BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	367,143	A→T	I123N (ATC→AAC)	lacI ←	DNA‑binding transcriptional repressor LacI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	367,143	0	A	T	100.0%	1524.9 / NA	351	I123N (ATC→AAC)	lacI	DNA‑binding transcriptional repressor LacI
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (213/138); total (213/138)

Only 100 of 351 total aligned reads displayed.

TGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACC  >  NC_000913/367045‑367240

 gggTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCa                                                                                                <  2:718339/101‑1 (MQ=255)

 gggTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCa                                                                                                >  2:718792/1‑101 (MQ=255)

   gtgtCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGc                                                                                              >  1:718797/1‑101 (MQ=255)

   gtgtCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGc                                                                                              <  2:718184/101‑1 (MQ=255)

   gtgtCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGc                                                                                              >  2:718796/1‑101 (MQ=255)

       cTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCa                                                                                           <  2:718395/100‑1 (MQ=255)

       cTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCAc                                                                                          >  1:718804/1‑101 (MQ=255)

       cTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCAc                                                                                          >  2:718803/1‑101 (MQ=255)

        tGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACt                                                                                         >  2:718807/1‑101 (MQ=255)

         ggTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTg                                                                                        >  2:718812/1‑101 (MQ=255)

          gTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGa                                                                                       <  2:718327/101‑1 (MQ=255)

           tCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGAc                                                                                      <  2:718126/101‑1 (MQ=255)

           tCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGAc                                                                                      <  2:718386/101‑1 (MQ=255)

            cAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGAcg                                                                                     >  2:718817/1‑101 (MQ=255)

            cAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGAcg                                                                                     >  1:718816/1‑101 (MQ=255)

             agagACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGAcgc                                                                                    >  1:718822/1‑101 (MQ=255)

               agaCATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGt                                                                                  >  2:718825/1‑101 (MQ=255)

                gaCATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGtt                                                                                 <  2:718527/101‑1 (MQ=255)

                gaCATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGtt                                                                                 >  1:718829/1‑101 (MQ=255)

                 aCATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTg                                                                                <  1:718265/101‑1 (MQ=255)

                 aCATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTg                                                                                >  2:718832/1‑101 (MQ=255)

                   aTCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTgcg                                                                              <  2:718165/101‑1 (MQ=255)

                   aTCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTgcg                                                                              >  1:718835/1‑101 (MQ=255)

                     cAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGcgcg                                                                            >  2:718839/1‑101 (MQ=255)

                      aaGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCga                                                                           <  2:718252/101‑1 (MQ=255)

                       aGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCgag                                                                          >  2:718844/1‑101 (MQ=255)

                       aGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCgag                                                                          <  1:718299/101‑1 (MQ=255)

                        gAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCgaga                                                                         >  1:718847/1‑101 (MQ=255)

                         aaaTAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGagaa                                                                        >  2:718849/1‑101 (MQ=255)

                          aaTAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGagaag                                                                       <  1:718364/101‑1 (MQ=255)

                          aaTAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTCACGCGTTGCGCGagaag                                                                       >  2:718853/1‑101 (MQ=255)

                           aTAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGagaaga                                                                      >  1:718856/1‑101 (MQ=255)

                             aaCGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTgcg                                                                              >  2:718866/1‑91 (MQ=255)

                             aaCGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGAtt                                                                    >  2:718860/1‑101 (MQ=255)

                             aaCGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGAtt                                                                    >  1:718863/1‑101 (MQ=255)

                              aCGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATtg                                                                   <  2:718430/101‑1 (MQ=255)

                               cGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATtgt                                                                  <  1:718316/101‑1 (MQ=255)

                               cGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATtgt                                                                  >  1:718870/1‑101 (MQ=255)

                                gCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATtgtg                                                                 >  2:718872/1‑101 (MQ=255)

                                gCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATtgtg                                                                 <  2:718374/101‑1 (MQ=255)

                                 ccGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGc                                                                <  1:718285/101‑1 (MQ=255)

                                   ggAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCAc                                                              >  2:718878/1‑101 (MQ=255)

                                    gAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCAcc                                                             >  1:718880/1‑101 (MQ=255)

                                     aaCATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCAccg                                                            <  2:718613/101‑1 (MQ=255)

                                      aCATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCAccgc                                                           <  2:718547/101‑1 (MQ=255)

                                      aCATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCAccgc                                                           >  2:718884/1‑101 (MQ=255)

                                       cATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCAccgcc                                                          <  2:718508/101‑1 (MQ=255)

                                       cATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCAccgcc                                                          >  1:718886/1‑101 (MQ=255)

                                          tAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCAccg                                                            >  2:718892/1‑96 (MQ=255)

                                           aGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCtt                                                      >  1:718893/1‑101 (MQ=255)

                                           aGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCtt                                                      <  1:718542/101‑1 (MQ=255)

                                             tGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTa                                                    >  2:718898/1‑101 (MQ=255)

                                                  gcagCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGc                                               <  1:718242/101‑1 (MQ=255)

                                                   cagCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGTGCGAGAAGATTGTGCACCGCCGCTTTACAGGCt                                              >  2:718902/1‑101 (MQ=255)

                                                   cagCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCt                                              >  1:718903/1‑101 (MQ=255)

                                                   cagCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCt                                              <  1:718189/101‑1 (MQ=255)

                                                    agCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCtt                                             <  2:718058/101‑1 (MQ=255)

                                                    agCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCtt                                             <  2:718409/101‑1 (MQ=255)

                                                    agCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCtt                                             >  1:718909/1‑101 (MQ=255)

                                                       ttCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGa                                          >  2:718913/1‑101 (MQ=255)

                                                        tCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGAc                                         >  2:718916/1‑101 (MQ=255)

                                                        tCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGAc                                         >  1:718917/1‑101 (MQ=255)

                                                         ccACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGAcg                                        <  2:718585/101‑1 (MQ=255)

                                                          cacaGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGAcgc                                       >  1:718922/1‑101 (MQ=255)

                                                            caGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGAcgccg                                     >  2:718926/1‑101 (MQ=255)

                                                              gCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCTCGAGAAGATTGTGCACCGCCGCttt                                                     <  2:718355/83‑1 (MQ=255)

                                                              gCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCt                                   <  2:718450/101‑1 (MQ=255)

                                                              gCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCt                                   >  1:718928/1‑101 (MQ=255)

                                                                aaTGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTc                                 >  2:718935/1‑101 (MQ=255)

                                                                aaTGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTc                                 <  2:718705/101‑1 (MQ=255)

                                                                aaTGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTc                                 >  1:718934/1‑101 (MQ=255)

                                                                 aTGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCg                                >  1:718938/1‑101 (MQ=255)

                                                                 aTGGCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCg                                <  2:718467/101‑1 (MQ=255)

                                                                    gCATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTc                             >  1:718943/1‑101 (MQ=255)

                                                                     cATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCt                            >  2:718946/1‑101 (MQ=255)

                                                                     cATCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCt                            <  1:718446/101‑1 (MQ=255)

                                                                      aTCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTa                           <  1:718331/101‑1 (MQ=255)

                                                                       tCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTAc                          >  1:718950/1‑101 (MQ=255)

                                                                       tCCTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTAc                          <  1:718077/101‑1 (MQ=255)

                                                                         cTGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCa                        >  2:718955/1‑101 (MQ=255)

                                                                          tGGTCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCAt                       >  1:718956/1‑101 (MQ=255)

                                                                             tCATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGa                    >  1:718961/1‑101 (MQ=255)

                                                                              cATCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGa                                          >  2:718963/1‑78 (MQ=255)

                                                                               aTCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCCCCGCCGCTTTACAGGCt                                              <  1:718399/73‑1 (MQ=255)

                                                                               aTCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGaca                  >  2:718966/1‑101 (MQ=255)

                                                                               aTCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGaca                  <  1:718431/101‑1 (MQ=255)

                                                                                tCCAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGacac                 <  1:718580/101‑1 (MQ=255)

                                                                                 ccAGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGAcacc                <  1:718503/101‑1 (MQ=255)

                                                                                   aGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTc                                            <  2:718282/71‑1 (MQ=255)

                                                                                   aGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGAcaccac              >  1:718974/1‑101 (MQ=255)

                                                                                   aGCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGAcaccac              >  2:718973/1‑101 (MQ=255)

                                                                                    gCGGATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCAccgccg                                                         >  2:718978/1‑57 (MQ=255)

                                                                                      ggATAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGAcaccaccac           >  2:718982/1‑101 (MQ=255)

                                                                                        aTAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGc         >  2:718986/1‑101 (MQ=255)

                                                                                         tAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCt        >  1:718988/1‑101 (MQ=255)

                                                                                         tAGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCt        >  2:718993/1‑101 (MQ=255)

                                                                                          aGTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTg       >  1:718995/1‑101 (MQ=255)

                                                                                           gTTAATGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCg                                           <  2:718240/64‑1 (MQ=255)

                                                                                               aTGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCAcc  >  1:719001/1‑101 (MQ=255)

                                                                                               aTGTTCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCAcc  <  1:718483/101‑1 (MQ=255)

TGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACC  >  NC_000913/367045‑367240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`