         <  2:3960933/101‑1 (MQ=255)

                                                        gTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTc                                         <  2:3961102/101‑1 (MQ=255)

                                                          aTGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCaa                                       <  2:3961020/101‑1 (MQ=255)

                                                          aTGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCaa                                       >  1:3961488/1‑101 (MQ=255)

                                                           tGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAg                                      <  2:3960992/101‑1 (MQ=255)

                                                            gATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGt                                     >  2:3961490/1‑101 (MQ=255)

                                                            gATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGt                                     >  1:3961493/1‑101 (MQ=255)

                                                              tCCAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTtg                                   >  2:3961497/1‑101 (MQ=255)

                                                               ccAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTtgt                                  >  1:3961498/1‑101 (MQ=255)

                                                                cAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTtgtg                                 >  1:3961503/1‑101 (MQ=255)

                                                                cAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTtgtg                                 >  2:3961502/1‑101 (MQ=255)

                                                                cAGCGCAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTtgtg                                 <  1:3961051/101‑1 (MQ=255)

                                                                    gcAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTg                             <  2:3960875/101‑1 (MQ=255)

                                                                    gcAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTg                             <  1:3961111/101‑1 (MQ=255)

                                                                    gcAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTg                             <  2:3961346/101‑1 (MQ=255)

                                                                     cAATACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTtgtg                                 >  2:3961506/1‑96 (MQ=255)

                                                                      aaTACTGGTTACTACCGCGACATCGGCGCTGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCt                           <  1:3960867/101‑1 (MQ=255)

                                                                        tACTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTg                         <  1:3961092/101‑1 (MQ=255)

                                                                         aCTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGa                        >  2:3961510/1‑101 (MQ=255)

                                                                         aCTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGa                        <  1:3961021/101‑1 (MQ=255)

                                                                         aCTGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGa                        >  1:3961511/1‑101 (MQ=255)

                                                                           tGGTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAAc                      <  1:3961083/101‑1 (MQ=255)

                                                                            ggTTACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACa                     <  1:3961375/101‑1 (MQ=255)

                                                                              ttACTACCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGc                   >  1:3961516/1‑101 (MQ=255)

                                                                               tactacCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGcc                  <  2:3961224/101‑1 (MQ=255)

                                                                               tactacCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGcc                  >  1:3961519/1‑101 (MQ=255)

                                                                                 ctacCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCcac                >  1:3961522/1‑101 (MQ=255)

                                                                                 ctacCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCcac                >  2:3961521/1‑101 (MQ=255)

                                                                                  tacCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCt                              >  2:3961526/1‑86 (MQ=255)

                                                                                  tacCGCGACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCcaca               <  2:3961114/101‑1 (MQ=255)

                                                                                     cgcgACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACg            >  2:3961529/1‑101 (MQ=255)

                                                                                      gcgACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGc           >  2:3961531/1‑101 (MQ=255)

                                                                                      gcgACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGc           <  2:3961090/101‑1 (MQ=255)

                                                                                       cgACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGcc          >  1:3961534/1‑101 (MQ=255)

                                                                                       cgACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGcc          <  1:3961225/101‑1 (MQ=255)

                                                                                       cgACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGcc          <  2:3961125/101‑1 (MQ=255)

                                                                                        gACATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCg         <  1:3960827/101‑1 (MQ=255)

                                                                                         aCATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAAc             >  1:3961537/1‑96 (MQ=255)

                                                                                          cATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCcac                >  2:3961544/1‑92 (MQ=255)

                                                                                          cATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGAc       <  2:3961251/101‑1 (MQ=255)

                                                                                          cATCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGAc       >  2:3961539/1‑101 (MQ=255)

                                                                                           aTCGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACa      >  1:3961545/1‑101 (MQ=255)

                                                                                             cGGCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGc    >  2:3961549/1‑101 (MQ=255)

                                                                                              ggCGCCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGcg   >  1:3961551/1‑101 (MQ=255)

GGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAATATTGGTTGCGTCCAGACGACCGCCCAGCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGT  >  NC_000913/2432366‑2432561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`