Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,569,394 A→G S366P (TCC→CCC)  gadC ← L‑glutamate:4‑aminobutyrate antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,569,3940AG99.7% 1176.9 / ‑5.4 285S366P (TCC→CCC) gadCL‑glutamate:4‑aminobutyrate antiporter
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (143/141);  total (144/141)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 307 total aligned reads displayed.

GTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTG  >  NC_000913/1569296‑1569491
                                                                                                  |                                                                                                 
  cAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCAtg                                                                                               >  2:3016192/1‑101 (MQ=255)
   aGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCAtgt                                                                                              >  2:3016200/1‑101 (MQ=255)
   aGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCAtgt                                                                                              >  1:3016195/1‑101 (MQ=255)
    ggATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCAtgtt                                                                                             <  1:3015686/101‑1 (MQ=255)
    ggATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCAtgtt                                                                                             <  2:3015355/101‑1 (MQ=255)
    ggATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCAtgtt                                                                                             >  1:3016201/1‑101 (MQ=255)
     gATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCAtgttg                                                                                            >  2:3016205/1‑101 (MQ=255)
     gATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCAtgttg                                                                                            <  2:3015861/101‑1 (MQ=255)
        gTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTa                                                                                         >  1:3016208/1‑101 (MQ=255)
         tttAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTAc                                                                                        <  1:3015883/101‑1 (MQ=255)
          ttAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTAcc                                                                                       <  2:3015785/101‑1 (MQ=255)
           tAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCg                                                                                      >  2:3016211/1‑101 (MQ=255)
            aaGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTa                                                                                         >  1:3016214/1‑97 (MQ=255)
               aaCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCAc                                                                                  <  1:3015333/101‑1 (MQ=255)
                aCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCAcc                                                                                 <  1:3015459/101‑1 (MQ=255)
                aCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCAcc                                                                                 >  2:3016219/1‑101 (MQ=255)
                 ccAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCg                                                                                <  1:3015625/101‑1 (MQ=255)
                 ccAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCg                                                                                >  1:3016220/1‑101 (MQ=255)
                  cAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCgg                                                                               >  1:3016223/1‑101 (MQ=255)
                   aaCACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGt                                                                              <  2:3015663/101‑1 (MQ=255)
                    acacAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTa                                                                             <  1:3015829/101‑1 (MQ=255)
                    acacAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTa                                                                             <  1:3015511/101‑1 (MQ=255)
                      acaATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTAtt                                                                           >  1:3016229/1‑101 (MQ=255)
                      acaATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTAtt                                                                           >  2:3016226/1‑101 (MQ=255)
                        aaTGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTgg                                                                         <  2:3015827/101‑1 (MQ=255)
                         aTGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGt                                                                        >  2:3016235/1‑101 (MQ=255)
                         aTGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGt                                                                        >  1:3016234/1‑101 (MQ=255)
                           gTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGa                                                                      <  1:3015643/101‑1 (MQ=255)
                            tAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAg                                                                     <  1:3015759/101‑1 (MQ=255)
                            tAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAg                                                                     >  1:3016239/1‑101 (MQ=255)
                             aGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAgg                                                                    >  1:3016242/1‑101 (MQ=255)
                               ccAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGgat                                                                  >  1:3016246/1‑101 (MQ=255)
                                cAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGgatg                                                                 >  2:3016250/1‑101 (MQ=255)
                                 aaTAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGgatga                                                                <  2:3015237/101‑1 (MQ=255)
                                 aaTAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGgatga                                                                >  1:3016251/1‑101 (MQ=255)
                                 aaTAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGgatga                                                                <  2:3015698/101‑1 (MQ=255)
                                   tAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATc                                                              >  2:3016256/1‑101 (MQ=255)
                                    aaaCAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCa                                                             >  1:3016258/1‑101 (MQ=255)
                                     aaCAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCaa                                                            <  2:3015455/101‑1 (MQ=255)
                                       cAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAAcg                                                          <  2:3015566/101‑1 (MQ=255)
                                        aGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAAcgc                                                         >  1:3016262/1‑101 (MQ=255)
                                        aGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAAcgc                                                         >  2:3016263/1‑101 (MQ=255)
                                        aGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAAcgc                                                         <  2:3015612/101‑1 (MQ=255)
                                          cATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGa                                                       >  1:3016266/1‑101 (MQ=255)
                                            tGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATa                                                     <  2:3016176/101‑1 (MQ=255)
                                            tGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATa                                                     <  1:3015591/101‑1 (MQ=255)
                                            tGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATa                                                     <  2:3015674/101‑1 (MQ=255)
                                            tGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATa                                                     <  2:3015897/101‑1 (MQ=255)
                                            tGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATa                                                     >  1:3016269/1‑101 (MQ=255)
                                              aaaTAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGa                                                   >  1:3016272/1‑101 (MQ=255)
                                               aaTAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGAc                                                  >  1:3016276/1‑101 (MQ=255)
                                                 tAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGt                                                <  1:3015848/101‑1 (MQ=255)
                                                 tAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGt                                                >  2:3016280/1‑101 (MQ=255)
                                                  aaGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTa                                               >  1:3016283/1‑101 (MQ=255)
                                                   aGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTaa                                              <  1:3015739/101‑1 (MQ=255)
                                                   aGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTaa                                              >  1:3016287/1‑101 (MQ=255)
                                                     cgcacaGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATc                                            <  2:3015817/99‑1 (MQ=255)
                                                      acacaGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAgg                                                                    <  1:3015551/76‑1 (MQ=255)
                                                      acacaGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCa                                           <  2:3015807/101‑1 (MQ=255)
                                                          aGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAg                                       >  2:3016290/1‑101 (MQ=255)
                                                          aGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAg                                       >  1:3016291/1‑101 (MQ=255)
                                                           gATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGc                                      <  1:3015818/101‑1 (MQ=255)
                                                           gATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGc                                      <  1:3015787/101‑1 (MQ=255)
                                                             tAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTg                                    <  2:3015846/101‑1 (MQ=255)
                                                             tAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTg                                    <  1:3015778/101‑1 (MQ=255)
                                                             tAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTg                                    >  1:3016294/1‑101 (MQ=255)
                                                               aaTCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCg                                  <  2:3015990/101‑1 (MQ=255)
                                                                 tCACCACCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGaa                                >  2:3016299/1‑101 (MQ=255)
                                                                  caccacCGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGaaa                               <  1:3015649/101‑1 (MQ=255)
                                                                   accaccGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAg                                       >  1:3016303/1‑92 (MQ=255)
                                                                    ccaccGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAAcgc                                                         <  2:3015917/73‑1 (MQ=255)
                                                                    ccaccGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATg                             >  2:3016304/1‑101 (MQ=255)
                                                                     caccGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGa                            >  1:3016310/1‑101 (MQ=255)
                                                                     caccGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGa                            <  2:3015633/101‑1 (MQ=255)
                                                                     caccGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGa                            >  2:3016308/1‑101 (MQ=255)
                                                                     caccGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGa                            <  1:3016097/101‑1 (MQ=255)
                                                                      accGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGAc                           <  1:3016024/101‑1 (MQ=255)
                                                                       ccGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGAcc                          <  2:3015923/101‑1 (MQ=255)
                                                                        cGTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCa                         >  1:3016314/1‑101 (MQ=255)
                                                                         gTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAAt                              <  2:3015479/95‑1 (MQ=255)
                                                                         gTCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAg                        >  2:3016316/1‑101 (MQ=255)
                                                                          tCAGCGCCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGc                       <  2:3016017/101‑1 (MQ=255)
                                                                             gcgcCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGtt                    <  1:3015903/101‑1 (MQ=255)
                                                                              cgcCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTa                   >  2:3016322/1‑101 (MQ=255)
                                                                              cgcCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTa                   >  1:3016321/1‑101 (MQ=255)
                                                                              cgcCAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTa                   <  1:3015944/101‑1 (MQ=255)
                                                                                ccAGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTAcc                 <  2:3015763/101‑1 (MQ=255)
                                                                                  aGTGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCgg               <  1:3015869/101‑1 (MQ=255)
                                                                                    tGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTa             >  1:3016328/1‑101 (MQ=255)
                                                                                    tGCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTa             >  2:3016327/1‑101 (MQ=255)
                                                                                     gCGATCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTAc            <  1:3015711/101‑1 (MQ=255)
                                                                                        aTCAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGcc         >  2:3016332/1‑101 (MQ=255)
                                                                                          cAGGAAGGGCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCAt       >  1:3016335/1‑101 (MQ=255)
                                                                                           aggaagggCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCAtt      <  1:3015991/101‑1 (MQ=255)
                                                                                           aggaagggCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCAtt      >  2:3016339/1‑101 (MQ=255)
                                                                                            ggaagggCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCAttt     >  1:3016341/1‑101 (MQ=255)
                                                                                             gaagggCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCAtttt    <  2:3015745/101‑1 (MQ=255)
                                                                                             gaagggCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCAtttt    <  2:3015704/101‑1 (MQ=255)
                                                                                              aaggcCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCAttttt   <  1:3015801/101‑1 (MQ=255)
                                                                                               agggCATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTg  <  2:3015871/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
GTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTG  >  NC_000913/1569296‑1569491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑