BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	83,387	G→A	P48L (CCC→CTC)	leuA ←	2‑isopropylmalate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	83,387	0	G	A	100.0%	1529.0 / NA	359	P48L (CCC→CTC)	leuA	2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (178/181); total (178/181)

Only 100 of 362 total aligned reads displayed.

TATCTTTTTCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTC  >  NC_000913/83289‑83484

tatCTTTTTCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACgagg                                                                                                 >  1:166504/1‑101 (MQ=255)

 atCTTTTTCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGa                                                                                                <  2:166193/101‑1 (MQ=255)

   cTTTTTCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGaaa                                                                                              >  2:166509/1‑101 (MQ=255)

   cTTTTTCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGaaa                                                                                              <  1:165961/101‑1 (MQ=255)

    tttttCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAAc                                                                                             <  2:166292/101‑1 (MQ=255)

     ttttCCACGCAGCGAGCTAACGCACATACGGGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAAcc                                                                                            <  1:165990/101‑1 (MQ=255)

     ttttCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAAcc                                                                                            >  1:166512/1‑101 (MQ=255)

      tttCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCg                                                                                           >  2:166514/1‑101 (MQ=255)

        tCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGAc                                                                                         >  1:166518/1‑101 (MQ=255)

           aCGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTc                                                                                      >  1:166522/1‑101 (MQ=255)

            cGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTcc                                                                                     <  1:166162/101‑1 (MQ=255)

            cGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTcc                                                                                     <  1:165658/101‑1 (MQ=255)

             gCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCa                                                                                    >  1:166526/1‑101 (MQ=255)

               aGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATc                                                                                  >  2:166531/1‑101 (MQ=255)

                gCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCa                                                                                 >  2:166534/1‑101 (MQ=255)

                 cGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCAc                                                                                <  1:166232/101‑1 (MQ=255)

                  gAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACg                                                                               >  1:166538/1‑101 (MQ=255)

                     cTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCa                                                                            <  2:165963/101‑1 (MQ=255)

                      tAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCaa                                                                           >  2:166543/1‑101 (MQ=255)

                        aCGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAaca                                                                         >  1:166548/1‑101 (MQ=255)

                         cGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAacac                                                                        >  2:166551/1‑101 (MQ=255)

                           cacaTACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACAccc                                                                      >  1:166554/1‑101 (MQ=255)

                           cacaTACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACAccc                                                                      <  1:166055/101‑1 (MQ=255)

                           cacaTACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACAccc                                                                      <  1:166084/101‑1 (MQ=255)

                             caTACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCAt                                                                    >  1:166559/1‑101 (MQ=255)

                              aTACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATa                                                                   >  2:166562/1‑101 (MQ=255)

                               tACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATAc                                                                  <  2:165804/101‑1 (MQ=255)

                               tACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATAc                                                                  >  1:166566/1‑101 (MQ=255)

                                aCGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCaa                                                                           <  1:165851/91‑1 (MQ=255)

                                 cgcgGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGc                                                                >  2:166571/1‑101 (MQ=255)

                                 cgcgGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGc                                                                >  1:166572/1‑101 (MQ=255)

                                   cgGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTc                                                              >  2:166577/1‑101 (MQ=255)

                                      cTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAg                                                           <  1:166005/101‑1 (MQ=255)

                                      cTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAg                                                           >  2:166583/1‑101 (MQ=255)

                                      cTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAg                                                           >  1:166584/1‑101 (MQ=255)

                                       tGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAgg                                                          >  1:166589/1‑101 (MQ=255)

                                         tttttAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGc                                                        <  2:165842/101‑1 (MQ=255)

                                         tttttAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGc                                                        <  1:165940/101‑1 (MQ=255)

                                          ttttAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGcc                                                       >  2:166596/1‑101 (MQ=255)

                                           tttAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCa                                                      >  1:166600/1‑101 (MQ=255)

                                           tttAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCa                                                      >  2:166599/1‑101 (MQ=255)

                                             tAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGcc                                                       >  2:166607/1‑98 (MQ=255)

                                              aaCCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAgcg                                                   <  2:166031/101‑1 (MQ=255)

                                              aaCCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAgcg                                                   <  2:165949/101‑1 (MQ=255)

                                               aCCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAgcgc                                                  >  1:166608/1‑101 (MQ=255)

                                                 cTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCaa                                                <  2:165896/101‑1 (MQ=255)

                                                 cTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCaa                                                <  2:165931/101‑1 (MQ=255)

                                                 cTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCaa                                                >  1:166614/1‑101 (MQ=255)

                                                  tGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAAt                                               >  2:166615/1‑101 (MQ=255)

                                                   ggcgggcgATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAAtt                                              <  1:166194/101‑1 (MQ=255)

                                                    gcgggcgATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAAttt                                             <  1:165892/101‑1 (MQ=255)

                                                     cgggcgATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTg                                            >  2:166620/1‑101 (MQ=255)

                                                     cgggcgATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTg                                            <  1:166017/101‑1 (MQ=255)

                                                      gggcgATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAgcg                                                   <  2:166165/93‑1 (MQ=255)

                                                      gggcgATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGc                                           >  1:166622/1‑101 (MQ=255)

                                                         cgATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGt                                        >  2:166630/1‑101 (MQ=255)

                                                         cgATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGt                                        >  1:166628/1‑101 (MQ=255)

                                                          gATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGc                                                                <  2:166002/76‑1 (MQ=255)

                                                          gATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGtt                                       <  1:166387/101‑1 (MQ=255)

                                                          gATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCAGACGCTCAAg                                                           >  2:166635/1‑81 (MQ=255)

                                                            tGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGtttt                                     >  2:166639/1‑101 (MQ=255)

                                                            tGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGtttt                                     <  2:165876/101‑1 (MQ=255)

                                                             ggTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGttttt                                    <  1:165811/101‑1 (MQ=255)

                                                             ggTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGttttt                                    <  2:166205/101‑1 (MQ=255)

                                                               tttGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttct                                  <  2:166066/101‑1 (MQ=255)

                                                               tttGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttct                                  >  1:166642/1‑101 (MQ=255)

                                                                 tGCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttcttt                                <  1:166047/101‑1 (MQ=255)

                                                                  gCACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttctttc                               >  2:166645/1‑101 (MQ=255)

                                                                   cACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTca                              <  2:165989/101‑1 (MQ=255)

                                                                   cACCGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTca                              <  2:166423/101‑1 (MQ=255)

                                                                     ccGATTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTcaca                            >  1:166650/1‑101 (MQ=255)

                                                                        aTTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTc                         <  2:165977/101‑1 (MQ=255)

                                                                        aTTCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTc                         >  2:166653/1‑101 (MQ=255)

                                                                         ttCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACCCCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCa                        <  2:166058/101‑1 (MQ=255)

                                                                         ttCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCa                        <  2:166044/101‑1 (MQ=255)

                                                                          tCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCaa                       <  2:166022/101‑1 (MQ=255)

                                                                          tCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCaa                       <  1:166220/101‑1 (MQ=255)

                                                                          tCAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCa                        <  2:166121/100‑1 (MQ=255)

                                                                           cAAAATCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAg                      >  1:166654/1‑101 (MQ=255)

                                                                            aaaaTCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGc                     <  1:166307/101‑1 (MQ=255)

                                                                               aTCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTg                  <  1:166293/101‑1 (MQ=255)

                                                                                tCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGc                 >  2:166659/1‑101 (MQ=255)

                                                                                tCGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGc                 <  2:166135/101‑1 (MQ=255)

                                                                                 cGCCCGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTc                         <  1:166111/92‑1 (MQ=255)

                                                                                   cccGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCaa                       <  2:165917/92‑1 (MQ=255)

                                                                                   cccGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTg              <  2:166108/101‑1 (MQ=255)

                                                                                   cccGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTg              >  1:166663/1‑101 (MQ=255)

                                                                                    ccGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGt             <  1:166172/101‑1 (MQ=255)

                                                                                    ccGGCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGt             <  1:166146/101‑1 (MQ=255)

                                                                                      ggCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAAtt                                              <  1:166098/66‑1 (MQ=255)

                                                                                      ggCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTaa           >  2:166670/1‑101 (MQ=255)

                                                                                      ggCGAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTaa           >  1:166669/1‑101 (MQ=255)

                                                                                         gAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTc                         <  1:166341/84‑1 (MQ=255)

                                                                                         gAAGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGc        >  1:166675/1‑101 (MQ=255)

                                                                                          aaGAGACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGcc       >  2:166676/1‑101 (MQ=255)

                                                                                           agagACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCt      <  2:166268/101‑1 (MQ=255)

                                                                                             agaCGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGt    >  2:166683/1‑101 (MQ=255)

                                                                                             agaCGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGt    >  1:166685/1‑101 (MQ=255)

                                                                                             agaCGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGt    <  1:166132/101‑1 (MQ=255)

                                                                                               aCGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTc  >  1:166691/1‑101 (MQ=255)

TATCTTTTTCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTC  >  NC_000913/83289‑83484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`