BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	554,040	A→G	V133A (GTA→GCA)	ppiB ←	peptidyl‑prolyl cis‑trans isomerase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	554,040	0	A	G	100.0%	1372.2 / NA	330	V133A (GTA→GCA)	ppiB	peptidyl‑prolyl cis‑trans isomerase B
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (178/152); total (178/152)

Only 100 of 330 total aligned reads displayed.

ATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTT  >  NC_000913/553942‑554137

aTTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGcc                                                                                                 <  2:912267/101‑1 (MQ=255)

 ttACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCa                                                                                                <  2:911849/101‑1 (MQ=255)

 ttACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCa                                                                                                >  2:912441/1‑101 (MQ=255)

  tACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCAc                                                                                               >  1:912443/1‑101 (MQ=255)

   aCTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACg                                                                                              <  2:912106/101‑1 (MQ=255)

    cTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACg                                                                                              <  2:911925/100‑1 (MQ=255)

    cTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGt                                                                                             >  1:912446/1‑101 (MQ=255)

    cTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGt                                                                                             <  1:911969/101‑1 (MQ=255)

       gCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCa                                                                                          >  1:912454/1‑101 (MQ=255)

       gCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTT‑GCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCAt                                                                                         >  2:912450/1‑101 (MQ=255)

         tAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATg                                                                                        <  1:911840/101‑1 (MQ=255)

           aCGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGcc                                                                                      >  1:912459/1‑101 (MQ=255)

           aCGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGcc                                                                                      <  1:911924/101‑1 (MQ=255)

             ggTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGt                                                                                    <  2:911882/101‑1 (MQ=255)

             ggTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGt                                                                                    >  1:912463/1‑101 (MQ=255)

             ggTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGt                                                                                    <  1:912017/101‑1 (MQ=255)

              gTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTc                                                                                   <  1:911894/101‑1 (MQ=255)

               tCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCa                                                                                  >  2:912466/1‑101 (MQ=255)

                cACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCaa                                                                                 >  1:912469/1‑101 (MQ=255)

                 aCGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAAc                                                                                <  2:912316/101‑1 (MQ=255)

                   gCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCa                                                                              >  2:912473/1‑101 (MQ=255)

                   gCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCa                                                                              >  1:912475/1‑101 (MQ=255)

                    cTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCAc                                                                             >  1:912478/1‑101 (MQ=255)

                       tCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTc                                                                          <  2:912059/101‑1 (MQ=255)

                        cAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCa                                                                         >  1:912489/1‑101 (MQ=255)

                        cAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCa                                                                         <  1:912103/101‑1 (MQ=255)

                        cAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCCGCCACGTCCATGCCGTCAACCACTTCa                                                                         >  2:912485/1‑101 (MQ=255)

                        cAATGATAACGTCTTCTTTTGGCACGTCCTGGGGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCa                                                                         >  1:912484/1‑101 (MQ=255)

                         aaTGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAg                                                                        <  1:912095/101‑1 (MQ=255)

                            gATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCaa                                                                     >  1:912493/1‑101 (MQ=255)

                            gATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCaa                                                                     <  2:911960/101‑1 (MQ=255)

                              tAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACAGGTTGCAACACCTTTGATTTTGTCTGCTACGTCCATGCCGTCAACCACTTCAGCaa                                                                     >  1:912497/1‑99 (MQ=255)

                               aaCGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAAcc                                                                               >  2:912500/1‑88 (MQ=255)

                                aCGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAacac                                                                 <  2:912165/101‑1 (MQ=255)

                                 cGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACg                                                                <  2:911980/101‑1 (MQ=255)

                                 cGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACg                                                                <  2:912053/101‑1 (MQ=255)

                                  gTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGc                                                               >  1:912505/1‑101 (MQ=255)

                                  gTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGc                                                               >  2:912504/1‑101 (MQ=255)

                                   tcttctTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCa                                                              <  2:912120/101‑1 (MQ=255)

                                      tcttTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTa                                                           >  2:912510/1‑101 (MQ=255)

                                      tcttTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTa                                                           <  2:912147/101‑1 (MQ=255)

                                       cttTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAg                                                          <  2:912127/101‑1 (MQ=255)

                                       cttTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAg                                                          >  1:912512/1‑101 (MQ=255)

                                        ttttGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGc                                                         <  2:912359/101‑1 (MQ=255)

                                        ttttGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGc                                                         >  1:912516/1‑101 (MQ=255)

                                         tttGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGcc                                                        >  1:912520/1‑101 (MQ=255)

                                           tGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGccc                                                       >  2:912523/1‑100 (MQ=255)

                                            ggCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCa                                                     <  2:912014/101‑1 (MQ=255)

                                            ggCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCa                                                     <  2:912001/101‑1 (MQ=255)

                                             gCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCaa                                                    >  1:912525/1‑101 (MQ=255)

                                                 gTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCtt                                                <  1:911999/101‑1 (MQ=255)

                                                  tCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCa                                                              >  2:912531/1‑86 (MQ=255)

                                                  tCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTg                                               >  1:912530/1‑101 (MQ=255)

                                                     tGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAg                                            >  1:912537/1‑101 (MQ=255)

                                                      ggTGCATTCCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAgg                                           >  2:912538/1‑101 (MQ=255)

                                                      ggTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAgg                                           <  1:911946/101‑1 (MQ=255)

                                                        tGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCt                                         <  2:912458/101‑1 (MQ=255)

                                                          cATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCttt                                       >  1:912542/1‑101 (MQ=255)

                                                           aTACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTc                                      <  1:911994/101‑1 (MQ=255)

                                                           aTACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTc                                      >  2:912547/1‑101 (MQ=255)

                                                           aTACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTc                                      >  1:912548/1‑101 (MQ=255)

                                                            tACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCg                                     <  2:911951/101‑1 (MQ=255)

                                                            tACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCg                                     >  1:912551/1‑101 (MQ=255)

                                                               cGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCa                                  >  2:912556/1‑101 (MQ=255)

                                                               cGCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCa                                  <  2:912071/101‑1 (MQ=255)

                                                                gCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCag                                 >  2:912561/1‑101 (MQ=255)

                                                                gCTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCag                                 >  1:912560/1‑101 (MQ=255)

                                                                 cTACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCaga                                <  1:912082/101‑1 (MQ=255)

                                                                  tACGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTg                                               <  1:911941/85‑1 (MQ=255)

                                                                   aCGACCGGTTGCAACACCTTTGATTTTGTCTGCCAc                                                                                               >  1:912566/1‑36 (MQ=255)

                                                                   aCGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAgaga                              <  2:911915/101‑1 (MQ=255)

                                                                    cGACCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAgaga                              >  2:912567/1‑100 (MQ=255)

                                                                      aCCGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGt                           >  1:912571/1‑101 (MQ=255)

                                                                       ccGGTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGtt                          <  1:912184/101‑1 (MQ=255)

                                                                         ggTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCa                        >  1:912578/1‑101 (MQ=255)

                                                                         ggTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCa                        >  2:912576/1‑101 (MQ=255)

                                                                          gTTGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAg                       <  1:912204/101‑1 (MQ=255)

                                                                           ttGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAgg                      <  2:912090/101‑1 (MQ=255)

                                                                            tGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAg                                                          <  1:911610/64‑1 (MQ=255)

                                                                            tGCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGa                     <  1:912130/101‑1 (MQ=255)

                                                                             gCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTa                                                           >  1:912583/1‑62 (MQ=255)

                                                                             gCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGaa                    <  1:912062/101‑1 (MQ=255)

                                                                             gCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGaa                    <  2:912226/101‑1 (MQ=255)

                                                                             gCAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGaa                    <  1:911874/101‑1 (MQ=255)

                                                                              cAACACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAg                   >  2:912584/1‑101 (MQ=255)

                                                                               aaCACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGt                  >  1:912587/1‑101 (MQ=255)

                                                                               aaCACCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGt                  <  1:912416/101‑1 (MQ=255)

                                                                                acacCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGa                     >  2:912593/1‑97 (MQ=255)

                                                                                  acCTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGt               >  2:912597/1‑101 (MQ=255)

                                                                                   ccTTTGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGtt              >  1:912599/1‑101 (MQ=255)

                                                                                     tttGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTAt            <  2:912178/101‑1 (MQ=255)

                                                                                     tttGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTAt            >  2:912603/1‑101 (MQ=255)

                                                                                      ttGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATc           <  2:912027/101‑1 (MQ=255)

                                                                                       tGATTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCa          >  1:912604/1‑101 (MQ=255)

                                                                                         aTTTTGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAAc        >  1:912608/1‑101 (MQ=255)

                                                                                          ttttGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAAcc       <  1:912038/101‑1 (MQ=255)

                                                                                            ttGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCAc     <  1:911984/101‑1 (MQ=255)

                                                                                            ttGTCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCAc     >  2:912612/1‑101 (MQ=255)

                                                                                               tCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGtt  >  1:912616/1‑101 (MQ=255)

                                                                                               tCTGCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGtt  <  1:912296/101‑1 (MQ=255)

ATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTT  >  NC_000913/553942‑554137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`