BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,204,663	T→C	intergenic (‑74/‑31)	folB ← / → plsY	dihydroneopterin aldolase/putative glycerol‑3‑phosphate acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,204,663	0	T	C	100.0%	1456.2 / NA	346	intergenic (‑74/‑31)	folB/plsY	dihydroneopterin aldolase/putative glycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (173/173); total (173/173)

Only 100 of 346 total aligned reads displayed.

GTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTC  >  NC_000913/3204565‑3204760

gTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTg                                                                                                 >  2:5237558/1‑101 (MQ=255)

gTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTg                                                                                                 <  2:5237060/101‑1 (MQ=255)

gTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTg                                                                                                 >  1:5237560/1‑101 (MQ=255)

  tGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGaa                                                                                               >  1:5237564/1‑101 (MQ=255)

    ctctATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAaca                                                                                             <  1:5236765/101‑1 (MQ=255)

    ctctATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAaca                                                                                             >  2:5237568/1‑101 (MQ=255)

     tctATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAacac                                                                                            >  2:5237572/1‑101 (MQ=255)

     tctATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAacac                                                                                            >  1:5237573/1‑101 (MQ=255)

      ctATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACg                                                                                           <  2:5237012/101‑1 (MQ=255)

      ctATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACg                                                                                           >  2:5237577/1‑101 (MQ=255)

       tataAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGa                                                                                          <  2:5237172/101‑1 (MQ=255)

       tataAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGa                                                                                          >  1:5237579/1‑101 (MQ=255)

         taAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAAt                                                                                        <  2:5237115/101‑1 (MQ=255)

         taAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAAt                                                                                        <  1:5236936/101‑1 (MQ=255)

         taAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAAt                                                                                        <  1:5236984/101‑1 (MQ=255)

          aaaTACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAAtt                                                                                       >  2:5237585/1‑101 (MQ=255)

          aaaTACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAAtt                                                                                       <  1:5237221/101‑1 (MQ=255)

           aaTACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAAttt                                                                                      <  2:5237121/101‑1 (MQ=255)

           aaTACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAAttt                                                                                      <  2:5236863/101‑1 (MQ=255)

           aaTACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAAttt                                                                                      >  1:5237587/1‑101 (MQ=255)

            aTACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAAtttt                                                                                     <  2:5237090/101‑1 (MQ=255)

            aTACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAAtttt                                                                                     <  2:5237149/101‑1 (MQ=255)

             tACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTc                                                                                    <  2:5236971/101‑1 (MQ=255)

             tACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTc                                                                                    >  2:5237590/1‑101 (MQ=255)

              aCAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCa                                                                                   <  2:5237134/101‑1 (MQ=255)

                aaTATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCaaa                                                                                 >  1:5237594/1‑101 (MQ=255)

                aaTATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCaaa                                                                                 <  2:5237104/101‑1 (MQ=255)

                 atatCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCaaaa                                                                                <  2:5237043/101‑1 (MQ=255)

                    tCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACgg                                                                             <  1:5237187/101‑1 (MQ=255)

                     ccATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGa                                                                            >  1:5237598/1‑101 (MQ=255)

                     ccATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGa                                                                            <  2:5237198/101‑1 (MQ=255)

                     ccATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGa                                                                            >  2:5237600/1‑101 (MQ=255)

                      cATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGaa                                                                           <  1:5237113/101‑1 (MQ=255)

                      cATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGaa                                                                           >  2:5237603/1‑101 (MQ=255)

                           cGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCt                                                                      <  2:5236939/101‑1 (MQ=255)

                           cGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCt                                                                      >  2:5237610/1‑101 (MQ=255)

                            gTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCtt                                                                     <  1:5236995/101‑1 (MQ=255)

                            gTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCtt                                                                     <  2:5236897/101‑1 (MQ=255)

                            gTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCtt                                                                     <  1:5237091/101‑1 (MQ=255)

                            gTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCtt                                                                     >  1:5237611/1‑101 (MQ=255)

                              cctgcctgCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTAt                                                                   >  2:5237615/1‑101 (MQ=255)

                              cctgcctgCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTAt                                                                   <  1:5237053/101‑1 (MQ=255)

                               ctgcctgcTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGa                                                                            <  2:5236987/91‑1 (MQ=255)

                               ctgcctgcTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATg                                                                  <  1:5237142/101‑1 (MQ=255)

                               ctgcctgcTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATg                                                                  >  1:5237617/1‑101 (MQ=255)

                                   ctgcTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTg                                                              >  2:5237622/1‑101 (MQ=255)

                                    tgcTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGc                                                             <  2:5236727/101‑1 (MQ=255)

                                    tgcTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGc                                                             <  1:5237171/101‑1 (MQ=255)

                                     gcTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCa                                                            >  2:5237625/1‑101 (MQ=255)

                                      cTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGt                                                               >  2:5237629/1‑97 (MQ=255)

                                       tttttGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAAt                                                          <  1:5237303/101‑1 (MQ=255)

                                        ttttGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATc                                                         <  2:5237202/101‑1 (MQ=255)

                                        ttttGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATc                                                         <  2:5237243/101‑1 (MQ=255)

                                        ttttGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATc                                                         >  1:5237633/1‑101 (MQ=255)

                                          ttGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATCATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATcgc                                                       <  1:5237132/101‑1 (MQ=255)

                                           tGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATcgcg                                                      >  1:5237636/1‑101 (MQ=255)

                                             gCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGcc                                                    >  2:5237639/1‑101 (MQ=255)

                                             gCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGcc                                                    <  1:5237033/101‑1 (MQ=255)

                                              cTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCt                                                   <  1:5237192/101‑1 (MQ=255)

                                               tAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTg                                                  >  2:5237645/1‑101 (MQ=255)

                                                aaCCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTgg                                                 >  1:5237648/1‑101 (MQ=255)

                                                   cGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAAt                                              >  1:5237652/1‑101 (MQ=255)

                                                   cGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAAt                                              <  1:5237153/101‑1 (MQ=255)

                                                    ggATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGa                                                >  2:5237655/1‑98 (MQ=255)

                                                         ccACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCt                                        <  1:5236859/101‑1 (MQ=255)

                                                         ccACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCt                                        >  1:5237661/1‑101 (MQ=255)

                                                           aCTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTc                                       >  2:5237664/1‑100 (MQ=255)

                                                             ttCCGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATc                                    >  1:5237666/1‑101 (MQ=255)

                                                               ccGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATcgc                                  >  1:5237671/1‑101 (MQ=255)

                                                                cGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATcgcg                                 <  2:5237281/101‑1 (MQ=255)

                                                                cGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATcgcg                                 <  1:5237077/101‑1 (MQ=255)

                                                                cGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATcgcg                                 <  2:5237385/101‑1 (MQ=255)

                                                                cGGCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATcgcg                                 >  2:5237672/1‑101 (MQ=255)

                                                                 ggCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGt                                <  1:5237253/101‑1 (MQ=255)

                                                                 ggCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGt                                <  2:5237251/101‑1 (MQ=255)

                                                                  gCGAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTa                               >  1:5237677/1‑101 (MQ=255)

                                                                    gAAATGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTAcc                             >  2:5237681/1‑101 (MQ=255)

                                                                       aTGTGCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACctct                          <  2:5237225/101‑1 (MQ=255)

                                                                        tgtgCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTg                         >  1:5237684/1‑101 (MQ=255)

                                                                        tgtgCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTg                         >  2:5237685/1‑101 (MQ=255)

                                                                         gtgCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGc                        <  2:5237417/101‑1 (MQ=255)

                                                                          tgCGTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCg                       <  2:5237312/101‑1 (MQ=255)

                                                                             gTATTATCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCt                    <  1:5237213/101‑1 (MQ=255)

                                                                              tattatCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTc                   >  1:5237692/1‑101 (MQ=255)

                                                                               attatCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTcc                  <  1:5237277/101‑1 (MQ=255)

                                                                               attatCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTcc                  <  2:5237649/101‑1 (MQ=255)

                                                                                ttatCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCa                 >  2:5237693/1‑101 (MQ=255)

                                                                                 tatCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCAt                >  2:5237698/1‑101 (MQ=255)

                                                                                 tatCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCAt                <  2:5237217/101‑1 (MQ=255)

                                                                                  atCCACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCAtt               >  1:5237699/1‑101 (MQ=255)

                                                                                    ccACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTc             <  2:5237472/101‑1 (MQ=255)

                                                                                    ccACAGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTc             >  2:5237704/1‑101 (MQ=255)

                                                                                       caGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAg          >  1:5237710/1‑101 (MQ=255)

                                                                                       caGATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAg          >  2:5237709/1‑101 (MQ=255)

                                                                                         gATTCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTg        >  1:5237714/1‑101 (MQ=255)

                                                                                            tCATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCa     >  2:5237720/1‑101 (MQ=255)

                                                                                             cATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCAt    <  1:5237321/101‑1 (MQ=255)

                                                                                             cATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCAt    >  1:5237725/1‑101 (MQ=255)

                                                                                             cATCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCAt    >  2:5237723/1‑101 (MQ=255)

                                                                                               tCGCTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTc  >  1:5237730/1‑101 (MQ=255)

GTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTC  >  NC_000913/3204565‑3204760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`