Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 99,288 A→G T296A (ACT→GCT)  ftsW → putative lipid II flippase FtsW

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091399,2880AG99.7% 1444.6 / ‑4.7 346T296A (ACT→GCT) ftsWputative lipid II flippase FtsW
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (156/189);  total (156/190)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 355 total aligned reads displayed.

AGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTC  >  NC_000913/99190‑99385
                                                                                                  |                                                                                                 
aGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCt                                                                                                 >  1:198024/1‑101 (MQ=255)
aGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCt                                                                                                 >  2:198023/1‑101 (MQ=255)
aGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCt                                                                                                 <  1:197585/101‑1 (MQ=255)
 gTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTg                                                                                                <  2:197328/101‑1 (MQ=255)
 gTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTg                                                                                                <  1:197382/101‑1 (MQ=255)
  ttAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTg                                                                                                >  2:198029/1‑100 (MQ=255)
  ttAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGa                                                                                               <  1:197262/101‑1 (MQ=255)
   tAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGAc                                                                                              <  2:197553/101‑1 (MQ=255)
    aaCGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACt                                                                                             >  1:198036/1‑101 (MQ=255)
    aaCGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACt                                                                                             <  2:197834/101‑1 (MQ=255)
    aaCGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACt                                                                                             >  2:198033/1‑101 (MQ=255)
     aCGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACtt                                                                                            <  1:197457/101‑1 (MQ=255)
       gCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTa                                                                                          >  2:198039/1‑101 (MQ=255)
       gCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTa                                                                                          <  2:197408/101‑1 (MQ=255)
       gCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTa                                                                                          <  1:197401/101‑1 (MQ=255)
        cAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTAt                                                                                         >  1:198044/1‑101 (MQ=255)
         aaTCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTAtt                                                                                        >  2:198045/1‑101 (MQ=255)
           tCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTAtttt                                                                                      <  1:197547/101‑1 (MQ=255)
           tCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTAtttt                                                                                      >  2:198052/1‑101 (MQ=255)
            cGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTc                                                                                     <  1:197609/101‑1 (MQ=255)
             gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCg                                                                                    >  1:198054/1‑101 (MQ=255)
              cTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGc                                                                                   >  2:198057/1‑101 (MQ=255)
                gATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCa                                                                                 >  1:198061/1‑101 (MQ=255)
                     cGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATc                                                                            <  1:197883/101‑1 (MQ=255)
                      gTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTa                                                                                          <  2:197536/86‑1 (MQ=255)
                      gTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCg                                                                           <  2:197779/101‑1 (MQ=255)
                      gTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCg                                                                           <  1:197621/101‑1 (MQ=255)
                       tttGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCgg                                                                          <  1:197711/101‑1 (MQ=255)
                       tttGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCgg                                                                          >  2:198066/1‑101 (MQ=255)
                          ggTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCga                                                                       >  1:198071/1‑101 (MQ=255)
                            tCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCgaag                                                                     <  2:197277/101‑1 (MQ=255)
                             cgcgGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCgaaga                                                                    <  2:197596/101‑1 (MQ=255)
                             cgcgGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCgaaga                                                                    <  1:197527/101‑1 (MQ=255)
                              gcggcgAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCgaagaa                                                                   >  2:198075/1‑101 (MQ=255)
                                 gcgAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACtt                                                                                            <  2:197221/73‑1 (MQ=255)
                                 gcgAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTg                                                                >  2:198078/1‑101 (MQ=255)
                                  cgAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTgg                                                               <  2:197580/101‑1 (MQ=255)
                                  cgAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTgg                                                               <  2:197383/101‑1 (MQ=255)
                                   gAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCa                                                                                 <  2:197449/82‑1 (MQ=255)
                                   gAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTggg                                                              >  1:198081/1‑101 (MQ=255)
                                    aaCTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTgggg                                                             >  1:198085/1‑101 (MQ=255)
                                      cTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTa                                                           <  2:197516/101‑1 (MQ=255)
                                       tttGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTAt                                                          <  2:197670/101‑1 (MQ=255)
                                       tttGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTAt                                                          <  2:197079/101‑1 (MQ=255)
                                          gggggCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTc                                                       >  2:198087/1‑101 (MQ=255)
                                           ggggGAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCg                                                      <  2:197683/101‑1 (MQ=255)
                                               cAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGgtgt                                                  >  2:198092/1‑101 (MQ=255)
                                                aaGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGtgtg                                                 <  1:197563/101‑1 (MQ=255)
                                                aaGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGtgtg                                                 <  2:197649/101‑1 (MQ=255)
                                                aaGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGtgtg                                                 <  2:197812/101‑1 (MQ=255)
                                                aaGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGtgtg                                                 >  1:198095/1‑101 (MQ=255)
                                                  ggTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTGTGTCGGTgtggt                                               <  2:197349/101‑1 (MQ=255)
                                                  ggTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTgtggt                                               >  2:198098/1‑101 (MQ=255)
                                                   gTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTgtggtg                                              <  2:197762/101‑1 (MQ=255)
                                                     ttAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCt                                            <  1:197836/101‑1 (MQ=255)
                                                      tAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTg                                           >  1:198101/1‑101 (MQ=255)
                                                      tAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTg                                           <  1:197325/101‑1 (MQ=255)
                                                      tAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTg                                           <  2:197737/101‑1 (MQ=255)
                                                        ggTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGc                                         <  2:197634/101‑1 (MQ=255)
                                                        ggTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGc                                         >  2:198105/1‑101 (MQ=255)
                                                          tAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCAc                                       <  2:197897/101‑1 (MQ=255)
                                                          tAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCAc                                       <  1:197663/101‑1 (MQ=255)
                                                           aaCTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACt                                      <  1:197148/101‑1 (MQ=255)
                                                            aCTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACtt                                     <  1:197506/101‑1 (MQ=255)
                                                             cTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACttt                                    <  2:197468/101‑1 (MQ=255)
                                                              tCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACtttt                                   >  2:198113/1‑101 (MQ=255)
                                                              tCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACtttt                                   <  1:197641/101‑1 (MQ=255)
                                                              tCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACtttt                                   >  1:198112/1‑101 (MQ=255)
                                                               cGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTa                                  <  2:197561/101‑1 (MQ=255)
                                                                  tACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACtttt                                   <  1:197595/97‑1 (MQ=255)
                                                                  tACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATg                               >  2:198118/1‑101 (MQ=255)
                                                                   aCAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATgg                              <  1:197495/101‑1 (MQ=255)
                                                                    cAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGt                             <  2:197662/101‑1 (MQ=255)
                                                                    cAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGt                             >  1:198119/1‑101 (MQ=255)
                                                                     aaaaaCTGGAGTATCTGCCGGAAGTGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTa                            <  2:197619/101‑1 (MQ=255)
                                                                     aaaaaCTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTa                            >  2:198125/1‑101 (MQ=255)
                                                                       aaaCTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTAtt                          <  1:197693/101‑1 (MQ=255)
                                                                        aaCTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTAttc                         <  1:197726/101‑1 (MQ=255)
                                                                         aCTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTAttct                        >  1:198130/1‑101 (MQ=255)
                                                                          cTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTAttctt                       <  1:197443/101‑1 (MQ=255)
                                                                          cTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTAttctt                       >  2:198134/1‑101 (MQ=255)
                                                                          cTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTAttctt                       >  1:198133/1‑101 (MQ=255)
                                                                           tGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTAttcttc                      <  2:197492/101‑1 (MQ=255)
                                                                            ggAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTtcg                     >  2:198138/1‑101 (MQ=255)
                                                                               gTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTtcgtcg                  <  1:197739/101‑1 (MQ=255)
                                                                               gTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTtcgtcg                  <  2:197696/101‑1 (MQ=255)
                                                                               gTATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTtcgtcg                  >  2:198144/1‑101 (MQ=255)
                                                                                tATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGc                 >  2:198148/1‑101 (MQ=255)
                                                                                tATCTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGc                 >  1:198147/1‑101 (MQ=255)
                                                                                   cTGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCttt              >  2:198154/1‑101 (MQ=255)
                                                                                    tGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCtttt             >  1:198158/1‑101 (MQ=255)
                                                                                    tGCCGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCtttt             >  2:198159/1‑101 (MQ=255)
                                                                                      ccGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTcg           >  1:198165/1‑101 (MQ=255)
                                                                                       cGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTcgc          <  1:197362/101‑1 (MQ=255)
                                                                                       cGGAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTcgc          >  2:198166/1‑101 (MQ=255)
                                                                                        ggAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTcgcg         >  1:198171/1‑101 (MQ=255)
                                                                                         gAAGCGCACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTcgcgc        >  2:198173/1‑101 (MQ=255)
                                                                                             cgcACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATg    >  1:198179/1‑101 (MQ=255)
                                                                                              gcACGCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGGATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGt   >  2:198181/1‑101 (MQ=255)
                                                                                               cacgCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTc  <  2:197360/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
AGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTC  >  NC_000913/99190‑99385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑