Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,083,355 A→G V823A (GTC→GCC)  fdoG ← formate dehydrogenase O subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,083,3550AG83.7% 727.8 / 120.9 264V823A (GTC→GCC) fdoGformate dehydrogenase O subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (24/19);  new base G (116/105);  total (140/124)
Fisher's exact test for biased strand distribution p-value = 7.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

TTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGT  >  NC_000913/4083257‑4083454
                                                                                                  |                                                                                                   
tttCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGcg                                                                                                   >  1:6728735/1‑101 (MQ=255)
  tCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGcgcc                                                                                                 >  1:6728739/1‑101 (MQ=255)
  tCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGcgcc                                                                                                 >  2:6728738/1‑101 (MQ=255)
    aaaCGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGcgccgc                                                                                               >  1:6728742/1‑101 (MQ=255)
     aaCGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCt                                                                                              <  2:6727969/101‑1 (MQ=255)
      aCGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTa                                                                                             <  2:6728446/101‑1 (MQ=255)
       cGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCt                                                                                              <  1:6728512/99‑1 (MQ=255)
       cGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTAc                                                                                            >  2:6728746/1‑101 (MQ=255)
           tCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCgg                                                                                        >  1:6728750/1‑101 (MQ=255)
             gTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAg                                                                                      <  2:6728333/101‑1 (MQ=255)
             gTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAg                                                                                      >  1:6728753/1‑101 (MQ=255)
             gTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAg                                                                                      >  2:6728754/1‑101 (MQ=255)
              tAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAgg                                                                                     <  2:6728383/101‑1 (MQ=255)
              tAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAgg                                                                                     <  2:6728418/101‑1 (MQ=255)
               aGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGgc                                                                                    <  2:6728425/101‑1 (MQ=255)
                   ttCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGgcgctg                                                                                >  1:6728759/1‑101 (MQ=255)
                    tCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGgcgctgc                                                                               >  2:6728761/1‑101 (MQ=255)
                    tCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGgcgctgc                                                                               <  2:6728278/101‑1 (MQ=255)
                     ccGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGgcgctgcg                                                                              >  1:6728765/1‑101 (MQ=255)
                     ccGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGgcgctgcg                                                                              >  1:6728763/1‑101 (MQ=255)
                         gAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGt                                                                          >  2:6728769/1‑101 (MQ=255)
                          aaCGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTa                                                                         >  1:6728771/1‑101 (MQ=255)
                            cGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGcgcc                                                                                                 >  1:6728776/1‑75 (MQ=255)
                            cGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGt                                                                       <  2:6728372/101‑1 (MQ=255)
                            cGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGt                                                                       >  2:6728775/1‑101 (MQ=255)
                             ggACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTc                                                                      >  1:6728779/1‑101 (MQ=255)
                             ggACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTc                                                                      <  1:6728388/101‑1 (MQ=255)
                             ggACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTc                                                                      <  1:6728346/101‑1 (MQ=255)
                              gACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGgcg                                                                                   >  2:6728781/1‑87 (MQ=255)
                               aCCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCg                                                                    <  1:6728422/101‑1 (MQ=255)
                               aCCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCg                                                                    >  1:6728783/1‑101 (MQ=255)
                                ccTTCCGCCATCTTATTGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCgg                                                                   <  1:6728129/101‑1 (MQ=255)
                                 cTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGa                                                                  >  1:6728786/1‑101 (MQ=255)
                                  ttCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGaa                                                                 >  2:6728789/1‑101 (MQ=255)
                                    ccGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAata                                                               <  1:6728459/101‑1 (MQ=255)
                                     cGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAatat                                                              <  2:6728498/101‑1 (MQ=255)
                                     cGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAatat                                                              >  1:6728793/1‑101 (MQ=255)
                                      gCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATc                                                             <  1:6727974/101‑1 (MQ=255)
                                      gCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATc                                                             <  2:6728223/101‑1 (MQ=255)
                                      gCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATc                                                             <  1:6728270/101‑1 (MQ=255)
                                      gCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATc                                                             <  1:6728107/101‑1 (MQ=255)
                                       ccATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATcc                                                            >  2:6728795/1‑101 (MQ=255)
                                        cATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATccc                                                           >  2:6728797/1‑101 (MQ=255)
                                          tCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAg                                                         <  2:6728310/101‑1 (MQ=255)
                                          tCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAg                                                         <  2:6728409/101‑1 (MQ=255)
                                           cTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGc                                                        <  2:6728259/101‑1 (MQ=255)
                                            ttATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGcc                                                       >  1:6728800/1‑101 (MQ=255)
                                            ttATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGcc                                                       <  1:6728453/101‑1 (MQ=255)
                                             tATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCg                                                      <  2:6728181/101‑1 (MQ=255)
                                              aTCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCgg                                                     >  2:6728804/1‑101 (MQ=255)
                                              aTCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCgg                                                     <  2:6728204/101‑1 (MQ=255)
                                                cGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTc                                                   <  2:6728436/101‑1 (MQ=255)
                                                 gAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTcc                                                  >  1:6728807/1‑101 (MQ=255)
                                                 gAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTcc                                                  <  2:6728405/101‑1 (MQ=255)
                                                  aGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCa                                                 <  2:6728320/101‑1 (MQ=255)
                                                   gggCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCAc                                                >  1:6728809/1‑101 (MQ=255)
                                                     gCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACtt                                              <  1:6728339/101‑1 (MQ=255)
                                                     gCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACtt                                              >  2:6728810/1‑101 (MQ=255)
                                                      cAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTa                                             <  2:6728599/101‑1 (MQ=255)
                                                       aaaCAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAg                                            >  2:6728813/1‑101 (MQ=255)
                                                        aaCAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGt                                           >  2:6728817/1‑101 (MQ=255)
                                                        aaCAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCg                                                                    >  1:6728818/1‑76 (MQ=255)
                                                         aCAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTg                                          <  2:6728176/101‑1 (MQ=255)
                                                          cAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGc                                         <  1:6728221/101‑1 (MQ=255)
                                                            gACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCg                                       >  2:6728821/1‑101 (MQ=255)
                                                            gACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCg                                       <  1:6728356/101‑1 (MQ=255)
                                                             aCGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGt                                      >  2:6728824/1‑101 (MQ=255)
                                                              cGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTc                                     >  1:6728827/1‑101 (MQ=255)
                                                               gCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTcc                                    <  2:6728364/101‑1 (MQ=255)
                                                               gCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTcc                                    <  1:6728254/101‑1 (MQ=255)
                                                                ccccATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTccc                                   >  2:6728829/1‑101 (MQ=255)
                                                                 cccATGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCa                                  <  2:6728291/101‑1 (MQ=255)
                                                                   cATGCCTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCAtt                                >  1:6728831/1‑101 (MQ=255)
                                                                    aTGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCAttt                               <  1:6728307/101‑1 (MQ=255)
                                                                    aTGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCAttt                               >  1:6728834/1‑101 (MQ=255)
                                                                    aTGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCAttt                               <  2:6728575/101‑1 (MQ=255)
                                                                     tGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTc                              <  1:6728485/101‑1 (MQ=255)
                                                                     tGCCTTCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTc                              >  1:6728836/1‑101 (MQ=255)
                                                                      gCCTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCa                             >  1:6728839/1‑101 (MQ=255)
                                                                      gCCTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCa                             <  2:6728083/101‑1 (MQ=255)
                                                                       ccTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAg                            >  2:6728842/1‑101 (MQ=255)
                                                                         ttCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGgcgc                                                                                  >  2:6728847/1‑45 (MQ=255)
                                                                         ttCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGc                                                        >  1:6728846/1‑71 (MQ=255)
                                                                          tCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCaa                         <  2:6728450/101‑1 (MQ=255)
                                                                          tCCTGCTGCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCaa                         >  1:6728850/1‑101 (MQ=255)
                                                                            ctgctgCATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACt                       >  1:6728854/1‑101 (MQ=255)
                                                                             tgctgcATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTg                      >  2:6728856/1‑101 (MQ=255)
                                                                              gctgcATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGa                     >  1:6728858/1‑101 (MQ=255)
                                                                              gctgcATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGa                     <  1:6728175/101‑1 (MQ=255)
                                                                               ctgcATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGAc                    >  1:6728860/1‑101 (MQ=255)
                                                                               ctgcATGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGAc                    <  1:6728401/101‑1 (MQ=255)
                                                                                   aTGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCtt                <  1:6728502/101‑1 (MQ=255)
                                                                                    tGATAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTc               <  1:6728243/101‑1 (MQ=255)
                                                                                      aTAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCgg             >  2:6728865/1‑101 (MQ=255)
                                                                                      aTAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCgg             <  1:6728300/101‑1 (MQ=255)
                                                                                       tAAACGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGa            >  2:6728867/1‑101 (MQ=255)
                                                                                          aCGGCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATcc         >  1:6728868/1‑101 (MQ=255)
                                                                                            ggCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCa       >  1:6728872/1‑101 (MQ=255)
                                                                                             gCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCAc      <  1:6728381/101‑1 (MQ=255)
                                                                                             gCCCGGCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCAc      >  2:6728874/1‑101 (MQ=255)
                                                                                                  |                                                                                                   
TTTCAAACGGCTCGTAGTGTTCCGGGAACGGACCTTCCGCCATCTTATCGAGGGCAAACAGACGCCCCATGCCTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGT  >  NC_000913/4083257‑4083454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑