BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	127,779	A→G	intergenic (+192/‑133)	aceF → / → lpd	pyruvate dehydrogenase, E2 subunit/lipoamide dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	127,779	0	A	G	100.0%	1656.1 / NA	393	intergenic (+192/‑133)	aceF/lpd	pyruvate dehydrogenase, E2 subunit/lipoamide dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (250/143); total (250/143)

Only 100 of 401 total aligned reads displayed.

GTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCAGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAACGCCGCTGCGGCCTGAAAGACGACGG  >  NC_000913/127681‑127876

gttTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTgcggc                                                                                                 <  1:252616/101‑1 (MQ=255)

gttTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTgcggc                                                                                                 >  2:253557/1‑101 (MQ=255)

   tAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAg                                                                                              >  1:253566/1‑101 (MQ=255)

   tAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAg                                                                                              >  2:253565/1‑101 (MQ=255)

    aaaaaTTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTTACATACCCTAT‑GATTTCTGGGTGCGGCAAgg                                                                                             >  1:253572/1‑100 (MQ=255)

    aaaaaTTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAgg                                                                                             <  2:252640/101‑1 (MQ=255)

     aaaaTTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGt                                                                                            >  2:253577/1‑101 (MQ=255)

     aaaaTTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGt                                                                                            <  1:253041/101‑1 (MQ=255)

      aaaTTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTa                                                                                           >  1:253579/1‑101 (MQ=255)

        aTTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGc                                                                                         <  1:253222/101‑1 (MQ=255)

         ttGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCa                                                                                        >  2:253586/1‑101 (MQ=255)

          tGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCaa                                                                                       >  2:253590/1‑101 (MQ=255)

          tGTTAACAA‑TTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAg                                                                                      <  2:253120/101‑1 (MQ=255)

           gTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAg                                                                                      >  1:253595/1‑101 (MQ=255)

            ttAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAgc                                                                                     >  2:253597/1‑101 (MQ=255)

            ttAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAgc                                                                                     <  2:252763/101‑1 (MQ=255)

              aaCAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAgcgc                                                                                   >  2:253602/1‑101 (MQ=255)

                cAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCa                                                                                 >  2:253607/1‑101 (MQ=255)

                cAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCa                                                                                 >  1:253611/1‑101 (MQ=255)

                 aaTTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAg                                                                                >  1:253616/1‑101 (MQ=255)

                  aTTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGa                                                                               >  2:253618/1‑101 (MQ=255)

                   ttttGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGaa                                                                              >  1:253623/1‑101 (MQ=255)

                   ttttGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGaa                                                                              <  2:253075/101‑1 (MQ=255)

                     ttGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATc                                                                            >  1:253629/1‑101 (MQ=255)

                     ttGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATc                                                                            <  1:253012/101‑1 (MQ=255)

                      tGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATcc                                                                           >  2:253630/1‑101 (MQ=255)

                       gTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATccc                                                                          >  2:253635/1‑101 (MQ=255)

                        tAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATcccc                                                                         >  1:253640/1‑101 (MQ=255)

                         aaaaTACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCa                                                                        >  1:253647/1‑101 (MQ=255)

                         aaaaTACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCa                                                                        >  2:253645/1‑101 (MQ=255)

                           aaTACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAgg                                                                      <  1:252844/101‑1 (MQ=255)

                           aaTACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAgg                                                                      >  2:253651/1‑101 (MQ=255)

                             tACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAg                                                                    >  2:253658/1‑101 (MQ=255)

                              aCCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGc                                                                   <  1:253107/101‑1 (MQ=255)

                              aCCGACGGATAGAACGACCCGGGGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGc                                                                   >  1:253660/1‑101 (MQ=255)

                               ccGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCt                                                                  >  1:253664/1‑101 (MQ=255)

                                cGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCtt                                                                 >  2:253668/1‑101 (MQ=255)

                                 gACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTa                                                                <  2:252965/101‑1 (MQ=255)

                                    ggATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACAt                                                             >  2:253674/1‑101 (MQ=255)

                                      aTAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACAtaa                                                           >  1:253677/1‑101 (MQ=255)

                                      aTAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACAtaa                                                           >  2:253680/1‑101 (MQ=255)

                                        aGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACAtaagt                                                         <  2:252848/101‑1 (MQ=255)

                                        aGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACAtaagt                                                         >  1:253684/1‑101 (MQ=255)

                                         gAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACAtaagta                                                        <  2:253087/101‑1 (MQ=255)

                                           aCGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACAtaagtaag                                                      >  1:253690/1‑101 (MQ=255)

                                            cGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATaagtaagt                                                     >  2:253691/1‑101 (MQ=255)

                                             gACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTg                                                    <  2:253349/101‑1 (MQ=255)

                                              aCCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGa                                                   <  1:252769/101‑1 (MQ=255)

                                              aCCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGa                                                   >  1:253697/1‑101 (MQ=255)

                                                ccGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACt                                                 >  2:253702/1‑101 (MQ=255)

                                                 cGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTg                                                <  1:253028/101‑1 (MQ=255)

                                                 cGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTg                                                <  1:252911/101‑1 (MQ=255)

                                                  ggtggtggtTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTgg                                               >  1:253706/1‑101 (MQ=255)

                                                   gtggtggtTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTggg                                              >  2:253710/1‑101 (MQ=255)

                                                   gtggtggtTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTggg                                              <  2:253005/101‑1 (MQ=255)

                                                   gtggtggtTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTggg                                              >  1:253711/1‑101 (MQ=255)

                                                    tggtggtTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTgggg                                             >  1:253716/1‑101 (MQ=255)

                                                     ggtggtTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGt                                            >  2:253719/1‑101 (MQ=255)

                                                      gtggtTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTg                                           >  2:253723/1‑101 (MQ=255)

                                                        ggtTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAg                                         <  1:253184/101‑1 (MQ=255)

                                                          ttAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAggg                                       >  2:253730/1‑101 (MQ=255)

                                                          ttAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAggg                                       >  1:253733/1‑101 (MQ=255)

                                                          ttAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAggg                                       <  1:253158/101‑1 (MQ=255)

                                                           tAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGc                                      >  2:253735/1‑101 (MQ=255)

                                                           tAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGc                                      <  2:253154/101‑1 (MQ=255)

                                                             gggTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGt                                    >  1:253742/1‑101 (MQ=255)

                                                               gTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGa                                  >  2:253746/1‑101 (MQ=255)

                                                               gTATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGa                                  >  1:253748/1‑101 (MQ=255)

                                                                tATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGaa                                 <  2:253227/101‑1 (MQ=255)

                                                                tATTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGaa                                 <  2:253181/101‑1 (MQ=255)

                                                                 aTTACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAg                                >  2:253754/1‑101 (MQ=255)

                                                                  ttACTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGc                               >  1:253755/1‑101 (MQ=255)

                                                                     cTTCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTaa                            >  2:253761/1‑101 (MQ=255)

                                                                      ttCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAAc                           <  2:252979/101‑1 (MQ=255)

                                                                       tCACATACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAAcg                          <  2:252934/101‑1 (MQ=255)

                                                                        cacaTACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAAcgc                         <  1:253067/101‑1 (MQ=255)

                                                                        cacaTACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAAcgc                         >  2:253766/1‑101 (MQ=255)

                                                                         acaTACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAAcgcc                        >  1:253767/1‑101 (MQ=255)

                                                                         acaTACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAAcgcc                        <  2:253245/101‑1 (MQ=255)

                                                                          caTACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAAcgccg                       >  1:253772/1‑101 (MQ=255)

                                                                           aTACCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAAcgccgc                      >  1:253776/1‑101 (MQ=255)

                                                                             aCCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAACGCCGCTg                    >  2:253783/1‑101 (MQ=255)

                                                                             aCCCTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAACGCCGCTg                    >  1:253782/1‑101 (MQ=255)

                                                                               ccTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAACGCCGCTGCg                  <  1:253460/101‑1 (MQ=255)

                                                                               ccTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAACGCCGCTGCg                  >  2:253788/1‑101 (MQ=255)

                                                                                cTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAACGCCGCTGCgg                 >  2:253794/1‑101 (MQ=255)

                                                                                cTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAACGCCGCTGCgg                 <  1:253132/101‑1 (MQ=255)

                                                                                cTATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAAGCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAACGCCGCTg                    <  2:253057/98‑1 (MQ=255)

                                                                                 tATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAACGCCGCTGCGGc                <  1:253299/101‑1 (MQ=255)

                                                                                 tATGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAACGCCGCTGCGGc                >  1:253796/1‑101 (MQ=255)

                                                                                   tGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAACGCCGCTGCGGCCt              <  2:252903/101‑1 (MQ=255)

                                                                                   tGGATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAACGCCGCTGCGGCCt              <  2:253102/101‑1 (MQ=255)

                                                                                    ggATTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAACGCCGCTGCGGCCTg             <  2:253476/101‑1 (MQ=255)

                                                                                      aTTTCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAACGCCGCTGCGGCCTGaa           >  2:253800/1‑101 (MQ=255)

                                                                                       tttCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAACGCCGCTGCGGCCTGaaa          <  2:253025/101‑1 (MQ=255)

                                                                                       tttCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAACGCCGCTGCGGCCTGaaa          >  2:253806/1‑101 (MQ=255)

                                                                                        ttCTGGGTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAACGCCGCTGCGGCCTGAAAg         >  1:253809/1‑101 (MQ=255)

                                                                                            gggTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAACGCCGCTGCGGCCTGAAAgacga     <  1:252941/101‑1 (MQ=255)

                                                                                            gggTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAACGCCGCTGCGGCCTGAAAgacga     >  2:253812/1‑101 (MQ=255)

                                                                                             ggTGCGGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAACGCCGCTGCGGCCTGAAAgacgac    <  2:253259/101‑1 (MQ=255)

GTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCACATACCCTATGGATTTCTGGGTGCAGCAAGGTAGCAAGCGCCAGAATCCCCAGGAGCTTACATAAGTAAGTGACTGGGGTGAGGGCGTGAAGCTAACGCCGCTGCGGCCTGAAAGACGACGG  >  NC_000913/127681‑127876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`