BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,335,599	T→C	S10P (TCC→CCC)	ymiC →	protein YmiC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,335,599	0	T	C	98.9%	1382.1 / NA	359	S10P (TCC→CCC)	ymiC	protein YmiC
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (211/144); minor base A (4/0); total (215/144)
Fisher's exact test for biased strand distribution p-value = 1.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 362 total aligned reads displayed.

TTTTAATACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCA  >  NC_000913/1335501‑1335696

ttttAATACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGccc                                                                                                 >  2:2571662/1‑101 (MQ=255)

ttttAATACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGccc                                                                                                 >  1:2571663/1‑101 (MQ=255)

 tttAATACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCt                                                                                                <  2:2571119/101‑1 (MQ=255)

 tttAATACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCt                                                                                                <  1:2571167/101‑1 (MQ=255)

 tttAATACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCt                                                                                                >  1:2571667/1‑101 (MQ=255)

  ttAATACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTg                                                                                               >  1:2571673/1‑101 (MQ=255)

   tAATACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTgg                                                                                              >  1:2571677/1‑101 (MQ=255)

    aaTACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGa                                                                                             >  2:2571679/1‑101 (MQ=255)

     aTACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGAt                                                                                            <  2:2571071/101‑1 (MQ=255)

     aTACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGAt                                                                                            <  2:2571260/101‑1 (MQ=255)

     aTACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGAt                                                                                            <  1:2571157/101‑1 (MQ=255)

      tACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATg                                                                                           >  1:2571684/1‑101 (MQ=255)

        cacCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATggg                                                                                         >  1:2571689/1‑101 (MQ=255)

        cacCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATggg                                                                                         >  2:2571690/1‑101 (MQ=255)

         acCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGgc                                                                                        >  1:2571694/1‑101 (MQ=255)

           cGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGgcgc                                                                                      <  1:2570841/101‑1 (MQ=255)

           cGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGgcgc                                                                                      >  2:2571697/1‑101 (MQ=255)

           cGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGgcgc                                                                                      <  1:2571233/101‑1 (MQ=255)

           cGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGgcgc                                                                                      <  2:2571273/101‑1 (MQ=255)

            gcagcaATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGcgcg                                                                                     <  1:2571135/101‑1 (MQ=255)

            gcagcaATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGcgcg                                                                                     <  2:2571211/101‑1 (MQ=255)

            gcagcaATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGcgcg                                                                                     >  1:2571700/1‑101 (MQ=255)

             cagcaATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGt                                                                                    <  1:2571245/101‑1 (MQ=255)

               gcaATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGttt                                                                                  <  1:2570642/101‑1 (MQ=255)

                caATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGtttt                                                                                 >  2:2571707/1‑101 (MQ=255)

                caATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGtttt                                                                                 <  2:2570696/101‑1 (MQ=255)

                caATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGtttt                                                                                 >  1:2571708/1‑101 (MQ=255)

                  aTAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTtct                                                                               <  2:2571152/101‑1 (MQ=255)

                   tAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTtctc                                                                              >  1:2571713/1‑101 (MQ=255)

                     aCAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTg                                                                            >  1:2571719/1‑101 (MQ=255)

                     aCAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTg                                                                            <  2:2570989/101‑1 (MQ=255)

                     aCAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTg                                                                            >  2:2571718/1‑101 (MQ=255)

                      cAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGt                                                                           <  1:2571144/101‑1 (MQ=255)

                      cAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGt                                                                           >  2:2571723/1‑101 (MQ=255)

                       aGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTc                                                                          >  1:2571725/1‑101 (MQ=255)

                       aGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTc                                                                          >  1:2571729/1‑101 (MQ=255)

                        gCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCg                                                                         <  2:2570966/101‑1 (MQ=255)

                        gCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCg                                                                         <  1:2571010/101‑1 (MQ=255)

                          ttGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGAt                                                                       <  1:2571222/101‑1 (MQ=255)

                           tGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATg                                                                      >  2:2571735/1‑101 (MQ=255)

                             aGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGct                                                                    >  2:2571741/1‑101 (MQ=255)

                             aGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGct                                                                    <  1:2570992/101‑1 (MQ=255)

                             aGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGct                                                                    >  1:2571740/1‑101 (MQ=255)

                               ttATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGctct                                                                  >  1:2571744/1‑101 (MQ=255)

                                tATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCtctt                                                                 >  2:2571750/1‑101 (MQ=255)

                                 aTCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCtcttc                                                                >  1:2571753/1‑101 (MQ=255)

                                    tcAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTgg                                                             >  2:2571757/1‑101 (MQ=255)

                                    tcAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTgg                                                             <  2:2571029/101‑1 (MQ=255)

                                     cAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTggg                                                            >  2:2571761/1‑101 (MQ=255)

                                      aaCACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGc                                                           >  1:2571762/1‑101 (MQ=255)

                                         acaAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGa                                                        >  1:2571766/1‑101 (MQ=255)

                                           aaaaTAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAAc                                                      >  1:2571776/1‑101 (MQ=255)

                                           aaaaTAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAAc                                                      >  1:2571773/1‑101 (MQ=255)

                                             aataatAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAActc                                                    >  2:2571780/1‑101 (MQ=255)

                                              ataataACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAActcc                                                   >  1:2571782/1‑101 (MQ=255)

                                               taataaCCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAActcct                                                  >  1:2571786/1‑101 (MQ=255)

                                                 ataaCCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCctct                                                >  1:2571790/1‑101 (MQ=255)

                                                    aCCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGa                                             >  2:2571796/1‑101 (MQ=255)

                                                     ccGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGAt                                            >  1:2571797/1‑101 (MQ=255)

                                                         tAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCAtt                                        >  1:2571803/1‑101 (MQ=255)

                                                          aaGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTa                                       >  1:2571806/1‑101 (MQ=255)

                                                           aGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTAc                                      >  2:2571809/1‑101 (MQ=255)

                                                            gggTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACt                                     <  2:2571282/101‑1 (MQ=255)

                                                             ggTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTc                                    <  2:2571199/101‑1 (MQ=255)

                                                             ggTGTAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTc                                    >  1:2571811/1‑101 (MQ=255)

                                                               tgtAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCAc                                  <  1:2571063/101‑1 (MQ=255)

                                                                 tAGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTg                                >  2:2571815/1‑101 (MQ=255)

                                                                  aGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGa                               <  1:2571286/101‑1 (MQ=255)

                                                                  aGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGa                               <  1:2571340/101‑1 (MQ=255)

                                                                  aGCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGa                               <  2:2571327/101‑1 (MQ=255)

                                                                   gCCTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGAt                              >  1:2571821/1‑101 (MQ=255)

                                                                     cTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATcc                            >  2:2571824/1‑101 (MQ=255)

                                                                     cTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATcc                            >  1:2571826/1‑101 (MQ=255)

                                                                     cTATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATcc                            <  1:2571201/101‑1 (MQ=255)

                                                                      tATGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCt                           <  2:2571143/101‑1 (MQ=255)

                                                                       aTGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCtt                          <  2:2571502/101‑1 (MQ=255)

                                                                       aTGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCtt                          <  1:2571027/101‑1 (MQ=255)

                                                                       aTGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCtt                          <  1:2571479/101‑1 (MQ=255)

                                                                        tGATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCAATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTg                         >  1:2571832/1‑101 (MQ=255)

                                                                         gATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGa                        <  2:2571407/101‑1 (MQ=255)

                                                                         gATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGa                        >  1:2571835/1‑101 (MQ=255)

                                                                         gATCAACACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGa                        >  2:2571836/1‑101 (MQ=255)

                                                                             aaCACAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGAcccc                    >  1:2571842/1‑101 (MQ=255)

                                                                              acacAAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCg                   >  2:2571845/1‑101 (MQ=255)

                                                                                acaAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCt                 <  2:2571370/101‑1 (MQ=255)

                                                                                acaAATATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCt                 <  2:2571346/101‑1 (MQ=255)

                                                                                  aaaTATGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTgc               >  2:2571849/1‑101 (MQ=255)

                                                                                    atatGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTgcgg             >  2:2571855/1‑101 (MQ=255)

                                                                                    atatGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTgcgg             >  1:2571854/1‑101 (MQ=255)

                                                                                     tatGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTgcggc            <  2:2571248/101‑1 (MQ=255)

                                                                                     tatGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTgcggc            <  2:2571225/101‑1 (MQ=255)

                                                                                     tatGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTgcggc            >  2:2571860/1‑101 (MQ=255)

                                                                                      atGAAATATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTgcggcg           >  1:2571863/1‑101 (MQ=255)

                                                                                          aaTATTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCggggg       >  2:2571867/1‑100 (MQ=255)

                                                                                            tatTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCgggg        >  1:2571874/1‑98 (MQ=255)

                                                                                            tatTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTg     >  1:2571871/1‑101 (MQ=255)

                                                                                             atTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTgcgg             >  2:2571878/1‑92 (MQ=255)

                                                                                             atTGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGt    <  2:2571305/101‑1 (MQ=255)

                                                                                              ttGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTc   <  2:2571185/101‑1 (MQ=255)

                                                                                               tGGCCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCa  <  1:2571092/101‑1 (MQ=255)

TTTTAATACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCA  >  NC_000913/1335501‑1335696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`