BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,274,125	T→C	Y588H (TAT→CAT)	yejA →	putative oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,274,125	0	T	C	100.0%	1303.7 / NA	313	Y588H (TAT→CAT)	yejA	putative oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (160/153); total (160/153)

Only 100 of 321 total aligned reads displayed.

CAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAAC  >  NC_000913/2274027‑2274222

 aaTGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATg                                                                                                >  1:4357656/1‑101 (MQ=255)

    gtgGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACg                                                                                             >  2:4357660/1‑101 (MQ=255)

     tgGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGt                                                                                            <  1:4357119/101‑1 (MQ=255)

         aCATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAAt                                                                                        >  2:4357666/1‑101 (MQ=255)

         aCATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAAt                                                                                        >  1:4357664/1‑101 (MQ=255)

         aCATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAAt                                                                                        <  1:4357001/101‑1 (MQ=255)

           aTGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATaa                                                                                      <  1:4356924/101‑1 (MQ=255)

            tGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATaaa                                                                                     <  2:4357113/101‑1 (MQ=255)

            tGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATaaa                                                                                     <  2:4357100/101‑1 (MQ=255)

            tGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATaaa                                                                                     >  1:4357670/1‑101 (MQ=255)

            tGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATaaa                                                                                     <  2:4356972/101‑1 (MQ=255)

              gCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATg                                                                                                >  2:4357676/1‑88 (MQ=255)

              gCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGc                                                                                   >  1:4357675/1‑101 (MQ=255)

               cGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCg                                                                                  <  1:4356771/101‑1 (MQ=255)

                ggAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATaaa                                                                                     >  1:4357683/1‑97 (MQ=255)

                ggAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCgg                                                                                 >  1:4357680/1‑101 (MQ=255)

                  aaGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGcc                                                                               <  1:4357185/101‑1 (MQ=255)

                   aGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCa                                                                              >  1:4357688/1‑101 (MQ=255)

                    gACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCaa                                                                             <  2:4357182/101‑1 (MQ=255)

                      ccGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAAc                                                                           <  2:4356818/101‑1 (MQ=255)

                       cGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACt                                                                          >  2:4357691/1‑101 (MQ=255)

                       cGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACt                                                                          <  1:4357289/101‑1 (MQ=255)

                        gTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTg                                                                         >  1:4357692/1‑101 (MQ=255)

                           tcGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCg                                                                      >  2:4357699/1‑101 (MQ=255)

                           tcGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCg                                                                      >  1:4357696/1‑101 (MQ=255)

                            cGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGt                                                                     <  1:4357366/101‑1 (MQ=255)

                            cGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGt                                                                     >  2:4357702/1‑101 (MQ=255)

                             gCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTc                                                                    <  2:4356943/101‑1 (MQ=255)

                               cTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCg                                                                  >  2:4357708/1‑101 (MQ=255)

                               cTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCg                                                                  >  1:4357707/1‑101 (MQ=255)

                                  gtggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCa                                                               <  1:4357310/101‑1 (MQ=255)

                                  gtggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCa                                                               <  1:4357127/101‑1 (MQ=255)

                                  gtggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCa                                                               >  1:4357713/1‑101 (MQ=255)

                                   tggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAg                                                              <  2:4357146/101‑1 (MQ=255)

                                   tggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAg                                                              >  2:4357715/1‑101 (MQ=255)

                                    gggATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGc                                                             <  1:4357041/101‑1 (MQ=255)

                                    gggATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGc                                                             <  2:4357198/101‑1 (MQ=255)

                                     ggATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCa                                                            >  2:4357718/1‑101 (MQ=255)

                                       aTAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCaaa                                                          <  1:4357237/101‑1 (MQ=255)

                                          aaTTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAg                                                       <  1:4357084/101‑1 (MQ=255)

                                            ttCTCCCAGCCGGCCGTGCGCCCCATCTATAGGCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTg                                                                         <  1:4356963/81‑1 (MQ=255)

                                             tctcCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGga                                                    >  1:4357722/1‑101 (MQ=255)

                                              ctcCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGgag                                                   >  2:4357724/1‑101 (MQ=255)

                                                cccAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGagag                                                 <  2:4357042/101‑1 (MQ=255)

                                                 ccAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGt                                                >  2:4357729/1‑101 (MQ=255)

                                                 ccAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGt                                                >  1:4357730/1‑101 (MQ=255)

                                                   aGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAg                                              >  1:4357737/1‑101 (MQ=255)

                                                   aGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAg                                              <  2:4357004/101‑1 (MQ=255)

                                                   aGCCGGCCGTGCGCCCCATCTAAAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAg                                              >  2:4357736/1‑101 (MQ=255)

                                                    gccggccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGa                                             <  2:4356689/101‑1 (MQ=255)

                                                     ccggccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGAt                                            >  1:4357742/1‑101 (MQ=255)

                                                      cggccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCaaa                                                                            <  2:4356981/68‑1 (MQ=255)

                                                      cggccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATg                                           <  1:4356989/101‑1 (MQ=255)

                                                       ggccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATgg                                          >  2:4357746/1‑101 (MQ=255)

                                                       ggccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATgg                                          >  1:4357749/1‑101 (MQ=255)

                                                        gccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATggg                                         >  2:4357750/1‑101 (MQ=255)

                                                             gcgcCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCtt                                    >  2:4357753/1‑101 (MQ=255)

                                                              cgcCCCATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTa                                   <  2:4357455/101‑1 (MQ=255)

                                                                ccccATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTAcc                                 >  2:4357756/1‑101 (MQ=255)

                                                                 cccATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCt                                <  1:4357393/101‑1 (MQ=255)

                                                                  ccATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTg                               >  2:4357761/1‑101 (MQ=255)

                                                                   cATCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGa                              >  1:4357762/1‑101 (MQ=255)

                                                                    aTCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGAt                             <  1:4357160/101‑1 (MQ=255)

                                                                    aTCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGAt                             >  2:4357768/1‑101 (MQ=255)

                                                                    aTCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGAt                             <  2:4357430/101‑1 (MQ=255)

                                                                    aTCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGAt                             <  1:4357262/101‑1 (MQ=255)

                                                                    aTCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGAt                             <  1:4357247/101‑1 (MQ=255)

                                                                     tCTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGAtt                            <  2:4357307/101‑1 (MQ=255)

                                                                      cTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTc                           <  2:4357249/101‑1 (MQ=255)

                                                                      cTATAGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTc                           >  1:4357770/1‑101 (MQ=255)

                                                                       tataGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCg                          <  1:4357014/101‑1 (MQ=255)

                                                                        ataGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGc                         <  2:4357211/101‑1 (MQ=255)

                                                                        ataGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGc                         >  1:4357775/1‑101 (MQ=255)

                                                                         taGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGcc                        <  2:4357224/101‑1 (MQ=255)

                                                                         taGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGcc                        <  1:4357547/101‑1 (MQ=255)

                                                                         taGCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGcc                        >  2:4357776/1‑101 (MQ=255)

                                                                           gCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGt                      >  2:4357781/1‑101 (MQ=255)

                                                                           gCCTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGt                      >  1:4357782/1‑101 (MQ=255)

                                                                             cTCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCt                    >  2:4357786/1‑101 (MQ=255)

                                                                              tCGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTg                   >  1:4357789/1‑101 (MQ=255)

                                                                               cGGTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGt                  <  2:4357496/101‑1 (MQ=255)

                                                                                ggTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGcc                        <  2:4357161/94‑1 (MQ=255)

                                                                                ggTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGtt                 >  2:4357794/1‑101 (MQ=255)

                                                                                ggTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGtt                 >  1:4357793/1‑101 (MQ=255)

                                                                                 gTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTg                >  2:4357799/1‑101 (MQ=255)

                                                                                 gTATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTg                <  1:4357221/101‑1 (MQ=255)

                                                                                  tATCGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGc               <  2:4357329/101‑1 (MQ=255)

                                                                                     cGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACt                                                                          <  2:4357058/39‑1 (MQ=255)

                                                                                     cGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTgg            >  2:4357806/1‑101 (MQ=255)

                                                                                     cGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTgg            >  1:4357804/1‑101 (MQ=255)

                                                                                     cGATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTgg            <  1:4357279/101‑1 (MQ=255)

                                                                                      gATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt           >  1:4357808/1‑101 (MQ=255)

                                                                                      gATACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt           <  1:4357098/101‑1 (MQ=255)

                                                                                        tACCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGa         <  1:4357301/101‑1 (MQ=255)

                                                                                         aCCTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGAt        >  1:4357813/1‑101 (MQ=255)

                                                                                           cTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATcc      >  2:4357815/1‑101 (MQ=255)

                                                                                           cTGGTGGCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATcc      <  1:4356917/101‑1 (MQ=255)

                                                                                            tggtggCATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc     <  2:4357375/101‑1 (MQ=255)

                                                                                              gtggcATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCaa   >  2:4357818/1‑101 (MQ=255)

                                                                                               tggcATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAAc  <  1:4357171/101‑1 (MQ=255)

CAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAAC  >  NC_000913/2274027‑2274222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`