BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,224,612	T→C	E250E (GAA→GAG)	minD ←	Z‑ring positioning protein MinD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,224,612	0	T	C	100.0%	1465.2 / NA	349	E250E (GAA→GAG)	minD	Z‑ring positioning protein MinD
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (190/159); total (190/159)

Only 100 of 370 total aligned reads displayed.

TTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGTTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGTTCACCCTGGTTAGAGGCGCGCA  >  NC_000913/1224514‑1224709

ttcCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGctc                                                                                                 <  2:2363887/101‑1 (MQ=255)

ttcCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGctc                                                                                                 >  2:2364445/1‑101 (MQ=255)

  ccGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGctctt                                                                                               <  2:2363802/101‑1 (MQ=255)

  ccGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGctctt                                                                                               <  2:2363824/101‑1 (MQ=255)

  ccGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGctctt                                                                                               >  2:2364452/1‑101 (MQ=255)

    gcgAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCtcttct                                                                                             >  1:2364455/1‑101 (MQ=255)

        gagaAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCa                                                                                         <  2:2363845/101‑1 (MQ=255)

        gagaAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCa                                                                                         >  1:2364460/1‑101 (MQ=255)

        gagaAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCa                                                                                         <  2:2363981/101‑1 (MQ=255)

          gaaagaaaTCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAAc                                                                                       >  2:2364462/1‑101 (MQ=255)

             agaaaTCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGa                                                                                    <  1:2364099/101‑1 (MQ=255)

              gaaaTCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGAc                                                                                   <  2:2363777/101‑1 (MQ=255)

                 aTCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGtt                                                                                <  1:2363833/101‑1 (MQ=255)

                 aTCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGtt                                                                                >  2:2364468/1‑101 (MQ=255)

                  tCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTc                                                                               >  1:2364471/1‑101 (MQ=255)

                   cGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCt                                                                              <  2:2364044/101‑1 (MQ=255)

                   cGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCt                                                                              >  2:2364474/1‑101 (MQ=255)

                    gAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTa                                                                             <  2:2363939/101‑1 (MQ=255)

                    gAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTa                                                                             <  2:2363588/101‑1 (MQ=255)

                     aGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTAc                                                                            <  2:2363955/101‑1 (MQ=255)

                      gTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACg                                                                           >  1:2364480/1‑101 (MQ=255)

                       tAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGa                                                                                    >  2:2364483/1‑91 (MQ=255)

                        aaTGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGt                                                                         >  1:2364485/1‑101 (MQ=255)

                         aTGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTa                                                                        <  1:2364023/101‑1 (MQ=255)

                          tGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTAt                                                                       <  2:2364087/101‑1 (MQ=255)

                            ccATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCt                                                                     >  1:2364491/1‑101 (MQ=255)

                            ccATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCt                                                                     >  2:2364492/1‑101 (MQ=255)

                              aTAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGc                                                                   >  1:2364497/1‑101 (MQ=255)

                              aTAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGc                                                                   >  2:2364499/1‑101 (MQ=255)

                              aTAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGc                                                                   <  1:2363901/101‑1 (MQ=255)

                                 aCTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTa                                                                >  2:2364505/1‑101 (MQ=255)

                                  cTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAg                                                               <  1:2364318/101‑1 (MQ=255)

                                  cTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAg                                                               >  1:2364507/1‑101 (MQ=255)

                                   ttATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCt                                                                     >  1:2364512/1‑94 (MQ=255)

                                       cctccGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCttt                                                          >  2:2364518/1‑101 (MQ=255)

                                        ctccGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTa                                                         >  1:2364519/1‑101 (MQ=255)

                                        ctccGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCATCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTa                                                         <  2:2363856/101‑1 (MQ=255)

                                          ccGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCttt                                                          >  2:2364526/1‑98 (MQ=255)

                                          ccGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTAcc                                                       >  1:2364525/1‑101 (MQ=255)

                                          ccGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTAcc                                                       <  1:2362936/101‑1 (MQ=255)

                                           cGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTAccc                                                      <  1:2364034/101‑1 (MQ=255)

                                            gAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCg                                                     <  2:2364073/101‑1 (MQ=255)

                                            gAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCg                                                     <  2:2364120/101‑1 (MQ=255)

                                            gAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCg                                                     >  1:2364532/1‑101 (MQ=255)

                                               cAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCAt                                                  <  2:2364068/101‑1 (MQ=255)

                                               cAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCAt                                                  <  1:2364063/101‑1 (MQ=255)

                                                aaGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATc                                                 >  2:2364536/1‑101 (MQ=255)

                                                 aGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCg                                                >  2:2364541/1‑101 (MQ=255)

                                                 aGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCg                                                <  1:2363648/101‑1 (MQ=255)

                                                 aGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCg                                                <  1:2363761/101‑1 (MQ=255)

                                                   cGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGc                                              >  1:2364543/1‑101 (MQ=255)

                                                    gTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCg                                             >  2:2364547/1‑101 (MQ=255)

                                                     tttGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGt                                            <  1:2364192/101‑1 (MQ=255)

                                                     tttGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGt                                            >  1:2364549/1‑101 (MQ=255)

                                                       tGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTa                                          >  2:2364553/1‑101 (MQ=255)

                                                         aGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAAt                                        >  2:2364558/1‑101 (MQ=255)

                                                           gAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGt                                      >  1:2364565/1‑101 (MQ=255)

                                                           gAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGt                                      >  2:2364564/1‑101 (MQ=255)

                                                              gCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCga                                   <  1:2363875/101‑1 (MQ=255)

                                                              gCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCga                                   >  1:2364569/1‑101 (MQ=255)

                                                                cTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCgaga                                 >  2:2364573/1‑101 (MQ=255)

                                                                 tttCTTCTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGaa                                >  2:2364578/1‑101 (MQ=255)

                                                                  ttcttcTCTTCTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAAt                               >  1:2364580/1‑101 (MQ=255)

                                                                   tcttctcttcTTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATg                              <  1:2363792/101‑1 (MQ=255)

                                                                    cttctcttctTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGa                             >  1:2364585/1‑101 (MQ=255)

                                                                     ttctcttctTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGAc                            <  2:2364011/101‑1 (MQ=255)

                                                                       ctcttctTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCg                          >  1:2364590/1‑101 (MQ=255)

                                                                       ctcttctTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCg                          <  1:2364143/101‑1 (MQ=255)

                                                                       ctcttctTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCg                          >  2:2364591/1‑101 (MQ=255)

                                                                       ctcttctTCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCg                          <  2:2363973/101‑1 (MQ=255)

                                                                         cttcttCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGt                        <  2:2363864/101‑1 (MQ=255)

                                                                         cttcttCAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGt                        <  1:2363929/101‑1 (MQ=255)

                                                                          ttcttcAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGtt                       >  1:2364596/1‑101 (MQ=255)

                                                                          ttcttcAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGtt                       <  1:2364049/101‑1 (MQ=255)

                                                                           tcttcAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGTTc                      >  2:2364601/1‑101 (MQ=255)

                                                                            cttcAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGTTCa                     >  2:2364605/1‑101 (MQ=255)

                                                                             ttcAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGTTCAc                    >  1:2364608/1‑101 (MQ=255)

                                                                             ttcAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGTTCAc                    <  2:2364151/101‑1 (MQ=255)

                                                                              tcAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCgg                         <  2:2364019/95‑1 (MQ=255)

                                                                              tcAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGTTCAcc                   >  1:2364612/1‑101 (MQ=255)

                                                                               cAATGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGTTCAccc                  >  2:2364614/1‑101 (MQ=255)

                                                                                aaTGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGTTCACCCt                 <  2:2364313/101‑1 (MQ=255)

                                                                                  tGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGTTCACCCTgg               <  2:2363925/101‑1 (MQ=255)

                                                                                  tGAAGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGTTCACCCTgg               >  2:2364619/1‑101 (MQ=255)

                                                                                   gAAGCGGAAAGGACGCACTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGTTCACCCTGGt              >  1:2364620/1‑101 (MQ=255)

                                                                                    aaGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGtt                       >  2:2364627/1‑91 (MQ=255)

                                                                                    aaGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGTTCACCCTGGtt             <  2:2363713/101‑1 (MQ=255)

                                                                                     aGCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGTTCACCCTGGTTa            >  1:2364629/1‑101 (MQ=255)

                                                                                      gCGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTTGAGAATGACCGGTTCACCCTGGTTag           >  2:2364633/1‑101 (MQ=255)

                                                                                       cGGAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGAcc                           <  1:2364110/84‑1 (MQ=255)

                                                                                        ggAAAGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGTTCACCCTGGTTagag         >  1:2364635/1‑101 (MQ=255)

                                                                                          aaaGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGTTCACCCTGGTTAGAGgc       <  1:2363967/101‑1 (MQ=255)

                                                                                          aaaGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGTTCACCCTGGTTAGAGgc       >  1:2364639/1‑101 (MQ=255)

                                                                                           aaGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGTTCACCCTGGTTAGAGgcg      <  2:2363990/101‑1 (MQ=255)

                                                                                            aGGACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGTTCACCCTGGTTAGAGgcgc     >  1:2364645/1‑101 (MQ=255)

                                                                                             ggACGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGTTCACCCTGGTTAGAGgcgcg    <  2:2364130/101‑1 (MQ=255)

                                                                                               aCGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATg                              <  1:2364041/73‑1 (MQ=255)

                                                                                               aCGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGTTCACCCTGGTTAGAGGCGCGCa  <  1:2364250/101‑1 (MQ=255)

                                                                                               aCGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGTTCACCCTGGTTAGAGGCGCGCa  <  2:2364193/101‑1 (MQ=255)

                                                                                               aCGCTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGTTCACCCTGGTTAGAGGCGCGCa  >  1:2364649/1‑101 (MQ=255)

TTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGTTCTTCTCCCAACAGACGTTCTACGGTATCTGCGTAGGCTTTACCCGCATCGGCGTTAATGTCGAGAATGACCGGTTCACCCTGGTTAGAGGCGCGCA  >  NC_000913/1224514‑1224709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`