BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,590,698	G→A	R1035H (CGC→CAC)	acrD →	multidrug efflux pump RND permease AcrD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,590,698	0	G	A	100.0%	1310.5 / NA	321	R1035H (CGC→CAC)	acrD	multidrug efflux pump RND permease AcrD
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (165/156); total (165/156)

Only 100 of 325 total aligned reads displayed.

TGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTT  >  NC_000913/2590600‑2590795

tGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCAcc                                                                                                 >  1:4218944/1‑101 (MQ=255)

tGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCAcc                                                                                                 >  2:4218942/1‑101 (MQ=255)

  gCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCg                                                                                               >  2:4218946/1‑101 (MQ=255)

   cGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCgg                                                                                              <  2:4218553/101‑1 (MQ=255)

    gTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGa                                                                                             <  1:4218500/101‑1 (MQ=255)

     tAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGaa                                                                                            <  2:4218735/101‑1 (MQ=255)

       aTGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATa                                                                                          >  1:4218954/1‑101 (MQ=255)

       aTGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATa                                                                                          >  2:4218952/1‑101 (MQ=255)

        tGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATaa                                                                                         <  1:4218636/101‑1 (MQ=255)

           gCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCa                                                                                      >  2:4218959/1‑101 (MQ=255)

            cGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCaa                                                                                     >  2:4218962/1‑101 (MQ=255)

              ggATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATa                                                                                   <  2:4218503/101‑1 (MQ=255)

               gatgatTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATaa                                                                                  <  2:4218482/101‑1 (MQ=255)

                atgatTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATaaa                                                                                 <  2:4218424/101‑1 (MQ=255)

                atgatTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATaaa                                                                                 >  1:4218966/1‑101 (MQ=255)

                   atTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATaaaaaa                                                                              >  1:4218970/1‑101 (MQ=255)

                    tttCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAg                                                                             >  2:4218971/1‑101 (MQ=255)

                     ttCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAgg                                                                            <  2:4218447/101‑1 (MQ=255)

                      tCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGc                                                                           <  2:4218463/101‑1 (MQ=255)

                      tCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGc                                                                           <  2:4218588/101‑1 (MQ=255)

                       cGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATaa                                                                                  >  2:4218979/1‑93 (MQ=255)

                       cGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCg                                                                          >  1:4218978/1‑101 (MQ=255)

                           cACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACAt                                                                      <  1:4218603/101‑1 (MQ=255)

                           cACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACAt                                                                      >  1:4218985/1‑101 (MQ=255)

                           cACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACAt                                                                      <  2:4218634/101‑1 (MQ=255)

                           cACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACAt                                                                      <  1:4218579/101‑1 (MQ=255)

                            aCTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATg                                                                     <  1:4218377/101‑1 (MQ=255)

                            aCTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATg                                                                     >  2:4218986/1‑101 (MQ=255)

                             cTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGc                                                                    >  2:4218993/1‑101 (MQ=255)

                             cTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGc                                                                    >  1:4218990/1‑101 (MQ=255)

                                ttCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCaa                                                                 >  1:4218997/1‑101 (MQ=255)

                                ttCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCaa                                                                 >  1:4219001/1‑101 (MQ=255)

                                ttCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCaa                                                                 <  1:4218328/101‑1 (MQ=255)

                                 tCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAAt                                                                >  1:4219004/1‑101 (MQ=255)

                                  cTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAAtg                                                               >  2:4219007/1‑101 (MQ=255)

                                   tGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAAtgt                                                              <  1:4218441/101‑1 (MQ=255)

                                     gCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATgtgt                                                            >  2:4219014/1‑101 (MQ=255)

                                     gCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATgtgt                                                            >  1:4219016/1‑101 (MQ=255)

                                      cTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTc                                                           >  2:4219020/1‑101 (MQ=255)

                                       tATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCg                                                          >  1:4219025/1‑101 (MQ=255)

                                       tATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCg                                                          >  2:4219024/1‑101 (MQ=255)

                                        aTTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGc                                                         >  1:4219028/1‑101 (MQ=255)

                                         tttACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGcc                                                        >  1:4219031/1‑101 (MQ=255)

                                             cTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCtttt                                                    >  1:4219037/1‑101 (MQ=255)

                                             cTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCtttt                                                    <  1:4218521/101‑1 (MQ=255)

                                              ttCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCttttt                                                   <  2:4218723/101‑1 (MQ=255)

                                                cGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCa                                                 <  2:4218844/101‑1 (MQ=255)

                                                 gTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCaa                                                >  2:4219039/1‑101 (MQ=255)

                                                   gCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACt                                              >  1:4219042/1‑101 (MQ=255)

                                                    ccGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACtt                                             <  2:4218492/101‑1 (MQ=255)

                                                      gCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTc                                           >  2:4219047/1‑101 (MQ=255)

                                                       cTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTc                                                  <  2:4218567/93‑1 (MQ=255)

                                                       cTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTcc                                          >  1:4219050/1‑101 (MQ=255)

                                                        tGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCg                                         >  2:4219052/1‑101 (MQ=255)

                                                         gTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGa                                        <  1:4218472/101‑1 (MQ=255)

                                                          ttCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAAtgt                                                              <  2:4218387/78‑1 (MQ=255)

                                                          ttCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGAt                                       >  1:4219055/1‑101 (MQ=255)

                                                            cTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTa                                     >  1:4219060/1‑101 (MQ=255)

                                                            cTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTa                                     >  2:4219062/1‑101 (MQ=255)

                                                             tttGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTaa                                    <  1:4218401/101‑1 (MQ=255)

                                                               tgtgCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGa                                  >  1:4219067/1‑101 (MQ=255)

                                                                 tgCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAAc                                <  1:4218646/101‑1 (MQ=255)

                                                                 tgCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAAc                                >  2:4219069/1‑101 (MQ=255)

                                                                     ggTGCGCCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGc                            <  1:4218727/101‑1 (MQ=255)

                                                                        gcgcCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTc                                                           <  2:4218622/67‑1 (MQ=255)

                                                                        gcgcCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCa                         <  1:4218958/101‑1 (MQ=255)

                                                                        gcgcCGCCGCTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCa                         >  1:4219074/1‑101 (MQ=255)

                                                                          gccgccgcTTCCCGCTGAAGCCGCACCCGGAATAAGCAATaaaaa                                                                               <  2:4217869/45‑1 (MQ=255)

                                                                          gccgccgcTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTc                                                           <  1:4218495/65‑1 (MQ=255)

                                                                           ccgccgcTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTaa                                    <  2:4218369/87‑1 (MQ=255)

                                                                           ccgccgcTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGa                                  <  2:4218436/89‑1 (MQ=255)

                                                                           ccgccgcTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCg                      <  2:4218705/101‑1 (MQ=255)

                                                                            cgccgcTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCgg                     >  2:4219077/1‑101 (MQ=255)

                                                                             gccgcTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACt                                              <  2:4218664/75‑1 (MQ=255)

                                                                             gccgcTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCggg                    >  1:4219080/1‑101 (MQ=255)

                                                                               cgcTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGtt                  <  2:4218558/101‑1 (MQ=255)

                                                                               cgcTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGtt                  >  1:4219085/1‑101 (MQ=255)

                                                                               cgcTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGtt                  >  2:4219083/1‑101 (MQ=255)

                                                                                gcTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAg                        <  1:4218551/94‑1 (MQ=255)

                                                                                 cTTCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCt                <  2:4218610/101‑1 (MQ=255)

                                                                                  ttCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCtt               >  1:4219091/1‑101 (MQ=255)

                                                                                  ttCCCGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCtt               >  2:4219090/1‑101 (MQ=255)

                                                                                    cccGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCTTTTTTCAACTTTCCGATTAAGa                                  <  1:4218047/80‑1 (MQ=255)

                                                                                    cccGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTc                 <  2:4218517/97‑1 (MQ=255)

                                                                                     ccGCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCt            <  1:4218765/101‑1 (MQ=255)

                                                                                       gCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCTCCTTTTTc                                                  <  2:4218405/61‑1 (MQ=255)

                                                                                       gCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGt          <  1:4218507/101‑1 (MQ=255)

                                                                                       gCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGt          >  1:4219095/1‑101 (MQ=255)

                                                                                       gCTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGt          <  1:4218676/101‑1 (MQ=255)

                                                                                        cTGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGtt         >  2:4219098/1‑101 (MQ=255)

                                                                                         tGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGttt        <  1:4218782/101‑1 (MQ=255)

                                                                                         tGAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGttt        <  1:4218529/101‑1 (MQ=255)

                                                                                          gAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTg       <  2:4218835/101‑1 (MQ=255)

                                                                                          gAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTg       >  1:4219105/1‑101 (MQ=255)

                                                                                          gAAGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTg       >  1:4219101/1‑101 (MQ=255)

                                                                                           aaGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTaa                                    <  1:4218460/71‑1 (MQ=255)

                                                                                           aaGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGt      <  2:4218779/101‑1 (MQ=255)

                                                                                           aaGCCGCACCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGt      <  1:4218435/101‑1 (MQ=255)

                                                                                               cgcaCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACtt  <  1:4219011/101‑1 (MQ=255)

                                                                                               cgcaCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACtt  <  1:4218539/101‑1 (MQ=255)

TGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTT  >  NC_000913/2590600‑2590795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`