BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,100,491	A→G	S80P (TCC→CCC)	ansB ←	L‑asparaginase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,100,491	0	A	G	100.0%	1467.7 / NA	357	S80P (TCC→CCC)	ansB	L‑asparaginase 2
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (199/158); total (199/158)

Only 100 of 361 total aligned reads displayed.

GTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTG  >  NC_000913/3100393‑3100588

gtCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGc                                                                                                 <  1:5059207/101‑1 (MQ=255)

  cGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCg                                                                                               <  2:5059300/101‑1 (MQ=255)

  cGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCg                                                                                               >  1:5059687/1‑101 (MQ=255)

   ggTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGa                                                                                              >  2:5059689/1‑101 (MQ=255)

   ggTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGa                                                                                              <  1:5059153/101‑1 (MQ=255)

     tACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGata                                                                                            >  1:5059695/1‑101 (MQ=255)

       ccGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATAtt                                                                                          >  2:5059699/1‑101 (MQ=255)

          tGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCAc                                                                                       >  1:5059703/1‑101 (MQ=255)

            ggTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTa                                                                                     <  2:5059395/101‑1 (MQ=255)

            ggTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTa                                                                                     <  2:5058959/101‑1 (MQ=255)

              tAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTAcc                                                                                   <  2:5059058/101‑1 (MQ=255)

              tAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTAcc                                                                                   >  2:5059707/1‑101 (MQ=255)

              tAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTAcc                                                                                   <  1:5059269/101‑1 (MQ=255)

               aaTGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCt                                                                                  <  1:5059532/101‑1 (MQ=255)

                aTGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTg                                                                                 <  1:5059297/101‑1 (MQ=255)

                aTGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTg                                                                                 >  1:5059709/1‑101 (MQ=255)

                  gACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCt                                                                               >  1:5059712/1‑101 (MQ=255)

                   aCGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTc                                                                              <  2:5059200/101‑1 (MQ=255)

                     gAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGc                                                                            <  2:5059334/101‑1 (MQ=255)

                      aaGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGcc                                                                           >  1:5059718/1‑101 (MQ=255)

                       aGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCt                                                                          <  2:5059438/101‑1 (MQ=255)

                       aGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCt                                                                          <  1:5059432/101‑1 (MQ=255)

                        gCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCtt                                                                         >  2:5059722/1‑101 (MQ=255)

                        gCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCtt                                                                         >  1:5059723/1‑101 (MQ=255)

                         ccGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGatat                                                                                           <  2:5059461/82‑1 (MQ=255)

                          cGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTa                                                                       >  2:5059728/1‑101 (MQ=255)

                           gTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTaa                                                                      <  2:5059098/101‑1 (MQ=255)

                             cGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACg                                                                    <  2:5059004/101‑1 (MQ=255)

                             cGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACg                                                                    <  2:5059310/101‑1 (MQ=255)

                             cGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACg                                                                    >  2:5059733/1‑101 (MQ=255)

                             cGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACg                                                                    <  2:5059276/101‑1 (MQ=255)

                              ggTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGt                                                                   >  1:5059736/1‑101 (MQ=255)

                              ggTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGt                                                                   <  1:5059383/101‑1 (MQ=255)

                                tCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTc                                                                 >  2:5059738/1‑101 (MQ=255)

                                tCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTc                                                                 >  2:5059741/1‑101 (MQ=255)

                                   tATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCa                                                              >  1:5059749/1‑101 (MQ=255)

                                   tATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCa                                                              >  2:5059748/1‑101 (MQ=255)

                                    aTCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCaa                                                             <  2:5059145/101‑1 (MQ=255)

                                       gCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGt                                                          >  1:5059757/1‑101 (MQ=255)

                                       gCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGt                                                          >  2:5059756/1‑101 (MQ=255)

                                         aGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCt                                                        >  2:5059762/1‑101 (MQ=255)

                                           tCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCttt                                                      >  1:5059765/1‑101 (MQ=255)

                                           tCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCttt                                                      <  2:5059504/101‑1 (MQ=255)

                                             ggTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTa                                                    <  2:5059180/101‑1 (MQ=255)

                                             ggTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTa                                                    >  2:5059774/1‑101 (MQ=255)

                                             ggTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTa                                                    >  1:5059771/1‑101 (MQ=255)

                                             ggTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTa                                                    <  2:5059326/101‑1 (MQ=255)

                                              gtgtTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAg                                                   <  2:5059410/101‑1 (MQ=255)

                                                 ttAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTg                                                <  1:5058866/101‑1 (MQ=255)

                                                 ttAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTg                                                >  1:5059778/1‑101 (MQ=255)

                                                  tAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCttt                                                      <  1:5059125/94‑1 (MQ=255)

                                                  tAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGc                                               >  2:5059781/1‑101 (MQ=255)

                                                   aaTTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCg                                              >  1:5059787/1‑101 (MQ=255)

                                                   aaTTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCg                                              <  2:5059295/101‑1 (MQ=255)

                                                      ttttttCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCa                                           >  1:5059792/1‑101 (MQ=255)

                                                      ttttttCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCa                                           >  2:5059791/1‑101 (MQ=255)

                                                           tCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCa                                      >  1:5059801/1‑101 (MQ=255)

                                                            cGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCAt                                     >  2:5059802/1‑101 (MQ=255)

                                                             gCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCAtt                                    <  1:5058923/101‑1 (MQ=255)

                                                             gCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCAtt                                    >  2:5059805/1‑101 (MQ=255)

                                                              ccAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTa                                   <  2:5059263/101‑1 (MQ=255)

                                                               cAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTaa                                  >  2:5059812/1‑101 (MQ=255)

                                                               cAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTaa                                  >  1:5059811/1‑101 (MQ=255)

                                                                aGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGc                                       <  1:5059347/95‑1 (MQ=255)

                                                                aGTGTCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAAc                                 >  2:5059818/1‑101 (MQ=255)

                                                                 gtgtCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGc                                            <  1:5059336/89‑1 (MQ=255)

                                                                  tgtCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCa                               >  1:5059819/1‑101 (MQ=255)

                                                                   gtCAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAg                              >  2:5059823/1‑101 (MQ=255)

                                                                     cAGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGAt                            <  1:5059099/101‑1 (MQ=255)

                                                                      aGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGAtt                           >  2:5059831/1‑101 (MQ=255)

                                                                      aGCCAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGAtt                           >  1:5059830/1‑101 (MQ=255)

                                                                        ccAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGAtttt                         <  2:5059374/101‑1 (MQ=255)

                                                                        ccAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGAtttt                         >  1:5059835/1‑101 (MQ=255)

                                                                         cAGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTc                        >  2:5059837/1‑101 (MQ=255)

                                                                          aGACATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCt                       >  1:5059841/1‑101 (MQ=255)

                                                                            aCATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc                     >  1:5059847/1‑101 (MQ=255)

                                                                            aCATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc                     <  1:5059047/101‑1 (MQ=255)

                                                                            aCATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc                     <  1:5059224/101‑1 (MQ=255)

                                                                             cATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCg                                        <  2:5058789/81‑1 (MQ=255)

                                                                             cATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACg                    <  2:5059252/101‑1 (MQ=255)

                                                                             cATTATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACg                    >  2:5059851/1‑101 (MQ=255)

                                                                              aTTATCGTTCATGTCCTGGGGGCCGATATTCACTGCCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGc                   >  1:5059854/1‑101 (MQ=255)

                                                                               ttATCGTTCATGTCCTGGGGGCCGATATTCTCTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGcc                  >  2:5059856/1‑101 (MQ=255)

                                                                                tATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCa                 <  2:5059267/101‑1 (MQ=255)

                                                                                tATCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCa                 <  1:5059375/101‑1 (MQ=255)

                                                                                  tCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAAc               >  2:5059862/1‑101 (MQ=255)

                                                                                  tCGTTCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAAc               >  1:5059863/1‑101 (MQ=255)

                                                                                     ttCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACttt            <  2:5059233/101‑1 (MQ=255)

                                                                                     ttCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACttt            >  1:5059867/1‑101 (MQ=255)

                                                                                      tCATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTa           <  1:5059177/101‑1 (MQ=255)

                                                                                       cATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTAc          >  2:5059874/1‑101 (MQ=255)

                                                                                       cATGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTAc          >  1:5059873/1‑101 (MQ=255)

                                                                                         tGTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTAccc        >  2:5059878/1‑101 (MQ=255)

                                                                                          gTCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCa       <  1:5059360/101‑1 (MQ=255)

                                                                                           tCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTAc          >  1:5059883/1‑97 (MQ=255)

                                                                                           tCCTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCAc      <  2:5059574/101‑1 (MQ=255)

                                                                                            ccTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACt     <  1:5059031/101‑1 (MQ=255)

                                                                                            ccTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACt     <  1:5059165/101‑1 (MQ=255)

                                                                                            ccTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACt     >  1:5059886/1‑101 (MQ=255)

                                                                                             cTGGGGGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACtg    >  2:5059888/1‑101 (MQ=255)

GTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTG  >  NC_000913/3100393‑3100588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`