BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,250,434	T→C	F240L (TTC→CTC)	yeiH →	conserved inner membrane protein YeiH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,250,434	0	T	C	100.0%	1406.8 / NA	333	F240L (TTC→CTC)	yeiH	conserved inner membrane protein YeiH
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (172/161); total (172/161)

Only 100 of 338 total aligned reads displayed.

AGTGGCGCAGGTGGTGGCGGCAGGTCATGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGCCATCTTGTTCAT  >  NC_000913/2250337‑2250531

   ggCGCAGGTGGTGGCGGCAGGTCATGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCCCa                                                                                             <  1:4311540/101‑1 (MQ=255)

     cgcAGGTGGTGGCGGCAGGTCATGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATc                                                                                           >  1:4311980/1‑101 (MQ=255)

       cAGGTGGTGGCGGCAGGTCATGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCct                                                                                         <  2:4311495/101‑1 (MQ=255)

       cAGGTGGTGGCGGCAGGTCATGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCct                                                                                         <  1:4311569/101‑1 (MQ=255)

        aGGTGGTGGCGGCAGGCCATGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCctg                                                                                        >  2:4311984/1‑101 (MQ=255)

          gtggtgGCGGCAGGTCATGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCctgct                                                                                      <  2:4311388/101‑1 (MQ=255)

          gtggtgGCGGCAGGTCATGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCctgct                                                                                      >  1:4311985/1‑101 (MQ=255)

            ggtggCGGCAGGTCATGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTgg                                                                                    <  1:4311759/101‑1 (MQ=255)

             gtggCGGCAGGTCATGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTggc                                                                                   <  2:4311232/101‑1 (MQ=255)

             gtggCGGCAGGTCATGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTggc                                                                                   <  2:4311477/101‑1 (MQ=255)

             gtggCGGCAGGTCATGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTggc                                                                                   <  2:4311631/101‑1 (MQ=255)

             gtggCGGCAGGTCATGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTggc                                                                                   <  1:4311617/101‑1 (MQ=255)

               ggcggcAGGTCATGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTggcgg                                                                                 <  1:4311473/101‑1 (MQ=255)

                gcggcAGGTCATGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTggcggc                                                                                >  2:4311991/1‑101 (MQ=255)

                 cggcAGGTCATGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGgcggcg                                                                               >  2:4311995/1‑101 (MQ=255)

                  ggcAGGTCATGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGgcgc                                                                              >  1:4311997/1‑101 (MQ=255)

                       gTCATGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGtt                                                                         >  1:4312002/1‑101 (MQ=255)

                        tCATGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTa                                                                        >  2:4312003/1‑101 (MQ=255)

                        tCATGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTa                                                                        <  1:4311605/101‑1 (MQ=255)

                          aTGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTaaa                                                                      <  2:4311638/101‑1 (MQ=255)

                          aTGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTaaa                                                                      >  1:4312006/1‑101 (MQ=255)

                            gCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACa                                                                    >  2:4312009/1‑101 (MQ=255)

                             ccATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAg                                                                   <  1:4311108/101‑1 (MQ=255)

                                  aGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTc                                                              >  1:4312015/1‑101 (MQ=255)

                                    cccGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTg                                                            >  2:4312019/1‑101 (MQ=255)

                                    cccGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTg                                                            <  1:4311277/101‑1 (MQ=255)

                                    cccGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTg                                                            <  2:4311683/101‑1 (MQ=255)

                                     ccGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTgg                                                           >  1:4312020/1‑101 (MQ=255)

                                     ccGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTg                                                            <  1:4311522/100‑1 (MQ=255)

                                      cGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCt                                                             >  2:4312026/1‑98 (MQ=255)

                                       ggATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTgggg                                                         <  1:4311641/101‑1 (MQ=255)

                                       ggATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTgggg                                                         <  1:4311505/101‑1 (MQ=255)

                                        gATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGc                                                        >  1:4312028/1‑101 (MQ=255)

                                        gATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGc                                                        <  2:4311642/101‑1 (MQ=255)

                                        gATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGc                                                        <  1:4311386/101‑1 (MQ=255)

                                         aTGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCg                                                       >  1:4312033/1‑101 (MQ=255)

                                         aTGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCg                                                       <  2:4311695/101‑1 (MQ=255)

                                         aTGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCg                                                       >  2:4312030/1‑101 (MQ=255)

                                          tGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGa                                                      <  2:4311443/101‑1 (MQ=255)

                                             ggAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACa                                                   >  1:4312041/1‑101 (MQ=255)

                                             ggAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACa                                                   >  2:4312040/1‑101 (MQ=255)

                                              gAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAg                                                  <  2:4311425/101‑1 (MQ=255)

                                               aaaaCGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAgc                                                 <  1:4311217/101‑1 (MQ=255)

                                               aaaaCGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAgc                                                 <  1:4311421/101‑1 (MQ=255)

                                               aaaaCGCAGCAGTTATTTACAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAgc                                                 <  2:4311592/101‑1 (MQ=255)

                                                aaaCGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCt                                                                 <  1:4311446/84‑1 (MQ=255)

                                                 aaCGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAgcgg                                               >  1:4312048/1‑101 (MQ=255)

                                                 aaCGCAGCAGTTATTTACAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAgcgg                                               >  2:4312047/1‑101 (MQ=255)

                                                  aCGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAgcggc                                              <  2:4311930/101‑1 (MQ=255)

                                                   cGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGc                                                        <  2:4311434/90‑1 (MQ=255)

                                                   cGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAgcggcg                                             >  2:4312052/1‑101 (MQ=255)

                                                    gcagcaGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCga                                            <  2:4311290/101‑1 (MQ=255)

                                                     cagcagTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCgag                                           <  2:4311581/101‑1 (MQ=255)

                                                     cagcagTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCgag                                           <  2:4311554/101‑1 (MQ=255)

                                                      agcagTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCgaga                                          >  2:4312057/1‑101 (MQ=255)

                                                      agcagTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCgaga                                          >  1:4312059/1‑101 (MQ=255)

                                                       gcagTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCgtg                                                                           >  2:4312062/1‑67 (MQ=255)

                                                       gcagTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGaa                                         <  2:4311533/101‑1 (MQ=255)

                                                        cagTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGaaa                                        >  2:4312068/1‑101 (MQ=255)

                                                        cagTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGaaa                                        >  1:4312066/1‑101 (MQ=255)

                                                         agTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGaaaa                                       <  2:4311405/101‑1 (MQ=255)

                                                          gTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAg                                      <  1:4311513/101‑1 (MQ=255)

                                                            tATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCa                                    >  1:4312074/1‑101 (MQ=255)

                                                             aTTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCaa                                   >  2:4312075/1‑101 (MQ=255)

                                                              tttCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCaaa                                  >  2:4312080/1‑101 (MQ=255)

                                                                tCCAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAAt                                >  1:4312083/1‑101 (MQ=255)

                                                                 ccAAAATGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATc                               <  2:4311489/101‑1 (MQ=255)

                                                                   aaaaTGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCAc                             >  2:4312088/1‑101 (MQ=255)

                                                                   aaaaTGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCAc                             >  1:4312090/1‑101 (MQ=255)

                                                                    aaaTGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCaaaa                                 >  2:4312093/1‑96 (MQ=255)

                                                                     aaTGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTa                           >  1:4312097/1‑101 (MQ=255)

                                                                     aaTGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTa                           <  1:4311622/101‑1 (MQ=255)

                                                                      aTGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTAt                          >  2:4312101/1‑101 (MQ=255)

                                                                      aTGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTAt                          >  1:4312100/1‑101 (MQ=255)

                                                                      aTGCTGCGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTAt                          <  2:4311463/101‑1 (MQ=255)

                                                                         ctgcGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTcc                       >  2:4312106/1‑101 (MQ=255)

                                                                          tgcGCGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCg                      >  1:4312111/1‑101 (MQ=255)

                                                                           gcgcGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGt                     <  2:4311611/101‑1 (MQ=255)

                                                                           gcgcGTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGt                     >  2:4312112/1‑101 (MQ=255)

                                                                              cgTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGt                  >  1:4312118/1‑101 (MQ=255)

                                                                              cgTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGt                  >  2:4312119/1‑101 (MQ=255)

                                                                               gTGATGATGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGtt                 >  1:4312123/1‑101 (MQ=255)

                                                                                tgatgaTGCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGttt                >  2:4312127/1‑101 (MQ=255)

                                                                                  atgatgCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGc              <  1:4311552/101‑1 (MQ=255)

                                                                                  atgatgCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGc              <  1:4311368/101‑1 (MQ=255)

                                                                                  atgatgCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGc              >  2:4312131/1‑101 (MQ=255)

                                                                                    gatgCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGCCa            >  1:4312134/1‑101 (MQ=255)

                                                                                      tgCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCTGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGCCATc          <  1:4311671/101‑1 (MQ=255)

                                                                                      tgCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGCCATc          <  2:4311734/101‑1 (MQ=255)

                                                                                      tgCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGCCATc          >  2:4312138/1‑101 (MQ=255)

                                                                                       gCTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGCCATCt         >  1:4312140/1‑101 (MQ=255)

                                                                                        cTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGCCATCtt        <  1:4311330/101‑1 (MQ=255)

                                                                                        cTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGCCATCtt        >  1:4312145/1‑101 (MQ=255)

                                                                                        cTGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGAGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGCCATCtt        <  2:4311656/101‑1 (MQ=255)

                                                                                         tGGCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGCCATCTTg       >  1:4312148/1‑101 (MQ=255)

                                                                                          ggCTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGCCATCTTGt      >  2:4312151/1‑101 (MQ=255)

                                                                                            cTCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGCCATCTTGTTc    <  2:4311345/101‑1 (MQ=255)

                                                                                             tCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGCCATCTTGTTCa   <  2:4311618/101‑1 (MQ=255)

                                                                                             tCCGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGCCATCTTGTTCa   <  1:4311733/101‑1 (MQ=255)

                                                                                              ccGCTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGCCATCTTGTTCAt  >  1:4312154/1‑101 (MQ=255)

AGTGGCGCAGGTGGTGGCGGCAGGTCATGCCATCAGCCCGGATGCGGAAAACGCAGCAGTTATTTCCAAAATGCTGCGCGTGATGATGCTGGCTCCGTTCCTCATCCTGCTGGCGGCGCGTGTTAAACAGCTGTCTGGGGCGAACAGCGGCGAGAAAAGCAAAATCACTATTCCGTGGTTTGCCATCTTGTTCAT  >  NC_000913/2250337‑2250531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`